Rh5DG568500

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001837
Physical Location & Seq
Reverse (-)
87522928 .. 87524655
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG568500.1

Sequence Viewer

Length: 1728 bp
ATGAAAGGTGACAGAAATCCTGCCACTAAAAGAAAATATAGTAGAATATCAGTACCAACTGATTCCTCTGATGGCTTAAGAAGATCGAAAAAGTCAGCTGCGAAAAGATCATCATCAACTAATATATATGATCTCCCGAAGGAATTATTAGTTGAAATCCTTTCTCGACTGCCTTGCGCAAAATCTGTCTGTCGATGTAAGTTTGTTTCAAAGAGTTGGTGGACTCTCATGTCAGATCCTTATTTTGTTGGCCGCTTTTTGTGGCTACAGAGAAATAACCAAACCCTAATACCTGGCACTATTATAAGTAATAAGGCCGAAGAACTTACCACAAACGACGTGCTGGCAAGATTAAGGAATTTCTTCAAACTCAAGAAGAAGAAATTACAAGTGCTAGCTACATGGAATGACTTGGTTCTCTGCTGCACAGTCAGCGGAGGCTATTGCTATTACTACGTCTGCAATCCACGGACCATGCAACTGGTTCATCTTCCTCGCATCGCTCAATACCCTGGTCTCCCTGATATATTCGACTATCAGCCTGTGGGATTCATCTGTGAGCCCTACAATAAGGAAGACAGGGTCGTCGTCCACAGTGATCAACAACAAGAACAAAAAGAAGAAAGGAAGGTAATTAAAATTAATGCCGAGTACAAGTGTAAGGTTGTCAGAATTCTTCCTCCTGATGAGATAATAAAACCTTGTTTCGAATTCAAGATTCAGGTATTCTCTAGTGAGACTGGTGAATGGAGAGAATCACTAGTGGCATGTCCACAAGGCTTTATTTATAAACAACTCGGGGATGTATCTGTCTGTGCTACCAATGGCATGCTATATTGGAATAATGCTGACTATGGTGATCTGCTCGTGCTGGAACCGTTGATCAACAACAAGAGTACTACTACTACTGCCAATGATGATTCTGGCTATCAATTTCGTGTTATTAAATATGTTCAGGAAGATGATGTTCCTTATCCTCATCAGCCATTTTCGCCTTATCACCTTGGCGGGCAGCAGGGACGTCTGCTGTTGGTGCGTACAGTGGCGAATGGATTCCCCGACATGGGCGCTAGGCTATTCATTTGGGAGTTGGATCAAGAAAGGGGTGGAGAACTCTGTCGTCGGAGGACTTACAACATACTGGACAAAGAATTTCTTGGTTTAGAGACTGGGGTGTCTTGTATAGGTTTCGACCAAAATGATTTGGATATATGTTATGTACTTGTATGTCATCACGAGGAACTAGAAGGCCTTCACAAGTACAACCTTCACACAGGGAAACGGTCCATGATAAACAAAGAATTTTATGGGTGCGCATTACAAGTTATGCTTCCGTGGTGGTCAGTTCCTACAATATCCGATCCCATGCCGGATAGGAATACCGAGATCAACGGGAGTGAGGAAGAAGGTGCCGCGCTCAGATATATAGCAAGATCAAAAGTGAAGAAAACCAAAGATGTGGATGGTGTTGTGAATGGAGAGGAGGGTGCGGGTTTTCAAGCTCCAAGGTTGAGTGGTAGAAAGCACGGCATTGATGTGGAGAAGAGTGGTGCAACAAAGAGGTTCAAGGCGATGGATGTAGTAGTCCCAGCTAATGCTCCTAAGGAAGTGCATGCTTCAAATCCCACTTCGTCCAAGGTATCACGAGAAACCACCACACGAGAAATTCTTCATCATGCTTTTGGAAACATGCCAATATTCCTTAAAAATGCTTCAAGTTCGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

575

Amino Acids

65.33

Weight (kDa)

8.69

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 43 - 81 9.6e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 305, 789
AasI GACNNNNNNGTC 3 cut(s) 229, 584, 1174
AatII GACGTC 1 cut(s) 1024
Acc16I TGCGCA 2 cut(s) 178, 1315
AccB1I GGYRCC 1 cut(s) 1409
AccII CGCG 1 cut(s) 1415
AciI CCGC 5 cut(s) 253, 435, 1008, 1413, 1490
AclWI GGATC 3 cut(s) 230, 1101, 1355
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 6 cut(s) 358, 672, 710, 1151, 1301, 1665
AcyI GRCGYC 1 cut(s) 1021
AfaI GTAC 6 cut(s) 54, 653, 898, 1039, 1221, 1262
AfiI CCNNNNNNNGG 2 cut(s) 1063, 1064
AflII CTTAAG 1 cut(s) 76
AgsI TTSAA 8 cut(s) 155, 210, 367, 715, 1499, 1567, 1620, 1716
AhlI ACTAGT 1 cut(s) 760
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 2 cut(s) 292, 511
AluBI AGCT 4 cut(s) 98, 398, 1502, 1592
AluI AGCT 4 cut(s) 98, 398, 1502, 1592
Alw26I GTCTC 3 cut(s) 521, 731, 1160
AlwI GGATC 3 cut(s) 230, 1101, 1355
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 3 cut(s) 250, 315, 1249
ApeKI GCWGC 3 cut(s) 98, 423, 1012
ApoI RAATTY 6 cut(s) 358, 672, 710, 1151, 1301, 1665
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 4 cut(s) 362, 1052, 1251, 1668
AspLEI GCGC 4 cut(s) 179, 1070, 1316, 1417
AspS9I GGNCC 2 cut(s) 471, 1284
AsuHPI GGTGA 4 cut(s) 20, 755, 869, 992
AsuII TTCGAA 1 cut(s) 708
AsuNHI GCTAGC 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 2 cut(s) 471, 1284
AxyI CCTNAGG 1 cut(s) 1602
BanI GGYRCC 1 cut(s) 1409
BanII GRGCYC 1 cut(s) 564
BauI CACGAG 4 cut(s) 866, 1235, 1644, 1659
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 3 cut(s) 85, 410, 1024
BccI CCATC 3 cut(s) 65, 1457, 1567
BceAI ACGGC 1 cut(s) 1543
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 2 cut(s) 294, 513
BclI TGATCA 2 cut(s) 598, 882
BcoDI GTCTC 3 cut(s) 521, 731, 1160
BcuI ACTAGT 1 cut(s) 760
BfaI CTAG 5 cut(s) 395, 732, 761, 1071, 1244
BfmI CTRYAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 1071
BfrI CTTAAG 1 cut(s) 76
BisI GCNGC 5 cut(s) 99, 253, 424, 1013, 1413
BlsI GCNGC 5 cut(s) 100, 254, 425, 1014, 1414
BmcAI AGTACT 1 cut(s) 898
Bme1390I CCNGG 2 cut(s) 294, 513
Bme18I GGWCC 2 cut(s) 471, 1284
BmeT110I CYCGRG 1 cut(s) 797
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 471, 1284
BmiI GGNNCC 2 cut(s) 876, 1411
BmrFI CCNGG 2 cut(s) 294, 513
BmrI ACTGGG 1 cut(s) 1179
BmsI GCATC 1 cut(s) 507
BmtI GCTAGC 1 cut(s) 398
BmuI ACTGGG 1 cut(s) 1179
BpiI GAAGAC 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 1021
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 6 cut(s) 467, 511, 1003, 1334, 1505, 1635
Bsc4I CCNNNNNNNGG 2 cut(s) 1063, 1064
Bse1I ACTGG 4 cut(s) 486, 745, 1146, 1174
Bse21I CCTNAGG 1 cut(s) 1602
Bse8I GATNNNNATC 1 cut(s) 112
BseBI CCWGG 2 cut(s) 294, 513
BseDI CCNNGG 6 cut(s) 467, 511, 1003, 1334, 1505, 1635
BseGI GGATG 3 cut(s) 808, 1468, 1582
BseJI GATNNNNATC 1 cut(s) 112
BseLI CCNNNNNNNGG 2 cut(s) 1063, 1064
BseMII CTCAG 1 cut(s) 1432
BseNI ACTGG 4 cut(s) 486, 745, 1146, 1174
BseRI GAGGAG 1 cut(s) 1496
BseXI GCAGC 3 cut(s) 85, 410, 1024
BseYI CCCAGC 1 cut(s) 1588
BsgI GTGCAG 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 1415
BshFI GGCC 3 cut(s) 252, 317, 1251
BshNI GGYRCC 1 cut(s) 1409
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 1 cut(s) 1370
BslFI GGGAC 2 cut(s) 1032, 1571
BslI CCNNNNNNNGG 2 cut(s) 1063, 1064
BsmAI GTCTC 3 cut(s) 521, 731, 1160
BsmFI GGGAC 2 cut(s) 1032, 1571
BsnI GGCC 3 cut(s) 252, 317, 1251
Bso31I GGTCTC 1 cut(s) 521
BsoBI CYCGRG 1 cut(s) 797
Bsp119I TTCGAA 1 cut(s) 708
Bsp1286I GDGCHC 1 cut(s) 564
BspACI CCGC 5 cut(s) 253, 435, 1008, 1413, 1490
BspANI GGCC 3 cut(s) 252, 317, 1251
BspCNI CTCAG 1 cut(s) 1431
BspFNI CGCG 1 cut(s) 1415
BspLI GGNNCC 2 cut(s) 876, 1411
BspOI GCTAGC 1 cut(s) 398
BspPI GGATC 3 cut(s) 230, 1101, 1355
BspT104I TTCGAA 1 cut(s) 708
BspT107I GGYRCC 1 cut(s) 1409
BspTI CTTAAG 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 521
BsrI ACTGG 4 cut(s) 486, 745, 1146, 1174
BssECI CCNNGG 6 cut(s) 467, 511, 1003, 1334, 1505, 1635
BssNI GRCGYC 1 cut(s) 1021
BssSI CACGAG 4 cut(s) 866, 1235, 1644, 1659
BssT1I CCWWGG 3 cut(s) 1003, 1505, 1635
Bst2BI CACGAG 4 cut(s) 866, 1235, 1644, 1659
Bst2UI CCWGG 2 cut(s) 294, 513
Bst4CI ACNGT 5 cut(s) 430, 596, 879, 1042, 1284
Bst6I CTCTTC 1 cut(s) 1538
BstACI GRCGYC 1 cut(s) 1021
BstAFI CTTAAG 1 cut(s) 76
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 5 cut(s) 345, 396, 830, 1010, 1614
BstDEI CTNAG 2 cut(s) 1418, 1602
BstDSI CCRYGG 2 cut(s) 467, 1334
BstF5I GGATG 3 cut(s) 808, 1468, 1582
BstFNI CGCG 1 cut(s) 1415
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 4 cut(s) 179, 1070, 1316, 1417
BstMAI GTCTC 3 cut(s) 521, 731, 1160
BstMWI GCNNNNNNNGC 3 cut(s) 432, 991, 1033
BstNI CCWGG 2 cut(s) 294, 513
BstNSI RCATGY 4 cut(s) 771, 832, 1616, 1693
BstSCI CCNGG 2 cut(s) 292, 511
BstSFI CTRYAG 1 cut(s) 266
BstUI CGCG 1 cut(s) 1415
BstV1I GCAGC 3 cut(s) 85, 410, 1024
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 235
BstXI CCANNNNNNTGG 2 cut(s) 481, 1459
BstYI RGATCY 1 cut(s) 235
Bsu36I CCTNAGG 1 cut(s) 1602
BsuRI GGCC 3 cut(s) 252, 317, 1251
BtgI CCRYGG 2 cut(s) 467, 1334
BtgZI GCGATG 2 cut(s) 484, 1586
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 808, 1468, 1582
BtsIMutI CAGTG 2 cut(s) 601, 1047
Cac8I GCNNGC 5 cut(s) 345, 396, 830, 1010, 1614
CfoI GCGC 4 cut(s) 179, 1070, 1316, 1417
Cfr13I GGNCC 2 cut(s) 471, 1284
Csp6I GTAC 6 cut(s) 53, 652, 897, 1038, 1220, 1261
CviQI GTAC 6 cut(s) 53, 652, 897, 1038, 1220, 1261
DdeI CTNAG 2 cut(s) 1418, 1602
DrdI GACNNNNNNGTC 3 cut(s) 229, 584, 1174
DseDI GACNNNNNNGTC 3 cut(s) 229, 584, 1174
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 1538
EarI CTCTTC 1 cut(s) 1538
Eco130I CCWWGG 3 cut(s) 1003, 1505, 1635
Eco147I AGGCCT 1 cut(s) 1251
Eco24I GRGCYC 1 cut(s) 564
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 471, 1284
Eco81I CCTNAGG 1 cut(s) 1602
Eco88I CYCGRG 1 cut(s) 797
EcoRI GAATTC 2 cut(s) 672, 710
EcoRII CCWGG 2 cut(s) 292, 511
EcoT14I CCWWGG 3 cut(s) 1003, 1505, 1635
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 3 cut(s) 1003, 1505, 1635
FalI AAGNNNNNCTT 4 cut(s) 1140, 1172, 1314, 1346
FaqI GGGAC 2 cut(s) 1032, 1571
FauI CCCGC 2 cut(s) 1001, 1483
FbaI TGATCA 2 cut(s) 598, 882
Fnu4HI GCNGC 5 cut(s) 99, 253, 424, 1013, 1413
FokI GGATG 3 cut(s) 815, 1475, 1589
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 5 cut(s) 99, 253, 424, 1013, 1413
FspAI RTGCGCAY 1 cut(s) 1315
FspBI CTAG 5 cut(s) 395, 732, 761, 1071, 1244
FspI TGCGCA 2 cut(s) 178, 1315
GlaI GCGC 4 cut(s) 178, 1069, 1315, 1416
GluI GCNGC 5 cut(s) 99, 253, 424, 1013, 1413
GsaI CCCAGC 1 cut(s) 1592
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 3 cut(s) 252, 317, 1251
HapII CCGG 1 cut(s) 1370
HhaI GCGC 4 cut(s) 179, 1070, 1316, 1417
Hin1I GRCGYC 1 cut(s) 1021
Hin6I GCGC 4 cut(s) 177, 1068, 1314, 1415
HinP1I GCGC 4 cut(s) 177, 1068, 1314, 1415
HinfI GANTC 7 cut(s) 62, 223, 549, 718, 755, 920, 1053
HpaII CCGG 1 cut(s) 1370
HphI GGTGA 4 cut(s) 20, 755, 869, 992
Hpy166II GTNNAC 3 cut(s) 222, 592, 773
Hpy188I TCNGA 6 cut(s) 70, 235, 671, 1125, 1360, 1421
Hpy188III TCNNGA 9 cut(s) 136, 165, 373, 683, 715, 956, 1097, 1235, 1644
Hpy8I GTNNAC 3 cut(s) 222, 592, 773
Hpy99I CGWCG 3 cut(s) 341, 590, 1125
HpyAV CCTTC 6 cut(s) 133, 622, 1241, 1262, 1277, 1400
HpyCH4III ACNGT 5 cut(s) 430, 596, 879, 1042, 1284
HpyCH4IV ACGT 3 cut(s) 339, 456, 1021
HpyCH4V TGCA 5 cut(s) 426, 462, 478, 1553, 1612
HpyF10VI GCNNNNNNNGC 3 cut(s) 432, 991, 1033
HpyF3I CTNAG 2 cut(s) 1418, 1602
HpySE526I ACGT 3 cut(s) 339, 456, 1021
Hsp92I GRCGYC 1 cut(s) 1021
HspAI GCGC 4 cut(s) 177, 1068, 1314, 1415
Ksp22I TGATCA 2 cut(s) 598, 882
LmnI GCTCC 2 cut(s) 1507, 1603
Lsp1109I GCAGC 3 cut(s) 85, 410, 1024
LweI GCATC 1 cut(s) 507
MaeI CTAG 5 cut(s) 395, 732, 761, 1071, 1244
MaeII ACGT 3 cut(s) 339, 456, 1021
MaeIII GTNAC 1 cut(s) 8
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 564
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 2 cut(s) 1071, 1103
MroXI GAANNNNTTC 4 cut(s) 362, 1052, 1251, 1668
MseI TTAA 7 cut(s) 77, 353, 636, 642, 945, 1704, 1726
MslI CAYNNNNRTG 1 cut(s) 1535
MspA1I CMGCKG 2 cut(s) 98, 435
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 1 cut(s) 1370
MspR9I CCNGG 2 cut(s) 294, 513
MvaI CCWGG 2 cut(s) 294, 513
MvnI CGCG 1 cut(s) 1415
MwoI GCNNNNNNNGC 3 cut(s) 432, 991, 1033
NheI GCTAGC 1 cut(s) 394
NlaIV GGNNCC 2 cut(s) 876, 1411
NmeAIII GCCGAG 1 cut(s) 673
NmuCI GTSAC 1 cut(s) 8
NsbI TGCGCA 2 cut(s) 178, 1315
NspI RCATGY 4 cut(s) 771, 832, 1616, 1693
NspV TTCGAA 1 cut(s) 708
PaeI GCATGC 2 cut(s) 832, 1616
PceI AGGCCT 1 cut(s) 1251
PdmI GAANNNNTTC 4 cut(s) 362, 1052, 1251, 1668
PfeI GAWTC 6 cut(s) 62, 549, 718, 755, 920, 1053
PflFI GACNNNGTC 1 cut(s) 581
PkrI GCNGC 5 cut(s) 100, 254, 425, 1014, 1414
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PshBI ATTAAT 1 cut(s) 642
PsiI TTATAA 2 cut(s) 305, 789
Psp6I CCWGG 2 cut(s) 292, 511
PspFI CCCAGC 1 cut(s) 1588
PspGI CCWGG 2 cut(s) 292, 511
PspN4I GGNNCC 2 cut(s) 876, 1411
PspPI GGNCC 2 cut(s) 471, 1284
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 6 cut(s) 54, 653, 898, 1039, 1221, 1262
RsaNI GTAC 6 cut(s) 53, 652, 897, 1038, 1220, 1261
RseI CAYNNNNRTG 1 cut(s) 1535
SaqAI TTAA 7 cut(s) 77, 353, 636, 642, 945, 1704, 1726
SatI GCNGC 5 cut(s) 99, 253, 424, 1013, 1413
Sau96I GGNCC 2 cut(s) 471, 1284
ScaI AGTACT 1 cut(s) 898
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 294, 513
SduI GDGCHC 1 cut(s) 564
SfaNI GCATC 1 cut(s) 507
SfcI CTRYAG 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 2 cut(s) 471, 1284
SmiMI CAYNNNNRTG 1 cut(s) 1535
SmlI CTYRAG 2 cut(s) 76, 371
SmoI CTYRAG 2 cut(s) 76, 371
SpeI ACTAGT 1 cut(s) 760
SphI GCATGC 2 cut(s) 832, 1616
SseBI AGGCCT 1 cut(s) 1251
SsiI CCGC 5 cut(s) 253, 435, 1008, 1413, 1490
SspI AATATT 1 cut(s) 1698
SspMI CTAG 5 cut(s) 395, 732, 761, 1071, 1244
StuI AGGCCT 1 cut(s) 1251
StyD4I CCNGG 2 cut(s) 292, 511
StyI CCWWGG 3 cut(s) 1003, 1505, 1635
TaaI ACNGT 5 cut(s) 430, 596, 879, 1042, 1284
TaiI ACGT 3 cut(s) 342, 459, 1024
TaqI TCGA 6 cut(s) 86, 166, 193, 531, 708, 1191
TatI WGTACW 4 cut(s) 651, 896, 1219, 1260
TauI GCSGC 2 cut(s) 255, 1415
TfiI GAWTC 6 cut(s) 62, 549, 718, 755, 920, 1053
Tru1I TTAA 7 cut(s) 77, 353, 636, 642, 945, 1704, 1726
Tru9I TTAA 7 cut(s) 77, 353, 636, 642, 945, 1704, 1726
TscAI CASTG 2 cut(s) 601, 1047
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 98, 423, 1012
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 5 cut(s) 17, 476, 541, 1069, 1661
TspGWI ACGGA 2 cut(s) 484, 1323
TspRI CASTG 2 cut(s) 601, 1047
Tth111I GACNNNGTC 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 471, 1284
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 6 cut(s) 358, 672, 710, 1151, 1301, 1665
XceI RCATGY 4 cut(s) 771, 832, 1616, 1693
XmnI GAANNNNTTC 4 cut(s) 362, 1052, 1251, 1668
XspI CTAG 5 cut(s) 395, 732, 761, 1071, 1244
ZraI GACGTC 1 cut(s) 1022
ZrmI AGTACT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.