Rh5DG030200

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001372
Physical Location & Seq
Reverse (-)
2170936 .. 2172663
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG030200.1

Sequence Viewer

Length: 1377 bp
ATGAAGCGGAAACGTGGACGTCCACGTAAGGATGAGAATTTAGTTCATGGAGAGAGCATGCCTGCAATACCAGGACCTGATAGTTTGAAGAAAAACAAGCAGAGTGCGGGTTCATTTGATGCTGGCTATCTTCTGAATGTTGAGGTAGAAGACACAGACACTCAGCTGAGGGCTGTTGTATTTCTACCAGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCAACCTTCTGAGGTGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCAATATTCCTCAGTGTGGCATCCCGGAGTCTCTTAGAAGTCAATCTTTCTCTGGCATCCCGGAGTCTCTTAGAAGCCAACCAACCTCTTTTGTAATTCCTCCGGTTGATAATTTGCCAAAAGATGATGCCCCTACCTTAGGAGGTAAAGTCATACCACAGCAACAGTTGGAGCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAATTGTTGGAGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAAACAGGAACATCATCTGAACCAATTGGTGAGGTACTAAGCATAGACACAGTACGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCCGTGCATTGGTTCAGGATAACGTAAAGAGCCTGAATTTTGAAGTCAACCAAACTCCTGTATTTGCTGAAGCTGCTCCTGCAATGGCTATCATACCAAGCGTTGAAACTGGTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCTACACCAAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCAGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTAGATCTTGAACTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTCACTGCCAGTAAACCAGTTGAGAATCTGATGGAAGAAGAGGCTGCACCAAAGCCGAGTCAGGTGTTAGGGGGAGGTACTGTTGCATCTATAGGATCCATTCCTTCAGCCATGGGTGAACCTCCTTCTGGTGCTGTTGACAGTATGGAATGTGATATTGCAGATGCCATTCCACCAGCACAATCTTGGATCAGCTCCGTAGTACCAGGCTGGTTGGGCCGGTTTTTTCATTTCAGTCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

458

Amino Acids

49.06

Weight (kDa)

4.63

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
Acc36I ACCTGC 3 cut(s) 288, 367, 1135
AciI CCGC 2 cut(s) 7, 107
AclI AACGTT 1 cut(s) 939
AclWI GGATC 7 cut(s) 752, 765, 1013, 1026, 1221, 1234, 1328
AcoI YGGCCR 2 cut(s) 308, 648
AcsI RAATTY 4 cut(s) 37, 375, 829, 1026
AcuI CTGAAG 2 cut(s) 882, 1221
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 4 cut(s) 732, 750, 1210, 1335
AfiI CCNNNNNNNGG 4 cut(s) 398, 521, 802, 1259
AgsI TTSAA 9 cut(s) 88, 617, 644, 770, 836, 899, 944, 1031, 1097
AjnI CCWGG 4 cut(s) 70, 187, 556, 1336
AluBI AGCT 4 cut(s) 166, 272, 866, 1326
AluI AGCT 4 cut(s) 166, 272, 866, 1326
Alw26I GTCTC 3 cut(s) 417, 453, 998
AlwI GGATC 7 cut(s) 752, 765, 1013, 1026, 1221, 1234, 1328
AlwNI CAGNNNCTG 3 cut(s) 77, 209, 1127
AoxI GGCC 3 cut(s) 308, 648, 1348
ApeKI GCWGC 3 cut(s) 212, 866, 1175
ApoI RAATTY 4 cut(s) 37, 375, 829, 1026
AspS9I GGNCC 2 cut(s) 74, 1348
AsuC2I CCSGG 3 cut(s) 307, 407, 443
AsuHPI GGTGA 3 cut(s) 355, 737, 1259
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 2 cut(s) 520, 774
BamHI GGATCC 3 cut(s) 757, 1018, 1226
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
BbsI GAAGAC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 224, 853, 1162
BccI CCATC 2 cut(s) 319, 1156
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 4 cut(s) 72, 189, 558, 1338
BcnI CCSGG 3 cut(s) 307, 407, 443
BcoDI GTCTC 3 cut(s) 417, 453, 998
BfaI CTAG 1 cut(s) 1088
BfmI CTRYAG 3 cut(s) 210, 360, 1221
BfuAI ACCTGC 3 cut(s) 288, 367, 1135
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 3 cut(s) 213, 867, 1176
BlsI GCNGC 3 cut(s) 214, 868, 1177
Bme1390I CCNGG 7 cut(s) 72, 189, 307, 407, 443, 558, 1338
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 74, 1348
BmiI GGNNCC 4 cut(s) 555, 759, 1020, 1228
BmrFI CCNGG 7 cut(s) 72, 189, 307, 407, 443, 558, 1338
BmrI ACTGGG 1 cut(s) 1004
BmsI GCATC 6 cut(s) 109, 411, 447, 499, 1226, 1285
BmuI ACTGGG 1 cut(s) 1004
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 167
BpuMI CCSGG 3 cut(s) 307, 407, 443
BsaAI YACGTR 2 cut(s) 26, 752
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 1242
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 1197, 1227
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 521, 802, 1259
Bse118I RCCGGY 2 cut(s) 646, 1350
Bse1I ACTGG 6 cut(s) 203, 676, 907, 999, 1140, 1148
Bse21I CCTNAGG 2 cut(s) 520, 774
Bse3DI GCAATG 1 cut(s) 882
BseBI CCWGG 4 cut(s) 72, 189, 558, 1338
BseDI CCNNGG 1 cut(s) 1242
BseGI GGATG 5 cut(s) 37, 402, 438, 1045, 1078
BseLI CCNNNNNNNGG 4 cut(s) 398, 521, 802, 1259
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 5 cut(s) 158, 176, 330, 407, 788
BseNI ACTGG 6 cut(s) 203, 676, 907, 999, 1140, 1148
BseRI GAGGAG 1 cut(s) 1101
BseXI GCAGC 3 cut(s) 224, 853, 1162
BsgI GTGCAG 1 cut(s) 1161
BshFI GGCC 3 cut(s) 310, 650, 1350
BsiSI CCGG 6 cut(s) 307, 407, 443, 485, 647, 1351
BslI CCNNNNNNNGG 4 cut(s) 398, 521, 802, 1259
BsmAI GTCTC 3 cut(s) 417, 453, 998
BsmI GAATGC 1 cut(s) 1130
BsnI GGCC 3 cut(s) 310, 650, 1350
Bsp143I GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
Bsp19I CCATGG 1 cut(s) 1242
BspACI CCGC 2 cut(s) 7, 107
BspANI GGCC 3 cut(s) 310, 650, 1350
BspCNI CTCAG 5 cut(s) 159, 175, 331, 406, 787
BspLI GGNNCC 4 cut(s) 555, 759, 1020, 1228
BspMAI CTGCAG 2 cut(s) 214, 364
BspMI ACCTGC 3 cut(s) 288, 367, 1135
BspPI GGATC 7 cut(s) 752, 765, 1013, 1026, 1221, 1234, 1328
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 2 cut(s) 646, 1350
BsrI ACTGG 6 cut(s) 203, 676, 907, 999, 1140, 1148
BssAI RCCGGY 2 cut(s) 646, 1350
BssECI CCNNGG 1 cut(s) 1242
BssMI GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 1242
Bst2UI CCWGG 4 cut(s) 72, 189, 558, 1338
Bst4CI ACNGT 5 cut(s) 549, 748, 1009, 1213, 1274
Bst6I CTCTTC 1 cut(s) 1164
BstACI GRCGYC 1 cut(s) 19
BstAPI GCANNNNNTGC 2 cut(s) 1127, 1136
BstBAI YACGTR 2 cut(s) 26, 752
BstC8I GCNNGC 3 cut(s) 59, 63, 124
BstDEI CTNAG 9 cut(s) 162, 167, 339, 393, 416, 452, 520, 734, 774
BstDSI CCRYGG 1 cut(s) 1242
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 5 cut(s) 37, 402, 438, 1045, 1078
BstKTI GATC 5 cut(s) 760, 1021, 1093, 1229, 1323
BstMAI GTCTC 3 cut(s) 417, 453, 998
BstMBI GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
BstMWI GCNNNNNNNGC 6 cut(s) 316, 866, 872, 1127, 1136, 1347
BstNI CCWGG 4 cut(s) 72, 189, 558, 1338
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 7 cut(s) 70, 187, 305, 405, 441, 556, 1336
BstSFI CTRYAG 3 cut(s) 210, 360, 1221
BstSNI TACGTA 1 cut(s) 752
BstV1I GCAGC 3 cut(s) 224, 853, 1162
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 4 cut(s) 757, 1018, 1090, 1226
BstYI RGATCY 4 cut(s) 757, 1018, 1090, 1226
Bsu36I CCTNAGG 2 cut(s) 520, 774
BsuRI GGCC 3 cut(s) 310, 650, 1350
BtgI CCRYGG 1 cut(s) 1242
BtsCI GGATG 5 cut(s) 37, 402, 438, 1045, 1078
BtsI GCAGTG 2 cut(s) 207, 1134
BtsIMutI CAGTG 3 cut(s) 207, 401, 1134
BveI ACCTGC 3 cut(s) 288, 367, 1135
Cac8I GCNNGC 3 cut(s) 59, 63, 124
CaiI CAGNNNCTG 3 cut(s) 77, 209, 1127
Cfr10I RCCGGY 2 cut(s) 646, 1350
Cfr13I GGNCC 2 cut(s) 74, 1348
Csp6I GTAC 4 cut(s) 731, 749, 1209, 1334
CviAII CATG 4 cut(s) 47, 58, 581, 1243
CviQI GTAC 4 cut(s) 731, 749, 1209, 1334
DdeI CTNAG 9 cut(s) 162, 167, 339, 393, 416, 452, 520, 734, 774
DpnI GATC 5 cut(s) 759, 1020, 1092, 1228, 1322
DpnII GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
EaeI YGGCCR 2 cut(s) 308, 648
Eam1104I CTCTTC 1 cut(s) 1164
EarI CTCTTC 1 cut(s) 1164
Eco105I TACGTA 1 cut(s) 752
Eco130I CCWWGG 1 cut(s) 1242
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 2 cut(s) 882, 1221
Eco81I CCTNAGG 2 cut(s) 520, 774
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 4 cut(s) 70, 187, 556, 1336
EcoT14I CCWWGG 1 cut(s) 1242
ErhI CCWWGG 1 cut(s) 1242
FaeI CATG 4 cut(s) 50, 61, 584, 1246
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FatI CATG 4 cut(s) 46, 57, 580, 1242
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 213, 867, 1176
FokI GGATG 5 cut(s) 44, 389, 425, 1052, 1085
Fsp4HI GCNGC 3 cut(s) 213, 867, 1176
FspBI CTAG 1 cut(s) 1088
GluI GCNGC 3 cut(s) 213, 867, 1176
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 3 cut(s) 310, 650, 1350
HapII CCGG 6 cut(s) 307, 407, 443, 485, 647, 1351
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 4 cut(s) 50, 61, 584, 1246
HincII GTYRAC 3 cut(s) 277, 841, 1270
HindII GTYRAC 3 cut(s) 277, 841, 1270
HinfI GANTC 5 cut(s) 410, 446, 692, 1156, 1189
HpaII CCGG 6 cut(s) 307, 407, 443, 485, 647, 1351
HphI GGTGA 3 cut(s) 355, 737, 1259
Hpy166II GTNNAC 9 cut(s) 17, 23, 198, 277, 841, 937, 1145, 1250, 1270
Hpy188I TCNGA 8 cut(s) 135, 316, 340, 598, 715, 790, 1051, 1161
Hpy188III TCNNGA 4 cut(s) 368, 809, 1070, 1094
Hpy8I GTNNAC 9 cut(s) 17, 23, 198, 277, 841, 937, 1145, 1250, 1270
HpyAV CCTTC 3 cut(s) 345, 1245, 1266
HpyCH4III ACNGT 5 cut(s) 549, 748, 1009, 1213, 1274
HpyCH4IV ACGT 6 cut(s) 13, 19, 25, 751, 816, 939
HpyF10VI GCNNNNNNNGC 6 cut(s) 316, 866, 872, 1127, 1136, 1347
HpyF3I CTNAG 9 cut(s) 162, 167, 339, 393, 416, 452, 520, 734, 774
HpySE526I ACGT 6 cut(s) 13, 19, 25, 751, 816, 939
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 4 cut(s) 50, 61, 584, 1246
Kzo9I GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
LmnI GCTCC 4 cut(s) 232, 553, 874, 1331
Lsp1109I GCAGC 3 cut(s) 224, 853, 1162
LweI GCATC 6 cut(s) 109, 411, 447, 499, 1226, 1285
MaeI CTAG 1 cut(s) 1088
MaeII ACGT 6 cut(s) 13, 19, 25, 751, 816, 939
MaeIII GTNAC 3 cut(s) 205, 323, 1367
MalI GATC 5 cut(s) 759, 1020, 1092, 1228, 1322
MboI GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
MboII GAAGA 5 cut(s) 100, 122, 161, 1178, 1181
MfeI CAATTG 2 cut(s) 720, 908
MflI RGATCY 4 cut(s) 757, 1018, 1090, 1226
MlyI GAGTC 3 cut(s) 419, 455, 1198
MmeI TCCRAC 2 cut(s) 531, 612
MseI TTAA 2 cut(s) 237, 1375
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 2 cut(s) 166, 272
MspI CCGG 6 cut(s) 307, 407, 443, 485, 647, 1351
MspR9I CCNGG 7 cut(s) 72, 189, 307, 407, 443, 558, 1338
MunI CAATTG 2 cut(s) 720, 908
Mva1269I GAATGC 1 cut(s) 1130
MvaI CCWGG 4 cut(s) 72, 189, 558, 1338
MwoI GCNNNNNNNGC 6 cut(s) 316, 866, 872, 1127, 1136, 1347
NciI CCSGG 3 cut(s) 307, 407, 443
NcoI CCATGG 1 cut(s) 1242
NdeII GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
NlaIII CATG 4 cut(s) 50, 61, 584, 1246
NlaIV GGNNCC 4 cut(s) 555, 759, 1020, 1228
NmeAIII GCCGAG 1 cut(s) 1212
NmuCI GTSAC 2 cut(s) 205, 1367
NspI RCATGY 1 cut(s) 61
PaeI GCATGC 1 cut(s) 61
PctI GAATGC 1 cut(s) 1130
PfeI GAWTC 2 cut(s) 692, 1156
PflFI GACNNNGTC 1 cut(s) 592
PfoI TCCNGGA 2 cut(s) 405, 441
PkrI GCNGC 3 cut(s) 214, 868, 1177
PleI GAGTC 3 cut(s) 418, 454, 1197
PpsI GAGTC 3 cut(s) 418, 454, 1197
Ppu21I YACGTR 2 cut(s) 26, 752
PpuMI RGGWCCY 1 cut(s) 74
Psp1406I AACGTT 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 74
Psp6I CCWGG 4 cut(s) 70, 187, 556, 1336
PspGI CCWGG 4 cut(s) 70, 187, 556, 1336
PspN4I GGNNCC 4 cut(s) 555, 759, 1020, 1228
PspPI GGNCC 2 cut(s) 74, 1348
PspPPI RGGWCCY 1 cut(s) 74
PstI CTGCAG 2 cut(s) 214, 364
PstNI CAGNNNCTG 3 cut(s) 77, 209, 1127
PsuI RGATCY 4 cut(s) 757, 1018, 1090, 1226
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 2 cut(s) 166, 272
RsaI GTAC 4 cut(s) 732, 750, 1210, 1335
RsaNI GTAC 4 cut(s) 731, 749, 1209, 1334
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 2 cut(s) 237, 1375
SatI GCNGC 3 cut(s) 213, 867, 1176
Sau3AI GATC 5 cut(s) 757, 1018, 1090, 1226, 1320
Sau96I GGNCC 2 cut(s) 74, 1348
SchI GAGTC 3 cut(s) 419, 455, 1198
ScrFI CCNGG 7 cut(s) 72, 189, 307, 407, 443, 558, 1338
SfaNI GCATC 6 cut(s) 109, 411, 447, 499, 1226, 1285
SfcI CTRYAG 3 cut(s) 210, 360, 1221
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 294
SnaBI TACGTA 1 cut(s) 752
SphI GCATGC 1 cut(s) 61
SsiI CCGC 2 cut(s) 7, 107
SspI AATATT 1 cut(s) 388
SspMI CTAG 1 cut(s) 1088
StyD4I CCNGG 7 cut(s) 70, 187, 305, 405, 441, 556, 1336
StyI CCWWGG 1 cut(s) 1242
TaaI ACNGT 5 cut(s) 549, 748, 1009, 1213, 1274
TaiI ACGT 6 cut(s) 16, 22, 28, 754, 819, 942
TfiI GAWTC 2 cut(s) 692, 1156
Tru1I TTAA 2 cut(s) 237, 1375
Tru9I TTAA 2 cut(s) 237, 1375
TscAI CASTG 3 cut(s) 214, 401, 1141
TseFI GTSAC 2 cut(s) 205, 1367
TseI GCWGC 3 cut(s) 212, 866, 1175
Tsp45I GTSAC 2 cut(s) 205, 1367
TspDTI ATGAA 5 cut(s) 17, 35, 102, 621, 1349
TspGWI ACGGA 1 cut(s) 1318
TspRI CASTG 3 cut(s) 214, 401, 1141
Tth111I GACNNNGTC 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 74
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 4 cut(s) 37, 375, 829, 1026
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 1314
XspI CTAG 1 cut(s) 1088
ZraI GACGTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.