DNA binding domain with preference for A/T rich regions
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1426 |
| Acc36I | ACCTGC | 4 cut(s) | 291, 370, 1246, 1426 |
| AccB1I | GGYRCC | 1 cut(s) | 155 |
| AciI | CCGC | 2 cut(s) | 50, 1086 |
| AclI | AACGTT | 1 cut(s) | 1050 |
| AclWI | GGATC | 8 cut(s) | 611, 624, 815, 828, 1124, 1137, 1332, 1345 |
| AcoI | YGGCCR | 2 cut(s) | 311, 711 |
| AcsI | RAATTY | 4 cut(s) | 378, 892, 984, 1137 |
| AcuI | CTGAAG | 2 cut(s) | 945, 1332 |
| AfaI | GTAC | 2 cut(s) | 55, 795 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 401, 583, 584, 1370 |
| AgsI | TTSAA | 9 cut(s) | 110, 458, 680, 707, 833, 899, 962, 989, 1055 |
| AjnI | CCWGG | 4 cut(s) | 13, 471, 499, 619 |
| AluBI | AGCT | 4 cut(s) | 169, 275, 929, 1210 |
| AluI | AGCT | 4 cut(s) | 169, 275, 929, 1210 |
| Alw21I | GWGCWC | 1 cut(s) | 1212 |
| Alw26I | GTCTC | 5 cut(s) | 407, 456, 483, 519, 1109 |
| AlwI | GGATC | 8 cut(s) | 611, 624, 815, 828, 1124, 1137, 1332, 1345 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 20, 212, 1238 |
| AoxI | GGCC | 3 cut(s) | 311, 332, 711 |
| ApeKI | GCWGC | 4 cut(s) | 215, 929, 1247, 1286 |
| ApoI | RAATTY | 4 cut(s) | 378, 892, 984, 1137 |
| AspLEI | GCGC | 1 cut(s) | 1291 |
| AspS9I | GGNCC | 1 cut(s) | 17 |
| AsuC2I | CCSGG | 4 cut(s) | 192, 310, 410, 509 |
| AsuHPI | GGTGA | 5 cut(s) | 80, 358, 800, 1370, 1407 |
| AsuII | TTCGAA | 1 cut(s) | 29 |
| AvaII | GGWCC | 1 cut(s) | 17 |
| AxyI | CCTNAGG | 2 cut(s) | 837, 1146 |
| BamHI | GGATCC | 4 cut(s) | 616, 820, 1129, 1337 |
| BanI | GGYRCC | 1 cut(s) | 155 |
| BanII | GRGCYC | 1 cut(s) | 1212 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 252, 284 |
| Bbv12I | GWGCWC | 1 cut(s) | 1212 |
| BbvCI | CCTCAGC | 1 cut(s) | 170 |
| BbvI | GCAGC | 4 cut(s) | 227, 916, 1259, 1273 |
| BccI | CCATC | 6 cut(s) | 59, 295, 322, 535, 647, 1267 |
| BciT130I | CCWGG | 4 cut(s) | 15, 473, 501, 621 |
| BciVI | GTATCC | 1 cut(s) | 997 |
| BcnI | CCSGG | 4 cut(s) | 192, 310, 410, 509 |
| BcoDI | GTCTC | 5 cut(s) | 407, 456, 483, 519, 1109 |
| BfmI | CTRYAG | 2 cut(s) | 213, 1332 |
| BfuAI | ACCTGC | 4 cut(s) | 291, 370, 1246, 1426 |
| BfuI | GTATCC | 1 cut(s) | 997 |
| BisI | GCNGC | 4 cut(s) | 216, 930, 1248, 1287 |
| BlsI | GCNGC | 4 cut(s) | 217, 931, 1249, 1288 |
| Bme1390I | CCNGG | 8 cut(s) | 15, 192, 310, 410, 473, 501, 509, 621 |
| Bme18I | GGWCC | 1 cut(s) | 17 |
| BmgT120I | GGNCC | 1 cut(s) | 17 |
| BmiI | GGNNCC | 6 cut(s) | 157, 618, 822, 978, 1131, 1339 |
| BmrFI | CCNGG | 8 cut(s) | 15, 192, 310, 410, 473, 501, 509, 621 |
| BmrI | ACTGGG | 1 cut(s) | 1115 |
| BmsI | GCATC | 8 cut(s) | 112, 414, 450, 477, 513, 562, 1337, 1396 |
| BmuI | ACTGGG | 1 cut(s) | 1115 |
| BpmI | CTGGAG | 1 cut(s) | 189 |
| Bpu10I | CCTNAGC | 1 cut(s) | 170 |
| Bpu14I | TTCGAA | 1 cut(s) | 29 |
| BpuEI | CTTGAG | 1 cut(s) | 1226 |
| BpuMI | CCSGG | 4 cut(s) | 192, 310, 410, 509 |
| BsaJI | CCNNGG | 2 cut(s) | 499, 1353 |
| BsaWI | WCCGGW | 1 cut(s) | 547 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 579, 609, 1308, 1338 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 401, 583, 584, 1370 |
| Bse118I | RCCGGY | 2 cut(s) | 125, 709 |
| Bse1I | ACTGG | 5 cut(s) | 206, 739, 1092, 1110, 1259 |
| Bse21I | CCTNAGG | 2 cut(s) | 837, 1146 |
| Bse3DI | GCAATG | 2 cut(s) | 15, 945 |
| BseBI | CCWGG | 4 cut(s) | 15, 473, 501, 621 |
| BseDI | CCNNGG | 2 cut(s) | 499, 1353 |
| BseGI | GGATG | 8 cut(s) | 405, 441, 468, 504, 527, 639, 1156, 1189 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 401, 583, 584, 1370 |
| BseMI | GCAATG | 2 cut(s) | 15, 945 |
| BseMII | CTCAG | 6 cut(s) | 161, 179, 410, 851, 1007, 1137 |
| BseNI | ACTGG | 5 cut(s) | 206, 739, 1092, 1110, 1259 |
| BseRI | GAGGAG | 2 cut(s) | 969, 1212 |
| BseXI | GCAGC | 4 cut(s) | 227, 916, 1259, 1273 |
| BsgI | GTGCAG | 1 cut(s) | 1404 |
| BshFI | GGCC | 3 cut(s) | 313, 334, 713 |
| BshNI | GGYRCC | 1 cut(s) | 155 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1212 |
| BsiSI | CCGG | 7 cut(s) | 126, 191, 310, 410, 509, 548, 710 |
| BslFI | GGGAC | 1 cut(s) | 1333 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 401, 583, 584, 1370 |
| BsmAI | GTCTC | 5 cut(s) | 407, 456, 483, 519, 1109 |
| BsmFI | GGGAC | 1 cut(s) | 1333 |
| BsnI | GGCC | 3 cut(s) | 313, 334, 713 |
| Bsp119I | TTCGAA | 1 cut(s) | 29 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1212 |
| Bsp143I | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| Bsp19I | CCATGG | 1 cut(s) | 1353 |
| BspACI | CCGC | 2 cut(s) | 50, 1086 |
| BspANI | GGCC | 3 cut(s) | 313, 334, 713 |
| BspCNI | CTCAG | 6 cut(s) | 162, 178, 409, 850, 1006, 1138 |
| BspLI | GGNNCC | 6 cut(s) | 157, 618, 822, 978, 1131, 1339 |
| BspMAI | CTGCAG | 1 cut(s) | 217 |
| BspMI | ACCTGC | 4 cut(s) | 291, 370, 1246, 1426 |
| BspPI | GGATC | 8 cut(s) | 611, 624, 815, 828, 1124, 1137, 1332, 1345 |
| BspT104I | TTCGAA | 1 cut(s) | 29 |
| BspT107I | GGYRCC | 1 cut(s) | 155 |
| BsrDI | GCAATG | 2 cut(s) | 15, 945 |
| BsrFI | RCCGGY | 2 cut(s) | 125, 709 |
| BsrI | ACTGG | 5 cut(s) | 206, 739, 1092, 1110, 1259 |
| BssAI | RCCGGY | 2 cut(s) | 125, 709 |
| BssECI | CCNNGG | 2 cut(s) | 499, 1353 |
| BssMI | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| BssT1I | CCWWGG | 1 cut(s) | 1353 |
| Bst2UI | CCWGG | 4 cut(s) | 15, 473, 501, 621 |
| Bst4CI | ACNGT | 6 cut(s) | 811, 967, 1000, 1120, 1324, 1385 |
| Bst6I | CTCTTC | 2 cut(s) | 459, 1275 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1238 |
| BstBI | TTCGAA | 1 cut(s) | 29 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 127 |
| BstDSI | CCRYGG | 1 cut(s) | 1353 |
| BstF5I | GGATG | 8 cut(s) | 405, 441, 468, 504, 527, 639, 1156, 1189 |
| BstHHI | GCGC | 1 cut(s) | 1291 |
| BstKTI | GATC | 4 cut(s) | 619, 823, 1132, 1340 |
| BstMAI | GTCTC | 5 cut(s) | 407, 456, 483, 519, 1109 |
| BstMBI | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 10, 319, 929, 935, 1085, 1238, 1247 |
| BstNI | CCWGG | 4 cut(s) | 15, 473, 501, 621 |
| BstSCI | CCNGG | 8 cut(s) | 13, 190, 308, 408, 471, 499, 507, 619 |
| BstSFI | CTRYAG | 2 cut(s) | 213, 1332 |
| BstV1I | GCAGC | 4 cut(s) | 227, 916, 1259, 1273 |
| BstX2I | RGATCY | 4 cut(s) | 616, 820, 1129, 1337 |
| BstYI | RGATCY | 4 cut(s) | 616, 820, 1129, 1337 |
| Bsu36I | CCTNAGG | 2 cut(s) | 837, 1146 |
| BsuI | GTATCC | 1 cut(s) | 997 |
| BsuRI | GGCC | 3 cut(s) | 313, 334, 713 |
| BtgI | CCRYGG | 1 cut(s) | 1353 |
| BtsCI | GGATG | 8 cut(s) | 405, 441, 468, 504, 527, 639, 1156, 1189 |
| BtsI | GCAGTG | 2 cut(s) | 210, 865 |
| BtsIMutI | CAGTG | 3 cut(s) | 210, 404, 865 |
| BveI | ACCTGC | 4 cut(s) | 291, 370, 1246, 1426 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 127 |
| CaiI | CAGNNNCTG | 3 cut(s) | 20, 212, 1238 |
| CfoI | GCGC | 1 cut(s) | 1291 |
| Cfr10I | RCCGGY | 2 cut(s) | 125, 709 |
| Cfr13I | GGNCC | 1 cut(s) | 17 |
| Csp6I | GTAC | 2 cut(s) | 54, 794 |
| CviAII | CATG | 4 cut(s) | 644, 889, 1070, 1354 |
| CviQI | GTAC | 2 cut(s) | 54, 794 |
| DpnI | GATC | 4 cut(s) | 618, 822, 1131, 1339 |
| DpnII | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| EaeI | YGGCCR | 2 cut(s) | 311, 711 |
| Eam1104I | CTCTTC | 2 cut(s) | 459, 1275 |
| EarI | CTCTTC | 2 cut(s) | 459, 1275 |
| Ecl136II | GAGCTC | 1 cut(s) | 1210 |
| Eco130I | CCWWGG | 1 cut(s) | 1353 |
| Eco24I | GRGCYC | 1 cut(s) | 1212 |
| Eco47I | GGWCC | 1 cut(s) | 17 |
| Eco53kI | GAGCTC | 1 cut(s) | 1210 |
| Eco57I | CTGAAG | 2 cut(s) | 945, 1332 |
| Eco81I | CCTNAGG | 2 cut(s) | 837, 1146 |
| EcoICRI | GAGCTC | 1 cut(s) | 1210 |
| EcoO109I | RGGNCCY | 1 cut(s) | 17 |
| EcoRII | CCWGG | 4 cut(s) | 13, 471, 499, 619 |
| EcoT14I | CCWWGG | 1 cut(s) | 1353 |
| EcoT38I | GRGCYC | 1 cut(s) | 1212 |
| ErhI | CCWWGG | 1 cut(s) | 1353 |
| FaeI | CATG | 4 cut(s) | 647, 892, 1073, 1357 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 415, 447, 478, 510 |
| FaqI | GGGAC | 1 cut(s) | 1333 |
| FatI | CATG | 4 cut(s) | 643, 888, 1069, 1353 |
| FauI | CCCGC | 2 cut(s) | 43, 1093 |
| Fnu4HI | GCNGC | 4 cut(s) | 216, 930, 1248, 1287 |
| FokI | GGATG | 8 cut(s) | 392, 428, 455, 491, 514, 626, 1163, 1196 |
| FriOI | GRGCYC | 1 cut(s) | 1212 |
| Fsp4HI | GCNGC | 4 cut(s) | 216, 930, 1248, 1287 |
| GlaI | GCGC | 1 cut(s) | 1290 |
| GluI | GCNGC | 4 cut(s) | 216, 930, 1248, 1287 |
| GsuI | CTGGAG | 1 cut(s) | 189 |
| HaeIII | GGCC | 3 cut(s) | 313, 334, 713 |
| HapII | CCGG | 7 cut(s) | 126, 191, 310, 410, 509, 548, 710 |
| HhaI | GCGC | 1 cut(s) | 1291 |
| Hin1II | CATG | 4 cut(s) | 647, 892, 1073, 1357 |
| Hin6I | GCGC | 1 cut(s) | 1289 |
| HinP1I | GCGC | 1 cut(s) | 1289 |
| HincII | GTYRAC | 3 cut(s) | 280, 1264, 1381 |
| HindII | GTYRAC | 3 cut(s) | 280, 1264, 1381 |
| HinfI | GANTC | 8 cut(s) | 415, 449, 476, 512, 755, 767, 1015, 1300 |
| HpaII | CCGG | 7 cut(s) | 126, 191, 310, 410, 509, 548, 710 |
| HphI | GGTGA | 5 cut(s) | 80, 358, 800, 1370, 1407 |
| Hpy166II | GTNNAC | 6 cut(s) | 201, 280, 1048, 1264, 1361, 1381 |
| Hpy188I | TCNGA | 8 cut(s) | 138, 319, 661, 778, 853, 996, 1162, 1272 |
| Hpy188III | TCNNGA | 4 cut(s) | 371, 872, 1181, 1205 |
| Hpy8I | GTNNAC | 6 cut(s) | 201, 280, 1048, 1264, 1361, 1381 |
| Hpy99I | CGWCG | 1 cut(s) | 881 |
| HpyAV | CCTTC | 4 cut(s) | 104, 146, 1356, 1377 |
| HpyCH4III | ACNGT | 6 cut(s) | 811, 967, 1000, 1120, 1324, 1385 |
| HpyCH4IV | ACGT | 2 cut(s) | 879, 1050 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 10, 319, 929, 935, 1085, 1238, 1247 |
| HpySE526I | ACGT | 2 cut(s) | 879, 1050 |
| Hsp92II | CATG | 4 cut(s) | 647, 892, 1073, 1357 |
| HspAI | GCGC | 1 cut(s) | 1289 |
| KroI | GCCGGC | 1 cut(s) | 125 |
| KroNI | GCCGGC | 1 cut(s) | 127 |
| Kzo9I | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| LmnI | GCTCC | 2 cut(s) | 937, 982 |
| Lsp1109I | GCAGC | 4 cut(s) | 227, 916, 1259, 1273 |
| LweI | GCATC | 8 cut(s) | 112, 414, 450, 477, 513, 562, 1337, 1396 |
| MaeII | ACGT | 2 cut(s) | 879, 1050 |
| MaeIII | GTNAC | 3 cut(s) | 208, 326, 595 |
| MalI | GATC | 4 cut(s) | 618, 822, 1131, 1339 |
| MboI | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| MboII | GAAGA | 8 cut(s) | 43, 83, 125, 179, 446, 536, 1289, 1292 |
| MfeI | CAATTG | 2 cut(s) | 783, 1019 |
| MflI | RGATCY | 4 cut(s) | 616, 820, 1129, 1337 |
| MhlI | GDGCHC | 1 cut(s) | 1212 |
| MluCI | AATT | 9 cut(s) | 366, 378, 556, 783, 828, 892, 984, 1019, 1137 |
| MlyI | GAGTC | 6 cut(s) | 409, 458, 485, 521, 1024, 1309 |
| MmeI | TCCRAC | 1 cut(s) | 675 |
| MroNI | GCCGGC | 1 cut(s) | 125 |
| MseI | TTAA | 4 cut(s) | 144, 240, 1059, 1254 |
| MslI | CAYNNNNRTG | 1 cut(s) | 297 |
| MspA1I | CMGCKG | 2 cut(s) | 169, 275 |
| MspI | CCGG | 7 cut(s) | 126, 191, 310, 410, 509, 548, 710 |
| MspR9I | CCNGG | 8 cut(s) | 15, 192, 310, 410, 473, 501, 509, 621 |
| MunI | CAATTG | 2 cut(s) | 783, 1019 |
| MvaI | CCWGG | 4 cut(s) | 15, 473, 501, 621 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 10, 319, 929, 935, 1085, 1238, 1247 |
| NaeI | GCCGGC | 1 cut(s) | 127 |
| NciI | CCSGG | 4 cut(s) | 192, 310, 410, 509 |
| NcoI | CCATGG | 1 cut(s) | 1353 |
| NdeII | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| NgoMIV | GCCGGC | 1 cut(s) | 125 |
| NlaIII | CATG | 4 cut(s) | 647, 892, 1073, 1357 |
| NlaIV | GGNNCC | 6 cut(s) | 157, 618, 822, 978, 1131, 1339 |
| NmeAIII | GCCGAG | 1 cut(s) | 1323 |
| NmuCI | GTSAC | 2 cut(s) | 208, 595 |
| NspV | TTCGAA | 1 cut(s) | 29 |
| PaqCI | CACCTGC | 1 cut(s) | 1426 |
| PdiI | GCCGGC | 1 cut(s) | 127 |
| PfeI | GAWTC | 2 cut(s) | 755, 767 |
| PflFI | GACNNNGTC | 1 cut(s) | 655 |
| PfoI | TCCNGGA | 5 cut(s) | 190, 408, 471, 507, 619 |
| PkrI | GCNGC | 4 cut(s) | 217, 931, 1249, 1288 |
| PleI | GAGTC | 6 cut(s) | 409, 457, 484, 520, 1023, 1308 |
| PpsI | GAGTC | 6 cut(s) | 409, 457, 484, 520, 1023, 1308 |
| PpuMI | RGGWCCY | 1 cut(s) | 17 |
| Psp124BI | GAGCTC | 1 cut(s) | 1212 |
| Psp1406I | AACGTT | 1 cut(s) | 1050 |
| Psp5II | RGGWCCY | 1 cut(s) | 17 |
| Psp6I | CCWGG | 4 cut(s) | 13, 471, 499, 619 |
| PspGI | CCWGG | 4 cut(s) | 13, 471, 499, 619 |
| PspN4I | GGNNCC | 6 cut(s) | 157, 618, 822, 978, 1131, 1339 |
| PspPI | GGNCC | 1 cut(s) | 17 |
| PspPPI | RGGWCCY | 1 cut(s) | 17 |
| PstI | CTGCAG | 1 cut(s) | 217 |
| PstNI | CAGNNNCTG | 3 cut(s) | 20, 212, 1238 |
| PsuI | RGATCY | 4 cut(s) | 616, 820, 1129, 1337 |
| PsyI | GACNNNGTC | 1 cut(s) | 655 |
| PvuII | CAGCTG | 2 cut(s) | 169, 275 |
| RsaI | GTAC | 2 cut(s) | 55, 795 |
| RsaNI | GTAC | 2 cut(s) | 54, 794 |
| RseI | CAYNNNNRTG | 1 cut(s) | 297 |
| SacI | GAGCTC | 1 cut(s) | 1212 |
| SaqAI | TTAA | 4 cut(s) | 144, 240, 1059, 1254 |
| SatI | GCNGC | 4 cut(s) | 216, 930, 1248, 1287 |
| Sau3AI | GATC | 4 cut(s) | 616, 820, 1129, 1337 |
| Sau96I | GGNCC | 1 cut(s) | 17 |
| SchI | GAGTC | 6 cut(s) | 409, 458, 485, 521, 1024, 1309 |
| ScrFI | CCNGG | 8 cut(s) | 15, 192, 310, 410, 473, 501, 509, 621 |
| SduI | GDGCHC | 1 cut(s) | 1212 |
| SfaNI | GCATC | 8 cut(s) | 112, 414, 450, 477, 513, 562, 1337, 1396 |
| SfcI | CTRYAG | 2 cut(s) | 213, 1332 |
| SfuI | TTCGAA | 1 cut(s) | 29 |
| SinI | GGWCC | 1 cut(s) | 17 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 297 |
| SmlI | CTYRAG | 1 cut(s) | 1205 |
| SmoI | CTYRAG | 1 cut(s) | 1205 |
| Sse9I | AATT | 9 cut(s) | 366, 378, 556, 783, 828, 892, 984, 1019, 1137 |
| SsiI | CCGC | 2 cut(s) | 50, 1086 |
| SstI | GAGCTC | 1 cut(s) | 1212 |
| StyD4I | CCNGG | 8 cut(s) | 13, 190, 308, 408, 471, 499, 507, 619 |
| StyI | CCWWGG | 1 cut(s) | 1353 |
| TaaI | ACNGT | 6 cut(s) | 811, 967, 1000, 1120, 1324, 1385 |
| TaiI | ACGT | 2 cut(s) | 882, 1053 |
| TaqI | TCGA | 3 cut(s) | 29, 88, 1141 |
| TasI | AATT | 9 cut(s) | 366, 378, 556, 783, 828, 892, 984, 1019, 1137 |
| TfiI | GAWTC | 2 cut(s) | 755, 767 |
| Tru1I | TTAA | 4 cut(s) | 144, 240, 1059, 1254 |
| Tru9I | TTAA | 4 cut(s) | 144, 240, 1059, 1254 |
| TscAI | CASTG | 3 cut(s) | 217, 404, 865 |
| TseFI | GTSAC | 2 cut(s) | 208, 595 |
| TseI | GCWGC | 4 cut(s) | 215, 929, 1247, 1286 |
| Tsp45I | GTSAC | 2 cut(s) | 208, 595 |
| TspDTI | ATGAA | 4 cut(s) | 90, 105, 684, 905 |
| TspRI | CASTG | 3 cut(s) | 217, 404, 865 |
| Tth111I | GACNNNGTC | 1 cut(s) | 655 |
| VpaK11BI | GGWCC | 1 cut(s) | 17 |
| XapI | RAATTY | 4 cut(s) | 378, 892, 984, 1137 |