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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 249 |
| AbsI | CCTCGAGG | 1 cut(s) | 1727 |
| AccB1I | GGYRCC | 1 cut(s) | 419 |
| AccBSI | CCGCTC | 1 cut(s) | 177 |
| AccI | GTMKAC | 2 cut(s) | 153, 585 |
| AccII | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| AccIII | TCCGGA | 2 cut(s) | 1279, 1762 |
| AclWI | GGATC | 3 cut(s) | 90, 693, 1631 |
| AcoI | YGGCCR | 3 cut(s) | 3, 549, 1191 |
| AcsI | RAATTY | 2 cut(s) | 830, 970 |
| AcuI | CTGAAG | 1 cut(s) | 632 |
| AcyI | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| AfaI | GTAC | 2 cut(s) | 1656, 1723 |
| AfeI | AGCGCT | 1 cut(s) | 1078 |
| AgsI | TTSAA | 5 cut(s) | 622, 718, 1150, 1358, 1543 |
| AjnI | CCWGG | 3 cut(s) | 138, 312, 551 |
| Alw21I | GWGCWC | 2 cut(s) | 60, 1825 |
| Alw26I | GTCTC | 6 cut(s) | 358, 547, 744, 778, 870, 1163 |
| AlwI | GGATC | 3 cut(s) | 90, 693, 1631 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1268 |
| Ama87I | CYCGRG | 1 cut(s) | 1727 |
| Aor13HI | TCCGGA | 2 cut(s) | 1279, 1762 |
| Aor51HI | AGCGCT | 1 cut(s) | 1078 |
| AoxI | GGCC | 9 cut(s) | 3, 99, 108, 141, 502, 525, 549, 854, 1191 |
| ApeKI | GCWGC | 7 cut(s) | 607, 1039, 1225, 1259, 1319, 1502, 1795 |
| ApoI | RAATTY | 2 cut(s) | 830, 970 |
| AspS9I | GGNCC | 8 cut(s) | 88, 100, 142, 477, 502, 514, 1451, 1611 |
| AsuC2I | CCSGG | 5 cut(s) | 26, 512, 1373, 1441, 1818 |
| AsuHPI | GGTGA | 4 cut(s) | 241, 319, 793, 1096 |
| AsuII | TTCGAA | 1 cut(s) | 1001 |
| AvaI | CYCGRG | 1 cut(s) | 1727 |
| AvaII | GGWCC | 5 cut(s) | 88, 477, 514, 1451, 1611 |
| BalI | TGGCCA | 2 cut(s) | 551, 1193 |
| BanI | GGYRCC | 1 cut(s) | 419 |
| BanII | GRGCYC | 2 cut(s) | 60, 1825 |
| BauI | CACGAG | 1 cut(s) | 104 |
| BbsI | GAAGAC | 2 cut(s) | 310, 397 |
| Bbv12I | GWGCWC | 2 cut(s) | 60, 1825 |
| BbvCI | CCTCAGC | 1 cut(s) | 1080 |
| BbvI | GCAGC | 7 cut(s) | 594, 1026, 1237, 1246, 1306, 1489, 1807 |
| BccI | CCATC | 7 cut(s) | 77, 332, 383, 500, 1013, 1243, 1574 |
| BceAI | ACGGC | 4 cut(s) | 167, 433, 841, 1733 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1389, 1423, 1681, 1715 |
| BciT130I | CCWGG | 3 cut(s) | 140, 314, 553 |
| BciVI | GTATCC | 1 cut(s) | 874 |
| BclI | TGATCA | 1 cut(s) | 1336 |
| BcnI | CCSGG | 5 cut(s) | 26, 512, 1373, 1441, 1818 |
| BcoDI | GTCTC | 6 cut(s) | 358, 547, 744, 778, 870, 1163 |
| BfaI | CTAG | 4 cut(s) | 278, 837, 852, 921 |
| BfmI | CTRYAG | 2 cut(s) | 905, 1260 |
| BfoI | RGCGCY | 3 cut(s) | 223, 423, 1080 |
| BfuI | GTATCC | 1 cut(s) | 874 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 107, 300 |
| BmcAI | AGTACT | 1 cut(s) | 1656 |
| Bme1390I | CCNGG | 8 cut(s) | 26, 140, 314, 512, 553, 1373, 1441, 1818 |
| Bme18I | GGWCC | 5 cut(s) | 88, 477, 514, 1451, 1611 |
| BmeT110I | CYCGRG | 1 cut(s) | 1727 |
| BmgT120I | GGNCC | 8 cut(s) | 88, 100, 142, 477, 502, 514, 1451, 1611 |
| BmiI | GGNNCC | 5 cut(s) | 89, 395, 421, 515, 1587 |
| BmrFI | CCNGG | 8 cut(s) | 26, 140, 314, 512, 553, 1373, 1441, 1818 |
| BmsI | GCATC | 3 cut(s) | 198, 243, 1807 |
| BpiI | GAAGAC | 2 cut(s) | 310, 397 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 44, 76 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1080 |
| Bpu14I | TTCGAA | 1 cut(s) | 1001 |
| BpuEI | CTTGAG | 2 cut(s) | 1076, 1082 |
| BpuMI | CCSGG | 5 cut(s) | 26, 512, 1373, 1441, 1818 |
| BsaAI | YACGTR | 1 cut(s) | 1681 |
| BsaHI | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| BsaJI | CCNNGG | 5 cut(s) | 24, 552, 1113, 1497, 1749 |
| BsaWI | WCCGGW | 3 cut(s) | 564, 1279, 1762 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 146, 176, 617, 647 |
| Bse118I | RCCGGY | 1 cut(s) | 1387 |
| Bse1I | ACTGG | 1 cut(s) | 792 |
| Bse3DI | GCAATG | 1 cut(s) | 1506 |
| BseAI | TCCGGA | 2 cut(s) | 1279, 1762 |
| BseBI | CCWGG | 3 cut(s) | 140, 314, 553 |
| BseDI | CCNNGG | 5 cut(s) | 24, 552, 1113, 1497, 1749 |
| BseGI | GGATG | 5 cut(s) | 69, 492, 898, 1492, 1825 |
| BseMI | GCAATG | 1 cut(s) | 1506 |
| BseMII | CTCAG | 4 cut(s) | 478, 1071, 1769, 1816 |
| BseNI | ACTGG | 1 cut(s) | 792 |
| BsePI | GCGCGC | 1 cut(s) | 1739 |
| BseRI | GAGGAG | 4 cut(s) | 65, 68, 353, 386 |
| BseXI | GCAGC | 7 cut(s) | 594, 1026, 1237, 1246, 1306, 1489, 1807 |
| BseYI | CCCAGC | 1 cut(s) | 468 |
| Bsh1236I | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| Bsh1285I | CGRYCG | 6 cut(s) | 157, 265, 444, 565, 819, 1295 |
| BshFI | GGCC | 9 cut(s) | 5, 101, 110, 143, 504, 527, 551, 856, 1193 |
| BshNI | GGYRCC | 1 cut(s) | 419 |
| BsiEI | CGRYCG | 6 cut(s) | 157, 265, 444, 565, 819, 1295 |
| BsiHKAI | GWGCWC | 2 cut(s) | 60, 1825 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1727 |
| BslFI | GGGAC | 2 cut(s) | 527, 569 |
| BsmAI | GTCTC | 6 cut(s) | 358, 547, 744, 778, 870, 1163 |
| BsmBI | CGTCTC | 2 cut(s) | 547, 1163 |
| BsmFI | GGGAC | 2 cut(s) | 527, 569 |
| BsmI | GAATGC | 4 cut(s) | 134, 667, 924, 1555 |
| BsnI | GGCC | 9 cut(s) | 5, 101, 110, 143, 504, 527, 551, 856, 1193 |
| BsoBI | CYCGRG | 1 cut(s) | 1727 |
| Bsp119I | TTCGAA | 1 cut(s) | 1001 |
| Bsp1286I | GDGCHC | 2 cut(s) | 60, 1825 |
| Bsp13I | TCCGGA | 2 cut(s) | 1279, 1762 |
| Bsp143I | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| BspANI | GGCC | 9 cut(s) | 5, 101, 110, 143, 504, 527, 551, 856, 1193 |
| BspCNI | CTCAG | 4 cut(s) | 477, 1072, 1768, 1815 |
| BspEI | TCCGGA | 2 cut(s) | 1279, 1762 |
| BspFNI | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| BspLI | GGNNCC | 5 cut(s) | 89, 395, 421, 515, 1587 |
| BspMAI | CTGCAG | 2 cut(s) | 909, 1264 |
| BspPI | GGATC | 3 cut(s) | 90, 693, 1631 |
| BspT104I | TTCGAA | 1 cut(s) | 1001 |
| BspT107I | GGYRCC | 1 cut(s) | 419 |
| BsrBI | CCGCTC | 1 cut(s) | 177 |
| BsrDI | GCAATG | 1 cut(s) | 1506 |
| BsrFI | RCCGGY | 1 cut(s) | 1387 |
| BsrI | ACTGG | 1 cut(s) | 792 |
| BssAI | RCCGGY | 1 cut(s) | 1387 |
| BssECI | CCNNGG | 5 cut(s) | 24, 552, 1113, 1497, 1749 |
| BssHII | GCGCGC | 1 cut(s) | 1739 |
| BssMI | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| BssNI | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| BssSI | CACGAG | 1 cut(s) | 104 |
| Bst2BI | CACGAG | 1 cut(s) | 104 |
| Bst2UI | CCWGG | 3 cut(s) | 140, 314, 553 |
| Bst4CI | ACNGT | 3 cut(s) | 886, 1246, 1662 |
| Bst6I | CTCTTC | 1 cut(s) | 1356 |
| BstACI | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| BstBAI | YACGTR | 1 cut(s) | 1681 |
| BstBI | TTCGAA | 1 cut(s) | 1001 |
| BstC8I | GCNNGC | 5 cut(s) | 448, 506, 549, 1413, 1741 |
| BstDEI | CTNAG | 7 cut(s) | 464, 1080, 1286, 1560, 1651, 1755, 1802 |
| BstDSI | CCRYGG | 1 cut(s) | 1749 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 50, 311 |
| BstF5I | GGATG | 5 cut(s) | 69, 492, 898, 1492, 1825 |
| BstFNI | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| BstH2I | RGCGCY | 3 cut(s) | 223, 423, 1080 |
| BstKTI | GATC | 9 cut(s) | 85, 444, 579, 688, 819, 1295, 1339, 1621, 1626 |
| BstMAI | GTCTC | 6 cut(s) | 358, 547, 744, 778, 870, 1163 |
| BstMBI | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| BstMCI | CGRYCG | 6 cut(s) | 157, 265, 444, 565, 819, 1295 |
| BstNI | CCWGG | 3 cut(s) | 140, 314, 553 |
| BstNSI | RCATGY | 3 cut(s) | 172, 1645, 1849 |
| BstSCI | CCNGG | 8 cut(s) | 24, 138, 312, 510, 551, 1371, 1439, 1816 |
| BstSFI | CTRYAG | 2 cut(s) | 905, 1260 |
| BstUI | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| BstV1I | GCAGC | 7 cut(s) | 594, 1026, 1237, 1246, 1306, 1489, 1807 |
| BstV2I | GAAGAC | 2 cut(s) | 310, 397 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 499, 1513 |
| BsuI | GTATCC | 1 cut(s) | 874 |
| BsuRI | GGCC | 9 cut(s) | 5, 101, 110, 143, 504, 527, 551, 856, 1193 |
| BtgI | CCRYGG | 1 cut(s) | 1749 |
| BtgZI | GCGATG | 2 cut(s) | 220, 469 |
| BtsCI | GGATG | 5 cut(s) | 69, 492, 898, 1492, 1825 |
| BtsI | GCAGTG | 2 cut(s) | 1269, 1645 |
| BtsIMutI | CAGTG | 3 cut(s) | 1269, 1614, 1645 |
| Cac8I | GCNNGC | 5 cut(s) | 448, 506, 549, 1413, 1741 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1268 |
| Cfr10I | RCCGGY | 1 cut(s) | 1387 |
| Cfr13I | GGNCC | 8 cut(s) | 88, 100, 142, 477, 502, 514, 1451, 1611 |
| CseI | GACGC | 2 cut(s) | 292, 610 |
| Csp6I | GTAC | 2 cut(s) | 1655, 1722 |
| CviAII | CATG | 5 cut(s) | 169, 385, 1642, 1792, 1846 |
| CviQI | GTAC | 2 cut(s) | 1655, 1722 |
| DdeI | CTNAG | 7 cut(s) | 464, 1080, 1286, 1560, 1651, 1755, 1802 |
| DinI | GGCGCC | 1 cut(s) | 421 |
| DpnI | GATC | 9 cut(s) | 84, 443, 578, 687, 818, 1294, 1338, 1620, 1625 |
| DpnII | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| DraI | TTTAAA | 1 cut(s) | 1770 |
| EaeI | YGGCCR | 3 cut(s) | 3, 549, 1191 |
| Eam1104I | CTCTTC | 1 cut(s) | 1356 |
| EarI | CTCTTC | 1 cut(s) | 1356 |
| EciI | GGCGGA | 6 cut(s) | 169, 218, 299, 609, 809, 818 |
| Ecl136II | GAGCTC | 2 cut(s) | 58, 1823 |
| Eco24I | GRGCYC | 2 cut(s) | 60, 1825 |
| Eco47I | GGWCC | 5 cut(s) | 88, 477, 514, 1451, 1611 |
| Eco47III | AGCGCT | 1 cut(s) | 1078 |
| Eco53kI | GAGCTC | 2 cut(s) | 58, 1823 |
| Eco57I | CTGAAG | 1 cut(s) | 632 |
| Eco88I | CYCGRG | 1 cut(s) | 1727 |
| EcoICRI | GAGCTC | 2 cut(s) | 58, 1823 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 50, 311 |
| EcoO109I | RGGNCCY | 1 cut(s) | 88 |
| EcoRI | GAATTC | 1 cut(s) | 970 |
| EcoRII | CCWGG | 3 cut(s) | 138, 312, 551 |
| EcoT38I | GRGCYC | 2 cut(s) | 60, 1825 |
| EgeI | GGCGCC | 1 cut(s) | 421 |
| EheI | GGCGCC | 1 cut(s) | 421 |
| Esp3I | CGTCTC | 2 cut(s) | 547, 1163 |
| FaeI | CATG | 5 cut(s) | 172, 388, 1645, 1795, 1849 |
| FaqI | GGGAC | 2 cut(s) | 527, 569 |
| FatI | CATG | 5 cut(s) | 168, 384, 1641, 1791, 1845 |
| FbaI | TGATCA | 1 cut(s) | 1336 |
| FblI | GTMKAC | 2 cut(s) | 153, 585 |
| FokI | GGATG | 5 cut(s) | 56, 479, 905, 1499, 1812 |
| FriOI | GRGCYC | 2 cut(s) | 60, 1825 |
| FspBI | CTAG | 4 cut(s) | 278, 837, 852, 921 |
| GsaI | CCCAGC | 1 cut(s) | 472 |
| HaeII | RGCGCY | 3 cut(s) | 223, 423, 1080 |
| HaeIII | GGCC | 9 cut(s) | 5, 101, 110, 143, 504, 527, 551, 856, 1193 |
| HgaI | GACGC | 2 cut(s) | 292, 610 |
| Hin1I | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| Hin1II | CATG | 5 cut(s) | 172, 388, 1645, 1795, 1849 |
| HincII | GTYRAC | 2 cut(s) | 154, 586 |
| HindII | GTYRAC | 2 cut(s) | 154, 586 |
| HindIII | AAGCTT | 1 cut(s) | 614 |
| HphI | GGTGA | 4 cut(s) | 241, 319, 793, 1096 |
| Hpy166II | GTNNAC | 3 cut(s) | 154, 586, 1614 |
| Hpy188I | TCNGA | 8 cut(s) | 136, 244, 415, 729, 1018, 1158, 1623, 1758 |
| Hpy188III | TCNNGA | 6 cut(s) | 689, 1204, 1280, 1358, 1700, 1763 |
| Hpy8I | GTNNAC | 3 cut(s) | 154, 586, 1614 |
| Hpy99I | CGWCG | 4 cut(s) | 419, 587, 590, 637 |
| HpyAV | CCTTC | 5 cut(s) | 584, 616, 1051, 1201, 1599 |
| HpyCH4III | ACNGT | 3 cut(s) | 886, 1246, 1662 |
| HpyCH4IV | ACGT | 3 cut(s) | 246, 1673, 1680 |
| HpyF3I | CTNAG | 7 cut(s) | 464, 1080, 1286, 1560, 1651, 1755, 1802 |
| HpySE526I | ACGT | 3 cut(s) | 246, 1673, 1680 |
| Hsp92I | GRCGYC | 4 cut(s) | 246, 303, 420, 602 |
| Hsp92II | CATG | 5 cut(s) | 172, 388, 1645, 1795, 1849 |
| KasI | GGCGCC | 1 cut(s) | 419 |
| Kpn2I | TCCGGA | 2 cut(s) | 1279, 1762 |
| Ksp22I | TGATCA | 1 cut(s) | 1336 |
| Kzo9I | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| Lsp1109I | GCAGC | 7 cut(s) | 594, 1026, 1237, 1246, 1306, 1489, 1807 |
| LweI | GCATC | 3 cut(s) | 198, 243, 1807 |
| MaeI | CTAG | 4 cut(s) | 278, 837, 852, 921 |
| MaeII | ACGT | 3 cut(s) | 246, 1673, 1680 |
| MaeIII | GTNAC | 7 cut(s) | 247, 598, 847, 1162, 1367, 1383, 1664 |
| MalI | GATC | 9 cut(s) | 84, 443, 578, 687, 818, 1294, 1338, 1620, 1625 |
| MbiI | CCGCTC | 1 cut(s) | 177 |
| MboI | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| MboII | GAAGA | 4 cut(s) | 310, 397, 586, 1373 |
| MfeI | CAATTG | 1 cut(s) | 1401 |
| MhlI | GDGCHC | 2 cut(s) | 60, 1825 |
| MlsI | TGGCCA | 2 cut(s) | 551, 1193 |
| MluCI | AATT | 5 cut(s) | 830, 970, 1231, 1309, 1401 |
| MluNI | TGGCCA | 2 cut(s) | 551, 1193 |
| Mly113I | GGCGCC | 1 cut(s) | 420 |
| MlyI | GAGTC | 4 cut(s) | 122, 419, 528, 746 |
| MmeI | TCCRAC | 4 cut(s) | 159, 381, 438, 1136 |
| Mox20I | TGGCCA | 2 cut(s) | 551, 1193 |
| MroI | TCCGGA | 2 cut(s) | 1279, 1762 |
| MscI | TGGCCA | 2 cut(s) | 551, 1193 |
| MseI | TTAA | 3 cut(s) | 1230, 1302, 1769 |
| MslI | CAYNNNNRTG | 1 cut(s) | 497 |
| Msp20I | TGGCCA | 2 cut(s) | 551, 1193 |
| MspA1I | CMGCKG | 3 cut(s) | 300, 468, 607 |
| MspR9I | CCNGG | 8 cut(s) | 26, 140, 314, 512, 553, 1373, 1441, 1818 |
| MunI | CAATTG | 1 cut(s) | 1401 |
| Mva1269I | GAATGC | 4 cut(s) | 134, 667, 924, 1555 |
| MvaI | CCWGG | 3 cut(s) | 140, 314, 553 |
| MvnI | CGCG | 6 cut(s) | 80, 267, 530, 821, 1465, 1741 |
| NarI | GGCGCC | 1 cut(s) | 420 |
| NciI | CCSGG | 5 cut(s) | 26, 512, 1373, 1441, 1818 |
| NdeII | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| NlaIII | CATG | 5 cut(s) | 172, 388, 1645, 1795, 1849 |
| NlaIV | GGNNCC | 5 cut(s) | 89, 395, 421, 515, 1587 |
| NmeAIII | GCCGAG | 2 cut(s) | 136, 1138 |
| NmuCI | GTSAC | 3 cut(s) | 247, 598, 847 |
| NspI | RCATGY | 3 cut(s) | 172, 1645, 1849 |
| NspV | TTCGAA | 1 cut(s) | 1001 |
| PaeR7I | CTCGAG | 1 cut(s) | 1727 |
| PauI | GCGCGC | 1 cut(s) | 1739 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 222, 243, 450, 998 |
| PctI | GAATGC | 4 cut(s) | 134, 667, 924, 1555 |
| PfeI | GAWTC | 6 cut(s) | 368, 460, 724, 1003, 1271, 1759 |
| PflFI | GACNNNGTC | 1 cut(s) | 518 |
| PfoI | TCCNGGA | 1 cut(s) | 510 |
| Ple19I | CGATCG | 3 cut(s) | 444, 819, 1295 |
| PleI | GAGTC | 4 cut(s) | 121, 418, 527, 746 |
| PluTI | GGCGCC | 1 cut(s) | 423 |
| PpsI | GAGTC | 4 cut(s) | 121, 418, 527, 746 |
| Ppu21I | YACGTR | 1 cut(s) | 1681 |
| PpuMI | RGGWCCY | 1 cut(s) | 88 |
| Psp124BI | GAGCTC | 2 cut(s) | 60, 1825 |
| Psp5II | RGGWCCY | 1 cut(s) | 88 |
| Psp6I | CCWGG | 3 cut(s) | 138, 312, 551 |
| PspFI | CCCAGC | 1 cut(s) | 468 |
| PspGI | CCWGG | 3 cut(s) | 138, 312, 551 |
| PspN4I | GGNNCC | 5 cut(s) | 89, 395, 421, 515, 1587 |
| PspPI | GGNCC | 8 cut(s) | 88, 100, 142, 477, 502, 514, 1451, 1611 |
| PspPPI | RGGWCCY | 1 cut(s) | 88 |
| PspXI | VCTCGAGB | 1 cut(s) | 1727 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 973, 1005 |
| PstI | CTGCAG | 2 cut(s) | 909, 1264 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1268 |
| PsyI | GACNNNGTC | 1 cut(s) | 518 |
| PteI | GCGCGC | 1 cut(s) | 1739 |
| PvuI | CGATCG | 3 cut(s) | 444, 819, 1295 |
| PvuII | CAGCTG | 1 cut(s) | 468 |
| RsaI | GTAC | 2 cut(s) | 1656, 1723 |
| RsaNI | GTAC | 2 cut(s) | 1655, 1722 |
| RseI | CAYNNNNRTG | 1 cut(s) | 497 |
| SacI | GAGCTC | 2 cut(s) | 60, 1825 |
| SalI | GTCGAC | 2 cut(s) | 152, 584 |
| SaqAI | TTAA | 3 cut(s) | 1230, 1302, 1769 |
| Sau3AI | GATC | 9 cut(s) | 82, 441, 576, 685, 816, 1292, 1336, 1618, 1623 |
| Sau96I | GGNCC | 8 cut(s) | 88, 100, 142, 477, 502, 514, 1451, 1611 |
| ScaI | AGTACT | 1 cut(s) | 1656 |
| SchI | GAGTC | 4 cut(s) | 122, 419, 528, 746 |
| ScrFI | CCNGG | 8 cut(s) | 26, 140, 314, 512, 553, 1373, 1441, 1818 |
| SduI | GDGCHC | 2 cut(s) | 60, 1825 |
| SfaNI | GCATC | 3 cut(s) | 198, 243, 1807 |
| SfcI | CTRYAG | 2 cut(s) | 905, 1260 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 107 |
| SfoI | GGCGCC | 1 cut(s) | 421 |
| Sfr274I | CTCGAG | 1 cut(s) | 1727 |
| SfuI | TTCGAA | 1 cut(s) | 1001 |
| SgrDI | CGTCGACG | 1 cut(s) | 584 |
| SinI | GGWCC | 5 cut(s) | 88, 477, 514, 1451, 1611 |
| SlaI | CTCGAG | 1 cut(s) | 1727 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 497 |
| SmlI | CTYRAG | 3 cut(s) | 1091, 1097, 1727 |
| SmoI | CTYRAG | 3 cut(s) | 1091, 1097, 1727 |
| Sse9I | AATT | 5 cut(s) | 830, 970, 1231, 1309, 1401 |
| SspDI | GGCGCC | 1 cut(s) | 419 |
| SspMI | CTAG | 4 cut(s) | 278, 837, 852, 921 |
| SstI | GAGCTC | 2 cut(s) | 60, 1825 |
| StyD4I | CCNGG | 8 cut(s) | 24, 138, 312, 510, 551, 1371, 1439, 1816 |
| TaaI | ACNGT | 3 cut(s) | 886, 1246, 1662 |
| TaiI | ACGT | 3 cut(s) | 249, 1676, 1683 |
| TaqII | GACCGA | 1 cut(s) | 531 |
| TasI | AATT | 5 cut(s) | 830, 970, 1231, 1309, 1401 |
| TatI | WGTACW | 1 cut(s) | 1654 |
| TfiI | GAWTC | 6 cut(s) | 368, 460, 724, 1003, 1271, 1759 |
| Tru1I | TTAA | 3 cut(s) | 1230, 1302, 1769 |
| Tru9I | TTAA | 3 cut(s) | 1230, 1302, 1769 |
| TscAI | CASTG | 3 cut(s) | 1269, 1621, 1652 |
| TseFI | GTSAC | 3 cut(s) | 247, 598, 847 |
| TseI | GCWGC | 7 cut(s) | 607, 1039, 1225, 1259, 1319, 1502, 1795 |
| Tsp45I | GTSAC | 3 cut(s) | 247, 598, 847 |
| TspDTI | ATGAA | 4 cut(s) | 401, 662, 917, 1644 |
| TspGWI | ACGGA | 2 cut(s) | 391, 698 |
| TspRI | CASTG | 3 cut(s) | 1269, 1621, 1652 |
| Tth111I | GACNNNGTC | 1 cut(s) | 518 |
| VpaK11BI | GGWCC | 5 cut(s) | 88, 477, 514, 1451, 1611 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 50, 311 |
| XapI | RAATTY | 2 cut(s) | 830, 970 |
| XceI | RCATGY | 3 cut(s) | 172, 1645, 1849 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 496 |
| XhoI | CTCGAG | 1 cut(s) | 1727 |
| XmiI | GTMKAC | 2 cut(s) | 153, 585 |
| XspI | CTAG | 4 cut(s) | 278, 837, 852, 921 |
| ZraI | GACGTC | 1 cut(s) | 247 |
| ZrmI | AGTACT | 1 cut(s) | 1656 |