RLG00000031141

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001372
Physical Location & Seq
Reverse (-)
2527573 .. 2529308
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031141

Sequence Viewer

Length: 1125 bp
ATGAAGCGGAAACGTGGACGTCCGCGTAAGGATGAGACTTTAGTTCATGGACAGAGCATGCCTGCAATACCTGGACCTGATAGTTTGAAGAAAAACAAGCACAGTGCGGGTACTAACGATGATGTCGAGGATGAAATGGTGGGTCGGGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTGAGGTAGAAGACACAGACACTCAGCTGAGGGCTGTTGTATTTCTACCAGGAAAGTTCACTCTAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTGATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCAACCTTCTGAGGTGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCCGTATACCTCAGTGTGGCATCCCGGAGTCTCTTAGAAGTCAATCTTTCTCTGGCATCTCGGAGTCTCTTAGCCAACGCATCTCGGAGTCTCTTAGAAAGTCGATCTTTCTCTGGCATCCCGGACAACAGTTGGAGCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGCTGTGGGTTCTGAAAGCCGTGCATTGTTTCAGGATGACGTAAAGAGCCTGAATTTTGAAGTCAACCAAACTCCTGTATTTGCTGAAGCTGCTTCTGCAATGGCTATCATACCAAGCGTTGAAACTACTGTGGATACTGAAAAGGGTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTACTGAACCTACACCAAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCTGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTAGACCTTGAGCTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTCACTGCCAGTAAACCAGTTGAGAATCTGATGGAAGAAGAGGCTGCACCAAAGCCGAGTCAGATGCCATTCCACCAGCACAATCTTGGATCACCTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

375

Amino Acids

40.77

Weight (kDa)

4.82

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
Acc36I ACCTGC 3 cut(s) 348, 427, 1027
AccI GTMKAC 1 cut(s) 448
AccII CGCG 1 cut(s) 25
AciI CCGC 3 cut(s) 7, 23, 107
AclI AACGTT 1 cut(s) 831
AclWI GGATC 3 cut(s) 905, 918, 1120
AcoI YGGCCR 1 cut(s) 368
AcsI RAATTY 3 cut(s) 435, 703, 918
AcuI CTGAAG 1 cut(s) 756
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 7 cut(s) 88, 167, 641, 710, 773, 836, 923
AjnI CCWGG 3 cut(s) 70, 247, 580
AluBI AGCT 5 cut(s) 226, 332, 653, 740, 991
AluI AGCT 5 cut(s) 226, 332, 653, 740, 991
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 5 cut(s) 29, 477, 513, 537, 890
AlwI GGATC 3 cut(s) 905, 918, 1120
AlwNI CAGNNNCTG 1 cut(s) 1019
AoxI GGCC 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 272, 740, 1067
ApoI RAATTY 3 cut(s) 435, 703, 918
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 3 cut(s) 367, 467, 564
AsuHPI GGTGA 2 cut(s) 415, 1107
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 927
BamHI GGATCC 1 cut(s) 910
BanII GRGCYC 1 cut(s) 993
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 993
BbvCI CCTCAGC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 284, 727, 1054
BccI CCATC 2 cut(s) 379, 1048
BceAI ACGGC 1 cut(s) 654
BcgI CGANNNNNNTGC 2 cut(s) 1069, 1103
BciT130I CCWGG 3 cut(s) 72, 249, 582
BciVI GTATCC 1 cut(s) 778
BcnI CCSGG 3 cut(s) 367, 467, 564
BcoDI GTCTC 5 cut(s) 29, 477, 513, 537, 890
BfaI CTAG 2 cut(s) 263, 980
BfmI CTRYAG 2 cut(s) 270, 420
BfuAI ACCTGC 3 cut(s) 348, 427, 1027
BfuI GTATCC 1 cut(s) 778
BisI GCNGC 3 cut(s) 273, 741, 1068
BlsI GCNGC 3 cut(s) 274, 742, 1069
Bme1390I CCNGG 6 cut(s) 72, 249, 367, 467, 564, 582
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 579, 912
BmrFI CCNGG 6 cut(s) 72, 249, 367, 467, 564, 582
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 6 cut(s) 169, 471, 507, 531, 568, 1077
BmuI ACTGGG 1 cut(s) 896
BpiI GAAGAC 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 1007
BpuMI CCSGG 3 cut(s) 367, 467, 564
BsaHI GRCGYC 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 891, 1032, 1040
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 1 cut(s) 756
BseBI CCWGG 3 cut(s) 72, 249, 582
BseGI GGATG 7 cut(s) 37, 136, 462, 559, 691, 937, 970
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 5 cut(s) 218, 236, 390, 467, 918
BseNI ACTGG 3 cut(s) 891, 1032, 1040
BseRI GAGGAG 1 cut(s) 993
BseXI GCAGC 3 cut(s) 284, 727, 1054
BsgI GTGCAG 1 cut(s) 1053
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 993
BsiSI CCGG 4 cut(s) 183, 367, 467, 564
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 5 cut(s) 29, 477, 513, 537, 890
BsmI GAATGC 1 cut(s) 1022
BsnI GGCC 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 4 cut(s) 349, 546, 910, 1112
BspACI CCGC 3 cut(s) 7, 23, 107
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 5 cut(s) 219, 235, 391, 466, 919
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 579, 912
BspMAI CTGCAG 2 cut(s) 274, 424
BspMI ACCTGC 3 cut(s) 348, 427, 1027
BspPI GGATC 3 cut(s) 905, 918, 1120
BsrDI GCAATG 1 cut(s) 756
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 891, 1032, 1040
BssAI RCCGGY 1 cut(s) 182
BssMI GATC 4 cut(s) 349, 546, 910, 1112
BssNAI GTATAC 1 cut(s) 449
BssNI GRCGYC 1 cut(s) 19
Bst1107I GTATAC 1 cut(s) 449
Bst2UI CCWGG 3 cut(s) 72, 249, 582
Bst4CI ACNGT 4 cut(s) 104, 573, 781, 901
Bst6I CTCTTC 1 cut(s) 1056
BstACI GRCGYC 1 cut(s) 19
BstAPI GCANNNNNTGC 2 cut(s) 1019, 1028
BstC8I GCNNGC 3 cut(s) 59, 63, 184
BstDEI CTNAG 8 cut(s) 222, 227, 399, 453, 476, 512, 536, 927
BstF5I GGATG 7 cut(s) 37, 136, 462, 559, 691, 937, 970
BstFNI CGCG 1 cut(s) 25
BstKTI GATC 4 cut(s) 352, 549, 913, 1115
BstMAI GTCTC 5 cut(s) 29, 477, 513, 537, 890
BstMBI GATC 4 cut(s) 349, 546, 910, 1112
BstMWI GCNNNNNNNGC 5 cut(s) 376, 740, 746, 1019, 1028
BstNI CCWGG 3 cut(s) 72, 249, 582
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 6 cut(s) 70, 247, 365, 465, 562, 580
BstSFI CTRYAG 2 cut(s) 270, 420
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 284, 727, 1054
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
BstZ17I GTATAC 1 cut(s) 449
Bsu36I CCTNAGG 1 cut(s) 927
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 1 cut(s) 370
BtsCI GGATG 7 cut(s) 37, 136, 462, 559, 691, 937, 970
BtsI GCAGTG 2 cut(s) 267, 1026
BtsIMutI CAGTG 4 cut(s) 109, 267, 461, 1026
BveI ACCTGC 3 cut(s) 348, 427, 1027
Cac8I GCNNGC 3 cut(s) 59, 63, 184
CaiI CAGNNNCTG 1 cut(s) 1019
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 111
CviAII CATG 3 cut(s) 47, 58, 605
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 8 cut(s) 222, 227, 399, 453, 476, 512, 536, 927
DpnI GATC 4 cut(s) 351, 548, 912, 1114
DpnII GATC 4 cut(s) 349, 546, 910, 1112
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 1056
EarI CTCTTC 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 756
Eco81I CCTNAGG 1 cut(s) 927
EcoICRI GAGCTC 1 cut(s) 991
EcoRII CCWGG 3 cut(s) 70, 247, 580
EcoT38I GRGCYC 1 cut(s) 993
FaeI CATG 3 cut(s) 50, 61, 608
FaiI YATR 8 cut(s) 48, 59, 357, 449, 606, 761, 884, 904
FalI AAGNNNNNCTT 4 cut(s) 472, 504, 533, 565
FatI CATG 3 cut(s) 46, 57, 604
FauI CCCGC 1 cut(s) 100
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 273, 741, 1068
FokI GGATG 7 cut(s) 44, 143, 449, 546, 698, 944, 977
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 3 cut(s) 273, 741, 1068
FspBI CTAG 2 cut(s) 263, 980
GluI GCNGC 3 cut(s) 273, 741, 1068
HaeIII GGCC 1 cut(s) 370
HapII CCGG 4 cut(s) 183, 367, 467, 564
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 3 cut(s) 50, 61, 608
HincII GTYRAC 2 cut(s) 337, 715
HindII GTYRAC 2 cut(s) 337, 715
HinfI GANTC 5 cut(s) 470, 506, 530, 1048, 1081
HpaII CCGG 4 cut(s) 183, 367, 467, 564
HphI GGTGA 2 cut(s) 415, 1107
Hpy166II GTNNAC 7 cut(s) 17, 258, 337, 449, 715, 829, 1037
Hpy188III TCNNGA 3 cut(s) 428, 683, 962
Hpy8I GTNNAC 7 cut(s) 17, 258, 337, 449, 715, 829, 1037
HpyAV CCTTC 2 cut(s) 161, 405
HpyCH4III ACNGT 4 cut(s) 104, 573, 781, 901
HpyCH4IV ACGT 4 cut(s) 13, 19, 690, 831
HpyF10VI GCNNNNNNNGC 5 cut(s) 376, 740, 746, 1019, 1028
HpyF3I CTNAG 8 cut(s) 222, 227, 399, 453, 476, 512, 536, 927
HpySE526I ACGT 4 cut(s) 13, 19, 690, 831
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 3 cut(s) 50, 61, 608
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 4 cut(s) 349, 546, 910, 1112
LmnI GCTCC 2 cut(s) 292, 577
Lsp1109I GCAGC 3 cut(s) 284, 727, 1054
LweI GCATC 6 cut(s) 169, 471, 507, 531, 568, 1077
MaeI CTAG 2 cut(s) 263, 980
MaeII ACGT 4 cut(s) 13, 19, 690, 831
MaeIII GTNAC 2 cut(s) 265, 383
MalI GATC 4 cut(s) 351, 548, 912, 1114
MboI GATC 4 cut(s) 349, 546, 910, 1112
MboII GAAGA 5 cut(s) 100, 182, 221, 1070, 1073
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 1 cut(s) 993
MluCI AATT 4 cut(s) 435, 703, 800, 918
MlyI GAGTC 4 cut(s) 479, 515, 539, 1090
MmeI TCCRAC 1 cut(s) 555
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 226, 332
MspI CCGG 4 cut(s) 183, 367, 467, 564
MspR9I CCNGG 6 cut(s) 72, 249, 367, 467, 564, 582
MunI CAATTG 1 cut(s) 800
Mva1269I GAATGC 1 cut(s) 1022
MvaI CCWGG 3 cut(s) 72, 249, 582
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 5 cut(s) 376, 740, 746, 1019, 1028
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 3 cut(s) 367, 467, 564
NdeII GATC 4 cut(s) 349, 546, 910, 1112
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 3 cut(s) 50, 61, 608
NlaIV GGNNCC 2 cut(s) 579, 912
NmeAIII GCCGAG 1 cut(s) 1104
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 61
PaeI GCATGC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 1022
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 1048
PflFI GACNNNGTC 1 cut(s) 616
PfoI TCCNGGA 2 cut(s) 465, 562
PkrI GCNGC 3 cut(s) 274, 742, 1069
PleI GAGTC 4 cut(s) 478, 514, 538, 1089
PpsI GAGTC 4 cut(s) 478, 514, 538, 1089
Psp124BI GAGCTC 1 cut(s) 993
Psp1406I AACGTT 1 cut(s) 831
Psp6I CCWGG 3 cut(s) 70, 247, 580
PspGI CCWGG 3 cut(s) 70, 247, 580
PspN4I GGNNCC 2 cut(s) 579, 912
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 274, 424
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 910
PsyI GACNNNGTC 1 cut(s) 616
PvuII CAGCTG 2 cut(s) 226, 332
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 3 cut(s) 273, 741, 1068
Sau3AI GATC 4 cut(s) 349, 546, 910, 1112
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 4 cut(s) 479, 515, 539, 1090
ScrFI CCNGG 6 cut(s) 72, 249, 367, 467, 564, 582
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 6 cut(s) 169, 471, 507, 531, 568, 1077
SfcI CTRYAG 2 cut(s) 270, 420
SinI GGWCC 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
SphI GCATGC 1 cut(s) 61
Sse9I AATT 4 cut(s) 435, 703, 800, 918
SsiI CCGC 3 cut(s) 7, 23, 107
SspMI CTAG 2 cut(s) 263, 980
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 6 cut(s) 70, 247, 365, 465, 562, 580
TaaI ACNGT 4 cut(s) 104, 573, 781, 901
TaiI ACGT 4 cut(s) 16, 22, 693, 834
TaqI TCGA 2 cut(s) 126, 545
TasI AATT 4 cut(s) 435, 703, 800, 918
TfiI GAWTC 1 cut(s) 1048
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 4 cut(s) 109, 274, 461, 1033
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 3 cut(s) 272, 740, 1067
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 5 cut(s) 17, 35, 147, 162, 645
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 4 cut(s) 109, 274, 461, 1033
Tth111I GACNNNGTC 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 3 cut(s) 435, 703, 918
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 1106
XmiI GTMKAC 1 cut(s) 448
XspI CTAG 2 cut(s) 263, 980
ZraI GACGTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.