Rh5AG030400

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001372
Physical Location & Seq
Reverse (-)
2111170 .. 2111715
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG030400.1

Sequence Viewer

Length: 546 bp
ATGAGCATGCCTGCAATACCAGGACCTGACAGTTTGAAGAAAAACAAGCAGAGTACGGGTACTAATGATGATGTCGAAGATGAAATGGTGGGTCGGGTGGTTTCTGGTGTTATTGAAGGTTCATTTGTTGCCGGCTATCTTCTGAATGTTGAGGTAGAAGTCACAGACACTCAGCTGAGGGCTGTTGTATTTCTGCCAGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCCATCTCATCTTTATGGTTCTGCCCGCCCATCAGAGCAAAGTAACAGGCAACCTTCTGACATGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCGTATTCCTCAGTGTGGCATCCCGGAGTCTCTTAGAAGTCAATCTTTCTCTGGCATCCTGGAGTCTCTTAGCCAACGCATGTCAGAGTCTCTTAGAAGTCGATCTTTCTCTGGCATTCCGGAGTCTCTTAGAAGCCAACCAACCTCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.48

Weight (kDa)

5.19

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 297, 376
AccIII TCCGGA 1 cut(s) 510
AciI CCGC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 2 cut(s) 55, 61
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 2 cut(s) 37, 116
AjnI CCWGG 3 cut(s) 19, 196, 449
AluBI AGCT 2 cut(s) 175, 281
AluI AGCT 2 cut(s) 175, 281
Alw26I GTCTC 4 cut(s) 425, 461, 485, 521
AlwNI CAGNNNCTG 2 cut(s) 26, 218
Aor13HI TCCGGA 1 cut(s) 510
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 415
AvaII GGWCC 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbvCI CCTCAGC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 233
BccI CCATC 2 cut(s) 301, 328
BciT130I CCWGG 3 cut(s) 21, 198, 451
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 4 cut(s) 425, 461, 485, 521
BfmI CTRYAG 2 cut(s) 219, 369
BfuAI ACCTGC 2 cut(s) 297, 376
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 4 cut(s) 21, 198, 415, 451
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 4 cut(s) 21, 198, 415, 451
BmsI GCATC 2 cut(s) 419, 455
BpmI CTGGAG 2 cut(s) 195, 472
Bpu10I CCTNAGC 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 415
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 510
BseBI CCWGG 3 cut(s) 21, 198, 451
BseGI GGATG 2 cut(s) 410, 446
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 3 cut(s) 167, 185, 415
BseNI ACTGG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 233
BsiSI CCGG 3 cut(s) 132, 415, 511
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 4 cut(s) 425, 461, 485, 521
BsmI GAATGC 1 cut(s) 506
Bsp13I TCCGGA 1 cut(s) 510
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 1 cut(s) 316
BspCNI CTCAG 3 cut(s) 168, 184, 414
BspEI TCCGGA 1 cut(s) 510
BspMAI CTGCAG 2 cut(s) 223, 373
BspMI ACCTGC 2 cut(s) 297, 376
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 131
BssMI GATC 1 cut(s) 493
Bst2UI CCWGG 3 cut(s) 21, 198, 451
Bst4CI ACNGT 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 8, 12, 133, 316
BstDEI CTNAG 7 cut(s) 171, 176, 401, 424, 460, 484, 520
BstF5I GGATG 2 cut(s) 410, 446
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 4 cut(s) 425, 461, 485, 521
BstMBI GATC 1 cut(s) 493
BstNI CCWGG 3 cut(s) 21, 198, 451
BstNSI RCATGY 2 cut(s) 10, 474
BstSCI CCNGG 4 cut(s) 19, 196, 413, 449
BstSFI CTRYAG 2 cut(s) 219, 369
BstV1I GCAGC 1 cut(s) 233
BtsCI GGATG 2 cut(s) 410, 446
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 216, 409
BveI ACCTGC 2 cut(s) 297, 376
Cac8I GCNNGC 4 cut(s) 8, 12, 133, 316
CaiI CAGNNNCTG 2 cut(s) 26, 218
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 54, 60
CviAII CATG 3 cut(s) 7, 352, 471
CviJI RGCY 6 cut(s) 135, 175, 182, 281, 464, 527
CviKI_1 RGCY 6 cut(s) 135, 175, 182, 281, 464, 527
CviQI GTAC 2 cut(s) 54, 60
DdeI CTNAG 7 cut(s) 171, 176, 401, 424, 460, 484, 520
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 23
EcoRII CCWGG 3 cut(s) 19, 196, 449
FaeI CATG 3 cut(s) 10, 355, 474
FaiI YATR 4 cut(s) 8, 306, 353, 472
FalI AAGNNNNNCTT 4 cut(s) 420, 452, 480, 512
FatI CATG 3 cut(s) 6, 351, 470
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 397, 433
Fsp4HI GCNGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 222
GsuI CTGGAG 2 cut(s) 195, 472
HapII CCGG 3 cut(s) 132, 415, 511
Hin1II CATG 3 cut(s) 10, 355, 474
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 4 cut(s) 418, 454, 478, 514
HpaII CCGG 3 cut(s) 132, 415, 511
Hpy166II GTNNAC 2 cut(s) 207, 286
Hpy188I TCNGA 4 cut(s) 144, 325, 349, 477
Hpy188III TCNNGA 2 cut(s) 377, 511
Hpy8I GTNNAC 2 cut(s) 207, 286
HpyAV CCTTC 2 cut(s) 110, 354
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4V TGCA 4 cut(s) 14, 221, 255, 371
HpyF3I CTNAG 7 cut(s) 171, 176, 401, 424, 460, 484, 520
Hsp92II CATG 3 cut(s) 10, 355, 474
Kpn2I TCCGGA 1 cut(s) 510
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 419, 455
MaeIII GTNAC 3 cut(s) 160, 214, 332
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 3 cut(s) 49, 89, 131
MluCI AATT 1 cut(s) 384
MlyI GAGTC 4 cut(s) 427, 463, 487, 523
MnlI CCTC 4 cut(s) 145, 171, 410, 546
MroI TCCGGA 1 cut(s) 510
MroNI GCCGGC 1 cut(s) 131
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 2 cut(s) 175, 281
MspI CCGG 3 cut(s) 132, 415, 511
MspR9I CCNGG 4 cut(s) 21, 198, 415, 451
Mva1269I GAATGC 1 cut(s) 506
MvaI CCWGG 3 cut(s) 21, 198, 451
NaeI GCCGGC 1 cut(s) 133
NciI CCSGG 1 cut(s) 415
NdeII GATC 1 cut(s) 493
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 3 cut(s) 10, 355, 474
NmuCI GTSAC 2 cut(s) 160, 214
NspI RCATGY 2 cut(s) 10, 474
PaeI GCATGC 1 cut(s) 10
PctI GAATGC 1 cut(s) 506
PdiI GCCGGC 1 cut(s) 133
PfoI TCCNGGA 2 cut(s) 413, 449
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 4 cut(s) 426, 462, 486, 522
PpsI GAGTC 4 cut(s) 426, 462, 486, 522
PpuMI RGGWCCY 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 23
Psp6I CCWGG 3 cut(s) 19, 196, 449
PspGI CCWGG 3 cut(s) 19, 196, 449
PspPI GGNCC 1 cut(s) 23
PspPPI RGGWCCY 1 cut(s) 23
PstI CTGCAG 2 cut(s) 223, 373
PstNI CAGNNNCTG 2 cut(s) 26, 218
PvuII CAGCTG 2 cut(s) 175, 281
RsaI GTAC 2 cut(s) 55, 61
RsaNI GTAC 2 cut(s) 54, 60
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 4 cut(s) 427, 463, 487, 523
ScrFI CCNGG 4 cut(s) 21, 198, 415, 451
SetI ASST 9 cut(s) 28, 121, 156, 177, 283, 291, 346, 370, 538
SfaNI GCATC 2 cut(s) 419, 455
SfcI CTRYAG 2 cut(s) 219, 369
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 303
SphI GCATGC 1 cut(s) 10
Sse9I AATT 1 cut(s) 384
SsiI CCGC 1 cut(s) 316
StyD4I CCNGG 4 cut(s) 19, 196, 413, 449
TaaI ACNGT 1 cut(s) 32
TaqI TCGA 2 cut(s) 75, 492
TasI AATT 1 cut(s) 384
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 2 cut(s) 223, 409
TseFI GTSAC 2 cut(s) 160, 214
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 2 cut(s) 160, 214
TspDTI ATGAA 3 cut(s) 96, 111, 368
TspRI CASTG 2 cut(s) 223, 409
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 1 cut(s) 384
XceI RCATGY 2 cut(s) 10, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.