Rh5AG030300

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001372
Physical Location & Seq
Reverse (-)
2110391 .. 2111062
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG030300.1

Sequence Viewer

Length: 516 bp
ATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGAGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAAAGGACAATAAAGAATCAAAAACAGAAACATCATCTGAACCAATTGGTGAGGTACCAAGCGTAGACACAGTACGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCCGTGCATTGGTTCAGGACGAAGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCTACACCAAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCAGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTAGATCTTGAACTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTCACTGCCAGTAAACCAGTTGAGAATCTGATGGAAGAAGAGCCCCCACACCAAAGCCGAGTCAGGTGTTGGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.63

Weight (kDa)

4.3

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 156
AclI AACGTT 1 cut(s) 246
AclWI GGATC 4 cut(s) 167, 180, 320, 333
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 2 cut(s) 147, 165
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 6 cut(s) 32, 59, 185, 251, 338, 404
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 4 cut(s) 167, 180, 320, 333
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 1 cut(s) 63
ApoI RAATTY 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 152
AxyI CCTNAGG 1 cut(s) 189
BamHI GGATCC 2 cut(s) 172, 325
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 483
BccI CCATC 1 cut(s) 463
BceAI ACGGC 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 397
BmiI GGNNCC 3 cut(s) 147, 174, 327
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 4 cut(s) 91, 306, 447, 455
Bse21I CCTNAGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 352, 385
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 4 cut(s) 91, 306, 447, 455
BseRI GAGGAG 1 cut(s) 408
BshFI GGCC 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 3 cut(s) 172, 325, 397
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 147, 174, 327
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 4 cut(s) 167, 180, 320, 333
BspQI GCTCTTC 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 4 cut(s) 91, 306, 447, 455
BssAI RCCGGY 1 cut(s) 61
BssMI GATC 3 cut(s) 172, 325, 397
Bst4CI ACNGT 2 cut(s) 163, 316
Bst6I CTCTTC 1 cut(s) 471
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstBAI YACGTR 1 cut(s) 167
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 352, 385
BstKTI GATC 3 cut(s) 175, 328, 400
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 3 cut(s) 172, 325, 397
BstMWI GCNNNNNNNGC 2 cut(s) 434, 443
BstSNI TACGTA 1 cut(s) 167
BstX2I RGATCY 3 cut(s) 172, 325, 397
BstYI RGATCY 3 cut(s) 172, 325, 397
Bsu36I CCTNAGG 1 cut(s) 189
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 2 cut(s) 352, 385
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 441
BveI ACCTGC 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 61
Csp6I GTAC 2 cut(s) 146, 164
CviJI RGCY 7 cut(s) 65, 82, 194, 210, 393, 481, 495
CviKI_1 RGCY 7 cut(s) 65, 82, 194, 210, 393, 481, 495
CviQI GTAC 2 cut(s) 146, 164
DdeI CTNAG 1 cut(s) 189
DpnI GATC 3 cut(s) 174, 327, 399
DpnII GATC 3 cut(s) 172, 325, 397
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco105I TACGTA 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 483
Eco81I CCTNAGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 483
FaiI YATR 2 cut(s) 299, 319
FblI GTMKAC 1 cut(s) 156
FokI GGATG 2 cut(s) 359, 392
FriOI GRGCYC 1 cut(s) 483
FspBI CTAG 1 cut(s) 395
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 62
HinfI GANTC 3 cut(s) 107, 463, 498
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 3 cut(s) 157, 244, 452
Hpy188I TCNGA 5 cut(s) 13, 130, 205, 358, 468
Hpy188III TCNNGA 3 cut(s) 224, 377, 401
Hpy8I GTNNAC 3 cut(s) 157, 244, 452
HpyCH4III ACNGT 2 cut(s) 163, 316
HpyCH4IV ACGT 2 cut(s) 166, 246
HpyCH4V TGCA 4 cut(s) 215, 368, 428, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 443
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 2 cut(s) 166, 246
KpnI GGTACC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 172, 325, 397
LguI GCTCTTC 1 cut(s) 471
MaeI CTAG 1 cut(s) 395
MaeII ACGT 2 cut(s) 166, 246
MalI GATC 3 cut(s) 174, 327, 399
MboI GATC 3 cut(s) 172, 325, 397
MboII GAAGA 2 cut(s) 485, 488
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 3 cut(s) 172, 325, 397
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 3 cut(s) 135, 180, 333
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 7 cut(s) 43, 136, 186, 198, 337, 339, 429
MspI CCGG 1 cut(s) 62
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 434, 443
NdeII GATC 3 cut(s) 172, 325, 397
NlaIV GGNNCC 3 cut(s) 147, 174, 327
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 107, 463
PflFI GACNNNGTC 1 cut(s) 7
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 167
Psp1406I AACGTT 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 147, 174, 327
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 3 cut(s) 172, 325, 397
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 147, 165
RsaNI GTAC 2 cut(s) 146, 164
SapI GCTCTTC 1 cut(s) 471
Sau3AI GATC 3 cut(s) 172, 325, 397
SchI GAGTC 1 cut(s) 507
SduI GDGCHC 1 cut(s) 483
SetI ASST 7 cut(s) 147, 169, 190, 249, 283, 436, 506
SnaBI TACGTA 1 cut(s) 167
Sse9I AATT 3 cut(s) 135, 180, 333
SspMI CTAG 1 cut(s) 395
TaaI ACNGT 2 cut(s) 163, 316
TaiI ACGT 2 cut(s) 169, 249
TasI AATT 3 cut(s) 135, 180, 333
TfiI GAWTC 2 cut(s) 107, 463
TscAI CASTG 1 cut(s) 448
TspDTI ATGAA 1 cut(s) 36
TspRI CASTG 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 7
XapI RAATTY 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.