Rh2BG027400

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000518
Physical Location & Seq
Reverse (-)
1846341 .. 1847556
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG027400.1

Sequence Viewer

Length: 1047 bp
ATGCCGAAGCGAAAGGGAGAGTCGTCTGACTCGTCCATGTCTATGGGTTCTTCACTTCCGATCTCGGAAGCGATTAGGGTTCGGGCCTCGAGCGAGATCGGCCGTGGCAGCAAGACCAGAGATTCCGATCTTGTTCGAGAAAATGCAAGAAACCATCTTTTGGCGACGGGCTGGACTTTCTTGCGGGCAAGAAAGTCCAACGATTACATGGAGCTCCGGTACCACTCACCGGCCGGAAAAGTCTTCTACTCACTCCGTACAGCCTTCAAGAGCGTAGTTTCTCAAAGCCTCGAAGTGGACTCAACGACGTCGTCGCCTTTGTTCTGCATGAAGACTTGTTCATCAAGAAAGAGAAGGAAGATAAAGATGAGAACAATGGAGGACGTAATGAACGACAAAAGGTTTTTTCTCTCCGGAATTGACAGGCTTTTGGGAAGGCCAAGTCCGGTGAACGGCCGGAGCGATCTTTCTTGGACTTTACTAAAGTCCGACTTGAAAGAGAACAAGATTGACAACCTCAAGCTCAACCAAGCCCTAAAAGTCATGCACCAGTGTTTTAAGCCTTCTAAGGACCCTTTGACCCACAAAGATGTAGCGGAGGACATCATTTTCAATAGGGAAAGAGAGTTTTACACAGTCGTTTTGGAGAAGCAAGACAAGGTGGTCACCGCCGCTAATGTGAGGATTCATGGTGTGGTGGCAGAAGTACCACTAGTGGCAACCAGGTTTGAGTTTCGGAGACAAGGTATGTGCAAGATTTTGATGAATGAGATTGAGAACTGGCTTAGGGGCTCCGGGATCGAGAGGCTTGTCTTGCCTTCGGCACAAAGCGCGCTGAAGACATGGACTAGTAGTTCGATTGGGTTTTCGACTATGACTGAGGACGAGAAGGCAGATCTTCTCATTGCTTATGATTGCTTGGATTTCCAGGATACTGTTATGTGTGAGAAACAACTCTTGAAGAAGAATGAGAACGGTGATGGTGAGTCTAGTGATGAGGGCTCTACAATATCTCATGAAGACTACATTTATGGGGACGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

348

Amino Acids

39.48

Weight (kDa)

8.6

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 36 - 95 2.8e-16 Domain of unknown function (DUF7028)
IDM1_C PF23209 186 - 318 1.1e-37 Increased DNA methylation 1, C-terminal domain
Acetyltransf_1 PF00583 194 - 278 2.4e-06 Acetyltransferase (GNAT) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000518)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21681 FvH4_3g29252 FvH4_3g38950 FvH4_3g44310 FvH4_5g29400
malus_domestica MD03G1066300.v1.1
rosa_chinensis RchiOBHm_Chr2g0087341 RchiOBHm_Chr7g0225691 RchiOBHm_Chr7g0225721 RchiOBHm_Chr7g0225751 RchiOBHm_Chr7g0225781 RchiOBHm_Chr7g0225811 RchiOBHm_Chr7g0225821 RchiOBHm_Chr7g0225841 RchiOBHm_Chr7g0225941
rosa_laevigata RLG00000001777 RLG00000001779 RLG00000001787 RLG00000001796 RLG00000015858 RLG00000018399 RLG00000030202
rosa_multiflora Rmu_co7995100.1_g000001 Rmu_sc0003367.1_g000025 Rmu_sc0004082.1_g000018 Rmu_sc0006064.1_g000002 Rmu_sc0011806.1_g000001 Rmu_sc0014642.1_g000005 Rmu_sc0014767.1_g000003 Rmu_sc0015723.1_g000001 Rmu_sc0030795.1_g000001 Rmu_sc0033647.1_g000001
rosa_roxburghii Rroxscaffold_1G00028220 Rroxscaffold_2G00089180 Rroxscaffold_2G00125860 Rroxscaffold_3G00233350 Rroxscaffold_3G00239570 Rroxscaffold_4G00325690 Rroxscaffold_5G00348560 Rroxscaffold_7G00198070
rosa_rugosa Rorug01G0045600 Rorug01G0474300.1 Rorug07G0220000 Rorug07G0220100 Rorug07G0220200 Rorug07G0220300 Rorug07G0232800 Rorug07G0233000 Rorug07G0233100 Rorug07G0233100 Rorug07G0233200 Rorug07G0233300.1
rosa_samantha Rh1AG061100 Rh1BG052200 Rh1CG063300 Rh1CG063600 Rh1DG067700 Rh2AG027900 Rh2BG027400 Rh2CG028200 Rh2DG028000 Rh7AG369700 Rh7AG370100 Rh7AG371200 Rh7AG387800 Rh7BG366500 Rh7BG366800 Rh7BG367000 Rh7BG367600 Rh7CG388600 Rh7CG389000 Rh7CG389800 Rh7CG407400 Rh7DG377400 Rh7DG377500 Rh7DG377900 Rh7DG378600 Rh7DG378700 Rh7DG378800
rosa_wichuraiana Rw0G020970 Rw0G020980 Rw1G005200 Rw2G002210 Rw7G031830 Rw7G031860 Rw7G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AatII GACGTC 1 cut(s) 311
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 1 cut(s) 833
AccIII TCCGGA 1 cut(s) 413
AciI CCGC 4 cut(s) 184, 596, 669, 672
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 3 cut(s) 100, 231, 454
AcuI CTGAAG 1 cut(s) 857
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 3 cut(s) 221, 259, 708
AfiI CCNNNNNNNGG 4 cut(s) 160, 229, 295, 452
AgsI TTSAA 4 cut(s) 268, 496, 613, 961
AhlI ACTAGT 2 cut(s) 712, 848
AjnI CCWGG 2 cut(s) 722, 927
AloI GAACNNNNNNTCC 2 cut(s) 838, 870
AluBI AGCT 2 cut(s) 214, 523
AluI AGCT 2 cut(s) 214, 523
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 733
AlwI GGATC 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 413
AoxI GGCC 5 cut(s) 84, 100, 231, 437, 454
ApeKI GCWGC 1 cut(s) 108
ArsI GACNNNNNNTTYG 2 cut(s) 838, 870
Asp718I GGTACC 1 cut(s) 219
AspLEI GCGC 2 cut(s) 833, 835
AspS9I GGNCC 2 cut(s) 84, 571
AsuC2I CCSGG 1 cut(s) 796
AsuHPI GGTGA 5 cut(s) 219, 460, 658, 989, 995
AvaI CYCGRG 1 cut(s) 88
AvaII GGWCC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 3 cut(s) 216, 794, 1004
BbsI GAAGAC 4 cut(s) 235, 338, 845, 1026
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 120
BccI CCATC 2 cut(s) 162, 974
BceAI ACGGC 2 cut(s) 87, 469
BciT130I CCWGG 2 cut(s) 724, 929
BciVI GTATCC 1 cut(s) 925
BcnI CCSGG 1 cut(s) 796
BcoDI GTCTC 1 cut(s) 733
BcuI ACTAGT 2 cut(s) 712, 848
BfaI CTAG 3 cut(s) 713, 849, 990
BfuI GTATCC 1 cut(s) 925
BglII AGATCT 1 cut(s) 895
BisI GCNGC 2 cut(s) 109, 672
BlsI GCNGC 2 cut(s) 110, 673
Bme1390I CCNGG 3 cut(s) 724, 796, 929
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 84, 571
BmiI GGNNCC 3 cut(s) 221, 573, 793
BmrFI CCNGG 3 cut(s) 724, 796, 929
BpiI GAAGAC 4 cut(s) 235, 338, 845, 1026
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 796
BsaBI GATNNNNATC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 3 cut(s) 216, 413, 445
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 730, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 229, 295, 452
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 2 cut(s) 550, 785
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 126
BseAI TCCGGA 1 cut(s) 413
BseBI CCWGG 2 cut(s) 724, 929
BseDI CCNNGG 1 cut(s) 103
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 4 cut(s) 160, 229, 295, 452
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 1 cut(s) 870
BseNI ACTGG 2 cut(s) 550, 785
BsePI GCGCGC 1 cut(s) 831
BseX3I CGGCCG 3 cut(s) 100, 231, 454
BseXI GCAGC 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 833
Bsh1285I CGRYCG 3 cut(s) 103, 234, 457
BshFI GGCC 5 cut(s) 86, 102, 233, 439, 456
BshNI GGYRCC 1 cut(s) 219
BsiEI CGRYCG 3 cut(s) 103, 234, 457
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 7 cut(s) 217, 230, 234, 414, 446, 457, 795
BslI CCNNNNNNNGG 4 cut(s) 160, 229, 295, 452
BsmAI GTCTC 1 cut(s) 733
BsnI GGCC 5 cut(s) 86, 102, 233, 439, 456
BsoBI CYCGRG 1 cut(s) 88
Bsp1286I GDGCHC 3 cut(s) 216, 794, 1004
Bsp13I TCCGGA 1 cut(s) 413
Bsp143I GATC 6 cut(s) 60, 96, 127, 463, 798, 895
BspACI CCGC 4 cut(s) 184, 596, 669, 672
BspANI GGCC 5 cut(s) 86, 102, 233, 439, 456
BspCNI CTCAG 1 cut(s) 871
BspEI TCCGGA 1 cut(s) 413
BspFNI CGCG 1 cut(s) 833
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 3 cut(s) 221, 573, 793
BspPI GGATC 1 cut(s) 806
BspT107I GGYRCC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 2 cut(s) 550, 785
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 103
BssHII GCGCGC 1 cut(s) 831
BssMI GATC 6 cut(s) 60, 96, 127, 463, 798, 895
BssNI GRCGYC 1 cut(s) 308
Bst2UI CCWGG 2 cut(s) 724, 929
Bst4CI ACNGT 3 cut(s) 637, 937, 977
BstACI GRCGYC 1 cut(s) 308
BstC8I GCNNGC 2 cut(s) 186, 833
BstDEI CTNAG 3 cut(s) 567, 785, 879
BstDSI CCRYGG 1 cut(s) 103
BstEII GGTNACC 1 cut(s) 664
BstFNI CGCG 1 cut(s) 833
BstHHI GCGC 2 cut(s) 833, 835
BstKTI GATC 6 cut(s) 63, 99, 130, 466, 801, 898
BstMAI GTCTC 1 cut(s) 733
BstMBI GATC 6 cut(s) 60, 96, 127, 463, 798, 895
BstMCI CGRYCG 3 cut(s) 103, 234, 457
BstMWI GCNNNNNNNGC 4 cut(s) 99, 108, 814, 830
BstNI CCWGG 2 cut(s) 724, 929
BstPI GGTNACC 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 722, 794, 927
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 4 cut(s) 235, 338, 845, 1026
BstX2I RGATCY 1 cut(s) 895
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 895
BstZI CGGCCG 3 cut(s) 100, 231, 454
BsuI GTATCC 1 cut(s) 925
BsuRI GGCC 5 cut(s) 86, 102, 233, 439, 456
BtgI CCRYGG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 557
Cac8I GCNNGC 2 cut(s) 186, 833
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 2 cut(s) 833, 835
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 84, 571
CsiI ACCWGGT 1 cut(s) 722
Csp6I GTAC 3 cut(s) 220, 258, 707
CviAII CATG 7 cut(s) 37, 208, 328, 544, 689, 843, 1016
CviQI GTAC 3 cut(s) 220, 258, 707
DdeI CTNAG 3 cut(s) 567, 785, 879
DpnI GATC 6 cut(s) 62, 98, 129, 465, 800, 897
DpnII GATC 6 cut(s) 60, 96, 127, 463, 798, 895
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 3 cut(s) 100, 231, 454
EagI CGGCCG 3 cut(s) 100, 231, 454
Ecl136II GAGCTC 1 cut(s) 214
EclXI CGGCCG 3 cut(s) 100, 231, 454
Eco24I GRGCYC 3 cut(s) 216, 794, 1004
Eco47I GGWCC 1 cut(s) 571
Eco52I CGGCCG 3 cut(s) 100, 231, 454
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 857
Eco88I CYCGRG 1 cut(s) 88
Eco91I GGTNACC 1 cut(s) 664
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 571
EcoO65I GGTNACC 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 722, 927
EcoT38I GRGCYC 3 cut(s) 216, 794, 1004
FaeI CATG 7 cut(s) 40, 211, 331, 547, 692, 846, 1019
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FatI CATG 7 cut(s) 36, 207, 327, 543, 688, 842, 1015
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 109, 672
FriOI GRGCYC 3 cut(s) 216, 794, 1004
Fsp4HI GCNGC 2 cut(s) 109, 672
FspBI CTAG 3 cut(s) 713, 849, 990
GlaI GCGC 2 cut(s) 832, 834
GluI GCNGC 2 cut(s) 109, 672
HaeIII GGCC 5 cut(s) 86, 102, 233, 439, 456
HapII CCGG 7 cut(s) 217, 230, 234, 414, 446, 457, 795
HhaI GCGC 2 cut(s) 833, 835
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 7 cut(s) 40, 211, 331, 547, 692, 846, 1019
Hin6I GCGC 2 cut(s) 831, 833
HinP1I GCGC 2 cut(s) 831, 833
HinfI GANTC 6 cut(s) 20, 29, 122, 299, 685, 986
HpaII CCGG 7 cut(s) 217, 230, 234, 414, 446, 457, 795
HphI GGTGA 5 cut(s) 219, 460, 658, 989, 995
Hpy166II GTNNAC 2 cut(s) 298, 451
Hpy188I TCNGA 6 cut(s) 28, 60, 67, 127, 490, 738
Hpy188III TCNNGA 7 cut(s) 137, 268, 345, 414, 802, 958, 1016
Hpy8I GTNNAC 2 cut(s) 298, 451
Hpy99I CGWCG 4 cut(s) 169, 310, 313, 316
HpyAV CCTTC 6 cut(s) 274, 348, 429, 573, 828, 883
HpyCH4III ACNGT 3 cut(s) 637, 937, 977
HpyCH4IV ACGT 2 cut(s) 308, 384
HpyCH4V TGCA 4 cut(s) 146, 327, 547, 753
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 108, 814, 830
HpyF3I CTNAG 3 cut(s) 567, 785, 879
HpySE526I ACGT 2 cut(s) 308, 384
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 7 cut(s) 40, 211, 331, 547, 692, 846, 1019
HspAI GCGC 2 cut(s) 831, 833
Kpn2I TCCGGA 1 cut(s) 413
KpnI GGTACC 1 cut(s) 223
Kzo9I GATC 6 cut(s) 60, 96, 127, 463, 798, 895
LmnI GCTCC 4 cut(s) 211, 219, 459, 797
Lsp1109I GCAGC 1 cut(s) 120
MabI ACCWGGT 1 cut(s) 722
MaeI CTAG 3 cut(s) 713, 849, 990
MaeII ACGT 2 cut(s) 308, 384
MaeIII GTNAC 1 cut(s) 664
MalI GATC 6 cut(s) 62, 98, 129, 465, 800, 897
MboI GATC 6 cut(s) 60, 96, 127, 463, 798, 895
MboII GAAGA 9 cut(s) 42, 235, 343, 370, 850, 890, 973, 976, 1031
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 3 cut(s) 216, 794, 1004
MluCI AATT 1 cut(s) 417
MlyI GAGTC 4 cut(s) 23, 29, 293, 995
MmeI TCCRAC 2 cut(s) 222, 513
MnlI CCTC 9 cut(s) 97, 299, 373, 527, 592, 675, 798, 874, 991
MroI TCCGGA 1 cut(s) 413
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 2 cut(s) 41, 588
MspI CCGG 7 cut(s) 217, 230, 234, 414, 446, 457, 795
MspR9I CCNGG 3 cut(s) 724, 796, 929
MvaI CCWGG 2 cut(s) 724, 929
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 4 cut(s) 99, 108, 814, 830
NciI CCSGG 1 cut(s) 796
NdeII GATC 6 cut(s) 60, 96, 127, 463, 798, 895
NlaIII CATG 7 cut(s) 40, 211, 331, 547, 692, 846, 1019
NlaIV GGNNCC 3 cut(s) 221, 573, 793
NmuCI GTSAC 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 88
PagI TCATGA 1 cut(s) 1015
PauI GCGCGC 1 cut(s) 831
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 390, 459
PfeI GAWTC 2 cut(s) 122, 685
PflFI GACNNNGTC 1 cut(s) 310
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 2 cut(s) 794, 927
PkrI GCNGC 2 cut(s) 110, 673
PleI GAGTC 4 cut(s) 23, 28, 293, 994
PpsI GAGTC 4 cut(s) 23, 28, 293, 994
PpuMI RGGWCCY 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 571
Psp6I CCWGG 2 cut(s) 722, 927
PspEI GGTNACC 1 cut(s) 664
PspGI CCWGG 2 cut(s) 722, 927
PspN4I GGNNCC 3 cut(s) 221, 573, 793
PspPI GGNCC 2 cut(s) 84, 571
PspPPI RGGWCCY 1 cut(s) 571
PspXI VCTCGAGB 1 cut(s) 88
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 310
PteI GCGCGC 1 cut(s) 831
RsaI GTAC 3 cut(s) 221, 259, 708
RsaNI GTAC 3 cut(s) 220, 258, 707
RseI CAYNNNNRTG 2 cut(s) 41, 588
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 2 cut(s) 109, 672
Sau3AI GATC 6 cut(s) 60, 96, 127, 463, 798, 895
Sau96I GGNCC 2 cut(s) 84, 571
SchI GAGTC 4 cut(s) 23, 29, 293, 995
ScrFI CCNGG 3 cut(s) 724, 796, 929
SduI GDGCHC 3 cut(s) 216, 794, 1004
SetI ASST 9 cut(s) 216, 311, 387, 404, 519, 525, 663, 728, 748
SexAI ACCWGGT 1 cut(s) 722
Sfr274I CTCGAG 1 cut(s) 88
SinI GGWCC 1 cut(s) 571
SlaI CTCGAG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 41, 588
SmlI CTYRAG 2 cut(s) 88, 518
SmoI CTYRAG 2 cut(s) 88, 518
SpeI ACTAGT 2 cut(s) 712, 848
Sse9I AATT 1 cut(s) 417
SsiI CCGC 4 cut(s) 184, 596, 669, 672
SspMI CTAG 3 cut(s) 713, 849, 990
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 3 cut(s) 722, 794, 927
TaaI ACNGT 3 cut(s) 637, 937, 977
TaiI ACGT 2 cut(s) 311, 387
TaqI TCGA 6 cut(s) 89, 136, 291, 801, 857, 869
TasI AATT 1 cut(s) 417
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 2 cut(s) 122, 685
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 1 cut(s) 557
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 6 cut(s) 330, 344, 404, 677, 779, 1032
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 1 cut(s) 557
Tth111I GACNNNGTC 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 88
XspI CTAG 3 cut(s) 713, 849, 990
ZraI GACGTC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.