RLG00000031140

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001372
Physical Location & Seq
Forward (+)
2518785 .. 2524436
5652 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031140

Sequence Viewer

Length: 1698 bp
ATGTTCATAGATATTGTATTTGCAGAGACCCCTATTTCCATCTGTTTCGGGCCAATTCTCCATTTCCCGCATAAACAGAATCTCACTCCCAAACCCAAAAAACTGAATTCTGAGAAGTTCCTCGCCACCCACTTGGGAAATTTTGAAAACCAGGGCATCTTTCTCTGCAGTATGAATCAGAGTCAAGGAACAAGCTCTTTATCTTTGCCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGAAAGGATGAGAATTTAGTTCACGGAGAGAGCATTCCTGCAATGCCAGGACCTGATAGTTTGAAGAAAAACAAACAGAGTGCGGGTACTAACGATGATGGTGAAGATGAAATGGTGGGTCGAGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTAAGGTAGAAGACACCGACACTCAGCTGAGGGGTGTTGTATTTCTTCCGGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTGATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCCACCTGTTGAGGTGAAAAAGCAAATACCTGCAATTCCAGACCAAATTCCTCCCCGTATTCCTCAGTGTGGCATCCTGGAGACTCTTAGAAGTCAATCTTTCTCTGGCATCCCAGAGTCTCTTCAAAGCCAACGCATCCAGGAGTCTCTTAGAAGTCAATCTTTCCCTGGCATCCCGGAGTCTTTCAGCCAACCATCCTCTTTTGTAAATCCTCCGGTTGATAATTTGCCAAAGAATGATGCCCCTGTCTTGGGAGGTAAAGTCACACCACAGCAACCTTTGGATCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGAGGAAGTTGAACCGGCCATAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAGACAGAAACATCATCTGAACCAATTGGTGAGGTACTAAGCATAGACACAGTATGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCAGTGCATTGGTTCAGGATGACGTAAAGAGCATGAATTTTGAGTCAATTCCTGTATTTGCTGAAGCTGCTCCTGCAATGGCTACCATACAAAACGTTGAAACTGTATGCAAGGCTCCTCAAATTCAACCTCAGACTGTGGATACTGAAAAGACTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCCGCAGCCAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCTGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAGAGAGCCTACATCTTGAGCTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTTGCAGCCAGTAAACCAGTTGACAATCTGACGGAAGAAGAGGATGCACCAAAGCCGAGTCAGGTGTTAGGGGCCGAGGCTGCATCTGTAGGATCCATTCCTTCAGCCATGGGTGAACCTCCTTCTGGTGCTGTTGACAATATGGAATGTGATATTGCAGATGCCATTTCACCTGCACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

60.66

Weight (kDa)

4.52

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1693
AatII GACGTC 1 cut(s) 241
Acc36I ACCTGC 4 cut(s) 567, 646, 1516, 1693
AciI CCGC 4 cut(s) 68, 226, 326, 1356
AclI AACGTT 2 cut(s) 1227, 1320
AclWI GGATC 8 cut(s) 887, 900, 1091, 1104, 1394, 1407, 1599, 1612
AcoI YGGCCR 2 cut(s) 587, 987
AcsI RAATTY 7 cut(s) 106, 139, 256, 654, 1168, 1254, 1407
AcuI CTGAAG 2 cut(s) 1215, 1599
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 2 cut(s) 331, 1071
AfiI CCNNNNNNNGG 4 cut(s) 677, 859, 860, 1637
AjnI CCWGG 6 cut(s) 150, 289, 684, 747, 775, 895
AluBI AGCT 5 cut(s) 195, 445, 551, 1199, 1480
AluI AGCT 5 cut(s) 195, 445, 551, 1199, 1480
Alw21I GWGCWC 1 cut(s) 1482
Alw26I GTCTC 5 cut(s) 20, 683, 732, 759, 1379
AlwI GGATC 8 cut(s) 887, 900, 1091, 1104, 1394, 1407, 1599, 1612
AlwNI CAGNNNCTG 3 cut(s) 296, 488, 1508
AoxI GGCC 5 cut(s) 50, 587, 608, 987, 1584
ApeKI GCWGC 5 cut(s) 491, 1199, 1358, 1517, 1592
ApoI RAATTY 7 cut(s) 106, 139, 256, 654, 1168, 1254, 1407
AspS9I GGNCC 3 cut(s) 50, 293, 1584
AsuC2I CCSGG 3 cut(s) 468, 586, 785
AsuHPI GGTGA 5 cut(s) 356, 634, 1076, 1637, 1674
AvaII GGWCC 1 cut(s) 293
AxyI CCTNAGG 2 cut(s) 1113, 1416
BamHI GGATCC 4 cut(s) 892, 1096, 1399, 1604
BanII GRGCYC 1 cut(s) 1482
BarI GAAGNNNNNNTAC 2 cut(s) 528, 560
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 1482
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 5 cut(s) 503, 1186, 1370, 1529, 1579
BccI CCATC 4 cut(s) 47, 335, 598, 811
BciT130I CCWGG 6 cut(s) 152, 291, 686, 749, 777, 897
BciVI GTATCC 1 cut(s) 1267
BcnI CCSGG 3 cut(s) 468, 586, 785
BcoDI GTCTC 5 cut(s) 20, 683, 732, 759, 1379
BfmI CTRYAG 3 cut(s) 166, 489, 1599
BfuAI ACCTGC 4 cut(s) 567, 646, 1516, 1693
BfuI GTATCC 1 cut(s) 1267
BglII AGATCT 1 cut(s) 211
BisI GCNGC 5 cut(s) 492, 1200, 1359, 1518, 1593
BlsI GCNGC 5 cut(s) 493, 1201, 1360, 1519, 1594
Bme1390I CCNGG 9 cut(s) 152, 291, 468, 586, 686, 749, 777, 785, 897
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 3 cut(s) 50, 293, 1584
BmiI GGNNCC 6 cut(s) 894, 1098, 1248, 1401, 1585, 1606
BmrFI CCNGG 9 cut(s) 152, 291, 468, 586, 686, 749, 777, 785, 897
BmrI ACTGGG 1 cut(s) 1385
BmuI ACTGGG 1 cut(s) 1385
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 2 cut(s) 465, 707
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 1496
BpuMI CCSGG 3 cut(s) 468, 586, 785
BsaHI GRCGYC 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 4 cut(s) 151, 775, 1587, 1620
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 4 cut(s) 677, 859, 860, 1637
Bse118I RCCGGY 2 cut(s) 401, 985
Bse1I ACTGG 6 cut(s) 482, 1015, 1362, 1380, 1521, 1529
Bse21I CCTNAGG 2 cut(s) 1113, 1416
Bse3DI GCAATG 2 cut(s) 291, 1215
BseBI CCWGG 6 cut(s) 152, 291, 686, 749, 777, 897
BseDI CCNNGG 4 cut(s) 151, 775, 1587, 1620
BseLI CCNNNNNNNGG 4 cut(s) 677, 859, 860, 1637
BseMI GCAATG 2 cut(s) 291, 1215
BseMII CTCAG 7 cut(s) 102, 437, 455, 686, 1127, 1277, 1407
BseNI ACTGG 6 cut(s) 482, 1015, 1362, 1380, 1521, 1529
BseRI GAGGAG 2 cut(s) 1239, 1482
BseXI GCAGC 5 cut(s) 503, 1186, 1370, 1529, 1579
BsgI GTGCAG 1 cut(s) 1671
BshFI GGCC 5 cut(s) 52, 589, 610, 989, 1586
BsiHKAI GWGCWC 1 cut(s) 1482
BsiSI CCGG 6 cut(s) 402, 467, 586, 785, 824, 986
BslI CCNNNNNNNGG 4 cut(s) 677, 859, 860, 1637
BsmAI GTCTC 5 cut(s) 20, 683, 732, 759, 1379
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 5 cut(s) 52, 589, 610, 989, 1586
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 1482
Bsp143I GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
Bsp19I CCATGG 1 cut(s) 1620
BspACI CCGC 4 cut(s) 68, 226, 326, 1356
BspANI GGCC 5 cut(s) 52, 589, 610, 989, 1586
BspCNI CTCAG 7 cut(s) 103, 438, 454, 685, 1126, 1276, 1408
BspLI GGNNCC 6 cut(s) 894, 1098, 1248, 1401, 1585, 1606
BspMAI CTGCAG 2 cut(s) 170, 493
BspMI ACCTGC 4 cut(s) 567, 646, 1516, 1693
BspPI GGATC 8 cut(s) 887, 900, 1091, 1104, 1394, 1407, 1599, 1612
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 2 cut(s) 291, 1215
BsrFI RCCGGY 2 cut(s) 401, 985
BsrI ACTGG 6 cut(s) 482, 1015, 1362, 1380, 1521, 1529
BssAI RCCGGY 2 cut(s) 401, 985
BssECI CCNNGG 4 cut(s) 151, 775, 1587, 1620
BssMI GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
BssNI GRCGYC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 1620
Bst2UI CCWGG 6 cut(s) 152, 291, 686, 749, 777, 897
Bst4CI ACNGT 4 cut(s) 1087, 1237, 1270, 1390
Bst6I CTCTTC 2 cut(s) 735, 1545
BstACI GRCGYC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 1508
BstC8I GCNNGC 1 cut(s) 403
BstDSI CCRYGG 1 cut(s) 1620
BstKTI GATC 6 cut(s) 214, 571, 895, 1099, 1402, 1607
BstMAI GTCTC 5 cut(s) 20, 683, 732, 759, 1379
BstMBI GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
BstMWI GCNNNNNNNGC 7 cut(s) 595, 1199, 1205, 1355, 1508, 1517, 1592
BstNI CCWGG 6 cut(s) 152, 291, 686, 749, 777, 897
BstSCI CCNGG 9 cut(s) 150, 289, 466, 584, 684, 747, 775, 783, 895
BstSFI CTRYAG 3 cut(s) 166, 489, 1599
BstV1I GCAGC 5 cut(s) 503, 1186, 1370, 1529, 1579
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 5 cut(s) 211, 892, 1096, 1399, 1604
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 5 cut(s) 211, 892, 1096, 1399, 1604
Bsu36I CCTNAGG 2 cut(s) 1113, 1416
BsuI GTATCC 1 cut(s) 1267
BsuRI GGCC 5 cut(s) 52, 589, 610, 989, 1586
BtgI CCRYGG 1 cut(s) 1620
BtsI GCAGTG 2 cut(s) 486, 1141
BtsIMutI CAGTG 3 cut(s) 486, 680, 1141
BveI ACCTGC 4 cut(s) 567, 646, 1516, 1693
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 3 cut(s) 296, 488, 1508
Cfr10I RCCGGY 2 cut(s) 401, 985
Cfr13I GGNCC 3 cut(s) 50, 293, 1584
Csp6I GTAC 2 cut(s) 330, 1070
CviAII CATG 3 cut(s) 920, 1165, 1621
CviQI GTAC 2 cut(s) 330, 1070
DpnI GATC 6 cut(s) 213, 570, 894, 1098, 1401, 1606
DpnII GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
EaeI YGGCCR 2 cut(s) 587, 987
Eam1104I CTCTTC 2 cut(s) 735, 1545
EarI CTCTTC 2 cut(s) 735, 1545
Ecl136II GAGCTC 1 cut(s) 1480
Eco130I CCWWGG 1 cut(s) 1620
Eco24I GRGCYC 1 cut(s) 1482
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 1480
Eco57I CTGAAG 2 cut(s) 1215, 1599
Eco81I CCTNAGG 2 cut(s) 1113, 1416
EcoICRI GAGCTC 1 cut(s) 1480
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 106
EcoRII CCWGG 6 cut(s) 150, 289, 684, 747, 775, 895
EcoT14I CCWWGG 1 cut(s) 1620
EcoT38I GRGCYC 1 cut(s) 1482
ErhI CCWWGG 1 cut(s) 1620
FaeI CATG 3 cut(s) 923, 1168, 1624
FalI AAGNNNNNCTT 4 cut(s) 691, 723, 754, 786
FatI CATG 3 cut(s) 919, 1164, 1620
FauI CCCGC 3 cut(s) 75, 319, 1363
Fnu4HI GCNGC 5 cut(s) 492, 1200, 1359, 1518, 1593
FriOI GRGCYC 1 cut(s) 1482
Fsp4HI GCNGC 5 cut(s) 492, 1200, 1359, 1518, 1593
GluI GCNGC 5 cut(s) 492, 1200, 1359, 1518, 1593
GsuI CTGGAG 2 cut(s) 465, 707
HaeIII GGCC 5 cut(s) 52, 589, 610, 989, 1586
HapII CCGG 6 cut(s) 402, 467, 586, 785, 824, 986
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 3 cut(s) 923, 1168, 1624
HincII GTYRAC 3 cut(s) 556, 1534, 1648
HindII GTYRAC 3 cut(s) 556, 1534, 1648
HpaII CCGG 6 cut(s) 402, 467, 586, 785, 824, 986
HphI GGTGA 5 cut(s) 356, 634, 1076, 1637, 1674
Hpy188III TCNNGA 4 cut(s) 647, 1148, 1451, 1475
HpyAV CCTTC 3 cut(s) 380, 1623, 1644
HpyCH4III ACNGT 4 cut(s) 1087, 1237, 1270, 1390
HpyCH4IV ACGT 5 cut(s) 232, 238, 1155, 1227, 1320
HpyF10VI GCNNNNNNNGC 7 cut(s) 595, 1199, 1205, 1355, 1508, 1517, 1592
HpySE526I ACGT 5 cut(s) 232, 238, 1155, 1227, 1320
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 3 cut(s) 923, 1168, 1624
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Kzo9I GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
LmnI GCTCC 3 cut(s) 511, 1207, 1252
Lsp1109I GCAGC 5 cut(s) 503, 1186, 1370, 1529, 1579
MaeII ACGT 5 cut(s) 232, 238, 1155, 1227, 1320
MaeIII GTNAC 3 cut(s) 484, 602, 871
MalI GATC 6 cut(s) 213, 570, 894, 1098, 1401, 1606
MboI GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
MboII GAAGA 8 cut(s) 319, 359, 401, 440, 455, 722, 1559, 1562
MfeI CAATTG 2 cut(s) 1059, 1289
MflI RGATCY 5 cut(s) 211, 892, 1096, 1399, 1604
MhlI GDGCHC 1 cut(s) 1482
MlyI GAGTC 8 cut(s) 190, 685, 734, 761, 797, 1184, 1279, 1579
MmeI TCCRAC 1 cut(s) 951
MroNI GCCGGC 1 cut(s) 401
MseI TTAA 2 cut(s) 420, 516
MspA1I CMGCKG 2 cut(s) 445, 551
MspI CCGG 6 cut(s) 402, 467, 586, 785, 824, 986
MspR9I CCNGG 9 cut(s) 152, 291, 468, 586, 686, 749, 777, 785, 897
MunI CAATTG 2 cut(s) 1059, 1289
Mva1269I GAATGC 1 cut(s) 276
MvaI CCWGG 6 cut(s) 152, 291, 686, 749, 777, 897
MwoI GCNNNNNNNGC 7 cut(s) 595, 1199, 1205, 1355, 1508, 1517, 1592
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 3 cut(s) 468, 586, 785
NcoI CCATGG 1 cut(s) 1620
NdeII GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
NgoMIV GCCGGC 1 cut(s) 401
NlaIII CATG 3 cut(s) 923, 1168, 1624
NlaIV GGNNCC 6 cut(s) 894, 1098, 1248, 1401, 1585, 1606
NmeAIII GCCGAG 2 cut(s) 1593, 1612
NmuCI GTSAC 2 cut(s) 484, 871
PaqCI CACCTGC 1 cut(s) 1693
PctI GAATGC 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 403
PfeI GAWTC 3 cut(s) 79, 175, 1031
PflFI GACNNNGTC 1 cut(s) 931
PfoI TCCNGGA 5 cut(s) 466, 684, 747, 783, 895
PkrI GCNGC 5 cut(s) 493, 1201, 1360, 1519, 1594
PleI GAGTC 8 cut(s) 189, 685, 733, 760, 796, 1183, 1279, 1578
PpsI GAGTC 8 cut(s) 189, 685, 733, 760, 796, 1183, 1279, 1578
PpuMI RGGWCCY 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 1482
Psp1406I AACGTT 2 cut(s) 1227, 1320
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 6 cut(s) 150, 289, 684, 747, 775, 895
PspGI CCWGG 6 cut(s) 150, 289, 684, 747, 775, 895
PspN4I GGNNCC 6 cut(s) 894, 1098, 1248, 1401, 1585, 1606
PspPI GGNCC 3 cut(s) 50, 293, 1584
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 2 cut(s) 170, 493
PstNI CAGNNNCTG 3 cut(s) 296, 488, 1508
PsuI RGATCY 5 cut(s) 211, 892, 1096, 1399, 1604
PsyI GACNNNGTC 1 cut(s) 931
PvuII CAGCTG 2 cut(s) 445, 551
RsaI GTAC 2 cut(s) 331, 1071
RsaNI GTAC 2 cut(s) 330, 1070
SacI GAGCTC 1 cut(s) 1482
SaqAI TTAA 2 cut(s) 420, 516
SatI GCNGC 5 cut(s) 492, 1200, 1359, 1518, 1593
Sau3AI GATC 6 cut(s) 211, 568, 892, 1096, 1399, 1604
Sau96I GGNCC 3 cut(s) 50, 293, 1584
SchI GAGTC 8 cut(s) 190, 685, 734, 761, 797, 1184, 1279, 1579
ScrFI CCNGG 9 cut(s) 152, 291, 468, 586, 686, 749, 777, 785, 897
SduI GDGCHC 1 cut(s) 1482
SfcI CTRYAG 3 cut(s) 166, 489, 1599
SinI GGWCC 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 1475
SmoI CTYRAG 1 cut(s) 1475
SsiI CCGC 4 cut(s) 68, 226, 326, 1356
SstI GAGCTC 1 cut(s) 1482
StyD4I CCNGG 9 cut(s) 150, 289, 466, 584, 684, 747, 775, 783, 895
StyI CCWWGG 1 cut(s) 1620
TaaI ACNGT 4 cut(s) 1087, 1237, 1270, 1390
TaiI ACGT 5 cut(s) 235, 241, 1158, 1230, 1323
TaqI TCGA 1 cut(s) 364
TfiI GAWTC 3 cut(s) 79, 175, 1031
Tru1I TTAA 2 cut(s) 420, 516
Tru9I TTAA 2 cut(s) 420, 516
TscAI CASTG 3 cut(s) 493, 680, 1141
TseFI GTSAC 2 cut(s) 484, 871
TseI GCWGC 5 cut(s) 491, 1199, 1358, 1517, 1592
Tsp45I GTSAC 2 cut(s) 484, 871
TspDTI ATGAA 6 cut(s) 188, 236, 366, 381, 960, 1181
TspGWI ACGGA 2 cut(s) 282, 1559
TspRI CASTG 3 cut(s) 493, 680, 1141
Tth111I GACNNNGTC 1 cut(s) 931
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 7 cut(s) 106, 139, 256, 654, 1168, 1254, 1407
ZraI GACGTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.