Rw5G002840

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001372
Physical Location & Seq
Reverse (-)
2703916 .. 2705401
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002840.1

Sequence Viewer

Length: 1308 bp
ATGAATCAGAATAAAGAAACAAGCTCTTTATCTTCGCCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGTAAGGATGAGAATTTAAAAAACAAGCAGAGTGCGGGTACTAACGATGATGTCGAAGATGAAATGGTGGGTCGGGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTGAGGTAGAAGACACAGACACTCAGCTGAGGGCTGTTGTATTTCTACCAGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATATGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGCCCATCAGAGCAAAGTAACAGGCAACCTTCTGACATGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCTTCTCTTAGAAGTCGATCTTTCTCTGGCATCCCGGAGTCTCTTAGAAGCCAACCAACCTCTTTTGTAATTCCTCCGGTTGATAATTTGCCAAAAGATGATGCCCCTACCTTAGGAGGTAAAGTCATACCACAGCAACAGTTGGAGCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGATGTTGTTGAACAGGAAAAGGTGTTGGAGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAAACAGAAACATCATCTGAACCAATTGGTGAGGTACCAAGCATAGACACAGTACGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCCGTGCATTGGTTCAGGACGAAGTAAAGAGCCTGAATTTTGAAGTCAACCAAACTCCTGTATTTGCTGAAGCTGCTCCTGCAATGACTATCATACCAAGCGTTGAAACTAGTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCTACACCAAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCAGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTAGATCTTGAACTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTCACTGCCAGTAAACCAGTTGAGAATCTGATGGAAGAGCCCGCACCAAAGCCGAGTCAGGTGTTGGGGGGAGGTACTGTTGCATCTATGGGATCCATTCCTTCAGCCATGGGTGAAACTCCTTGTGGTGCTGTTGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

435

Amino Acids

46.72

Weight (kDa)

4.51

Isoelectric Point (pI)

56.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 3 cut(s) 348, 427, 1168
Acc65I GGTACC 1 cut(s) 763
AccB1I GGYRCC 1 cut(s) 763
AciI CCGC 4 cut(s) 55, 107, 367, 1206
AclI AACGTT 1 cut(s) 972
AclWI GGATC 6 cut(s) 785, 798, 1046, 1059, 1251, 1264
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 4 cut(s) 85, 435, 862, 1059
AcuI CTGAAG 2 cut(s) 915, 1251
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 4 cut(s) 112, 765, 783, 1240
AfiI CCNNNNNNNGG 2 cut(s) 554, 835
AgsI TTSAA 9 cut(s) 167, 650, 677, 803, 869, 932, 977, 1064, 1130
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 2 cut(s) 247, 589
AluBI AGCT 4 cut(s) 24, 226, 332, 899
AluI AGCT 4 cut(s) 24, 226, 332, 899
Alw26I GTCTC 2 cut(s) 486, 1031
AlwI GGATC 6 cut(s) 785, 798, 1046, 1059, 1251, 1264
AlwNI CAGNNNCTG 2 cut(s) 269, 1160
AoxI GGCC 1 cut(s) 681
ApeKI GCWGC 2 cut(s) 272, 899
ApoI RAATTY 4 cut(s) 85, 435, 862, 1059
Asp718I GGTACC 1 cut(s) 763
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 770, 1289
AxyI CCTNAGG 2 cut(s) 553, 807
BamHI GGATCC 3 cut(s) 790, 1051, 1256
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 1206
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
BbsI GAAGAC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 284, 886
BccI CCATC 2 cut(s) 379, 1189
BceAI ACGGC 1 cut(s) 813
BciT130I CCWGG 2 cut(s) 249, 591
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 2 cut(s) 486, 1031
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 2 cut(s) 936, 1121
BfmI CTRYAG 2 cut(s) 270, 420
BfuAI ACCTGC 3 cut(s) 348, 427, 1168
BglII AGATCT 2 cut(s) 40, 1123
BisI GCNGC 2 cut(s) 273, 900
BlsI GCNGC 2 cut(s) 274, 901
Bme1390I CCNGG 3 cut(s) 249, 476, 591
BmiI GGNNCC 5 cut(s) 588, 765, 792, 1053, 1258
BmrFI CCNGG 3 cut(s) 249, 476, 591
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 4 cut(s) 169, 480, 532, 1256
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 2 cut(s) 74, 785
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 1272
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 4 cut(s) 549, 579, 1227, 1257
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 835
Bse118I RCCGGY 2 cut(s) 182, 679
Bse1I ACTGG 5 cut(s) 263, 709, 1032, 1173, 1181
Bse21I CCTNAGG 2 cut(s) 553, 807
Bse3DI GCAATG 1 cut(s) 915
BseBI CCWGG 2 cut(s) 249, 591
BseDI CCNNGG 1 cut(s) 1272
BseGI GGATG 4 cut(s) 85, 471, 1078, 1111
BseLI CCNNNNNNNGG 2 cut(s) 554, 835
BseMI GCAATG 1 cut(s) 915
BseMII CTCAG 3 cut(s) 218, 236, 821
BseNI ACTGG 5 cut(s) 263, 709, 1032, 1173, 1181
BseRI GAGGAG 1 cut(s) 1134
BseXI GCAGC 2 cut(s) 284, 886
BshFI GGCC 1 cut(s) 683
BshNI GGYRCC 1 cut(s) 763
BsiSI CCGG 4 cut(s) 183, 476, 518, 680
BslI CCNNNNNNNGG 2 cut(s) 554, 835
BsmAI GTCTC 2 cut(s) 486, 1031
BsmI GAATGC 1 cut(s) 1163
BsnI GGCC 1 cut(s) 683
Bsp1286I GDGCHC 1 cut(s) 1206
Bsp143I GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
Bsp19I CCATGG 1 cut(s) 1272
BspACI CCGC 4 cut(s) 55, 107, 367, 1206
BspANI GGCC 1 cut(s) 683
BspCNI CTCAG 3 cut(s) 219, 235, 820
BspLI GGNNCC 5 cut(s) 588, 765, 792, 1053, 1258
BspMAI CTGCAG 2 cut(s) 274, 424
BspMI ACCTGC 3 cut(s) 348, 427, 1168
BspPI GGATC 6 cut(s) 785, 798, 1046, 1059, 1251, 1264
BspQI GCTCTTC 1 cut(s) 1194
BspT107I GGYRCC 1 cut(s) 763
BsrDI GCAATG 1 cut(s) 915
BsrFI RCCGGY 2 cut(s) 182, 679
BsrI ACTGG 5 cut(s) 263, 709, 1032, 1173, 1181
BssAI RCCGGY 2 cut(s) 182, 679
BssECI CCNNGG 1 cut(s) 1272
BssMI GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
BssNI GRCGYC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 1272
Bst2UI CCWGG 2 cut(s) 249, 591
Bst4CI ACNGT 4 cut(s) 582, 781, 1042, 1243
Bst6I CTCTTC 1 cut(s) 1194
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1169
BstBAI YACGTR 2 cut(s) 74, 785
BstC8I GCNNGC 3 cut(s) 184, 367, 1206
BstDEI CTNAG 6 cut(s) 222, 227, 449, 485, 553, 807
BstDSI CCRYGG 1 cut(s) 1272
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 4 cut(s) 85, 471, 1078, 1111
BstKTI GATC 6 cut(s) 43, 461, 793, 1054, 1126, 1259
BstMAI GTCTC 2 cut(s) 486, 1031
BstMBI GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
BstMWI GCNNNNNNNGC 4 cut(s) 899, 905, 1160, 1169
BstNI CCWGG 2 cut(s) 249, 591
BstSCI CCNGG 3 cut(s) 247, 474, 589
BstSFI CTRYAG 2 cut(s) 270, 420
BstSNI TACGTA 1 cut(s) 785
BstV1I GCAGC 2 cut(s) 284, 886
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 5 cut(s) 40, 790, 1051, 1123, 1256
BstYI RGATCY 5 cut(s) 40, 790, 1051, 1123, 1256
Bsu36I CCTNAGG 2 cut(s) 553, 807
BsuRI GGCC 1 cut(s) 683
BtgI CCRYGG 1 cut(s) 1272
BtsCI GGATG 4 cut(s) 85, 471, 1078, 1111
BtsI GCAGTG 2 cut(s) 267, 1167
BtsIMutI CAGTG 2 cut(s) 267, 1167
BveI ACCTGC 3 cut(s) 348, 427, 1168
Cac8I GCNNGC 3 cut(s) 184, 367, 1206
CaiI CAGNNNCTG 2 cut(s) 269, 1160
Cfr10I RCCGGY 2 cut(s) 182, 679
Csp6I GTAC 4 cut(s) 111, 764, 782, 1239
CviAII CATG 3 cut(s) 403, 614, 1273
CviQI GTAC 4 cut(s) 111, 764, 782, 1239
DdeI CTNAG 6 cut(s) 222, 227, 449, 485, 553, 807
DpnI GATC 6 cut(s) 42, 460, 792, 1053, 1125, 1258
DpnII GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
DraI TTTAAA 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 1194
EarI CTCTTC 1 cut(s) 1194
Eco105I TACGTA 1 cut(s) 785
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 1206
Eco57I CTGAAG 2 cut(s) 915, 1251
Eco81I CCTNAGG 2 cut(s) 553, 807
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 247, 589
EcoT14I CCWWGG 1 cut(s) 1272
EcoT38I GRGCYC 1 cut(s) 1206
ErhI CCWWGG 1 cut(s) 1272
FaeI CATG 3 cut(s) 406, 617, 1276
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FatI CATG 3 cut(s) 402, 613, 1272
FauI CCCGC 3 cut(s) 100, 374, 1213
Fnu4HI GCNGC 2 cut(s) 273, 900
FokI GGATG 4 cut(s) 92, 458, 1085, 1118
FriOI GRGCYC 1 cut(s) 1206
Fsp4HI GCNGC 2 cut(s) 273, 900
FspBI CTAG 2 cut(s) 936, 1121
GluI GCNGC 2 cut(s) 273, 900
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 683
HapII CCGG 4 cut(s) 183, 476, 518, 680
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 3 cut(s) 406, 617, 1276
HincII GTYRAC 2 cut(s) 337, 874
HindII GTYRAC 2 cut(s) 337, 874
HinfI GANTC 5 cut(s) 4, 479, 725, 1189, 1219
HpaII CCGG 4 cut(s) 183, 476, 518, 680
HphI GGTGA 2 cut(s) 770, 1289
Hpy166II GTNNAC 7 cut(s) 65, 71, 258, 337, 874, 970, 1178
Hpy188I TCNGA 9 cut(s) 9, 195, 376, 400, 631, 748, 823, 1084, 1194
Hpy188III TCNNGA 4 cut(s) 428, 842, 1103, 1127
Hpy8I GTNNAC 7 cut(s) 65, 71, 258, 337, 874, 970, 1178
HpyAV CCTTC 4 cut(s) 161, 405, 453, 1275
HpyCH4III ACNGT 4 cut(s) 582, 781, 1042, 1243
HpyCH4IV ACGT 5 cut(s) 61, 67, 73, 784, 972
HpyF10VI GCNNNNNNNGC 4 cut(s) 899, 905, 1160, 1169
HpyF3I CTNAG 6 cut(s) 222, 227, 449, 485, 553, 807
HpySE526I ACGT 5 cut(s) 61, 67, 73, 784, 972
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 3 cut(s) 406, 617, 1276
KpnI GGTACC 1 cut(s) 767
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
LguI GCTCTTC 1 cut(s) 1194
LmnI GCTCC 3 cut(s) 292, 586, 907
Lsp1109I GCAGC 2 cut(s) 284, 886
LweI GCATC 4 cut(s) 169, 480, 532, 1256
MaeI CTAG 2 cut(s) 936, 1121
MaeII ACGT 5 cut(s) 61, 67, 73, 784, 972
MaeIII GTNAC 2 cut(s) 265, 383
MalI GATC 6 cut(s) 42, 460, 792, 1053, 1125, 1258
MboI GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
MboII GAAGA 5 cut(s) 24, 140, 182, 221, 1211
MfeI CAATTG 2 cut(s) 753, 941
MflI RGATCY 5 cut(s) 40, 790, 1051, 1123, 1256
MhlI GDGCHC 1 cut(s) 1206
MluCI AATT 9 cut(s) 85, 435, 510, 526, 753, 798, 862, 941, 1059
MlyI GAGTC 2 cut(s) 488, 1228
MmeI TCCRAC 2 cut(s) 564, 645
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 2 cut(s) 89, 297
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 226, 332
MspI CCGG 4 cut(s) 183, 476, 518, 680
MspR9I CCNGG 3 cut(s) 249, 476, 591
MunI CAATTG 2 cut(s) 753, 941
Mva1269I GAATGC 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 249, 591
MwoI GCNNNNNNNGC 4 cut(s) 899, 905, 1160, 1169
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 1 cut(s) 1272
NdeII GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 3 cut(s) 406, 617, 1276
NlaIV GGNNCC 5 cut(s) 588, 765, 792, 1053, 1258
NmeAIII GCCGAG 1 cut(s) 1242
NmuCI GTSAC 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 1194
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 1163
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 3 cut(s) 4, 725, 1189
PflFI GACNNNGTC 1 cut(s) 625
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 2 cut(s) 274, 901
PleI GAGTC 2 cut(s) 487, 1227
PpsI GAGTC 2 cut(s) 487, 1227
Ppu21I YACGTR 2 cut(s) 74, 785
Psp1406I AACGTT 1 cut(s) 972
Psp6I CCWGG 2 cut(s) 247, 589
PspGI CCWGG 2 cut(s) 247, 589
PspN4I GGNNCC 5 cut(s) 588, 765, 792, 1053, 1258
PstI CTGCAG 2 cut(s) 274, 424
PstNI CAGNNNCTG 2 cut(s) 269, 1160
PsuI RGATCY 5 cut(s) 40, 790, 1051, 1123, 1256
PsyI GACNNNGTC 1 cut(s) 625
PvuII CAGCTG 2 cut(s) 226, 332
RsaI GTAC 4 cut(s) 112, 765, 783, 1240
RsaNI GTAC 4 cut(s) 111, 764, 782, 1239
RseI CAYNNNNRTG 1 cut(s) 354
SapI GCTCTTC 1 cut(s) 1194
SaqAI TTAA 2 cut(s) 89, 297
SatI GCNGC 2 cut(s) 273, 900
Sau3AI GATC 6 cut(s) 40, 458, 790, 1051, 1123, 1256
SchI GAGTC 2 cut(s) 488, 1228
ScrFI CCNGG 3 cut(s) 249, 476, 591
SduI GDGCHC 1 cut(s) 1206
SfaNI GCATC 4 cut(s) 169, 480, 532, 1256
SfcI CTRYAG 2 cut(s) 270, 420
SmiMI CAYNNNNRTG 1 cut(s) 354
SnaBI TACGTA 1 cut(s) 785
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 9 cut(s) 85, 435, 510, 526, 753, 798, 862, 941, 1059
SsiI CCGC 4 cut(s) 55, 107, 367, 1206
SspMI CTAG 2 cut(s) 936, 1121
StyD4I CCNGG 3 cut(s) 247, 474, 589
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 4 cut(s) 582, 781, 1042, 1243
TaiI ACGT 5 cut(s) 64, 70, 76, 787, 975
TaqI TCGA 2 cut(s) 126, 457
TasI AATT 9 cut(s) 85, 435, 510, 526, 753, 798, 862, 941, 1059
TfiI GAWTC 3 cut(s) 4, 725, 1189
Tru1I TTAA 2 cut(s) 89, 297
Tru9I TTAA 2 cut(s) 89, 297
TscAI CASTG 2 cut(s) 274, 1174
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 272, 899
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 5 cut(s) 17, 65, 147, 162, 419
TspRI CASTG 2 cut(s) 274, 1174
Tth111I GACNNNGTC 1 cut(s) 625
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 4 cut(s) 85, 435, 862, 1059
XspI CTAG 2 cut(s) 936, 1121
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.