FvH4_4g35052

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001837
Physical Location & Seq
Forward (+)
32519022 .. 32521148
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35052.t1

Sequence Viewer

Length: 639 bp
ATGCTCGTGACTCTTGGCATTACCGTGACAGAAACCGCCAAGCAGGCTAGGTGCCCACATAACATCGACGACCGTGATTTCTACATCTGCAATCCACTCACCGTGCAACTGGTTAATCTTCCTCCGACCCCTGGATGCTACGAAAGACAACCTTGGGGGTTCATCTGTGAGCCTTATTGTAAGGAAGCCAGTAATGCCGGTTTTAATGTTAATGCCGAGTATAGGTTCAAGGTTGTGAAAATTCTTGGTCCTAGTACAAGAAAAAAATGTTACAAAATTAATATCCAGGTCTTCTCTAGTGAGACTGGTGAATGGAGAGAGATAGCTGTACCATGTCCGCGAGGCTTCAAGTACTTCGACCTTAGGCGTTGGGATAGCTTTGGTAACATTGGAATTCTGTATTGGGTTGCTCAAAATAGTTTGCTTGCACTGCGTACTCCGCTCTTCATCAACAATAAGATCAATGGTGGTTGTAGTACTAACAGTGATGATGATGACCAGACAAAGTCAAGTGCAACATACGAACAGGAAGCTGGTCGAAAGATGGCTGAAACGTCTTTTGATGACCATGTAGGTCGCCAAATTGTGATACCATGGTGGCCGACACCAGTTCCTAGACTTCCACAGCGCTCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

213

Amino Acids

24.17

Weight (kDa)

8.39

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 22 - 139 2.5e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 442
AccII CGCG 1 cut(s) 340
AciI CCGC 3 cut(s) 36, 338, 440
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 2 cut(s) 240, 393
AfaI GTAC 5 cut(s) 256, 330, 353, 436, 478
AfeI AGCGCT 1 cut(s) 629
AfiI CCNNNNNNNGG 2 cut(s) 131, 222
AgsI TTSAA 2 cut(s) 229, 349
AjnI CCWGG 2 cut(s) 130, 285
AjuI GAANNNNNNNTTGG 2 cut(s) 385, 417
AluBI AGCT 3 cut(s) 326, 378, 533
AluI AGCT 3 cut(s) 326, 378, 533
Alw26I GTCTC 1 cut(s) 296
Aor51HI AGCGCT 1 cut(s) 629
AoxI GGCC 1 cut(s) 599
ApoI RAATTY 2 cut(s) 240, 393
AseI ATTAAT 1 cut(s) 279
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 91, 320
AvaII GGWCC 1 cut(s) 248
AxyI CCTNAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 581, 614
BanI GGYRCC 1 cut(s) 51
BauI CACGAG 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 283
BccI CCATC 1 cut(s) 538
BciT130I CCWGG 2 cut(s) 132, 287
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 4 cut(s) 48, 252, 297, 615
BfoI RGCGCY 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 44
BmcAI AGTACT 2 cut(s) 353, 478
Bme1390I CCNGG 2 cut(s) 132, 287
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 2 cut(s) 132, 287
BmsI GCATC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 283
BsaJI CCNNGG 3 cut(s) 130, 152, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 222
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 4 cut(s) 114, 189, 310, 608
Bse21I CCTNAGG 1 cut(s) 362
BseBI CCWGG 2 cut(s) 132, 287
BseDI CCNNGG 3 cut(s) 130, 152, 593
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 131, 222
BseNI ACTGG 4 cut(s) 114, 189, 310, 608
BseSI GKGCMC 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 601
BshNI GGYRCC 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 73
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 131, 222
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 1 cut(s) 459
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 3 cut(s) 36, 338, 440
BspANI GGCC 1 cut(s) 601
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 53
BspQI GCTCTTC 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 4 cut(s) 114, 189, 310, 608
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 3 cut(s) 130, 152, 593
BssMI GATC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 152, 593
Bst2BI CACGAG 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 132, 287
Bst4CI ACNGT 4 cut(s) 25, 74, 103, 485
Bst6I CTCTTC 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 45, 426
BstDEI CTNAG 2 cut(s) 362, 631
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 1 cut(s) 462
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 459
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 4 cut(s) 44, 194, 430, 439
BstNI CCWGG 2 cut(s) 132, 287
BstSCI CCNGG 2 cut(s) 130, 285
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 283
Bsu36I CCTNAGG 1 cut(s) 362
BsuRI GGCC 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 2 cut(s) 428, 490
Cac8I GCNNGC 2 cut(s) 45, 426
CfoI GCGC 1 cut(s) 630
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 5 cut(s) 255, 329, 352, 435, 477
CviAII CATG 3 cut(s) 333, 569, 594
CviJI RGCY 9 cut(s) 47, 172, 188, 326, 345, 378, 533, 548, 601
CviKI_1 RGCY 9 cut(s) 47, 172, 188, 326, 345, 378, 533, 548, 601
CviQI GTAC 5 cut(s) 255, 329, 352, 435, 477
DdeI CTNAG 2 cut(s) 362, 631
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 152, 593
Eco47I GGWCC 1 cut(s) 248
Eco47III AGCGCT 1 cut(s) 629
Eco81I CCTNAGG 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 130, 285
EcoT14I CCWWGG 2 cut(s) 152, 593
ErhI CCWWGG 2 cut(s) 152, 593
FaeI CATG 3 cut(s) 336, 572, 597
FaiI YATR 7 cut(s) 60, 222, 334, 520, 570, 595, 637
FalI AAGNNNNNCTT 2 cut(s) 136, 168
FatI CATG 3 cut(s) 332, 568, 593
FokI GGATG 1 cut(s) 147
FspBI CTAG 4 cut(s) 48, 252, 297, 615
GlaI GCGC 1 cut(s) 629
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 1 cut(s) 601
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 630
Hin1II CATG 3 cut(s) 336, 572, 597
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HinfI GANTC 1 cut(s) 10
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 91, 320
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 71
HpyCH4III ACNGT 4 cut(s) 25, 74, 103, 485
HpyCH4IV ACGT 1 cut(s) 554
HpyCH4V TGCA 4 cut(s) 90, 106, 428, 515
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 194, 430, 439
HpyF3I CTNAG 2 cut(s) 362, 631
HpySE526I ACGT 1 cut(s) 554
Hsp92II CATG 3 cut(s) 336, 572, 597
HspAI GCGC 1 cut(s) 628
Kzo9I GATC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 449
LweI GCATC 1 cut(s) 125
MaeI CTAG 4 cut(s) 48, 252, 297, 615
MaeII ACGT 1 cut(s) 554
MaeIII GTNAC 4 cut(s) 7, 25, 269, 383
MalI GATC 1 cut(s) 461
MbiI CCGCTC 1 cut(s) 442
MboI GATC 1 cut(s) 459
MboII GAAGA 3 cut(s) 110, 283, 436
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 4 cut(s) 240, 276, 393, 582
MlyI GAGTC 1 cut(s) 4
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 2 cut(s) 132, 335
MseI TTAA 4 cut(s) 114, 204, 210, 279
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 2 cut(s) 132, 287
MvaI CCWGG 2 cut(s) 132, 287
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 4 cut(s) 44, 194, 430, 439
NcoI CCATGG 1 cut(s) 593
NdeII GATC 1 cut(s) 459
NlaIII CATG 3 cut(s) 336, 572, 597
NlaIV GGNNCC 1 cut(s) 53
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 7, 25
PciSI GCTCTTC 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 505
PleI GAGTC 1 cut(s) 4
PpsI GAGTC 1 cut(s) 4
PshBI ATTAAT 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 130, 285
PspGI CCWGG 2 cut(s) 130, 285
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 1 cut(s) 248
PsyI GACNNNGTC 1 cut(s) 505
RsaI GTAC 5 cut(s) 256, 330, 353, 436, 478
RsaNI GTAC 5 cut(s) 255, 329, 352, 435, 477
RseI CAYNNNNRTG 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 449
SaqAI TTAA 4 cut(s) 114, 204, 210, 279
Sau3AI GATC 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 248
ScaI AGTACT 2 cut(s) 353, 478
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 132, 287
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 1 cut(s) 125
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 23
Sse9I AATT 4 cut(s) 240, 276, 393, 582
SsiI CCGC 3 cut(s) 36, 338, 440
SspMI CTAG 4 cut(s) 48, 252, 297, 615
StyD4I CCNGG 2 cut(s) 130, 285
StyI CCWWGG 2 cut(s) 152, 593
TaaI ACNGT 4 cut(s) 25, 74, 103, 485
TaiI ACGT 1 cut(s) 557
TaqI TCGA 3 cut(s) 66, 357, 538
TasI AATT 4 cut(s) 240, 276, 393, 582
TatI WGTACW 3 cut(s) 254, 351, 476
Tru1I TTAA 4 cut(s) 114, 204, 210, 279
Tru9I TTAA 4 cut(s) 114, 204, 210, 279
TscAI CASTG 2 cut(s) 435, 490
TseFI GTSAC 2 cut(s) 7, 25
Tsp45I GTSAC 2 cut(s) 7, 25
TspDTI ATGAA 2 cut(s) 151, 436
TspRI CASTG 2 cut(s) 435, 490
Tth111I GACNNNGTC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 248
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 2 cut(s) 240, 393
XspI CTAG 4 cut(s) 48, 252, 297, 615
ZrmI AGTACT 2 cut(s) 353, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.