Rorug01G0045600

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000518
Physical Location & Seq
Reverse (-)
7723108 .. 7725355
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0045600.1

Sequence Viewer

Length: 2208 bp
ATGGCTAAGCATGGAGCTATCCTATTTTCGTATGCTTTCCCCACTCTTATACTAACCTTGAGCTTTTTCCTGTGCAAAGCCATTGATGAAAACAGAACGATCCACATTGTGTACCTAGGTTCACTTCCTGATAATGAGGTGTACTCACCAACGTCTCACCACCTTGATTTACTCCAAAAAGTTGTCGACAGCAACTCCGCTGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCCAAGCTCACTGACCGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTGGTCTCTGTCTTTCCGAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCAATGAGAAAATAAGACGGAATGCCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACTCTGGAATTTGGCCTGAATCGGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTACTTGTGAGGGCGGCCAAAATTTCACTTGCAATAAGAAGCTAATTGGAGCCCGGGTTTACAGTTCATCTTCAGAGTCTGCAAAGGATGAACAAGGCCATGGAACCCACACAGCCTCAACTGCTGCAGGGAACGCTGTAAAGGATGTGAGCTTTTATGGACTAGCAGGAGGTACTGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGTGTATAAAGTATGTGATGATCATGGGTGCCCTAGTGATGCTATATTGGCTGCTTTTGATGATGGTATTGCGGATGGAGCTGACATCATTACAGTTTCATTAGGAAGCCAGGAAGCATCTTTATTACAGTATGATCCTATTGCTATCGGTGCTTTCCATGCAATGGCAAAGGGGGTACTTACATCAAATTCCGCAGGCAACAGTGGTCCTGATGATTCAACTGTTTCAAGTGTAGCACCATGGATACTTACAGTTGCAGCAAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCGGAAATGGGACGACCATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGATCGAAGCTGGGAGCTGTTCAGACGGCTGCTTAGACAGTGGTTTAGTTAAGGGAAAAGTTGTGTTATGTGACGTGACTAACGGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTAATTTTGAGAGTTGATTATGATGATGTTTCTGAAATTGTACCCTTTCCTACAACAGCTCTTGCCAACAAAGAGCATAGTCTGATAAAGTTGTACACGAACTCCACGAAAGATCCTCGAGTAAACATACAAAGAAGTGAAGAAGAAAGGGATACTGCCGCACCTGTTGTTGCTTCATTCTCTTCACGTGGACCAAATTCAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGGGTTACTATTTTGGCTGCCTTTTCACCTATTGCTTCAGTTACAGAGAGTCTTCAAGACACAAGGCGTGTAAAATATAGTATACTATCTGGAACCTCCATGTCTTGTCCCCACGCAGCTGGTGCAGCTGCTTATGTTAAAGCATTCCATCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATTTTACTGACAACAGCAGTGCCGCTGGTGAATTTGCTTATGGATCTGGCCATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACATAAAGCTGCTATGCTGGGTCTTGGATCAGGCCAATGTTAGACTTATATCAGGCGATAACAGCACTTGCTCTACAGGATCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCTGTTGTCACACCAAACAAATCTTTTTCGTTCAAATTTCACAGAACAGTGAAGAATGTTGGGCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACATCAAAGTGGTGCCTCAAGTTCTTCCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTGATGTGACAGTTGCCGGAAAAGGTTTGCCAGATGAAGTACAATCACATGTTTCTGCATTGCTGGTGTGGTCTGATGGAATTCATAGCGTTAGAAGTCCAATTCTTATCCACAAAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

735

Amino Acids

78.37

Weight (kDa)

6.08

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 110 5.7e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 575 1.8e-44 Subtilase family
fn3_6 PF17766 630 - 731 4e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000518)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21681 FvH4_3g29252 FvH4_3g38950 FvH4_3g44310 FvH4_5g29400
malus_domestica MD03G1066300.v1.1
rosa_chinensis RchiOBHm_Chr2g0087341 RchiOBHm_Chr7g0225691 RchiOBHm_Chr7g0225721 RchiOBHm_Chr7g0225751 RchiOBHm_Chr7g0225781 RchiOBHm_Chr7g0225811 RchiOBHm_Chr7g0225821 RchiOBHm_Chr7g0225841 RchiOBHm_Chr7g0225941
rosa_laevigata RLG00000001777 RLG00000001779 RLG00000001787 RLG00000001796 RLG00000015858 RLG00000018399 RLG00000030202
rosa_multiflora Rmu_co7995100.1_g000001 Rmu_sc0003367.1_g000025 Rmu_sc0004082.1_g000018 Rmu_sc0006064.1_g000002 Rmu_sc0011806.1_g000001 Rmu_sc0014642.1_g000005 Rmu_sc0014767.1_g000003 Rmu_sc0015723.1_g000001 Rmu_sc0030795.1_g000001 Rmu_sc0033647.1_g000001
rosa_roxburghii Rroxscaffold_1G00028220 Rroxscaffold_2G00089180 Rroxscaffold_2G00125860 Rroxscaffold_3G00233350 Rroxscaffold_3G00239570 Rroxscaffold_4G00325690 Rroxscaffold_5G00348560 Rroxscaffold_7G00198070
rosa_rugosa Rorug01G0045600 Rorug01G0474300.1 Rorug07G0220000 Rorug07G0220100 Rorug07G0220200 Rorug07G0220300 Rorug07G0232800 Rorug07G0233000 Rorug07G0233100 Rorug07G0233100 Rorug07G0233200 Rorug07G0233300.1
rosa_samantha Rh1AG061100 Rh1BG052200 Rh1CG063300 Rh1CG063600 Rh1DG067700 Rh2AG027900 Rh2BG027400 Rh2CG028200 Rh2DG028000 Rh7AG369700 Rh7AG370100 Rh7AG371200 Rh7AG387800 Rh7BG366500 Rh7BG366800 Rh7BG367000 Rh7BG367600 Rh7CG388600 Rh7CG389000 Rh7CG389800 Rh7CG407400 Rh7DG377400 Rh7DG377500 Rh7DG377900 Rh7DG378600 Rh7DG378700 Rh7DG378800
rosa_wichuraiana Rw0G020970 Rw0G020980 Rw1G005200 Rw2G002210 Rw7G031830 Rw7G031860 Rw7G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 732, 2038
AccB7I CCANNNNNTGG 2 cut(s) 489, 868
AccI GTMKAC 2 cut(s) 186, 1549
AciI CCGC 6 cut(s) 198, 510, 776, 897, 1386, 1698
AclWI GGATC 9 cut(s) 94, 833, 992, 1334, 1726, 1821, 1873, 1885, 1896
AcoI YGGCCR 2 cut(s) 511, 1723
AcuI CTGAAG 3 cut(s) 552, 1017, 1488
AcvI CACGTG 1 cut(s) 1415
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 9 cut(s) 113, 143, 499, 670, 882, 973, 1269, 1322, 2124
AfiI CCNNNNNNNGG 4 cut(s) 271, 489, 868, 1026
AflIII ACRYGT 1 cut(s) 2131
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 3 cut(s) 813, 1465, 1753
Alw26I GTCTC 3 cut(s) 159, 307, 1632
AlwI GGATC 9 cut(s) 94, 833, 992, 1334, 1726, 1821, 1873, 1885, 1896
AlwNI CAGNNNCTG 2 cut(s) 575, 1868
Ama87I CYCGRG 2 cut(s) 549, 1344
AoxI GGCC 7 cut(s) 437, 511, 592, 1203, 1723, 1818, 1995
Asp700I GAANNNNTTC 1 cut(s) 1043
AspA2I CCTAGG 1 cut(s) 115
AspLEI GCGC 1 cut(s) 1463
AspS9I GGNCC 3 cut(s) 473, 911, 1418
AsuC2I CCSGG 3 cut(s) 266, 550, 551
AsuHPI GGTGA 4 cut(s) 138, 149, 1485, 1715
AvaI CYCGRG 2 cut(s) 549, 1344
AvaII GGWCC 3 cut(s) 473, 911, 1418
AvrII CCTAGG 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 1725
BanI GGYRCC 2 cut(s) 732, 2038
BanII GRGCYC 1 cut(s) 550
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 3 cut(s) 1511, 1788, 2084
BbvCI CCTCAGC 1 cut(s) 691
BccI CCATC 7 cut(s) 336, 458, 761, 773, 1623, 1646, 2153
BceAI ACGGC 1 cut(s) 1150
BciT130I CCWGG 3 cut(s) 815, 1467, 1755
BciVI GTATCC 2 cut(s) 942, 1372
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 3 cut(s) 266, 550, 551
BcoDI GTCTC 3 cut(s) 159, 307, 1632
BfaI CTAG 4 cut(s) 116, 659, 738, 1907
BfmI CTRYAG 4 cut(s) 621, 1659, 1746, 1860
BfoI RGCGCY 1 cut(s) 1464
BfuI GTATCC 2 cut(s) 942, 1372
BglII AGATCT 1 cut(s) 344
BlnI CCTAGG 1 cut(s) 115
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 6 cut(s) 266, 550, 551, 815, 1467, 1755
Bme18I GGWCC 3 cut(s) 473, 911, 1418
BmeT110I CYCGRG 2 cut(s) 549, 1344
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 3 cut(s) 473, 911, 1418
BmiI GGNNCC 6 cut(s) 547, 601, 687, 734, 1561, 2040
BmrFI CCNGG 6 cut(s) 266, 550, 551, 815, 1467, 1755
BmsI GCATC 3 cut(s) 733, 830, 1078
BpiI GAAGAC 3 cut(s) 1511, 1788, 2084
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 2 cut(s) 1236, 1614
Bpu10I CCTNAGC 1 cut(s) 691
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 3 cut(s) 79, 2004, 2028
BpuMI CCSGG 3 cut(s) 266, 550, 551
BsaAI YACGTR 1 cut(s) 1415
BsaI GGTCTC 2 cut(s) 307, 1632
BsaJI CCNNGG 9 cut(s) 115, 549, 595, 944, 1020, 1465, 1466, 1753, 1754
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 489, 868, 1026
Bse1I ACTGG 1 cut(s) 390
Bse3DI GCAATG 2 cut(s) 873, 2141
BseBI CCWGG 3 cut(s) 815, 1467, 1755
BseDI CCNNGG 9 cut(s) 115, 549, 595, 944, 1020, 1465, 1466, 1753, 1754
BseGI GGATG 5 cut(s) 328, 589, 646, 784, 1615
BseLI CCNNNNNNNGG 4 cut(s) 271, 489, 868, 1026
BseMI GCAATG 2 cut(s) 873, 2141
BseMII CTCAG 2 cut(s) 347, 705
BseNI ACTGG 1 cut(s) 390
BseRI GAGGAG 3 cut(s) 468, 693, 2087
BseSI GKGCMC 1 cut(s) 737
BseYI CCCAGC 2 cut(s) 1118, 1803
BsgI GTGCAG 1 cut(s) 1611
BshFI GGCC 7 cut(s) 439, 513, 594, 1205, 1725, 1820, 1997
BshNI GGYRCC 2 cut(s) 732, 2038
BsiHKCI CYCGRG 2 cut(s) 549, 1344
BsiSI CCGG 3 cut(s) 265, 550, 2100
BslFI GGGAC 4 cut(s) 365, 1027, 1072, 1560
BslI CCNNNNNNNGG 4 cut(s) 271, 489, 868, 1026
BsmAI GTCTC 3 cut(s) 159, 307, 1632
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 4 cut(s) 365, 1027, 1072, 1560
BsmI GAATGC 2 cut(s) 391, 1610
BsnI GGCC 7 cut(s) 439, 513, 594, 1205, 1725, 1820, 1997
Bso31I GGTCTC 2 cut(s) 307, 1632
BsoBI CYCGRG 2 cut(s) 549, 1344
Bsp1286I GDGCHC 2 cut(s) 550, 737
Bsp1407I TGTACA 1 cut(s) 1320
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 3 cut(s) 595, 944, 1020
BspACI CCGC 6 cut(s) 198, 510, 776, 897, 1386, 1698
BspANI GGCC 7 cut(s) 439, 513, 594, 1205, 1725, 1820, 1997
BspCNI CTCAG 2 cut(s) 346, 704
BspLI GGNNCC 6 cut(s) 547, 601, 687, 734, 1561, 2040
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 9 cut(s) 94, 833, 992, 1334, 1726, 1821, 1873, 1885, 1896
BspT107I GGYRCC 2 cut(s) 732, 2038
BspTNI GGTCTC 2 cut(s) 307, 1632
BsrDI GCAATG 2 cut(s) 873, 2141
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 1 cut(s) 390
BssECI CCNNGG 9 cut(s) 115, 549, 595, 944, 1020, 1465, 1466, 1753, 1754
BssNAI GTATAC 1 cut(s) 1550
BssT1I CCWWGG 4 cut(s) 115, 595, 944, 1020
Bst1107I GTATAC 1 cut(s) 1550
Bst2UI CCWGG 3 cut(s) 815, 1467, 1755
Bst4CI ACNGT 9 cut(s) 560, 799, 834, 908, 928, 958, 1148, 1960, 2095
Bst6I CTCTTC 1 cut(s) 1414
BstAPI GCANNNNNTGC 1 cut(s) 1589
BstAUI TGTACA 1 cut(s) 1320
BstBAI YACGTR 1 cut(s) 1415
BstC8I GCNNGC 3 cut(s) 282, 901, 1977
BstDEI CTNAG 5 cut(s) 6, 333, 691, 1141, 1776
BstDSI CCRYGG 3 cut(s) 595, 944, 1020
BstF5I GGATG 5 cut(s) 328, 589, 646, 784, 1615
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 1 cut(s) 1463
BstMAI GTCTC 3 cut(s) 159, 307, 1632
BstNI CCWGG 3 cut(s) 815, 1467, 1755
BstNSI RCATGY 1 cut(s) 2135
BstSCI CCNGG 6 cut(s) 264, 548, 549, 813, 1465, 1753
BstSFI CTRYAG 4 cut(s) 621, 1659, 1746, 1860
BstSLI GKGCMC 1 cut(s) 737
BstV2I GAAGAC 3 cut(s) 1511, 1788, 2084
BstX2I RGATCY 5 cut(s) 344, 1339, 1718, 1865, 1877
BstXI CCANNNNNNTGG 1 cut(s) 1586
BstYI RGATCY 5 cut(s) 344, 1339, 1718, 1865, 1877
BstZ17I GTATAC 1 cut(s) 1550
BsuI GTATCC 2 cut(s) 942, 1372
BsuRI GGCC 7 cut(s) 439, 513, 594, 1205, 1725, 1820, 1997
BtgI CCRYGG 3 cut(s) 595, 944, 1020
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 5 cut(s) 328, 589, 646, 784, 1615
BtsI GCAGTG 1 cut(s) 1699
BtsIMutI CAGTG 8 cut(s) 244, 258, 397, 913, 1041, 1153, 1699, 1965
Cac8I GCNNGC 3 cut(s) 282, 901, 1977
CaiI CAGNNNCTG 2 cut(s) 575, 1868
CfoI GCGC 1 cut(s) 1463
Cfr13I GGNCC 3 cut(s) 473, 911, 1418
Cfr9I CCCGGG 1 cut(s) 549
Csp6I GTAC 9 cut(s) 112, 142, 498, 669, 881, 972, 1268, 1321, 2123
CviQI GTAC 9 cut(s) 112, 142, 498, 669, 881, 972, 1268, 1321, 2123
DdeI CTNAG 5 cut(s) 6, 333, 691, 1141, 1776
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 511, 1723
Eam1104I CTCTTC 1 cut(s) 1414
EarI CTCTTC 1 cut(s) 1414
Eco130I CCWWGG 4 cut(s) 115, 595, 944, 1020
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 307, 1632
Eco47I GGWCC 3 cut(s) 473, 911, 1418
Eco57I CTGAAG 3 cut(s) 552, 1017, 1488
Eco72I CACGTG 1 cut(s) 1415
Eco88I CYCGRG 2 cut(s) 549, 1344
EcoRI GAATTC 2 cut(s) 321, 2163
EcoRII CCWGG 3 cut(s) 813, 1465, 1753
EcoT14I CCWWGG 4 cut(s) 115, 595, 944, 1020
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 4 cut(s) 115, 595, 944, 1020
Esp3I CGTCTC 1 cut(s) 159
FaqI GGGAC 4 cut(s) 365, 1027, 1072, 1560
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 2 cut(s) 186, 1549
FokI GGATG 5 cut(s) 315, 596, 653, 791, 1602
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 4 cut(s) 116, 659, 738, 1907
GlaI GCGC 1 cut(s) 1462
GsaI CCCAGC 2 cut(s) 1122, 1807
GsuI CTGGAG 2 cut(s) 1236, 1614
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 7 cut(s) 439, 513, 594, 1205, 1725, 1820, 1997
HapII CCGG 3 cut(s) 265, 550, 2100
HhaI GCGC 1 cut(s) 1463
Hin6I GCGC 1 cut(s) 1461
HinP1I GCGC 1 cut(s) 1461
HincII GTYRAC 2 cut(s) 187, 2026
HindII GTYRAC 2 cut(s) 187, 2026
HindIII AAGCTT 1 cut(s) 1770
HinfI GANTC 5 cut(s) 425, 443, 572, 920, 1516
HpaII CCGG 3 cut(s) 265, 550, 2100
HphI GGTGA 4 cut(s) 138, 149, 1485, 1715
Hpy188III TCNNGA 8 cut(s) 128, 211, 429, 914, 1215, 1523, 1557, 1619
HpyAV CCTTC 3 cut(s) 286, 1911, 2065
HpyCH4III ACNGT 9 cut(s) 560, 799, 834, 908, 928, 958, 1148, 1960, 2095
HpyCH4IV ACGT 3 cut(s) 152, 1182, 1414
HpyF3I CTNAG 5 cut(s) 6, 333, 691, 1141, 1776
HpySE526I ACGT 3 cut(s) 152, 1182, 1414
HspAI GCGC 1 cut(s) 1461
Ksp22I TGATCA 1 cut(s) 724
LmnI GCTCC 6 cut(s) 14, 545, 782, 1109, 1122, 1217
LweI GCATC 3 cut(s) 733, 830, 1078
MaeI CTAG 4 cut(s) 116, 659, 738, 1907
MaeII ACGT 3 cut(s) 152, 1182, 1414
MaeIII GTNAC 7 cut(s) 221, 1178, 1183, 1471, 1507, 1918, 2089
MboII GAAGA 9 cut(s) 558, 1379, 1382, 1401, 1511, 1793, 1975, 2042, 2089
MflI RGATCY 5 cut(s) 344, 1339, 1718, 1865, 1877
MhlI GDGCHC 2 cut(s) 550, 737
MlsI TGGCCA 1 cut(s) 1725
MluNI TGGCCA 1 cut(s) 1725
MlyI GAGTC 3 cut(s) 419, 581, 1525
Mox20I TGGCCA 1 cut(s) 1725
MroXI GAANNNNTTC 1 cut(s) 1043
MscI TGGCCA 1 cut(s) 1725
MseI TTAA 5 cut(s) 456, 1052, 1158, 1229, 1605
MslI CAYNNNNRTG 1 cut(s) 2033
Msp20I TGGCCA 1 cut(s) 1725
MspA1I CMGCKG 5 cut(s) 200, 1586, 1595, 1700, 1913
MspI CCGG 3 cut(s) 265, 550, 2100
MspR9I CCNGG 6 cut(s) 266, 550, 551, 815, 1467, 1755
Mva1269I GAATGC 2 cut(s) 391, 1610
MvaI CCWGG 3 cut(s) 815, 1467, 1755
NciI CCSGG 3 cut(s) 266, 550, 551
NcoI CCATGG 3 cut(s) 595, 944, 1020
NlaIV GGNNCC 6 cut(s) 547, 601, 687, 734, 1561, 2040
NmuCI GTSAC 4 cut(s) 1178, 1183, 1918, 2089
NspI RCATGY 1 cut(s) 2135
PaeR7I CTCGAG 1 cut(s) 1344
PasI CCCWGGG 2 cut(s) 1466, 1754
PceI AGGCCT 1 cut(s) 1205
PciI ACATGT 1 cut(s) 2131
PcsI WCGNNNNNNNCGW 1 cut(s) 1331
PctI GAATGC 2 cut(s) 391, 1610
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 2 cut(s) 443, 920
PflMI CCANNNNNTGG 2 cut(s) 489, 868
PleI GAGTC 3 cut(s) 419, 580, 1524
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 3 cut(s) 419, 580, 1524
Ppu21I YACGTR 1 cut(s) 1415
PscI ACATGT 1 cut(s) 2131
Psp6I CCWGG 3 cut(s) 813, 1465, 1753
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 2 cut(s) 1118, 1803
PspGI CCWGG 3 cut(s) 813, 1465, 1753
PspN4I GGNNCC 6 cut(s) 547, 601, 687, 734, 1561, 2040
PspPI GGNCC 3 cut(s) 473, 911, 1418
PspXI VCTCGAGB 1 cut(s) 1344
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 2 cut(s) 575, 1868
PsuI RGATCY 5 cut(s) 344, 1339, 1718, 1865, 1877
PvuII CAGCTG 3 cut(s) 1586, 1595, 1913
RsaI GTAC 9 cut(s) 113, 143, 499, 670, 882, 973, 1269, 1322, 2124
RsaNI GTAC 9 cut(s) 112, 142, 498, 669, 881, 972, 1268, 1321, 2123
RseI CAYNNNNRTG 1 cut(s) 2033
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 456, 1052, 1158, 1229, 1605
Sau96I GGNCC 3 cut(s) 473, 911, 1418
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 3 cut(s) 419, 581, 1525
ScrFI CCNGG 6 cut(s) 266, 550, 551, 815, 1467, 1755
SduI GDGCHC 2 cut(s) 550, 737
SfaNI GCATC 3 cut(s) 733, 830, 1078
SfcI CTRYAG 4 cut(s) 621, 1659, 1746, 1860
Sfr274I CTCGAG 1 cut(s) 1344
SinI GGWCC 3 cut(s) 473, 911, 1418
SlaI CTCGAG 1 cut(s) 1344
SmaI CCCGGG 1 cut(s) 551
SmiMI CAYNNNNRTG 1 cut(s) 2033
SmlI CTYRAG 4 cut(s) 58, 1344, 2019, 2043
SmoI CTYRAG 4 cut(s) 58, 1344, 2019, 2043
SseBI AGGCCT 1 cut(s) 1205
SsiI CCGC 6 cut(s) 198, 510, 776, 897, 1386, 1698
SspI AATATT 1 cut(s) 2004
SspMI CTAG 4 cut(s) 116, 659, 738, 1907
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 6 cut(s) 264, 548, 549, 813, 1465, 1753
StyI CCWWGG 4 cut(s) 115, 595, 944, 1020
TaaI ACNGT 9 cut(s) 560, 799, 834, 908, 928, 958, 1148, 1960, 2095
TaiI ACGT 3 cut(s) 155, 1185, 1417
TaqI TCGA 3 cut(s) 186, 1113, 1345
TatI WGTACW 4 cut(s) 141, 971, 1320, 2122
TauI GCSGC 3 cut(s) 513, 1388, 1700
TfiI GAWTC 2 cut(s) 443, 920
Tru1I TTAA 5 cut(s) 456, 1052, 1158, 1229, 1605
Tru9I TTAA 5 cut(s) 456, 1052, 1158, 1229, 1605
TscAI CASTG 8 cut(s) 244, 265, 397, 913, 1041, 1153, 1699, 1965
TseFI GTSAC 4 cut(s) 1178, 1183, 1918, 2089
Tsp45I GTSAC 4 cut(s) 1178, 1183, 1918, 2089
TspGWI ACGGA 2 cut(s) 397, 1206
TspMI CCCGGG 1 cut(s) 549
TspRI CASTG 8 cut(s) 244, 265, 397, 913, 1041, 1153, 1699, 1965
Van91I CCANNNNNTGG 2 cut(s) 489, 868
VpaK11BI GGWCC 3 cut(s) 473, 911, 1418
XceI RCATGY 1 cut(s) 2135
XhoI CTCGAG 1 cut(s) 1344
XmaI CCCGGG 1 cut(s) 549
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 2 cut(s) 186, 1549
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 4 cut(s) 116, 659, 738, 1907
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.