pycom05g31330

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001372
Physical Location & Seq
Reverse (-)
31098843 .. 31100522
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31330.2

Sequence Viewer

Length: 1473 bp
ATGAGGAATAGCTTTGTCTCTCTGGTTCAGGACTTAGGGTTACCATTGGTATTCATTTCACTTTCTTTTCCTTCCATTTGTAGTCGAATCAGTCAAGGAACGGGCTCTTTATCTTCGCCAGACCCCCCTATGAAGCGAAAACGTGGACGTCCACGTAAGGATGAGAACTTAGCTCCGGCAGAGAGTCCAACTGTGATGCCAGGAGCTGACAGTTTGAAGAAAACCAAACAGAGTGCAAGTACAAGTGATGATGTTGAGAATGAAATGGTAGGCCAGGTAGTCACTGGCGTGATTGAAGGGGCATTTGATGCTGGATATCTTCTGAATGTTAGGGTCGGAGACACTGACACTCAGCTGAGGGGTGTTGTATTCCTACAGGAGAAAATCACTCCCATTACACCAGCAAATGACATTGCTCCGCATATTAGGATGTGCAAAAGAAGAGAAATTCCCCTACCGCCTCTCAACCCACAATCTCAGCGTTATGAGTCTACCCGCCCATCAGACCAAAGTAATAGGCAACCTGTTGATGTGAAACCCCAAATACCTACAGTTCCTAACCAAACTCAATCCTCTGTCCTTAAGTCTGGAATCCCGGAGTCACTTGAAAACAAATCTTTCCTTGTGATTCCTCCGGTTGTTAATTTGCCGAAGAATGATGCGGGCCTACCCTTGAGAAGAAAAGTCATGTCACAACAATTTCCGGAACCTGGAACTGATAGCCAATCTGCCCCTGCGATGCCAAAGCCAGAGCATAATAATGTTGTTGAACAGGAGAAAGTGTTGGAGGAAGTTGAATCTGCCACAGTAATGGAAGGGCCAAATGGTGTTGAGGCCAATAAAGCATCAAAAACAGAAACAACAGCTGAACCAGTTGCTGACATGGCAGGCGTTGAACCTGTGTGTAAGGCTCCTGAAATTCAAACTCAGGCTGTGGGTTCTGAAAAGAGTACATCATTTGCTGATGAAGTCAAGAGCTTTGGACTTGAACGCAACCAGACGCCTGTATTTGCCGAACCCTCAGCAATGACTGTCATGCCAGTTGTCGTTGAAACTGAAGTTAAGGATCATCAAATTCAAGCTGAGGCCGTGGATTCTGAAAATGAAAAGAGCGCATTGGGTCATGATGGTGCAAAGAGCCCAAATCTCGAGCTCAACCAAACTTCTATATTTGCCGAACCTGCCTCTGTCGCTGTCATAACAGGCATTGAAACCCTATGTAAGGATTCTCAAATCCAACCTCCAGAGTTCGGTCCTGAAAATAGTTCATTGGTTCATGATGCGGTCAAGAGCCTGGACTTCGAACTCAACCAAACTCCTTTTGCTGCACCTGTTAGTATGCCATCCGTATCAAACATTGCTGCAGAAGGATCTATTCCTCTAGGAACTGCCGAACTTCAAATCCCTACTTCTGATGGCATTAGCAGCGTGGAGTGTGACGTCGAAGATGTCATTCCACCTGCGCAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

491

Amino Acids

52.48

Weight (kDa)

4.68

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1468
AatII GACGTC 2 cut(s) 151, 1443
Acc16I TGCGCA 1 cut(s) 1464
Acc36I ACCTGC 2 cut(s) 1189, 1468
AccI GTMKAC 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 703
AciI CCGC 5 cut(s) 419, 458, 496, 662, 1283
AclWI GGATC 2 cut(s) 1074, 1378
AcsI RAATTY 3 cut(s) 447, 918, 1074
AcuI CTGAAG 1 cut(s) 1077
AcyI GRCGYC 3 cut(s) 148, 1001, 1440
AfaI GTAC 2 cut(s) 241, 952
AfiI CCNNNNNNNGG 3 cut(s) 710, 1222, 1250
AflII CTTAAG 1 cut(s) 581
AjnI CCWGG 4 cut(s) 199, 273, 709, 1293
AleI CACNNNNGTG 1 cut(s) 287
AloI GAACNNNNNNTCC 2 cut(s) 1386, 1418
AluBI AGCT 8 cut(s) 12, 173, 206, 355, 866, 978, 1082, 1153
AluI AGCT 8 cut(s) 12, 173, 206, 355, 866, 978, 1082, 1153
Alw21I GWGCWC 1 cut(s) 1155
Alw26I GTCTC 2 cut(s) 22, 333
AlwI GGATC 2 cut(s) 1074, 1378
AlwNI CAGNNNCTG 2 cut(s) 206, 878
Ama87I CYCGRG 1 cut(s) 1148
Aor13HI TCCGGA 1 cut(s) 703
AoxI GGCC 5 cut(s) 271, 664, 818, 834, 1086
ApeKI GCWGC 3 cut(s) 1325, 1361, 1425
ApoI RAATTY 3 cut(s) 447, 918, 1074
AspLEI GCGC 2 cut(s) 1115, 1465
AspS9I GGNCC 3 cut(s) 664, 818, 1253
AsuC2I CCSGG 1 cut(s) 596
AsuII TTCGAA 1 cut(s) 1302
AvaI CYCGRG 1 cut(s) 1148
AvaII GGWCC 1 cut(s) 1253
BanII GRGCYC 3 cut(s) 107, 1142, 1155
Bbv12I GWGCWC 1 cut(s) 1155
BbvCI CCTCAGC 3 cut(s) 356, 1021, 1083
BbvI GCAGC 3 cut(s) 1312, 1348, 1437
BccI CCATC 4 cut(s) 508, 1121, 1351, 1409
BceAI ACGGC 1 cut(s) 1073
BciT130I CCWGG 4 cut(s) 201, 275, 711, 1295
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 2 cut(s) 22, 333
BfaI CTAG 1 cut(s) 1382
BfmI CTRYAG 3 cut(s) 374, 549, 1362
BfrI CTTAAG 1 cut(s) 581
BfuAI ACCTGC 2 cut(s) 1189, 1468
BisI GCNGC 3 cut(s) 1326, 1362, 1426
BlsI GCNGC 3 cut(s) 1327, 1363, 1427
Bme1390I CCNGG 5 cut(s) 201, 275, 596, 711, 1295
Bme18I GGWCC 1 cut(s) 1253
BmeT110I CYCGRG 1 cut(s) 1148
BmgT120I GGNCC 3 cut(s) 664, 818, 1253
BmiI GGNNCC 2 cut(s) 708, 912
BmrFI CCNGG 5 cut(s) 201, 275, 596, 711, 1295
BmsI GCATC 6 cut(s) 186, 298, 649, 729, 854, 1270
BpmI CTGGAG 1 cut(s) 1227
Bpu10I CCTNAGC 3 cut(s) 356, 1021, 1083
Bpu14I TTCGAA 1 cut(s) 1302
BpuEI CTTGAG 1 cut(s) 694
BpuMI CCSGG 1 cut(s) 596
BsaAI YACGTR 1 cut(s) 155
BsaHI GRCGYC 3 cut(s) 148, 1001, 1440
BsaJI CCNNGG 1 cut(s) 1089
BsaWI WCCGGW 2 cut(s) 634, 703
BsaXI ACNNNNNCTCC 2 cut(s) 767, 797
Bsc4I CCNNNNNNNGG 3 cut(s) 710, 1222, 1250
Bse1I ACTGG 3 cut(s) 289, 872, 1040
Bse3DI GCAATG 3 cut(s) 411, 1032, 1356
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 4 cut(s) 201, 275, 711, 1295
BseDI CCNNGG 1 cut(s) 1089
BseGI GGATG 3 cut(s) 166, 435, 1343
BseLI CCNNNNNNNGG 3 cut(s) 710, 1222, 1250
BseMI GCAATG 3 cut(s) 411, 1032, 1356
BseMII CTCAG 6 cut(s) 347, 365, 491, 941, 1035, 1074
BseNI ACTGG 3 cut(s) 289, 872, 1040
BseXI GCAGC 3 cut(s) 1312, 1348, 1437
BsgI GTGCAG 1 cut(s) 1311
BshFI GGCC 5 cut(s) 273, 666, 820, 836, 1088
BsiHKAI GWGCWC 1 cut(s) 1155
BsiHKCI CYCGRG 1 cut(s) 1148
BsiSI CCGG 4 cut(s) 176, 596, 635, 704
BslI CCNNNNNNNGG 3 cut(s) 710, 1222, 1250
BsmAI GTCTC 2 cut(s) 22, 333
BsnI GGCC 5 cut(s) 273, 666, 820, 836, 1088
BsoBI CYCGRG 1 cut(s) 1148
Bsp119I TTCGAA 1 cut(s) 1302
Bsp1286I GDGCHC 3 cut(s) 107, 1142, 1155
Bsp13I TCCGGA 1 cut(s) 703
Bsp143I GATC 2 cut(s) 1066, 1370
BspACI CCGC 5 cut(s) 419, 458, 496, 662, 1283
BspANI GGCC 5 cut(s) 273, 666, 820, 836, 1088
BspCNI CTCAG 6 cut(s) 348, 364, 490, 940, 1034, 1075
BspEI TCCGGA 1 cut(s) 703
BspHI TCATGA 2 cut(s) 1123, 1276
BspLI GGNNCC 2 cut(s) 708, 912
BspMAI CTGCAG 1 cut(s) 1366
BspMI ACCTGC 2 cut(s) 1189, 1468
BspPI GGATC 2 cut(s) 1074, 1378
BspT104I TTCGAA 1 cut(s) 1302
BspTI CTTAAG 1 cut(s) 581
BsrDI GCAATG 3 cut(s) 411, 1032, 1356
BsrI ACTGG 3 cut(s) 289, 872, 1040
BssECI CCNNGG 1 cut(s) 1089
BssMI GATC 2 cut(s) 1066, 1370
BssNI GRCGYC 3 cut(s) 148, 1001, 1440
Bst2UI CCWGG 4 cut(s) 201, 275, 711, 1295
Bst4CI ACNGT 5 cut(s) 193, 212, 553, 808, 1033
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 3 cut(s) 148, 1001, 1440
BstAFI CTTAAG 1 cut(s) 581
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 1302
BstC8I GCNNGC 2 cut(s) 664, 889
BstDEI CTNAG 8 cut(s) 34, 169, 351, 356, 477, 927, 1021, 1083
BstDSI CCRYGG 1 cut(s) 1089
BstEII GGTNACC 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 1220
BstF5I GGATG 3 cut(s) 166, 435, 1343
BstHHI GCGC 2 cut(s) 1115, 1465
BstKTI GATC 2 cut(s) 1069, 1373
BstMAI GTCTC 2 cut(s) 22, 333
BstMBI GATC 2 cut(s) 1066, 1370
BstMWI GCNNNNNNNGC 6 cut(s) 308, 842, 884, 1181, 1190, 1425
BstNI CCWGG 4 cut(s) 201, 275, 711, 1295
BstPI GGTNACC 1 cut(s) 39
BstSCI CCNGG 5 cut(s) 199, 273, 594, 709, 1293
BstSFI CTRYAG 3 cut(s) 374, 549, 1362
BstV1I GCAGC 3 cut(s) 1312, 1348, 1437
BstX2I RGATCY 1 cut(s) 1370
BstXI CCANNNNNNTGG 1 cut(s) 811
BstYI RGATCY 1 cut(s) 1370
BsuRI GGCC 5 cut(s) 273, 666, 820, 836, 1088
BtgI CCRYGG 1 cut(s) 1089
BtgZI GCGATG 1 cut(s) 752
BtsCI GGATG 3 cut(s) 166, 435, 1343
BtsIMutI CAGTG 2 cut(s) 282, 342
BveI ACCTGC 2 cut(s) 1189, 1468
Cac8I GCNNGC 2 cut(s) 664, 889
CaiI CAGNNNCTG 2 cut(s) 206, 878
CciI TCATGA 2 cut(s) 1123, 1276
CfoI GCGC 2 cut(s) 1115, 1465
Cfr13I GGNCC 3 cut(s) 664, 818, 1253
CseI GACGC 1 cut(s) 1009
Csp6I GTAC 2 cut(s) 240, 951
CviAII CATG 5 cut(s) 688, 883, 1036, 1124, 1277
CviQI GTAC 2 cut(s) 240, 951
DdeI CTNAG 8 cut(s) 34, 169, 351, 356, 477, 927, 1021, 1083
DpnI GATC 2 cut(s) 1068, 1372
DpnII GATC 2 cut(s) 1066, 1370
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 1153
Eco24I GRGCYC 3 cut(s) 107, 1142, 1155
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 1253
Eco53kI GAGCTC 1 cut(s) 1153
Eco57I CTGAAG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 1148
Eco91I GGTNACC 1 cut(s) 39
EcoICRI GAGCTC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 39
EcoRII CCWGG 4 cut(s) 199, 273, 709, 1293
EcoRV GATATC 1 cut(s) 317
EcoT38I GRGCYC 3 cut(s) 107, 1142, 1155
FaeI CATG 5 cut(s) 691, 886, 1039, 1127, 1280
FatI CATG 5 cut(s) 687, 882, 1035, 1123, 1276
FauI CCCGC 2 cut(s) 503, 655
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 3 cut(s) 1326, 1362, 1426
FokI GGATG 3 cut(s) 173, 442, 1330
FriOI GRGCYC 3 cut(s) 107, 1142, 1155
Fsp4HI GCNGC 3 cut(s) 1326, 1362, 1426
FspBI CTAG 1 cut(s) 1382
FspI TGCGCA 1 cut(s) 1464
GlaI GCGC 2 cut(s) 1114, 1464
GluI GCNGC 3 cut(s) 1326, 1362, 1426
GsuI CTGGAG 1 cut(s) 1227
HaeIII GGCC 5 cut(s) 273, 666, 820, 836, 1088
HapII CCGG 4 cut(s) 176, 596, 635, 704
HgaI GACGC 1 cut(s) 1009
HhaI GCGC 2 cut(s) 1115, 1465
Hin1I GRCGYC 3 cut(s) 148, 1001, 1440
Hin1II CATG 5 cut(s) 691, 886, 1039, 1127, 1280
Hin6I GCGC 2 cut(s) 1113, 1463
HinP1I GCGC 2 cut(s) 1113, 1463
HinfI GANTC 9 cut(s) 87, 184, 488, 591, 599, 628, 797, 1094, 1226
HpaII CCGG 4 cut(s) 176, 596, 635, 704
Hpy166II GTNNAC 3 cut(s) 146, 152, 492
Hpy188I TCNGA 6 cut(s) 324, 338, 505, 943, 1099, 1414
Hpy8I GTNNAC 3 cut(s) 146, 152, 492
Hpy99I CGWCG 1 cut(s) 1445
HpyAV CCTTC 4 cut(s) 81, 290, 809, 1361
HpyCH4III ACNGT 5 cut(s) 193, 212, 553, 808, 1033
HpyCH4IV ACGT 4 cut(s) 142, 148, 154, 1440
HpyCH4V TGCA 5 cut(s) 236, 435, 1133, 1328, 1364
HpyF10VI GCNNNNNNNGC 6 cut(s) 308, 842, 884, 1181, 1190, 1425
HpyF3I CTNAG 8 cut(s) 34, 169, 351, 356, 477, 927, 1021, 1083
HpySE526I ACGT 4 cut(s) 142, 148, 154, 1440
Hsp92I GRCGYC 3 cut(s) 148, 1001, 1440
Hsp92II CATG 5 cut(s) 691, 886, 1039, 1127, 1280
HspAI GCGC 2 cut(s) 1113, 1463
Kpn2I TCCGGA 1 cut(s) 703
Kzo9I GATC 2 cut(s) 1066, 1370
LmnI GCTCC 4 cut(s) 178, 203, 421, 916
Lsp1109I GCAGC 3 cut(s) 1312, 1348, 1437
LweI GCATC 6 cut(s) 186, 298, 649, 729, 854, 1270
MaeI CTAG 1 cut(s) 1382
MaeII ACGT 4 cut(s) 142, 148, 154, 1440
MaeIII GTNAC 5 cut(s) 39, 280, 600, 690, 1436
MalI GATC 2 cut(s) 1068, 1372
MboI GATC 2 cut(s) 1066, 1370
MboII GAAGA 7 cut(s) 105, 229, 311, 453, 664, 690, 1457
MflI RGATCY 1 cut(s) 1370
MhlI GDGCHC 3 cut(s) 107, 1142, 1155
MluCI AATT 5 cut(s) 447, 643, 698, 918, 1074
MlyI GAGTC 3 cut(s) 193, 497, 608
MmeI TCCRAC 4 cut(s) 212, 316, 765, 1261
MroI TCCGGA 1 cut(s) 703
MseI TTAA 3 cut(s) 582, 642, 1062
MslI CAYNNNNRTG 4 cut(s) 287, 759, 809, 1128
MspA1I CMGCKG 2 cut(s) 355, 866
MspCI CTTAAG 1 cut(s) 581
MspI CCGG 4 cut(s) 176, 596, 635, 704
MspR9I CCNGG 5 cut(s) 201, 275, 596, 711, 1295
MvaI CCWGG 4 cut(s) 201, 275, 711, 1295
MwoI GCNNNNNNNGC 6 cut(s) 308, 842, 884, 1181, 1190, 1425
NciI CCSGG 1 cut(s) 596
NdeII GATC 2 cut(s) 1066, 1370
NlaIII CATG 5 cut(s) 691, 886, 1039, 1127, 1280
NlaIV GGNNCC 2 cut(s) 708, 912
NmuCI GTSAC 4 cut(s) 280, 600, 690, 1436
NsbI TGCGCA 1 cut(s) 1464
NspV TTCGAA 1 cut(s) 1302
OliI CACNNNNGTG 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 1148
PagI TCATGA 2 cut(s) 1123, 1276
PaqCI CACCTGC 1 cut(s) 1468
PfeI GAWTC 6 cut(s) 87, 591, 628, 797, 1094, 1226
PfoI TCCNGGA 1 cut(s) 594
PkrI GCNGC 3 cut(s) 1327, 1363, 1427
PleI GAGTC 3 cut(s) 192, 496, 607
PpsI GAGTC 3 cut(s) 192, 496, 607
Ppu21I YACGTR 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 1155
Psp6I CCWGG 4 cut(s) 199, 273, 709, 1293
PspEI GGTNACC 1 cut(s) 39
PspGI CCWGG 4 cut(s) 199, 273, 709, 1293
PspN4I GGNNCC 2 cut(s) 708, 912
PspPI GGNCC 3 cut(s) 664, 818, 1253
PstI CTGCAG 1 cut(s) 1366
PstNI CAGNNNCTG 2 cut(s) 206, 878
PsuI RGATCY 1 cut(s) 1370
PvuII CAGCTG 2 cut(s) 355, 866
RsaI GTAC 2 cut(s) 241, 952
RsaNI GTAC 2 cut(s) 240, 951
RseI CAYNNNNRTG 4 cut(s) 287, 759, 809, 1128
SacI GAGCTC 1 cut(s) 1155
SaqAI TTAA 3 cut(s) 582, 642, 1062
SatI GCNGC 3 cut(s) 1326, 1362, 1426
Sau3AI GATC 2 cut(s) 1066, 1370
Sau96I GGNCC 3 cut(s) 664, 818, 1253
SchI GAGTC 3 cut(s) 193, 497, 608
ScrFI CCNGG 5 cut(s) 201, 275, 596, 711, 1295
SduI GDGCHC 3 cut(s) 107, 1142, 1155
SfaNI GCATC 6 cut(s) 186, 298, 649, 729, 854, 1270
SfcI CTRYAG 3 cut(s) 374, 549, 1362
Sfr274I CTCGAG 1 cut(s) 1148
SfuI TTCGAA 1 cut(s) 1302
SinI GGWCC 1 cut(s) 1253
SlaI CTCGAG 1 cut(s) 1148
SmiMI CAYNNNNRTG 4 cut(s) 287, 759, 809, 1128
SmlI CTYRAG 3 cut(s) 581, 673, 1148
SmoI CTYRAG 3 cut(s) 581, 673, 1148
Sse9I AATT 5 cut(s) 447, 643, 698, 918, 1074
SsiI CCGC 5 cut(s) 419, 458, 496, 662, 1283
SspMI CTAG 1 cut(s) 1382
SstI GAGCTC 1 cut(s) 1155
StyD4I CCNGG 5 cut(s) 199, 273, 594, 709, 1293
TaaI ACNGT 5 cut(s) 193, 212, 553, 808, 1033
TaiI ACGT 4 cut(s) 145, 151, 157, 1443
TaqI TCGA 4 cut(s) 85, 1149, 1302, 1443
TaqII GACCGA 1 cut(s) 1241
TasI AATT 5 cut(s) 447, 643, 698, 918, 1074
TatI WGTACW 2 cut(s) 239, 950
TfiI GAWTC 6 cut(s) 87, 591, 628, 797, 1094, 1226
Tru1I TTAA 3 cut(s) 582, 642, 1062
Tru9I TTAA 3 cut(s) 582, 642, 1062
TscAI CASTG 2 cut(s) 289, 349
TseFI GTSAC 4 cut(s) 280, 600, 690, 1436
TseI GCWGC 3 cut(s) 1325, 1361, 1425
Tsp45I GTSAC 4 cut(s) 280, 600, 690, 1436
TspDTI ATGAA 7 cut(s) 43, 146, 276, 981, 1119, 1257, 1265
TspGWI ACGGA 1 cut(s) 1336
TspRI CASTG 2 cut(s) 289, 349
Vha464I CTTAAG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 1253
XagI CCTNNNNNAGG 1 cut(s) 1220
XapI RAATTY 3 cut(s) 447, 918, 1074
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 1148
XmiI GTMKAC 1 cut(s) 491
XspI CTAG 1 cut(s) 1382
ZraI GACGTC 2 cut(s) 149, 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.