Prupe.4G022600_v2.0.a1

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001372
Physical Location & Seq
Forward (+)
1067557 .. 1072445
4889 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G022600.3

Sequence Viewer

Length: 1488 bp
ATGTGCAGCATGAATCAACAGAGTCAAGGAACGAGCTCTTTATCTTCGCCGGACCCCCCTATGAAGCGAAAACGTGGACGCCCACGTAAGGATGAGAATCTTGTTCATGCAGAGAGCGCGACTGCAGGGCCTGGAGCTGAAAGTTTGAAGAAAAACAAACAGAGTACAAGTGATGATGTTGAGAGTGAAATGGTAGGTCAGGTAGTCACTGGAGTGATTGAAGGTTCATTTGATGCTGGCTATCTTCTCAATGTCAGGGTAGGAGACACCGACACTCAGCTGCGGGGTGTTGTATTTCTACAAGAGAAAATCGCTCCAATTACTCCAGCAAATGACATTGCTCCTCATATTAAGATGTGCAAAAGAAAAGATATTCCCATACCGCCTCTCAACCTGCAATCTCAGCTTTATGGTTCTGCCCGCCCATCAGAGCAAAGTAATAGGCAACCTGCTGAGGTGAAAAACCAAACACCTACAGTTCCAGACCAAACTCAATCCTCTATCCCTGGCATACCGGAGTCACTTGAAAGCAAATCTTTCTCCGTTATGATTCCTCCTGTTGATAATTTGCCGAAGAATGATGTAGGCCCTTCATTGGAAGGAAAAGTCATGCCACAACAAATTTTGGAGCCTGTAACTCATAGCCAATCTGCCCCTGCGATGGGACAGTCAGAGCTTAATAACATTGTTGAACAGAATAAAGTGTTGGAGGAAGTTGAAGCAGCCGAAATAATGGAAGGACCAAATAGCACAGAGGCCAACAAAGCATCAAAAACCGAATCAGCATCTGAACCAGTTGCTGACATACCAGGCATTGACGCGGTATGTAAGGATCCTGAAATTCAAACTCAGGCTGTGGATTCTGAAAAGAGTGCATTGGTTCATGATGAAGTAAGGAGCCTTGATCTTGAACTCAATCAAACTCCTGTAGTTGCTGAACCTGCAGCAACTGCTGTCATTCCAGCTGATGAAACCGTAGGTAAGGATCCTCAAATTCAACCTCAGGGTGTGGATTCTGAAAAGAGCGCATTGGTTCGTGATGATGTAAAGAGCCTGAATCTTGAACTCAACCAAATTCCTGTATTTTCTGAATCTGCACCAGTTTCTGTGATACCAGGCATTGAAACCATATGTAAGGATTCTCAAATTCAAGCTGTGGATGTGGGTCCTGAAAAGAATGCATTGGTTCATGAAGTGGCAAAGAGCCTGAATATTGAACTCAACCAAACTCCTGTATTTGCTGAACCTGCATGCGTTTTTTCTGATCTAGCCAGTAAACCAGTTGACATTGTGATGGAAGAACAGGCTTACCCTGCGCCAAAGCCCAGCCAGATGTTTGGGGGAGAGACTGTTCCATCTGAATTGAAGCTTGCATCTGAAGGATCTATTCCTCCTGGAATGGTTGAACCCCATATCTCTAGTTCTTCTGGTGCCATTAGCAATGTGGATTGTGATGTCGAAGATGTCATTCCACCTACACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

496

Amino Acids

52.72

Weight (kDa)

4.49

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 402, 457, 949, 1255
AccB1I GGYRCC 1 cut(s) 1430
AccII CGCG 2 cut(s) 119, 821
AciI CCGC 4 cut(s) 283, 383, 421, 821
AclWI GGATC 5 cut(s) 827, 840, 980, 993, 1390
AcsI RAATTY 5 cut(s) 621, 840, 993, 1074, 1146
AcuI CTGAAG 1 cut(s) 1398
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 4 cut(s) 88, 595, 661, 662
AjnI CCWGG 5 cut(s) 130, 505, 808, 1114, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AleI CACNNNNGTG 1 cut(s) 212
AloI GAACNNNNNNTCC 2 cut(s) 1335, 1367
AluBI AGCT 8 cut(s) 36, 137, 280, 406, 676, 965, 1154, 1369
AluI AGCT 8 cut(s) 36, 137, 280, 406, 676, 965, 1154, 1369
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 2 cut(s) 258, 1340
AlwI GGATC 5 cut(s) 827, 840, 980, 993, 1390
AlwNI CAGNNNCTG 5 cut(s) 131, 788, 800, 950, 1106
AoxI GGCC 3 cut(s) 128, 586, 756
ApeKI GCWGC 4 cut(s) 6, 280, 722, 944
ApoI RAATTY 5 cut(s) 621, 840, 993, 1074, 1146
AspLEI GCGC 3 cut(s) 119, 1028, 1318
AspS9I GGNCC 5 cut(s) 52, 128, 587, 740, 1166
AsuHPI GGTGA 1 cut(s) 469
AvaII GGWCC 3 cut(s) 52, 740, 1166
AxyI CCTNAGG 1 cut(s) 1002
BamHI GGATCC 2 cut(s) 832, 985
BanI GGYRCC 1 cut(s) 1430
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 4 cut(s) 18, 267, 734, 956
BccI CCATC 4 cut(s) 433, 655, 1288, 1363
BciT130I CCWGG 5 cut(s) 132, 507, 810, 1116, 1395
BcoDI GTCTC 2 cut(s) 258, 1340
BfaI CTAG 2 cut(s) 1268, 1419
BfmI CTRYAG 4 cut(s) 123, 474, 927, 942
BfuAI ACCTGC 4 cut(s) 402, 457, 949, 1255
BisI GCNGC 4 cut(s) 7, 281, 723, 945
BlsI GCNGC 4 cut(s) 8, 282, 724, 946
Bme1390I CCNGG 5 cut(s) 132, 507, 810, 1116, 1395
Bme18I GGWCC 3 cut(s) 52, 740, 1166
BmgT120I GGNCC 5 cut(s) 52, 128, 587, 740, 1166
BmiI GGNNCC 7 cut(s) 54, 630, 834, 899, 987, 1167, 1432
BmrFI CCNGG 5 cut(s) 132, 507, 810, 1116, 1395
BmsI GCATC 4 cut(s) 223, 776, 794, 1382
BpmI CTGGAG 3 cut(s) 153, 231, 309
Bpu10I CCTNAGC 1 cut(s) 453
BsaAI YACGTR 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 505
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 1335, 1365
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 595, 661, 662
Bse1I ACTGG 5 cut(s) 214, 794, 1100, 1272, 1280
Bse21I CCTNAGG 1 cut(s) 1002
Bse3DI GCAATG 2 cut(s) 336, 1447
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 5 cut(s) 132, 507, 810, 1116, 1395
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 2 cut(s) 97, 1165
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 4 cut(s) 88, 595, 661, 662
BseMI GCAATG 2 cut(s) 336, 1447
BseMII CTCAG 5 cut(s) 290, 416, 444, 863, 1016
BseNI ACTGG 5 cut(s) 214, 794, 1100, 1272, 1280
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 4 cut(s) 18, 267, 734, 956
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 2 cut(s) 25, 1080
Bsh1236I CGCG 2 cut(s) 119, 821
BshFI GGCC 3 cut(s) 130, 588, 758
BshNI GGYRCC 1 cut(s) 1430
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 2 cut(s) 50, 515
BslFI GGGAC 1 cut(s) 678
BslI CCNNNNNNNGG 4 cut(s) 88, 595, 661, 662
BsmAI GTCTC 2 cut(s) 258, 1340
BsmFI GGGAC 1 cut(s) 678
BsmI GAATGC 1 cut(s) 1183
BsnI GGCC 3 cut(s) 130, 588, 758
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 5 cut(s) 832, 904, 985, 1264, 1382
BspACI CCGC 4 cut(s) 283, 383, 421, 821
BspANI GGCC 3 cut(s) 130, 588, 758
BspCNI CTCAG 5 cut(s) 289, 415, 445, 862, 1015
BspFNI CGCG 2 cut(s) 119, 821
BspHI TCATGA 2 cut(s) 883, 1189
BspLI GGNNCC 7 cut(s) 54, 630, 834, 899, 987, 1167, 1432
BspMAI CTGCAG 2 cut(s) 127, 946
BspMI ACCTGC 4 cut(s) 402, 457, 949, 1255
BspPI GGATC 5 cut(s) 827, 840, 980, 993, 1390
BspT107I GGYRCC 1 cut(s) 1430
BsrDI GCAATG 2 cut(s) 336, 1447
BsrI ACTGG 5 cut(s) 214, 794, 1100, 1272, 1280
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 5 cut(s) 832, 904, 985, 1264, 1382
BssNI GRCGYC 1 cut(s) 79
Bst2UI CCWGG 5 cut(s) 132, 507, 810, 1116, 1395
Bst4CI ACNGT 4 cut(s) 478, 669, 976, 1351
BstACI GRCGYC 1 cut(s) 79
BstAPI GCANNNNNTGC 1 cut(s) 950
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 4 cut(s) 238, 421, 1252, 1371
BstDEI CTNAG 6 cut(s) 276, 402, 453, 849, 1002, 1485
BstF5I GGATG 2 cut(s) 97, 1165
BstFNI CGCG 2 cut(s) 119, 821
BstHHI GCGC 3 cut(s) 119, 1028, 1318
BstKTI GATC 5 cut(s) 835, 907, 988, 1267, 1385
BstMAI GTCTC 2 cut(s) 258, 1340
BstMBI GATC 5 cut(s) 832, 904, 985, 1264, 1382
BstMWI GCNNNNNNNGC 7 cut(s) 116, 403, 764, 941, 950, 1247, 1313
BstNI CCWGG 5 cut(s) 132, 507, 810, 1116, 1395
BstNSI RCATGY 1 cut(s) 1254
BstSCI CCNGG 5 cut(s) 130, 505, 808, 1114, 1393
BstSFI CTRYAG 4 cut(s) 123, 474, 927, 942
BstUI CGCG 2 cut(s) 119, 821
BstV1I GCAGC 4 cut(s) 18, 267, 734, 956
BstX2I RGATCY 3 cut(s) 832, 985, 1382
BstXI CCANNNNNNTGG 1 cut(s) 1337
BstYI RGATCY 3 cut(s) 832, 985, 1382
Bsu36I CCTNAGG 1 cut(s) 1002
BsuRI GGCC 3 cut(s) 130, 588, 758
BtgZI GCGATG 1 cut(s) 674
BtsCI GGATG 2 cut(s) 97, 1165
BtsIMutI CAGTG 1 cut(s) 207
BveI ACCTGC 4 cut(s) 402, 457, 949, 1255
Cac8I GCNNGC 4 cut(s) 238, 421, 1252, 1371
CaiI CAGNNNCTG 5 cut(s) 131, 788, 800, 950, 1106
CciI TCATGA 2 cut(s) 883, 1189
CfoI GCGC 3 cut(s) 119, 1028, 1318
Cfr13I GGNCC 5 cut(s) 52, 128, 587, 740, 1166
CseI GACGC 2 cut(s) 87, 827
Csp6I GTAC 1 cut(s) 165
CviAII CATG 6 cut(s) 10, 107, 610, 884, 1190, 1251
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 6 cut(s) 276, 402, 453, 849, 1002, 1485
DpnI GATC 5 cut(s) 834, 906, 987, 1266, 1384
DpnII GATC 5 cut(s) 832, 904, 985, 1264, 1382
Ecl136II GAGCTC 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 3 cut(s) 52, 740, 1166
Eco53kI GAGCTC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 1398
Eco81I CCTNAGG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 36
EcoO109I RGGNCCY 3 cut(s) 128, 587, 1166
EcoRII CCWGG 5 cut(s) 130, 505, 808, 1114, 1393
EcoT22I ATGCAT 1 cut(s) 1183
EcoT38I GRGCYC 1 cut(s) 38
FaeI CATG 6 cut(s) 13, 110, 613, 887, 1193, 1254
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FaqI GGGAC 1 cut(s) 678
FatI CATG 6 cut(s) 9, 106, 609, 883, 1189, 1250
FauI CCCGC 2 cut(s) 276, 428
FauNDI CATATG 1 cut(s) 1130
Fnu4HI GCNGC 4 cut(s) 7, 281, 723, 945
FokI GGATG 2 cut(s) 104, 1172
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 4 cut(s) 7, 281, 723, 945
FspBI CTAG 2 cut(s) 1268, 1419
GlaI GCGC 3 cut(s) 118, 1027, 1317
GluI GCNGC 4 cut(s) 7, 281, 723, 945
GsaI CCCAGC 1 cut(s) 1329
GsuI CTGGAG 3 cut(s) 153, 231, 309
HaeIII GGCC 3 cut(s) 130, 588, 758
HapII CCGG 2 cut(s) 50, 515
HgaI GACGC 2 cut(s) 87, 827
HhaI GCGC 3 cut(s) 119, 1028, 1318
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 6 cut(s) 13, 110, 613, 887, 1193, 1254
Hin6I GCGC 3 cut(s) 117, 1026, 1316
HinP1I GCGC 3 cut(s) 117, 1026, 1316
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HindIII AAGCTT 1 cut(s) 1367
HpaII CCGG 2 cut(s) 50, 515
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 3 cut(s) 77, 1277, 1285
Hpy188I TCNGA 9 cut(s) 430, 673, 790, 865, 1018, 1090, 1264, 1360, 1378
Hpy188III TCNNGA 8 cut(s) 482, 836, 884, 908, 1037, 1061, 1169, 1190
Hpy8I GTNNAC 3 cut(s) 77, 1277, 1285
HpyAV CCTTC 5 cut(s) 215, 593, 600, 731, 1373
HpyCH4III ACNGT 4 cut(s) 478, 669, 976, 1351
HpyCH4IV ACGT 2 cut(s) 73, 85
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 403, 764, 941, 950, 1247, 1313
HpyF3I CTNAG 6 cut(s) 276, 402, 453, 849, 1002, 1485
HpySE526I ACGT 2 cut(s) 73, 85
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 6 cut(s) 13, 110, 613, 887, 1193, 1254
HspAI GCGC 3 cut(s) 117, 1026, 1316
Kzo9I GATC 5 cut(s) 832, 904, 985, 1264, 1382
LmnI GCTCC 5 cut(s) 134, 319, 346, 628, 897
Lsp1109I GCAGC 4 cut(s) 18, 267, 734, 956
LweI GCATC 4 cut(s) 223, 776, 794, 1382
MaeI CTAG 2 cut(s) 1268, 1419
MaeII ACGT 2 cut(s) 73, 85
MaeIII GTNAC 3 cut(s) 205, 519, 634
MalI GATC 5 cut(s) 834, 906, 987, 1266, 1384
MboI GATC 5 cut(s) 832, 904, 985, 1264, 1382
MboII GAAGA 7 cut(s) 36, 160, 236, 586, 1310, 1416, 1472
MflI RGATCY 3 cut(s) 832, 985, 1382
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 8 cut(s) 318, 565, 621, 840, 993, 1074, 1146, 1361
MlyI GAGTC 2 cut(s) 31, 527
MmeI TCCRAC 1 cut(s) 687
Mph1103I ATGCAT 1 cut(s) 1183
MseI TTAA 2 cut(s) 351, 678
MslI CAYNNNNRTG 2 cut(s) 212, 1292
MspA1I CMGCKG 2 cut(s) 280, 965
MspI CCGG 2 cut(s) 50, 515
MspR9I CCNGG 5 cut(s) 132, 507, 810, 1116, 1395
Mva1269I GAATGC 1 cut(s) 1183
MvaI CCWGG 5 cut(s) 132, 507, 810, 1116, 1395
MvnI CGCG 2 cut(s) 119, 821
MwoI GCNNNNNNNGC 7 cut(s) 116, 403, 764, 941, 950, 1247, 1313
NdeI CATATG 1 cut(s) 1130
NdeII GATC 5 cut(s) 832, 904, 985, 1264, 1382
NlaIII CATG 6 cut(s) 13, 110, 613, 887, 1193, 1254
NlaIV GGNNCC 7 cut(s) 54, 630, 834, 899, 987, 1167, 1432
NmuCI GTSAC 2 cut(s) 205, 519
NsiI ATGCAT 1 cut(s) 1183
NspI RCATGY 1 cut(s) 1254
OliI CACNNNNGTG 1 cut(s) 212
PaeI GCATGC 1 cut(s) 1254
PagI TCATGA 2 cut(s) 883, 1189
PctI GAATGC 1 cut(s) 1183
PfeI GAWTC 9 cut(s) 13, 97, 550, 779, 860, 1013, 1057, 1091, 1139
PfoI TCCNGGA 1 cut(s) 1393
PkrI GCNGC 4 cut(s) 8, 282, 724, 946
PleI GAGTC 2 cut(s) 30, 526
PpsI GAGTC 2 cut(s) 30, 526
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 1166
Psp124BI GAGCTC 1 cut(s) 38
Psp5II RGGWCCY 1 cut(s) 1166
Psp6I CCWGG 5 cut(s) 130, 505, 808, 1114, 1393
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 5 cut(s) 130, 505, 808, 1114, 1393
PspN4I GGNNCC 7 cut(s) 54, 630, 834, 899, 987, 1167, 1432
PspPI GGNCC 5 cut(s) 52, 128, 587, 740, 1166
PspPPI RGGWCCY 1 cut(s) 1166
PstI CTGCAG 2 cut(s) 127, 946
PstNI CAGNNNCTG 5 cut(s) 131, 788, 800, 950, 1106
PsuI RGATCY 3 cut(s) 832, 985, 1382
PvuII CAGCTG 2 cut(s) 280, 965
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 212, 1292
SacI GAGCTC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 351, 678
SatI GCNGC 4 cut(s) 7, 281, 723, 945
Sau3AI GATC 5 cut(s) 832, 904, 985, 1264, 1382
Sau96I GGNCC 5 cut(s) 52, 128, 587, 740, 1166
SchI GAGTC 2 cut(s) 31, 527
ScrFI CCNGG 5 cut(s) 132, 507, 810, 1116, 1395
SduI GDGCHC 1 cut(s) 38
SfaNI GCATC 4 cut(s) 223, 776, 794, 1382
SfcI CTRYAG 4 cut(s) 123, 474, 927, 942
SinI GGWCC 3 cut(s) 52, 740, 1166
SmiMI CAYNNNNRTG 2 cut(s) 212, 1292
SphI GCATGC 1 cut(s) 1254
Sse9I AATT 8 cut(s) 318, 565, 621, 840, 993, 1074, 1146, 1361
SsiI CCGC 4 cut(s) 283, 383, 421, 821
SspI AATATT 1 cut(s) 1213
SspMI CTAG 2 cut(s) 1268, 1419
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 5 cut(s) 130, 505, 808, 1114, 1393
TaaI ACNGT 4 cut(s) 478, 669, 976, 1351
TaiI ACGT 2 cut(s) 76, 88
TaqI TCGA 1 cut(s) 1458
TasI AATT 8 cut(s) 318, 565, 621, 840, 993, 1074, 1146, 1361
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 9 cut(s) 13, 97, 550, 779, 860, 1013, 1057, 1091, 1139
Tru1I TTAA 2 cut(s) 351, 678
Tru9I TTAA 2 cut(s) 351, 678
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 2 cut(s) 205, 519
TseI GCWGC 4 cut(s) 6, 280, 722, 944
Tsp45I GTSAC 2 cut(s) 205, 519
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 3 cut(s) 52, 740, 1166
XapI RAATTY 5 cut(s) 621, 840, 993, 1074, 1146
XceI RCATGY 1 cut(s) 1254
XcmI CCANNNNNNNNNTGG 1 cut(s) 1441
XspI CTAG 2 cut(s) 1268, 1419
Zsp2I ATGCAT 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.