FvH4_5g29400

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000518
Physical Location & Seq
Reverse (-)
20372762 .. 20375459
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29400.t1

Sequence Viewer

Length: 585 bp
ATGAAGCGGAAGCGGGCATCTGCTATGGCTTCATCGCCGTCCTGGGCGACCCAAACGGCGTCGTCAAACCCCCAAATCCCATCCGGATCAAGAAAAGGATCTAAAACAAGAAAAATGAATGAGATACTAAAAGATCAAAGATCCATGATTTCTGGAATGAACCGGATTTTGGGGACACCGATTCCGGTCAGCGATAACCTGACGTGGAGGCTGTTGAAGCCTCACAAGAGAATCGAACCCTATTACGGCACCAAGCTCAGTTCCGCCCTTCATGCCATTCACGAGTGTTTCGAGCCCTCCAAGGACCCCTACACCGGCAGAGACATTTCCGAGGACATTGTTTTCGACTTGGACTCCGATTCAGAGATGAACTTGAGAGGGTTCTACACTGTGGTTTTGGAGAGGAGCGATGATGAGGAGATGATATGTGCCGCCACGCTGAGGATCAGTGGGGATGTGGCTGAACTGCCCCTTGTGGCGACTAGGCCTCAGTTTCGCAGACTTGGGATGTGCAAGATTATGATGAATGAGCTTGAAAATCGGCTTATTGAATACGGCATTGTGAGGTTGTGGACAAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

195

Amino Acids

22.0

Weight (kDa)

9.01

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IDM1_C PF23209 97 - 190 7.7e-24 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000518)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21681 FvH4_3g29252 FvH4_3g38950 FvH4_3g44310 FvH4_5g29400
malus_domestica MD03G1066300.v1.1
rosa_chinensis RchiOBHm_Chr2g0087341 RchiOBHm_Chr7g0225691 RchiOBHm_Chr7g0225721 RchiOBHm_Chr7g0225751 RchiOBHm_Chr7g0225781 RchiOBHm_Chr7g0225811 RchiOBHm_Chr7g0225821 RchiOBHm_Chr7g0225841 RchiOBHm_Chr7g0225941
rosa_laevigata RLG00000001777 RLG00000001779 RLG00000001787 RLG00000001796 RLG00000015858 RLG00000018399 RLG00000030202
rosa_multiflora Rmu_co7995100.1_g000001 Rmu_sc0003367.1_g000025 Rmu_sc0004082.1_g000018 Rmu_sc0006064.1_g000002 Rmu_sc0011806.1_g000001 Rmu_sc0014642.1_g000005 Rmu_sc0014767.1_g000003 Rmu_sc0015723.1_g000001 Rmu_sc0030795.1_g000001 Rmu_sc0033647.1_g000001
rosa_roxburghii Rroxscaffold_1G00028220 Rroxscaffold_2G00089180 Rroxscaffold_2G00125860 Rroxscaffold_3G00233350 Rroxscaffold_3G00239570 Rroxscaffold_4G00325690 Rroxscaffold_5G00348560 Rroxscaffold_7G00198070
rosa_rugosa Rorug01G0045600 Rorug01G0474300.1 Rorug07G0220000 Rorug07G0220100 Rorug07G0220200 Rorug07G0220300 Rorug07G0232800 Rorug07G0233000 Rorug07G0233100 Rorug07G0233100 Rorug07G0233200 Rorug07G0233300.1
rosa_samantha Rh1AG061100 Rh1BG052200 Rh1CG063300 Rh1CG063600 Rh1DG067700 Rh2AG027900 Rh2BG027400 Rh2CG028200 Rh2DG028000 Rh7AG369700 Rh7AG370100 Rh7AG371200 Rh7AG387800 Rh7BG366500 Rh7BG366800 Rh7BG367000 Rh7BG367600 Rh7CG388600 Rh7CG389000 Rh7CG389800 Rh7CG407400 Rh7DG377400 Rh7DG377500 Rh7DG377900 Rh7DG378600 Rh7DG378700 Rh7DG378800
rosa_wichuraiana Rw0G020970 Rw0G020980 Rw1G005200 Rw2G002210 Rw7G031830 Rw7G031860 Rw7G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 4 cut(s) 7, 13, 264, 432
AclWI GGATC 4 cut(s) 94, 106, 135, 452
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 4 cut(s) 169, 245, 314, 441
AgsI TTSAA 3 cut(s) 217, 536, 551
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 2 cut(s) 256, 532
AluI AGCT 2 cut(s) 256, 532
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 4 cut(s) 94, 106, 135, 452
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 1 cut(s) 485
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspS9I GGNCC 1 cut(s) 304
AvaII GGWCC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 440
BccI CCATC 1 cut(s) 88
BceAI ACGGC 4 cut(s) 22, 72, 262, 571
BciT130I CCWGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 1 cut(s) 483
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 1 cut(s) 43
Bme18I GGWCC 1 cut(s) 304
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 2 cut(s) 250, 306
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 42, 300, 330
BsaWI WCCGGW 3 cut(s) 83, 162, 184
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 245, 314, 441
Bse118I RCCGGY 1 cut(s) 314
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 3 cut(s) 42, 300, 330
BseGI GGATG 3 cut(s) 80, 460, 513
BseLI CCNNNNNNNGG 4 cut(s) 169, 245, 314, 441
BseMII CTCAG 3 cut(s) 271, 431, 503
BseRI GAGGAG 2 cut(s) 418, 431
BshFI GGCC 1 cut(s) 487
BshNI GGYRCC 1 cut(s) 248
BsiSI CCGG 4 cut(s) 84, 163, 185, 315
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 4 cut(s) 169, 245, 314, 441
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 5 cut(s) 86, 98, 133, 140, 444
BspACI CCGC 4 cut(s) 7, 13, 264, 432
BspANI GGCC 1 cut(s) 487
BspCNI CTCAG 3 cut(s) 270, 432, 502
BspEI TCCGGA 1 cut(s) 83
BspLI GGNNCC 2 cut(s) 250, 306
BspPI GGATC 4 cut(s) 94, 106, 135, 452
BspT107I GGYRCC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 42, 300, 330
BssMI GATC 5 cut(s) 86, 98, 133, 140, 444
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 391
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 3 cut(s) 257, 440, 489
BstF5I GGATG 3 cut(s) 80, 460, 513
BstKTI GATC 5 cut(s) 89, 101, 136, 143, 447
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 5 cut(s) 86, 98, 133, 140, 444
BstMWI GCNNNNNNNGC 2 cut(s) 217, 272
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 98, 140
BstYI RGATCY 2 cut(s) 98, 140
BsuRI GGCC 1 cut(s) 487
BtgZI GCGATG 2 cut(s) 18, 423
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 80, 460, 513
BtsIMutI CAGTG 2 cut(s) 387, 454
Cac8I GCNNGC 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 304
CseI GACGC 1 cut(s) 48
CviAII CATG 2 cut(s) 145, 272
CviJI RGCY 9 cut(s) 29, 211, 220, 256, 295, 461, 487, 532, 544
CviKI_1 RGCY 9 cut(s) 29, 211, 220, 256, 295, 461, 487, 532, 544
DdeI CTNAG 3 cut(s) 257, 440, 489
DpnI GATC 5 cut(s) 88, 100, 135, 142, 446
DpnII GATC 5 cut(s) 86, 98, 133, 140, 444
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 487
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 148, 275
FaiI YATR 5 cut(s) 26, 146, 273, 427, 521
FaqI GGGAC 1 cut(s) 187
FatI CATG 2 cut(s) 144, 271
FauI CCCGC 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 3 cut(s) 67, 467, 520
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 1 cut(s) 483
GluI GCNGC 1 cut(s) 432
HaeIII GGCC 1 cut(s) 487
HapII CCGG 4 cut(s) 84, 163, 185, 315
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 2 cut(s) 148, 275
HinfI GANTC 4 cut(s) 181, 231, 353, 359
HpaII CCGG 4 cut(s) 84, 163, 185, 315
Hpy166II GTNNAC 1 cut(s) 573
Hpy188I TCNGA 3 cut(s) 331, 358, 364
Hpy188III TCNNGA 4 cut(s) 84, 90, 153, 281
Hpy8I GTNNAC 1 cut(s) 573
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 272
HpyF3I CTNAG 3 cut(s) 257, 440, 489
HpySE526I ACGT 1 cut(s) 203
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 2 cut(s) 148, 275
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 5 cut(s) 86, 98, 133, 140, 444
LmnI GCTCC 1 cut(s) 405
LpnPI CCDG 8 cut(s) 28, 55, 97, 138, 176, 198, 212, 328
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 203
MalI GATC 5 cut(s) 88, 100, 135, 142, 446
MboI GATC 5 cut(s) 86, 98, 133, 140, 444
MflI RGATCY 2 cut(s) 98, 140
MhlI GDGCHC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 347
MroI TCCGGA 1 cut(s) 83
MspI CCGG 4 cut(s) 84, 163, 185, 315
MspR9I CCNGG 1 cut(s) 43
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 2 cut(s) 217, 272
NdeII GATC 5 cut(s) 86, 98, 133, 140, 444
NlaIII CATG 2 cut(s) 148, 275
NlaIV GGNNCC 2 cut(s) 250, 306
PceI AGGCCT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 3 cut(s) 181, 231, 359
PkrI GCNGC 1 cut(s) 433
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 250, 306
PspPI GGNCC 1 cut(s) 304
PspPPI RGGWCCY 1 cut(s) 304
PsuI RGATCY 2 cut(s) 98, 140
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 5 cut(s) 86, 98, 133, 140, 444
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 1 cut(s) 297
SetI ASST 5 cut(s) 201, 206, 258, 534, 569
SfaNI GCATC 1 cut(s) 26
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SseBI AGGCCT 1 cut(s) 487
SsiI CCGC 4 cut(s) 7, 13, 264, 432
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 1 cut(s) 487
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 206
TaqI TCGA 3 cut(s) 234, 291, 345
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 3 cut(s) 181, 231, 359
TscAI CASTG 2 cut(s) 394, 454
TspDTI ATGAA 7 cut(s) 17, 21, 131, 173, 260, 383, 539
TspRI CASTG 2 cut(s) 394, 454
VpaK11BI GGWCC 1 cut(s) 304
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.