FvH4_3g44310

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000518
Physical Location & Seq
Reverse (-)
36950611 .. 36952093
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44310.t1

Sequence Viewer

Length: 651 bp
ATGAGAGATCTTGCAGAAGATATCATTTTCAGTAGAGGGTCAAATCTCAGTCGCTTGAACTTTCAAGGGTTCTATACCTCGCTATTGGAGAGAAATGATGAGCTAATCACTGCAGATACTGTAAGGATTCATGGAGAGAAGGTGACTGAAGTACCTCTTGTTGCTACCAGGTTTCAATATCGTCGACAAGGAATGTGTCGTGTGTTGATAAATTTACTTGAAAAGATGCTTATGGATTTAGGTGTGGAGAGATTGGTTTTGCCTGCTGTCCCCAGTGTGTTAAACACATGGACTACTGCATTTGGGTTTTCAAGAATGACAAAATCTGAGAGGCTGCAATTTCTGGATCATACGTTCCTGGACTTTCAAGATACAATAATGTGTCAAAAACTTCTAATGAAGATCTCTGCAGCCGAACCAAGCCTGTTAATAGGAACAAAGCCTCAGATGTCTAGAAGTGCTGATATTGTCGATCTTGATGAGTCCAGTGCTGCCTCTGAAGTGTGTCAACCGGAACGAACAGAGGACAGCCAAACCGTATCCCAGGGACTTGAGTATGTCTCACTTCTTCCGACTATAAATTTAGATGGAAATGAACACAAGAACCCTCATGAAATGAACACAAAATTCACTAGTACTGTAGTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

217

Amino Acids

24.48

Weight (kDa)

5.1

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IDM1_C PF23209 2 - 130 2.9e-51 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000518)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21681 FvH4_3g29252 FvH4_3g38950 FvH4_3g44310 FvH4_5g29400
malus_domestica MD03G1066300.v1.1
rosa_chinensis RchiOBHm_Chr2g0087341 RchiOBHm_Chr7g0225691 RchiOBHm_Chr7g0225721 RchiOBHm_Chr7g0225751 RchiOBHm_Chr7g0225781 RchiOBHm_Chr7g0225811 RchiOBHm_Chr7g0225821 RchiOBHm_Chr7g0225841 RchiOBHm_Chr7g0225941
rosa_laevigata RLG00000001777 RLG00000001779 RLG00000001787 RLG00000001796 RLG00000015858 RLG00000018399 RLG00000030202
rosa_multiflora Rmu_co7995100.1_g000001 Rmu_sc0003367.1_g000025 Rmu_sc0004082.1_g000018 Rmu_sc0006064.1_g000002 Rmu_sc0011806.1_g000001 Rmu_sc0014642.1_g000005 Rmu_sc0014767.1_g000003 Rmu_sc0015723.1_g000001 Rmu_sc0030795.1_g000001 Rmu_sc0033647.1_g000001
rosa_roxburghii Rroxscaffold_1G00028220 Rroxscaffold_2G00089180 Rroxscaffold_2G00125860 Rroxscaffold_3G00233350 Rroxscaffold_3G00239570 Rroxscaffold_4G00325690 Rroxscaffold_5G00348560 Rroxscaffold_7G00198070
rosa_rugosa Rorug01G0045600 Rorug01G0474300.1 Rorug07G0220000 Rorug07G0220100 Rorug07G0220200 Rorug07G0220300 Rorug07G0232800 Rorug07G0233000 Rorug07G0233100 Rorug07G0233100 Rorug07G0233200 Rorug07G0233300.1
rosa_samantha Rh1AG061100 Rh1BG052200 Rh1CG063300 Rh1CG063600 Rh1DG067700 Rh2AG027900 Rh2BG027400 Rh2CG028200 Rh2DG028000 Rh7AG369700 Rh7AG370100 Rh7AG371200 Rh7AG387800 Rh7BG366500 Rh7BG366800 Rh7BG367000 Rh7BG367600 Rh7CG388600 Rh7CG389000 Rh7CG389800 Rh7CG407400 Rh7DG377400 Rh7DG377500 Rh7DG377900 Rh7DG378600 Rh7DG378700 Rh7DG378800
rosa_wichuraiana Rw0G020970 Rw0G020980 Rw1G005200 Rw2G002210 Rw7G031830 Rw7G031860 Rw7G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 184
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 3 cut(s) 211, 580, 626
AcuI CTGAAG 2 cut(s) 168, 519
AfaI GTAC 2 cut(s) 153, 637
AgsI TTSAA 6 cut(s) 58, 65, 176, 221, 312, 368
AhlI ACTAGT 1 cut(s) 632
AjnI CCWGG 3 cut(s) 167, 357, 543
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 1 cut(s) 354
AlwNI CAGNNNCTG 1 cut(s) 119
ApeKI GCWGC 3 cut(s) 334, 410, 491
ApoI RAATTY 3 cut(s) 211, 580, 626
AsuHPI GGTGA 1 cut(s) 154
BaeI ACNNNNGTAYC 2 cut(s) 135, 168
BbvI GCAGC 3 cut(s) 321, 422, 478
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 3 cut(s) 169, 359, 545
BciVI GTATCC 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 565
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 2 cut(s) 453, 633
BfmI CTRYAG 3 cut(s) 111, 408, 639
BfuI GTATCC 1 cut(s) 550
BglII AGATCT 2 cut(s) 7, 402
BisI GCNGC 3 cut(s) 335, 411, 492
BlsI GCNGC 3 cut(s) 336, 412, 493
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 3 cut(s) 169, 359, 545
BmrFI CCNGG 3 cut(s) 169, 359, 545
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 545, 577
BpuEI CTTGAG 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 543, 544
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bse1I ACTGG 2 cut(s) 273, 486
BseBI CCWGG 3 cut(s) 169, 359, 545
BseDI CCNNGG 2 cut(s) 543, 544
BseMII CTCAG 3 cut(s) 61, 318, 458
BseNI ACTGG 2 cut(s) 273, 486
BseXI GCAGC 3 cut(s) 321, 422, 478
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 254, 561
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 2 cut(s) 254, 561
Bsp143I GATC 4 cut(s) 7, 346, 402, 472
BspCNI CTCAG 3 cut(s) 60, 319, 457
BspHI TCATGA 1 cut(s) 610
BspMAI CTGCAG 2 cut(s) 115, 412
BspPI GGATC 1 cut(s) 354
BsrI ACTGG 2 cut(s) 273, 486
BssECI CCNNGG 2 cut(s) 543, 544
BssMI GATC 4 cut(s) 7, 346, 402, 472
Bst2UI CCWGG 3 cut(s) 169, 359, 545
Bst4CI ACNGT 3 cut(s) 121, 538, 640
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 47, 327, 444
BstKTI GATC 4 cut(s) 10, 349, 405, 475
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 4 cut(s) 7, 346, 402, 472
BstNI CCWGG 3 cut(s) 169, 359, 545
BstSCI CCNGG 3 cut(s) 167, 357, 543
BstSFI CTRYAG 3 cut(s) 111, 408, 639
BstV1I GCAGC 3 cut(s) 321, 422, 478
BstX2I RGATCY 2 cut(s) 7, 402
BstYI RGATCY 2 cut(s) 7, 402
BsuI GTATCC 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 3 cut(s) 108, 280, 493
Cac8I GCNNGC 1 cut(s) 264
CaiI CAGNNNCTG 1 cut(s) 119
CciI TCATGA 1 cut(s) 610
CsiI ACCWGGT 1 cut(s) 167
Csp6I GTAC 2 cut(s) 152, 636
CviAII CATG 3 cut(s) 131, 288, 611
CviJI RGCY 6 cut(s) 103, 334, 413, 423, 442, 531
CviKI_1 RGCY 6 cut(s) 103, 334, 413, 423, 442, 531
CviQI GTAC 2 cut(s) 152, 636
DdeI CTNAG 3 cut(s) 47, 327, 444
DpnI GATC 4 cut(s) 9, 348, 404, 474
DpnII GATC 4 cut(s) 7, 346, 402, 472
Eco32I GATATC 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 168, 519
EcoRII CCWGG 3 cut(s) 167, 357, 543
EcoRV GATATC 1 cut(s) 22
FaeI CATG 3 cut(s) 134, 291, 614
FaiI YATR 8 cut(s) 75, 132, 233, 289, 351, 558, 578, 612
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 2 cut(s) 254, 561
FatI CATG 3 cut(s) 130, 287, 610
FblI GTMKAC 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 335, 411, 492
Fsp4HI GCNGC 3 cut(s) 335, 411, 492
FspBI CTAG 2 cut(s) 453, 633
GluI GCNGC 3 cut(s) 335, 411, 492
HapII CCGG 1 cut(s) 512
Hin1II CATG 3 cut(s) 134, 291, 614
HincII GTYRAC 2 cut(s) 185, 509
HindII GTYRAC 2 cut(s) 185, 509
HinfI GANTC 2 cut(s) 127, 482
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 185, 509
Hpy188I TCNGA 4 cut(s) 328, 447, 499, 573
Hpy188III TCNNGA 6 cut(s) 312, 344, 368, 453, 476, 611
Hpy8I GTNNAC 2 cut(s) 185, 509
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 3 cut(s) 121, 538, 640
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 5 cut(s) 14, 113, 299, 337, 410
HpyF3I CTNAG 3 cut(s) 47, 327, 444
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 3 cut(s) 134, 291, 614
Kzo9I GATC 4 cut(s) 7, 346, 402, 472
Lsp1109I GCAGC 3 cut(s) 321, 422, 478
LweI GCATC 1 cut(s) 216
MabI ACCWGGT 1 cut(s) 167
MaeI CTAG 2 cut(s) 453, 633
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 142
MalI GATC 4 cut(s) 9, 348, 404, 474
MboI GATC 4 cut(s) 7, 346, 402, 472
MboII GAAGA 3 cut(s) 29, 412, 560
MflI RGATCY 2 cut(s) 7, 402
MluCI AATT 4 cut(s) 211, 338, 580, 626
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 8 cut(s) 29, 88, 165, 324, 453, 505, 517, 618
MseI TTAA 2 cut(s) 281, 428
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 3 cut(s) 169, 359, 545
MvaI CCWGG 3 cut(s) 169, 359, 545
NdeII GATC 4 cut(s) 7, 346, 402, 472
NlaIII CATG 3 cut(s) 134, 291, 614
NmuCI GTSAC 1 cut(s) 142
PagI TCATGA 1 cut(s) 610
PasI CCCWGGG 1 cut(s) 544
PfeI GAWTC 1 cut(s) 127
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 3 cut(s) 336, 412, 493
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
Psp6I CCWGG 3 cut(s) 167, 357, 543
PspGI CCWGG 3 cut(s) 167, 357, 543
PstI CTGCAG 2 cut(s) 115, 412
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 2 cut(s) 7, 402
RsaI GTAC 2 cut(s) 153, 637
RsaNI GTAC 2 cut(s) 152, 636
SalI GTCGAC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 281, 428
SatI GCNGC 3 cut(s) 335, 411, 492
Sau3AI GATC 4 cut(s) 7, 346, 402, 472
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 3 cut(s) 169, 359, 545
SetI ASST 7 cut(s) 80, 105, 144, 157, 173, 244, 356
SexAI ACCWGGT 1 cut(s) 167
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 3 cut(s) 111, 408, 639
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 4 cut(s) 211, 338, 580, 626
SspMI CTAG 2 cut(s) 453, 633
StyD4I CCNGG 3 cut(s) 167, 357, 543
TaaI ACNGT 3 cut(s) 121, 538, 640
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 184, 471
TasI AATT 4 cut(s) 211, 338, 580, 626
TatI WGTACW 1 cut(s) 635
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 281, 428
Tru9I TTAA 2 cut(s) 281, 428
TscAI CASTG 3 cut(s) 115, 280, 493
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 3 cut(s) 334, 410, 491
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 5 cut(s) 119, 413, 609, 627, 632
TspRI CASTG 3 cut(s) 115, 280, 493
XapI RAATTY 3 cut(s) 211, 580, 626
XbaI TCTAGA 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 184
XspI CTAG 2 cut(s) 453, 633
ZrmI AGTACT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.