MD05G1343900.v1.1

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001372
Physical Location & Seq
Reverse (-)
46367281 .. 46371851
4571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1343900.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGAATCAACAGAGTCAAGGAACGGGCTCTTTATCTTCACCAGACCCCCCTATGAAGCGAAAACGTGGACGTCCACGTAAGGATGAGAACTTGGCTCCGGCAGAGAGTCCAACTGTGATGCCAGGAGCTGACAGTTTGAAGAAAACCAAACAGAGTGCAAGTGCAAGTGATGATGTTGAGAATGAAATGGTAGGCCAGGTAGTCACTGGCGTGATTGAAGGGGCATTTGATGCTGGATATCTTCTGAATGTTAGGGTCGGAGACACTGACACTCAGCTGAGGGGTGTTGTATTCCTACAGGAGAAAATCACTCCCATTACACCAGCAAATGACATTGCTCCGCATATTAGGATGTGCAAAAGAAGAGAAATTCCCCTACCGCCTCTCAACCCACAATCTCAGCGTCATGAGTCTGCCCGCCCATCGGAACAAAGTAATAGGCAACCTGTTGATGTGAAACCCCAAATACCTACAGTTCCTAACCAAACTCAATCCTCTGTCCCTCAGTCTGGAATCTCGGAGTCACTTGAAAACAAATCTTTCCTTTTTATGATTCCTCCAGTTGTTAATTTGCCGAAGAATGATGCAGGCCTGCCCTTGGGAAGAAAAGTCATGTCACAACAATTTCCGGAACCAAGAACTGATAGCCAATCTGCCCCTGCGATGCCAAAGCCAGAGCATAATAATGTTGTTGAACAGGAGAAAGTGTTGGAGGAAGTTGGAGCTGCCACAGTAATGGAAGGGCCAAATGGTTTCGAGGCCAATAAAGCATCAAAAACAGAAACAACAGCTGAACCAATTGCTGACGTACCCGGCGTTGAACCTGTGTGTAAGGCTCCTGAAATTCAAACTCAGGCTGTGGGTTCTGAAAAGAGTACATCATTTGCTGATGAAGTAAACAGCTTTGGACTTGAACTCAACCAAACGCCTGTATTTGCTGAACCCTCAGCAATGACTGTCATGCCAGTTGGTGAACTCAACCAAACACCTGCATCTGCTGAACCCGGAGCAATTTCTGTCATGCCAGTCGTTGAAACTGAAGTTAAGGATCATCAAATTCAAGCTCAGGCTGTGGATTCTGAAAATGAAAAGAGCGCATTGGGTCATGATGGTGCAAAGAGCCCAAATCTCGAGCTCAACCAAACTCCTATATTTGCCGAACCTGCCTCTGTCGCTGTCATAACAGGCATTGAAACCCTATGTAAGGATTCTCAAATCCAACCTCCAGATTTCGGTCCTGAAAATAGTTCATTGATTCATGATGCGGTCAAGAGCCTGGACGTAGAACTCAGCCAAACTCCTTTTGCTGCAACTGTTACTATGCCATCCGTATCAAAGATTGCTGCGGAAGGATCGATTCCTCTCGGAACTGCCGAACCTCAAATCCCTCCTTCTGATGGCATTAGCAGCGTGGAGTGTGACGTCGAAGATGTCATTCTACCTGCACAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

485

Amino Acids

51.46

Weight (kDa)

4.62

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 997
AatII GACGTC 2 cut(s) 73, 1425
Acc36I ACCTGC 3 cut(s) 997, 1171, 1450
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 5 cut(s) 341, 380, 418, 1265, 1346
AclWI GGATC 2 cut(s) 1056, 1360
AcsI RAATTY 3 cut(s) 369, 843, 1056
AcuI CTGAAG 1 cut(s) 1059
AcyI GRCGYC 2 cut(s) 70, 1422
AfaI GTAC 2 cut(s) 810, 877
AfiI CCNNNNNNNGG 6 cut(s) 424, 509, 598, 1204, 1232, 1397
AjnI CCWGG 3 cut(s) 121, 195, 1275
AleI CACNNNNGTG 1 cut(s) 209
AloI GAACNNNNNNTCC 2 cut(s) 1368, 1400
AluBI AGCT 7 cut(s) 128, 277, 725, 791, 903, 1064, 1135
AluI AGCT 7 cut(s) 128, 277, 725, 791, 903, 1064, 1135
Alw21I GWGCWC 1 cut(s) 1137
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 1056, 1360
AlwNI CAGNNNCTG 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 1130
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 4 cut(s) 193, 589, 743, 759
ApeKI GCWGC 4 cut(s) 725, 1307, 1343, 1407
ApoI RAATTY 3 cut(s) 369, 843, 1056
AspLEI GCGC 1 cut(s) 1097
AspS9I GGNCC 2 cut(s) 743, 1235
AsuC2I CCSGG 2 cut(s) 813, 1005
AsuHPI GGTGA 2 cut(s) 30, 983
AvaI CYCGRG 1 cut(s) 1130
AvaII GGWCC 1 cut(s) 1235
BanII GRGCYC 3 cut(s) 29, 1124, 1137
Bbv12I GWGCWC 1 cut(s) 1137
BbvCI CCTCAGC 2 cut(s) 278, 946
BbvI GCAGC 4 cut(s) 712, 1294, 1330, 1419
BccI CCATC 4 cut(s) 430, 1103, 1333, 1391
BciT130I CCWGG 3 cut(s) 123, 197, 1277
BcnI CCSGG 2 cut(s) 813, 1005
BcoDI GTCTC 1 cut(s) 255
BfmI CTRYAG 2 cut(s) 296, 471
BfuAI ACCTGC 3 cut(s) 997, 1171, 1450
BisI GCNGC 4 cut(s) 726, 1308, 1344, 1408
BlsI GCNGC 4 cut(s) 727, 1309, 1345, 1409
Bme1390I CCNGG 5 cut(s) 123, 197, 813, 1005, 1277
Bme18I GGWCC 1 cut(s) 1235
BmeT110I CYCGRG 1 cut(s) 1130
BmgT120I GGNCC 2 cut(s) 743, 1235
BmiI GGNNCC 3 cut(s) 96, 633, 837
BmrFI CCNGG 5 cut(s) 123, 197, 813, 1005, 1277
BmsI GCATC 7 cut(s) 108, 220, 574, 654, 779, 1001, 1252
BpmI CTGGAG 2 cut(s) 543, 1209
Bpu10I CCTNAGC 3 cut(s) 278, 946, 1065
BpuMI CCSGG 2 cut(s) 813, 1005
Bsa29I ATCGAT 1 cut(s) 1355
BsaAI YACGTR 1 cut(s) 77
BsaHI GRCGYC 2 cut(s) 70, 1422
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 628
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 6 cut(s) 424, 509, 598, 1204, 1232, 1397
Bse1I ACTGG 4 cut(s) 211, 560, 965, 1025
Bse3DI GCAATG 2 cut(s) 333, 957
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 3 cut(s) 123, 197, 1277
BseCI ATCGAT 1 cut(s) 1355
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 3 cut(s) 88, 357, 1325
BseLI CCNNNNNNNGG 6 cut(s) 424, 509, 598, 1204, 1232, 1397
BseMI GCAATG 2 cut(s) 333, 957
BseMII CTCAG 8 cut(s) 269, 287, 413, 518, 866, 960, 1079, 1303
BseNI ACTGG 4 cut(s) 211, 560, 965, 1025
BseXI GCAGC 4 cut(s) 712, 1294, 1330, 1419
BsgI GTGCAG 1 cut(s) 1428
BshFI GGCC 4 cut(s) 195, 591, 745, 761
BshVI ATCGAT 1 cut(s) 1355
BsiHKAI GWGCWC 1 cut(s) 1137
BsiHKCI CYCGRG 1 cut(s) 1130
BsiSI CCGG 4 cut(s) 98, 629, 813, 1005
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 6 cut(s) 424, 509, 598, 1204, 1232, 1397
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 4 cut(s) 195, 591, 745, 761
BsoBI CYCGRG 1 cut(s) 1130
Bsp1286I GDGCHC 3 cut(s) 29, 1124, 1137
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 2 cut(s) 1048, 1352
BspACI CCGC 5 cut(s) 341, 380, 418, 1265, 1346
BspANI GGCC 4 cut(s) 195, 591, 745, 761
BspCNI CTCAG 8 cut(s) 270, 286, 412, 517, 865, 959, 1078, 1302
BspDI ATCGAT 1 cut(s) 1355
BspEI TCCGGA 1 cut(s) 628
BspHI TCATGA 3 cut(s) 406, 1105, 1258
BspLI GGNNCC 3 cut(s) 96, 633, 837
BspMI ACCTGC 3 cut(s) 997, 1171, 1450
BspPI GGATC 2 cut(s) 1056, 1360
BsrDI GCAATG 2 cut(s) 333, 957
BsrI ACTGG 4 cut(s) 211, 560, 965, 1025
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 2 cut(s) 1048, 1352
BssNI GRCGYC 2 cut(s) 70, 1422
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 3 cut(s) 123, 197, 1277
Bst4CI ACNGT 6 cut(s) 115, 134, 475, 733, 958, 1315
Bst6I CTCTTC 1 cut(s) 358
BstACI GRCGYC 2 cut(s) 70, 1422
BstAPI GCANNNNNTGC 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 418, 589, 593
BstDEI CTNAG 8 cut(s) 273, 278, 399, 504, 852, 946, 1065, 1289
BstENI CCTNNNNNAGG 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 88, 357, 1325
BstHHI GCGC 1 cut(s) 1097
BstKTI GATC 2 cut(s) 1051, 1355
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 2 cut(s) 1048, 1352
BstMWI GCNNNNNNNGC 5 cut(s) 230, 767, 1163, 1172, 1407
BstNI CCWGG 3 cut(s) 123, 197, 1277
BstSCI CCNGG 5 cut(s) 121, 195, 811, 1003, 1275
BstSFI CTRYAG 2 cut(s) 296, 471
BstV1I GCAGC 4 cut(s) 712, 1294, 1330, 1419
BstXI CCANNNNNNTGG 1 cut(s) 736
Bsu15I ATCGAT 1 cut(s) 1355
BsuRI GGCC 4 cut(s) 195, 591, 745, 761
BsuTUI ATCGAT 1 cut(s) 1355
BtgZI GCGATG 1 cut(s) 677
BtsCI GGATG 3 cut(s) 88, 357, 1325
BtsIMutI CAGTG 2 cut(s) 204, 264
BveI ACCTGC 3 cut(s) 997, 1171, 1450
Cac8I GCNNGC 3 cut(s) 418, 589, 593
CaiI CAGNNNCTG 1 cut(s) 128
CciI TCATGA 3 cut(s) 406, 1105, 1258
CfoI GCGC 1 cut(s) 1097
Cfr13I GGNCC 2 cut(s) 743, 1235
ClaI ATCGAT 1 cut(s) 1355
CseI GACGC 1 cut(s) 392
Csp6I GTAC 2 cut(s) 809, 876
CviAII CATG 6 cut(s) 407, 613, 961, 1021, 1106, 1259
CviQI GTAC 2 cut(s) 809, 876
DdeI CTNAG 8 cut(s) 273, 278, 399, 504, 852, 946, 1065, 1289
DpnI GATC 2 cut(s) 1050, 1354
DpnII GATC 2 cut(s) 1048, 1352
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 1135
Eco130I CCWWGG 1 cut(s) 597
Eco147I AGGCCT 1 cut(s) 591
Eco24I GRGCYC 3 cut(s) 29, 1124, 1137
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 1235
Eco53kI GAGCTC 1 cut(s) 1135
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 1130
EcoICRI GAGCTC 1 cut(s) 1135
EcoNI CCTNNNNNAGG 1 cut(s) 1202
EcoRII CCWGG 3 cut(s) 121, 195, 1275
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 3 cut(s) 29, 1124, 1137
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 6 cut(s) 410, 616, 964, 1024, 1109, 1262
FaqI GGGAC 1 cut(s) 485
FatI CATG 6 cut(s) 406, 612, 960, 1020, 1105, 1258
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 4 cut(s) 726, 1308, 1344, 1408
FokI GGATG 3 cut(s) 95, 364, 1312
FriOI GRGCYC 3 cut(s) 29, 1124, 1137
Fsp4HI GCNGC 4 cut(s) 726, 1308, 1344, 1408
GlaI GCGC 1 cut(s) 1096
GluI GCNGC 4 cut(s) 726, 1308, 1344, 1408
GsuI CTGGAG 2 cut(s) 543, 1209
HaeIII GGCC 4 cut(s) 195, 591, 745, 761
HapII CCGG 4 cut(s) 98, 629, 813, 1005
HgaI GACGC 1 cut(s) 392
HhaI GCGC 1 cut(s) 1097
Hin1I GRCGYC 2 cut(s) 70, 1422
Hin1II CATG 6 cut(s) 410, 616, 964, 1024, 1109, 1262
Hin6I GCGC 1 cut(s) 1095
HinP1I GCGC 1 cut(s) 1095
HpaII CCGG 4 cut(s) 98, 629, 813, 1005
HphI GGTGA 2 cut(s) 30, 983
Hpy166II GTNNAC 4 cut(s) 68, 74, 898, 974
Hpy188I TCNGA 8 cut(s) 246, 260, 427, 520, 868, 1081, 1367, 1396
Hpy8I GTNNAC 4 cut(s) 68, 74, 898, 974
Hpy99I CGWCG 1 cut(s) 1427
HpyAV CCTTC 4 cut(s) 212, 734, 1343, 1401
HpyCH4III ACNGT 6 cut(s) 115, 134, 475, 733, 958, 1315
HpyCH4IV ACGT 6 cut(s) 64, 70, 76, 807, 1281, 1422
HpyCH4V TGCA 8 cut(s) 158, 164, 357, 587, 992, 1115, 1310, 1445
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 767, 1163, 1172, 1407
HpyF3I CTNAG 8 cut(s) 273, 278, 399, 504, 852, 946, 1065, 1289
HpySE526I ACGT 6 cut(s) 64, 70, 76, 807, 1281, 1422
Hsp92I GRCGYC 2 cut(s) 70, 1422
Hsp92II CATG 6 cut(s) 410, 616, 964, 1024, 1109, 1262
HspAI GCGC 1 cut(s) 1095
Kpn2I TCCGGA 1 cut(s) 628
Kzo9I GATC 2 cut(s) 1048, 1352
LmnI GCTCC 6 cut(s) 100, 125, 343, 722, 841, 1007
Lsp1109I GCAGC 4 cut(s) 712, 1294, 1330, 1419
LweI GCATC 7 cut(s) 108, 220, 574, 654, 779, 1001, 1252
MaeII ACGT 6 cut(s) 64, 70, 76, 807, 1281, 1422
MaeIII GTNAC 5 cut(s) 202, 522, 615, 1315, 1418
MalI GATC 2 cut(s) 1050, 1354
MboI GATC 2 cut(s) 1048, 1352
MboII GAAGA 7 cut(s) 27, 151, 233, 375, 589, 615, 1439
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 3 cut(s) 29, 1124, 1137
MluCI AATT 7 cut(s) 369, 568, 623, 798, 843, 1011, 1056
MlyI GAGTC 4 cut(s) 22, 115, 419, 530
MmeI TCCRAC 5 cut(s) 134, 238, 690, 700, 1243
MroI TCCGGA 1 cut(s) 628
MseI TTAA 2 cut(s) 567, 1044
MslI CAYNNNNRTG 4 cut(s) 209, 684, 734, 1110
MspA1I CMGCKG 2 cut(s) 277, 791
MspI CCGG 4 cut(s) 98, 629, 813, 1005
MspR9I CCNGG 5 cut(s) 123, 197, 813, 1005, 1277
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 3 cut(s) 123, 197, 1277
MwoI GCNNNNNNNGC 5 cut(s) 230, 767, 1163, 1172, 1407
NciI CCSGG 2 cut(s) 813, 1005
NdeII GATC 2 cut(s) 1048, 1352
NlaIII CATG 6 cut(s) 410, 616, 964, 1024, 1109, 1262
NlaIV GGNNCC 3 cut(s) 96, 633, 837
NmuCI GTSAC 4 cut(s) 202, 522, 615, 1418
OliI CACNNNNGTG 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 1130
PagI TCATGA 3 cut(s) 406, 1105, 1258
PaqCI CACCTGC 1 cut(s) 997
PceI AGGCCT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 2 cut(s) 813, 1371
PfeI GAWTC 7 cut(s) 4, 513, 553, 1076, 1208, 1255, 1357
PkrI GCNGC 4 cut(s) 727, 1309, 1345, 1409
PleI GAGTC 4 cut(s) 21, 114, 418, 529
PpsI GAGTC 4 cut(s) 21, 114, 418, 529
Ppu21I YACGTR 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 1137
Psp6I CCWGG 3 cut(s) 121, 195, 1275
PspGI CCWGG 3 cut(s) 121, 195, 1275
PspN4I GGNNCC 3 cut(s) 96, 633, 837
PspPI GGNCC 2 cut(s) 743, 1235
PstNI CAGNNNCTG 1 cut(s) 128
PvuII CAGCTG 2 cut(s) 277, 791
RsaI GTAC 2 cut(s) 810, 877
RsaNI GTAC 2 cut(s) 809, 876
RseI CAYNNNNRTG 4 cut(s) 209, 684, 734, 1110
SacI GAGCTC 1 cut(s) 1137
SaqAI TTAA 2 cut(s) 567, 1044
SatI GCNGC 4 cut(s) 726, 1308, 1344, 1408
Sau3AI GATC 2 cut(s) 1048, 1352
Sau96I GGNCC 2 cut(s) 743, 1235
SchI GAGTC 4 cut(s) 22, 115, 419, 530
ScrFI CCNGG 5 cut(s) 123, 197, 813, 1005, 1277
SduI GDGCHC 3 cut(s) 29, 1124, 1137
SfaNI GCATC 7 cut(s) 108, 220, 574, 654, 779, 1001, 1252
SfcI CTRYAG 2 cut(s) 296, 471
Sfr274I CTCGAG 1 cut(s) 1130
SinI GGWCC 1 cut(s) 1235
SlaI CTCGAG 1 cut(s) 1130
SmiMI CAYNNNNRTG 4 cut(s) 209, 684, 734, 1110
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
Sse9I AATT 7 cut(s) 369, 568, 623, 798, 843, 1011, 1056
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 5 cut(s) 341, 380, 418, 1265, 1346
SstI GAGCTC 1 cut(s) 1137
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 5 cut(s) 121, 195, 811, 1003, 1275
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 6 cut(s) 115, 134, 475, 733, 958, 1315
TaiI ACGT 6 cut(s) 67, 73, 79, 810, 1284, 1425
TaqI TCGA 4 cut(s) 756, 1131, 1355, 1425
TaqII GACCGA 1 cut(s) 1223
TasI AATT 7 cut(s) 369, 568, 623, 798, 843, 1011, 1056
TatI WGTACW 1 cut(s) 875
TfiI GAWTC 7 cut(s) 4, 513, 553, 1076, 1208, 1255, 1357
Tru1I TTAA 2 cut(s) 567, 1044
Tru9I TTAA 2 cut(s) 567, 1044
TscAI CASTG 2 cut(s) 211, 271
TseFI GTSAC 4 cut(s) 202, 522, 615, 1418
TseI GCWGC 4 cut(s) 725, 1307, 1343, 1407
Tsp45I GTSAC 4 cut(s) 202, 522, 615, 1418
TspDTI ATGAA 7 cut(s) 17, 68, 198, 906, 1101, 1239, 1247
TspGWI ACGGA 1 cut(s) 1318
TspRI CASTG 2 cut(s) 211, 271
VpaK11BI GGWCC 1 cut(s) 1235
XagI CCTNNNNNAGG 1 cut(s) 1202
XapI RAATTY 3 cut(s) 369, 843, 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 1130
ZraI GACGTC 2 cut(s) 71, 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.