Rh5DG030600

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001372
Physical Location & Seq
Forward (+)
2187151 .. 2188110
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG030600.1

Sequence Viewer

Length: 819 bp
ATGGGGGAAAATATATTGTTCTTTCCTTGTTCGATTACTCGGTTATGTACCCATTATAAGATCCTTCGTGCCAATCTTGGTACGTGGTTTGATGCTATTGCTATTCTTGATGACCATGCCATCAGGCGGAGTGACAAGCGATGCAGTATTGCTATTCTTGGTCCGTATCGTCACATGTTGGGGTCTCTAGTGAACCCCATCCCTTACCACAAGCTGCTACTACTTCCCAAACTATGCCTCGTCCCTCTAACCCTCTCCCACCATTCTCTTGATTACTACACTCGCACAACTACCTATATTGATGCTTGCTTCAGGACTCTTCGTGATGAGTTGGTGCTTCACATATCTAACTTCGGGGTTCCTTCGACCACTTCTCATGGTGGAGATGATCACAACATGATTATTGATGGAGAGGACCATGGAGGTTACAATGAACAGAGTCAGTTGGTTGATGATGAGGTCAGGAGCCCGAACCTTGAACTCAATCAAACTCCTGTATTTGGTGCACATGCACCAAGTGCTATCATGCTGGGAGGTGAGACTGTGTGCAAGGATCCTCAAATTCAACCTGATGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGATAAAGAACCTCGATCTCGAACTCAACGTATTTGCAGAACCTACATTCGCTGCTCGTGATCCAACCAGTATAGAAGAAGAGCCTGCACCAAAGCCAAGCGAGGTGTTCGGGGGAAAGACTGTTGCATCTGAAGGATCCATTCCTTCAGGAATGATTGAACCTCATATCTCTAGTTCTTCTGGTACTGTTAGACACTATGGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

29.91

Weight (kDa)

5.28

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 57
AciI CCGC 1 cut(s) 127
AclWI GGATC 6 cut(s) 55, 548, 561, 665, 741, 754
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 3 cut(s) 295, 741, 762
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 3 cut(s) 49, 82, 796
AfiI CCNNNNNNNGG 2 cut(s) 126, 500
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 3 cut(s) 479, 566, 770
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 2 cut(s) 189, 533
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 6 cut(s) 55, 548, 561, 665, 741, 754
ApaLI GTGCAC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 214, 662
ApoI RAATTY 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 161, 415
AsuHPI GGTGA 1 cut(s) 548
AvaII GGWCC 2 cut(s) 161, 415
BaeGI GKGCMC 1 cut(s) 508
BamHI GGATCC 2 cut(s) 553, 746
BanII GRGCYC 1 cut(s) 470
BauI CACGAG 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 2 cut(s) 201, 649
BccI CCATC 3 cut(s) 128, 206, 401
BclI TGATCA 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 189, 533
BfaI CTAG 2 cut(s) 188, 783
BisI GCNGC 2 cut(s) 215, 663
BlsI GCNGC 2 cut(s) 216, 664
Bme18I GGWCC 2 cut(s) 161, 415
BmgT120I GGNCC 2 cut(s) 161, 415
BmiI GGNNCC 4 cut(s) 360, 467, 555, 748
BmsI GCATC 4 cut(s) 82, 131, 292, 746
BsaAI YACGTR 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 500
Bse1I ACTGG 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 2 cut(s) 198, 613
BseLI CCNNNNNNNGG 2 cut(s) 126, 500
BseNI ACTGG 1 cut(s) 678
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 2 cut(s) 201, 649
BseYI CCCAGC 1 cut(s) 529
BsgI GTGCAG 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 508
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 126, 500
BsmAI GTCTC 2 cut(s) 189, 533
BsmFI GGGAC 1 cut(s) 227
Bso31I GGTCTC 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 470, 508
Bsp143I GATC 6 cut(s) 60, 388, 553, 625, 670, 746
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 1 cut(s) 127
BspLI GGNNCC 4 cut(s) 360, 467, 555, 748
BspPI GGATC 6 cut(s) 55, 548, 561, 665, 741, 754
BspQI GCTCTTC 1 cut(s) 684
BspTNI GGTCTC 1 cut(s) 189
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 6 cut(s) 60, 388, 553, 625, 670, 746
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 1 cut(s) 418
Bst2BI CACGAG 1 cut(s) 666
Bst4CI ACNGT 3 cut(s) 544, 733, 799
Bst6I CTCTTC 2 cut(s) 324, 684
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 307, 696
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 2 cut(s) 198, 613
BstKTI GATC 6 cut(s) 63, 391, 556, 628, 673, 749
BstMAI GTCTC 2 cut(s) 189, 533
BstMBI GATC 6 cut(s) 60, 388, 553, 625, 670, 746
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstNSI RCATGY 3 cut(s) 178, 512, 817
BstSLI GKGCMC 1 cut(s) 508
BstV1I GCAGC 2 cut(s) 201, 649
BstX2I RGATCY 3 cut(s) 60, 553, 746
BstYI RGATCY 3 cut(s) 60, 553, 746
BtgI CCRYGG 1 cut(s) 418
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 2 cut(s) 198, 613
Cac8I GCNNGC 2 cut(s) 307, 696
Cfr13I GGNCC 2 cut(s) 161, 415
Csp6I GTAC 3 cut(s) 48, 81, 795
CviAII CATG 8 cut(s) 116, 175, 377, 397, 419, 509, 526, 814
CviJI RGCY 4 cut(s) 214, 468, 694, 706
CviKI_1 RGCY 4 cut(s) 214, 468, 694, 706
CviQI GTAC 3 cut(s) 48, 81, 795
DpnI GATC 6 cut(s) 62, 390, 555, 627, 672, 748
DpnII GATC 6 cut(s) 60, 388, 553, 625, 670, 746
DraIII CACNNNGTG 1 cut(s) 518
Eam1104I CTCTTC 2 cut(s) 324, 684
EarI CTCTTC 2 cut(s) 324, 684
EciI GGCGGA 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 470
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 161, 415
Eco57I CTGAAG 3 cut(s) 295, 741, 762
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 8 cut(s) 119, 178, 380, 400, 422, 512, 529, 817
FaqI GGGAC 1 cut(s) 227
FatI CATG 8 cut(s) 115, 174, 376, 396, 418, 508, 525, 813
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 215, 663
FokI GGATG 2 cut(s) 185, 620
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 2 cut(s) 215, 663
FspBI CTAG 2 cut(s) 188, 783
GluI GCNGC 2 cut(s) 215, 663
GsaI CCCAGC 1 cut(s) 533
Hin1II CATG 8 cut(s) 119, 178, 380, 400, 422, 512, 529, 817
HinfI GANTC 2 cut(s) 316, 439
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 2 cut(s) 193, 506
Hpy188I TCNGA 2 cut(s) 586, 742
Hpy188III TCNNGA 9 cut(s) 107, 269, 313, 323, 463, 605, 629, 668, 759
Hpy8I GTNNAC 2 cut(s) 193, 506
HpyAV CCTTC 4 cut(s) 74, 372, 737, 765
HpyCH4III ACNGT 3 cut(s) 544, 733, 799
HpyCH4IV ACGT 2 cut(s) 83, 639
HpyCH4V TGCA 8 cut(s) 144, 506, 512, 549, 596, 647, 698, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
HpySE526I ACGT 2 cut(s) 83, 639
Hsp92II CATG 8 cut(s) 119, 178, 380, 400, 422, 512, 529, 817
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 6 cut(s) 60, 388, 553, 625, 670, 746
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 2 cut(s) 201, 649
LweI GCATC 4 cut(s) 82, 131, 292, 746
MaeI CTAG 2 cut(s) 188, 783
MaeII ACGT 2 cut(s) 83, 639
MaeIII GTNAC 3 cut(s) 131, 170, 425
MalI GATC 6 cut(s) 62, 390, 555, 627, 672, 748
MboI GATC 6 cut(s) 60, 388, 553, 625, 670, 746
MboII GAAGA 4 cut(s) 311, 698, 701, 780
MflI RGATCY 3 cut(s) 60, 553, 746
MhlI GDGCHC 2 cut(s) 470, 508
MluCI AATT 1 cut(s) 561
MlyI GAGTC 2 cut(s) 310, 448
MmeI TCCRAC 1 cut(s) 698
MwoI GCNNNNNNNGC 1 cut(s) 518
NcoI CCATGG 1 cut(s) 418
NdeII GATC 6 cut(s) 60, 388, 553, 625, 670, 746
NlaIII CATG 8 cut(s) 119, 178, 380, 400, 422, 512, 529, 817
NlaIV GGNNCC 4 cut(s) 360, 467, 555, 748
NmuCI GTSAC 2 cut(s) 131, 170
NspI RCATGY 3 cut(s) 178, 512, 817
PciI ACATGT 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PkrI GCNGC 2 cut(s) 216, 664
PleI GAGTC 2 cut(s) 310, 447
PpsI GAGTC 2 cut(s) 310, 447
Ppu21I YACGTR 1 cut(s) 84
PscI ACATGT 1 cut(s) 174
PsiI TTATAA 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 529
PspN4I GGNNCC 4 cut(s) 360, 467, 555, 748
PspPI GGNCC 2 cut(s) 161, 415
PsuI RGATCY 3 cut(s) 60, 553, 746
RsaI GTAC 3 cut(s) 49, 82, 796
RsaNI GTAC 3 cut(s) 48, 81, 795
SapI GCTCTTC 1 cut(s) 684
SatI GCNGC 2 cut(s) 215, 663
Sau3AI GATC 6 cut(s) 60, 388, 553, 625, 670, 746
Sau96I GGNCC 2 cut(s) 161, 415
SchI GAGTC 2 cut(s) 310, 448
SduI GDGCHC 2 cut(s) 470, 508
SfaNI GCATC 4 cut(s) 82, 131, 292, 746
SinI GGWCC 2 cut(s) 161, 415
Sse9I AATT 1 cut(s) 561
SsiI CCGC 1 cut(s) 127
SspMI CTAG 2 cut(s) 188, 783
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 544, 733, 799
TaiI ACGT 2 cut(s) 86, 642
TaqI TCGA 4 cut(s) 32, 365, 624, 630
TasI AATT 1 cut(s) 561
TseFI GTSAC 2 cut(s) 131, 170
TseI GCWGC 2 cut(s) 214, 662
Tsp45I GTSAC 2 cut(s) 131, 170
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 1 cut(s) 153
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 2 cut(s) 161, 415
XapI RAATTY 1 cut(s) 561
XceI RCATGY 3 cut(s) 178, 512, 817
XspI CTAG 2 cut(s) 188, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.