pycom10g27070

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001372
Physical Location & Seq
Reverse (-)
28150024 .. 28154802
4779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27070.1

Sequence Viewer

Length: 1584 bp
ATGGGCCGGTCAAGAAAAATTGATTTTCCCACCATTGCCGCGGATTTGGATTTCCTTGATTTGATTCCACCTCTCGTTTCCTCGCCGTCGCCGGCCGCCGTCCGCCGCATCGTGAAAGAATCGAGGTCTACAGCTCAATCCTTCATTCCGATCATAGACTTGGGGAAAACTTTAAAACGTGAAATCTGTCATCATGAATCAACAGAGTCAAGGAACGGGCTCTCTATCTTCGCCAGACCCCCCTATGAAGCGAAAACGTGGACGTCCGCTTCGAAGAAAACCAAACAGAGTGCAAGTACAAGTGATGATGTTGAGAATGAAATGGTAGGCCAGGTAGTCACTGGCGTGATTGAGGGGTCATTTGATGCTGGATATCTTCTGAATGTTAGGGTCGGAGACAGTGACACTCAGCTGAGGGGTGTTGTGTTTCTACAGGAGAAAATCACTCCCATTACTCCAGCAAATGACATTGCTCCTCATATTATGATGTGCAAAAGAAGAGAAATTCCCATACCACCTCTCAACCCACAATCTCAGCGTTATGAGTCTGCCCGCCCATCAGAACAAAGTAATAGGCAACCTGTTGAGGTGAAACCCCAAATACCTACAGTTCCTAACCAAATTCAATCCTCTGTCCCTCAGTCTGGAATCCTGGAGTCACTTGAAAACAAACCTTTCTCTTTTGTGACTCCTCCGGTTGTGAACTTGCTGAAGAATGATGCAGTCCTGCCCTTGGGAGGAAAAGTCATGCCACAGCAATTTCCAGAACCTGGAACTGATAACCAATCTGCCCCTTCTATGCCCAAGCCAGAGCATAATAACGTTGTTCAACAGGAGAAAGTGCTGGAGGAAGTTGAAGCTGCCACAATAATGGAAGGGCCAAATGGTGTAGAGGCCAATAAGGCATCAAAAACAGAAACAGCAGCTGAACCAATTGCTGACCTACCAGGTGTTGAACCCGTATGTAAGGCTCCTGAAATTCAAATTCAGGCTGCGGGTTCTGAAAAGAGTGCGTCGGCTCCTTATGAAGTAAAGAGCTTCAGACTTGAACTCAACCAAATGCCTGTATTCGGTGAACCCAGAGTGACCACTGTCATGCCAGTTGTCGAACTCAACCACATGCCTGTATTTGCTGAATCCGCAAGAATCGCTGCCATGCCAGTCATTGAAACTGAAGGTAAGGACAATCAAATTCAACCTCAGGCTGTGGATTCTGAAAAGAGCGCATTGGTTCATGATGGTGCAAAGAGCCCGAATCTTGAGCTCAACCAAACTCCTGTATTTGCTGAACCTGCCCCTGTTGCTCTCATACCAGGCATTGAAACCGTATGCAAGGATTCTCAAATTCAACCTCCAGATGTCAGTCCTGAAACGAGTTCATTGGTTCATGATGCGGTAAAGAGCGTGGACATAGAACTCAACCAAACTCCTTTTGCTGCTCCGGTTAGTATTCCATCCGAATCAAAGATTGCTGCAGAAGGATCTATTCCTCTGGGAACTGCCGAACCTCAAATCCCTACTTCTGATGGCATTAGCAACATGGAGTGTGACATCGAAGATGTCATTCCAGCTGCACAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

528

Amino Acids

56.73

Weight (kDa)

4.78

Isoelectric Point (pI)

64.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 1300
AccB7I CCANNNNNTGG 1 cut(s) 770
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 41
AclI AACGTT 1 cut(s) 822
AclWI GGATC 1 cut(s) 1489
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 6 cut(s) 504, 621, 978, 984, 1191, 1344
AcuI CTGAAG 3 cut(s) 731, 1024, 1194
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 5 cut(s) 644, 733, 737, 770, 1070
AjnI CCWGG 5 cut(s) 330, 651, 769, 946, 1312
AleI CACNNNNGTG 1 cut(s) 344
AloI GAACNNNNNNTCC 2 cut(s) 1497, 1529
AluBI AGCT 7 cut(s) 134, 412, 860, 926, 1038, 1264, 1571
AluI AGCT 7 cut(s) 134, 412, 860, 926, 1038, 1264, 1571
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 1 cut(s) 1489
AlwNI CAGNNNCTG 2 cut(s) 770, 926
AoxI GGCC 5 cut(s) 4, 93, 328, 878, 894
ApeKI GCWGC 7 cut(s) 860, 923, 992, 1151, 1436, 1472, 1571
ApoI RAATTY 6 cut(s) 504, 621, 978, 984, 1191, 1344
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspLEI GCGC 1 cut(s) 1226
AspS9I GGNCC 2 cut(s) 4, 878
AsuHPI GGTGA 2 cut(s) 601, 1085
AsuII TTCGAA 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 1200
BanII GRGCYC 3 cut(s) 222, 1253, 1266
Bbv12I GWGCWC 1 cut(s) 1266
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 7 cut(s) 847, 935, 979, 1138, 1423, 1459, 1558
BccI CCATC 4 cut(s) 565, 1232, 1462, 1520
BceAI ACGGC 2 cut(s) 70, 83
BciT130I CCWGG 5 cut(s) 332, 653, 771, 948, 1314
BcoDI GTCTC 1 cut(s) 390
BfmI CTRYAG 4 cut(s) 129, 431, 606, 1473
BfuAI ACCTGC 1 cut(s) 1300
BglI GCCNNNNNGGC 1 cut(s) 902
Bme1390I CCNGG 5 cut(s) 332, 653, 771, 948, 1314
BmgT120I GGNCC 2 cut(s) 4, 878
BmiI GGNNCC 2 cut(s) 972, 1020
BmrFI CCNGG 5 cut(s) 332, 653, 771, 948, 1314
BmsI GCATC 5 cut(s) 117, 355, 709, 914, 1381
BoxI GACNNNNGTC 1 cut(s) 1091
BpmI CTGGAG 4 cut(s) 441, 674, 866, 1338
Bpu10I CCTNAGC 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 1280
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 39, 732
BsaWI WCCGGW 2 cut(s) 694, 1441
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 5 cut(s) 644, 733, 737, 770, 1070
Bse118I RCCGGY 2 cut(s) 6, 91
Bse1I ACTGG 3 cut(s) 346, 1100, 1160
Bse21I CCTNAGG 1 cut(s) 1200
Bse3DI GCAATG 2 cut(s) 33, 468
BseBI CCWGG 5 cut(s) 332, 653, 771, 948, 1314
BseDI CCNNGG 2 cut(s) 39, 732
BseGI GGATG 1 cut(s) 1454
BseLI CCNNNNNNNGG 5 cut(s) 644, 733, 737, 770, 1070
BseMI GCAATG 2 cut(s) 33, 468
BseMII CTCAG 5 cut(s) 404, 422, 548, 653, 1214
BseNI ACTGG 3 cut(s) 346, 1100, 1160
BseRI GAGGAG 2 cut(s) 465, 681
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 7 cut(s) 847, 935, 979, 1138, 1423, 1459, 1558
BsgI GTGCAG 1 cut(s) 1557
Bsh1236I CGCG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 5 cut(s) 6, 95, 330, 880, 896
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 4 cut(s) 7, 92, 695, 1442
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 5 cut(s) 644, 733, 737, 770, 1070
BsmAI GTCTC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 5 cut(s) 6, 95, 330, 880, 896
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 222, 1253, 1266
Bsp143I GATC 2 cut(s) 150, 1481
BspANI GGCC 5 cut(s) 6, 95, 330, 880, 896
BspCNI CTCAG 5 cut(s) 405, 421, 547, 652, 1213
BspFNI CGCG 1 cut(s) 41
BspHI TCATGA 3 cut(s) 193, 1234, 1387
BspLI GGNNCC 2 cut(s) 972, 1020
BspMAI CTGCAG 1 cut(s) 1477
BspMI ACCTGC 1 cut(s) 1300
BspPI GGATC 1 cut(s) 1489
BspT104I TTCGAA 1 cut(s) 272
BsrDI GCAATG 2 cut(s) 33, 468
BsrFI RCCGGY 2 cut(s) 6, 91
BsrI ACTGG 3 cut(s) 346, 1100, 1160
BssAI RCCGGY 2 cut(s) 6, 91
BssECI CCNNGG 2 cut(s) 39, 732
BssMI GATC 2 cut(s) 150, 1481
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 732
Bst2UI CCWGG 5 cut(s) 332, 653, 771, 948, 1314
Bst4CI ACNGT 4 cut(s) 401, 610, 1093, 1327
Bst6I CTCTTC 1 cut(s) 493
BstACI GRCGYC 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 93, 553
BstDEI CTNAG 5 cut(s) 408, 413, 534, 639, 1200
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 1454
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 1226
BstKTI GATC 2 cut(s) 153, 1484
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 2 cut(s) 150, 1481
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 5 cut(s) 902, 1139, 1148, 1292, 1301
BstNI CCWGG 5 cut(s) 332, 653, 771, 948, 1314
BstNSI RCATGY 1 cut(s) 1123
BstPAI GACNNNNGTC 1 cut(s) 1091
BstSCI CCNGG 5 cut(s) 330, 651, 769, 946, 1312
BstSFI CTRYAG 4 cut(s) 129, 431, 606, 1473
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 7 cut(s) 847, 935, 979, 1138, 1423, 1459, 1558
BstX2I RGATCY 1 cut(s) 1481
BstXI CCANNNNNNTGG 1 cut(s) 871
BstYI RGATCY 1 cut(s) 1481
BstZI CGGCCG 1 cut(s) 93
Bsu36I CCTNAGG 1 cut(s) 1200
BsuRI GGCC 5 cut(s) 6, 95, 330, 880, 896
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 1454
BtsIMutI CAGTG 3 cut(s) 339, 406, 1089
BveI ACCTGC 1 cut(s) 1300
Cac8I GCNNGC 2 cut(s) 93, 553
CaiI CAGNNNCTG 2 cut(s) 770, 926
CciI TCATGA 3 cut(s) 193, 1234, 1387
CfoI GCGC 1 cut(s) 1226
Cfr10I RCCGGY 2 cut(s) 6, 91
Cfr13I GGNCC 2 cut(s) 4, 878
Cfr42I CCGCGG 1 cut(s) 42
CseI GACGC 1 cut(s) 1002
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 1 cut(s) 297
CviAII CATG 8 cut(s) 194, 748, 1096, 1120, 1156, 1235, 1388, 1540
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 5 cut(s) 408, 413, 534, 639, 1200
DpnI GATC 2 cut(s) 152, 1483
DpnII GATC 2 cut(s) 150, 1481
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 93
EagI CGGCCG 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 1264
EclXI CGGCCG 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 732
Eco24I GRGCYC 3 cut(s) 222, 1253, 1266
Eco32I GATATC 1 cut(s) 374
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 1264
Eco57I CTGAAG 3 cut(s) 731, 1024, 1194
Eco81I CCTNAGG 1 cut(s) 1200
EcoICRI GAGCTC 1 cut(s) 1264
EcoRII CCWGG 5 cut(s) 330, 651, 769, 946, 1312
EcoRV GATATC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 732
EcoT38I GRGCYC 3 cut(s) 222, 1253, 1266
ErhI CCWWGG 1 cut(s) 732
FaeI CATG 8 cut(s) 197, 751, 1099, 1123, 1159, 1238, 1391, 1543
FaqI GGGAC 1 cut(s) 620
FatI CATG 8 cut(s) 193, 747, 1095, 1119, 1155, 1234, 1387, 1539
FauI CCCGC 2 cut(s) 560, 988
FblI GTMKAC 1 cut(s) 128
FokI GGATG 1 cut(s) 1441
FriOI GRGCYC 3 cut(s) 222, 1253, 1266
GlaI GCGC 1 cut(s) 1225
GsuI CTGGAG 4 cut(s) 441, 674, 866, 1338
HaeIII GGCC 5 cut(s) 6, 95, 330, 880, 896
HapII CCGG 4 cut(s) 7, 92, 695, 1442
HgaI GACGC 1 cut(s) 1002
HhaI GCGC 1 cut(s) 1226
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 8 cut(s) 197, 751, 1099, 1123, 1159, 1238, 1391, 1543
Hin6I GCGC 1 cut(s) 1224
HinP1I GCGC 1 cut(s) 1224
HpaII CCGG 4 cut(s) 7, 92, 695, 1442
HphI GGTGA 2 cut(s) 601, 1085
Hpy166II GTNNAC 5 cut(s) 129, 261, 703, 1076, 1408
Hpy188I TCNGA 9 cut(s) 150, 381, 395, 562, 1003, 1043, 1216, 1459, 1525
Hpy8I GTNNAC 5 cut(s) 129, 261, 703, 1076, 1408
Hpy99I CGWCG 2 cut(s) 91, 1018
HpyAV CCTTC 5 cut(s) 151, 804, 869, 1169, 1472
HpyCH4III ACNGT 4 cut(s) 401, 610, 1093, 1327
HpyCH4IV ACGT 4 cut(s) 178, 257, 263, 822
HpyCH4V TGCA 7 cut(s) 293, 492, 722, 1244, 1332, 1475, 1574
HpyF10VI GCNNNNNNNGC 5 cut(s) 902, 1139, 1148, 1292, 1301
HpyF3I CTNAG 5 cut(s) 408, 413, 534, 639, 1200
HpySE526I ACGT 4 cut(s) 178, 257, 263, 822
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 8 cut(s) 197, 751, 1099, 1123, 1159, 1238, 1391, 1543
HspAI GCGC 1 cut(s) 1224
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
KspI CCGCGG 1 cut(s) 42
Kzo9I GATC 2 cut(s) 150, 1481
LmnI GCTCC 4 cut(s) 478, 976, 1024, 1444
Lsp1109I GCAGC 7 cut(s) 847, 935, 979, 1138, 1423, 1459, 1558
LweI GCATC 5 cut(s) 117, 355, 709, 914, 1381
MabI ACCWGGT 1 cut(s) 946
MaeII ACGT 4 cut(s) 178, 257, 263, 822
MaeIII GTNAC 6 cut(s) 337, 401, 657, 685, 1084, 1547
MalI GATC 2 cut(s) 152, 1483
MboI GATC 2 cut(s) 150, 1481
MboII GAAGA 6 cut(s) 220, 286, 368, 510, 724, 1568
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 1 cut(s) 1481
MhlI GDGCHC 3 cut(s) 222, 1253, 1266
MluCI AATT 9 cut(s) 18, 504, 621, 758, 933, 978, 984, 1191, 1344
MlyI GAGTC 4 cut(s) 215, 554, 665, 682
MmeI TCCRAC 1 cut(s) 373
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 4 cut(s) 344, 869, 1094, 1239
MspA1I CMGCKG 4 cut(s) 41, 412, 926, 1571
MspI CCGG 4 cut(s) 7, 92, 695, 1442
MspR9I CCNGG 5 cut(s) 332, 653, 771, 948, 1314
MunI CAATTG 1 cut(s) 933
MvaI CCWGG 5 cut(s) 332, 653, 771, 948, 1314
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 5 cut(s) 902, 1139, 1148, 1292, 1301
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 2 cut(s) 150, 1481
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 8 cut(s) 197, 751, 1099, 1123, 1159, 1238, 1391, 1543
NlaIV GGNNCC 2 cut(s) 972, 1020
NmuCI GTSAC 6 cut(s) 337, 401, 657, 685, 1084, 1547
NspI RCATGY 1 cut(s) 1123
NspV TTCGAA 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 344
PagI TCATGA 3 cut(s) 193, 1234, 1387
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PdiI GCCGGC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 770
PfoI TCCNGGA 1 cut(s) 651
PleI GAGTC 4 cut(s) 214, 553, 664, 682
PpsI GAGTC 4 cut(s) 214, 553, 664, 682
PshAI GACNNNNGTC 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 1266
Psp1406I AACGTT 1 cut(s) 822
Psp6I CCWGG 5 cut(s) 330, 651, 769, 946, 1312
PspGI CCWGG 5 cut(s) 330, 651, 769, 946, 1312
PspN4I GGNNCC 2 cut(s) 972, 1020
PspPI GGNCC 2 cut(s) 4, 878
PstI CTGCAG 1 cut(s) 1477
PstNI CAGNNNCTG 2 cut(s) 770, 926
PsuI RGATCY 1 cut(s) 1481
PvuII CAGCTG 3 cut(s) 412, 926, 1571
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
RseI CAYNNNNRTG 4 cut(s) 344, 869, 1094, 1239
SacI GAGCTC 1 cut(s) 1266
SacII CCGCGG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 173
Sau3AI GATC 2 cut(s) 150, 1481
Sau96I GGNCC 2 cut(s) 4, 878
SchI GAGTC 4 cut(s) 215, 554, 665, 682
ScrFI CCNGG 5 cut(s) 332, 653, 771, 948, 1314
SduI GDGCHC 3 cut(s) 222, 1253, 1266
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 5 cut(s) 117, 355, 709, 914, 1381
SfcI CTRYAG 4 cut(s) 129, 431, 606, 1473
Sfr303I CCGCGG 1 cut(s) 42
SfuI TTCGAA 1 cut(s) 272
SgrBI CCGCGG 1 cut(s) 42
SmiMI CAYNNNNRTG 4 cut(s) 344, 869, 1094, 1239
SmlI CTYRAG 1 cut(s) 1259
SmoI CTYRAG 1 cut(s) 1259
Sse9I AATT 9 cut(s) 18, 504, 621, 758, 933, 978, 984, 1191, 1344
SstI GAGCTC 1 cut(s) 1266
StyD4I CCNGG 5 cut(s) 330, 651, 769, 946, 1312
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 4 cut(s) 401, 610, 1093, 1327
TaiI ACGT 4 cut(s) 181, 260, 266, 825
TaqI TCGA 4 cut(s) 122, 272, 1107, 1554
TasI AATT 9 cut(s) 18, 504, 621, 758, 933, 978, 984, 1191, 1344
TatI WGTACW 1 cut(s) 296
TauI GCSGC 3 cut(s) 41, 98, 108
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 3 cut(s) 346, 406, 1096
TseFI GTSAC 6 cut(s) 337, 401, 657, 685, 1084, 1547
TseI GCWGC 7 cut(s) 860, 923, 992, 1151, 1436, 1472, 1571
Tsp45I GTSAC 6 cut(s) 337, 401, 657, 685, 1084, 1547
TspDTI ATGAA 8 cut(s) 133, 210, 261, 333, 1041, 1223, 1368, 1376
TspRI CASTG 3 cut(s) 346, 406, 1096
Van91I CCANNNNNTGG 1 cut(s) 770
XapI RAATTY 6 cut(s) 504, 621, 978, 984, 1191, 1344
XceI RCATGY 1 cut(s) 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 128
ZraI GACGTC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.