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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 787 |
| Acc16I | TGCGCA | 1 cut(s) | 795 |
| Acc36I | ACCTGC | 2 cut(s) | 277, 787 |
| AccI | GTMKAC | 1 cut(s) | 126 |
| AccII | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| AclWI | GGATC | 3 cut(s) | 63, 144, 1353 |
| AcoI | YGGCCR | 4 cut(s) | 213, 222, 309, 1161 |
| AcsI | RAATTY | 1 cut(s) | 943 |
| AcuI | CTGAAG | 1 cut(s) | 614 |
| AcyI | GRCGYC | 2 cut(s) | 276, 575 |
| AfaI | GTAC | 2 cut(s) | 952, 1693 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 25, 52, 286, 496, 667, 884 |
| AgsI | TTSAA | 6 cut(s) | 691, 1120, 1328, 1513, 1549, 1579 |
| AjiI | CACGTC | 1 cut(s) | 220 |
| AjnI | CCWGG | 5 cut(s) | 111, 231, 249, 660, 1400 |
| Alw21I | GWGCWC | 2 cut(s) | 66, 1710 |
| Alw26I | GTCTC | 3 cut(s) | 520, 843, 1133 |
| AlwI | GGATC | 3 cut(s) | 63, 144, 1353 |
| Ama87I | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| AoxI | GGCC | 8 cut(s) | 72, 81, 114, 213, 222, 309, 827, 1161 |
| ApeKI | GCWGC | 7 cut(s) | 580, 626, 880, 1195, 1229, 1289, 1472 |
| ApoI | RAATTY | 1 cut(s) | 943 |
| AseI | ATTAAT | 1 cut(s) | 915 |
| AspLEI | GCGC | 7 cut(s) | 107, 195, 242, 244, 395, 796, 1435 |
| AsuC2I | CCSGG | 4 cut(s) | 485, 885, 1343, 1411 |
| AsuHPI | GGTGA | 3 cut(s) | 238, 292, 766 |
| AsuII | TTCGAA | 1 cut(s) | 974 |
| AvaI | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| AvaII | GGWCC | 8 cut(s) | 235, 253, 450, 487, 658, 963, 1421, 1593 |
| AxyI | CCTNAGG | 1 cut(s) | 1050 |
| BalI | TGGCCA | 2 cut(s) | 224, 1163 |
| BanII | GRGCYC | 2 cut(s) | 1049, 1710 |
| BauI | CACGAG | 1 cut(s) | 77 |
| BbsI | GAAGAC | 2 cut(s) | 283, 597 |
| Bbv12I | GWGCWC | 2 cut(s) | 66, 1710 |
| BbvCI | CCTCAGC | 1 cut(s) | 1083 |
| BbvI | GCAGC | 7 cut(s) | 567, 613, 892, 1207, 1216, 1276, 1459 |
| BccI | CCATC | 6 cut(s) | 50, 305, 422, 466, 986, 1213 |
| BceAI | ACGGC | 3 cut(s) | 140, 406, 814 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1651, 1685 |
| BciT130I | CCWGG | 5 cut(s) | 113, 233, 251, 662, 1402 |
| BciVI | GTATCC | 1 cut(s) | 847 |
| BcnI | CCSGG | 4 cut(s) | 485, 885, 1343, 1411 |
| BcoDI | GTCTC | 3 cut(s) | 520, 843, 1133 |
| BfaI | CTAG | 3 cut(s) | 825, 954, 1622 |
| BfmI | CTRYAG | 1 cut(s) | 1230 |
| BfoI | RGCGCY | 1 cut(s) | 196 |
| BfuAI | ACCTGC | 2 cut(s) | 277, 787 |
| BfuI | GTATCC | 1 cut(s) | 847 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 80, 113, 221 |
| BmcAI | AGTACT | 2 cut(s) | 952, 1693 |
| Bme1390I | CCNGG | 9 cut(s) | 113, 233, 251, 485, 662, 885, 1343, 1402, 1411 |
| Bme18I | GGWCC | 8 cut(s) | 235, 253, 450, 487, 658, 963, 1421, 1593 |
| BmeT110I | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| BmgBI | CACGTC | 1 cut(s) | 220 |
| BmiI | GGNNCC | 4 cut(s) | 368, 488, 964, 1557 |
| BmrFI | CCNGG | 9 cut(s) | 113, 233, 251, 485, 662, 885, 1343, 1402, 1411 |
| BmsI | GCATC | 3 cut(s) | 132, 171, 216 |
| BpiI | GAAGAC | 2 cut(s) | 283, 597 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1083 |
| Bpu14I | TTCGAA | 1 cut(s) | 974 |
| BpuEI | CTTGAG | 3 cut(s) | 50, 569, 1052 |
| BpuMI | CCSGG | 4 cut(s) | 485, 885, 1343, 1411 |
| BsaAI | YACGTR | 1 cut(s) | 1651 |
| BsaHI | GRCGYC | 2 cut(s) | 276, 575 |
| BsaJI | CCNNGG | 3 cut(s) | 249, 306, 1401 |
| BsaWI | WCCGGW | 1 cut(s) | 537 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 119, 149 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 25, 52, 286, 496, 667, 884 |
| Bse118I | RCCGGY | 1 cut(s) | 1357 |
| Bse21I | CCTNAGG | 1 cut(s) | 1050 |
| Bse3DI | GCAATG | 2 cut(s) | 1476, 1643 |
| BseBI | CCWGG | 5 cut(s) | 113, 233, 251, 662, 1402 |
| BseDI | CCNNGG | 3 cut(s) | 249, 306, 1401 |
| BseGI | GGATG | 4 cut(s) | 10, 42, 871, 1462 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 25, 52, 286, 496, 667, 884 |
| BseMI | GCAATG | 2 cut(s) | 1476, 1643 |
| BseMII | CTCAG | 4 cut(s) | 428, 1041, 1074, 1458 |
| BsePI | GCGCGC | 1 cut(s) | 240 |
| BseRI | GAGGAG | 4 cut(s) | 38, 41, 326, 582 |
| BseX3I | CGGCCG | 2 cut(s) | 213, 309 |
| BseXI | GCAGC | 7 cut(s) | 567, 613, 892, 1207, 1216, 1276, 1459 |
| BseYI | CCCAGC | 3 cut(s) | 441, 893, 1438 |
| Bsh1236I | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| Bsh1285I | CGRYCG | 6 cut(s) | 130, 216, 312, 351, 538, 1265 |
| BshFI | GGCC | 8 cut(s) | 74, 83, 116, 215, 224, 311, 829, 1163 |
| BsiEI | CGRYCG | 6 cut(s) | 130, 216, 312, 351, 538, 1265 |
| BsiHKAI | GWGCWC | 2 cut(s) | 66, 1710 |
| BsiHKCI | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| BsiSI | CCGG | 8 cut(s) | 283, 484, 538, 884, 1286, 1343, 1358, 1411 |
| BslFI | GGGAC | 2 cut(s) | 500, 542 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 25, 52, 286, 496, 667, 884 |
| BsmAI | GTCTC | 3 cut(s) | 520, 843, 1133 |
| BsmBI | CGTCTC | 1 cut(s) | 520 |
| BsmFI | GGGAC | 2 cut(s) | 500, 542 |
| BsmI | GAATGC | 3 cut(s) | 640, 897, 1525 |
| BsnI | GGCC | 8 cut(s) | 74, 83, 116, 215, 224, 311, 829, 1163 |
| BsoBI | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| Bsp119I | TTCGAA | 1 cut(s) | 974 |
| Bsp1286I | GDGCHC | 3 cut(s) | 66, 1049, 1710 |
| Bsp143I | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| Bsp68I | TCGCGA | 2 cut(s) | 419, 461 |
| BspANI | GGCC | 8 cut(s) | 74, 83, 116, 215, 224, 311, 829, 1163 |
| BspCNI | CTCAG | 4 cut(s) | 429, 1042, 1075, 1459 |
| BspFNI | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| BspLI | GGNNCC | 4 cut(s) | 368, 488, 964, 1557 |
| BspMAI | CTGCAG | 1 cut(s) | 1234 |
| BspMI | ACCTGC | 2 cut(s) | 277, 787 |
| BspPI | GGATC | 3 cut(s) | 63, 144, 1353 |
| BspT104I | TTCGAA | 1 cut(s) | 974 |
| BsrDI | GCAATG | 2 cut(s) | 1476, 1643 |
| BsrFI | RCCGGY | 1 cut(s) | 1357 |
| BssAI | RCCGGY | 1 cut(s) | 1357 |
| BssECI | CCNNGG | 3 cut(s) | 249, 306, 1401 |
| BssHII | GCGCGC | 1 cut(s) | 240 |
| BssMI | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| BssNI | GRCGYC | 2 cut(s) | 276, 575 |
| BssSI | CACGAG | 1 cut(s) | 77 |
| Bst2BI | CACGAG | 1 cut(s) | 77 |
| Bst2UI | CCWGG | 5 cut(s) | 113, 233, 251, 662, 1402 |
| Bst4CI | ACNGT | 3 cut(s) | 679, 859, 1216 |
| Bst6I | CTCTTC | 1 cut(s) | 1326 |
| BstACI | GRCGYC | 2 cut(s) | 276, 575 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 623 |
| BstBAI | YACGTR | 1 cut(s) | 1651 |
| BstBI | TTCGAA | 1 cut(s) | 974 |
| BstC8I | GCNNGC | 5 cut(s) | 242, 266, 421, 522, 1010 |
| BstDEI | CTNAG | 5 cut(s) | 437, 1050, 1083, 1256, 1467 |
| BstDSI | CCRYGG | 1 cut(s) | 306 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 23, 284 |
| BstF5I | GGATG | 4 cut(s) | 10, 42, 871, 1462 |
| BstFNI | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| BstH2I | RGCGCY | 1 cut(s) | 196 |
| BstHHI | GCGC | 7 cut(s) | 107, 195, 242, 244, 395, 796, 1435 |
| BstKTI | GATC | 8 cut(s) | 58, 152, 351, 684, 1265, 1309, 1348, 1591 |
| BstMAI | GTCTC | 3 cut(s) | 520, 843, 1133 |
| BstMBI | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| BstMCI | CGRYCG | 6 cut(s) | 130, 216, 312, 351, 538, 1265 |
| BstNI | CCWGG | 5 cut(s) | 113, 233, 251, 662, 1402 |
| BstSCI | CCNGG | 9 cut(s) | 111, 231, 249, 483, 660, 883, 1341, 1400, 1409 |
| BstSFI | CTRYAG | 1 cut(s) | 1230 |
| BstUI | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| BstV1I | GCAGC | 7 cut(s) | 567, 613, 892, 1207, 1216, 1276, 1459 |
| BstV2I | GAAGAC | 2 cut(s) | 283, 597 |
| BstX2I | RGATCY | 1 cut(s) | 1345 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 232, 472, 1483 |
| BstYI | RGATCY | 1 cut(s) | 1345 |
| BstZI | CGGCCG | 2 cut(s) | 213, 309 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1050 |
| BsuI | GTATCC | 1 cut(s) | 847 |
| BsuRI | GGCC | 8 cut(s) | 74, 83, 116, 215, 224, 311, 829, 1163 |
| BtgI | CCRYGG | 1 cut(s) | 306 |
| BtgZI | GCGATG | 4 cut(s) | 148, 193, 400, 442 |
| BtrI | CACGTC | 1 cut(s) | 220 |
| BtsCI | GGATG | 4 cut(s) | 10, 42, 871, 1462 |
| BtsI | GCAGTG | 2 cut(s) | 1239, 1615 |
| BtsIMutI | CAGTG | 2 cut(s) | 1239, 1615 |
| BtuMI | TCGCGA | 2 cut(s) | 419, 461 |
| BveI | ACCTGC | 2 cut(s) | 277, 787 |
| Cac8I | GCNNGC | 5 cut(s) | 242, 266, 421, 522, 1010 |
| CfoI | GCGC | 7 cut(s) | 107, 195, 242, 244, 395, 796, 1435 |
| Cfr10I | RCCGGY | 1 cut(s) | 1357 |
| Cfr42I | CCGCGG | 1 cut(s) | 309 |
| CseI | GACGC | 3 cut(s) | 213, 265, 583 |
| Csp6I | GTAC | 2 cut(s) | 951, 1692 |
| CviAII | CATG | 1 cut(s) | 358 |
| CviQI | GTAC | 2 cut(s) | 951, 1692 |
| DdeI | CTNAG | 5 cut(s) | 437, 1050, 1083, 1256, 1467 |
| DpnI | GATC | 8 cut(s) | 57, 151, 350, 683, 1264, 1308, 1347, 1590 |
| DpnII | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| EaeI | YGGCCR | 4 cut(s) | 213, 222, 309, 1161 |
| EagI | CGGCCG | 2 cut(s) | 213, 309 |
| Eam1104I | CTCTTC | 1 cut(s) | 1326 |
| EarI | CTCTTC | 1 cut(s) | 1326 |
| EciI | GGCGGA | 4 cut(s) | 142, 626, 782, 791 |
| Ecl136II | GAGCTC | 1 cut(s) | 1708 |
| EclXI | CGGCCG | 2 cut(s) | 213, 309 |
| Eco24I | GRGCYC | 2 cut(s) | 1049, 1710 |
| Eco47I | GGWCC | 8 cut(s) | 235, 253, 450, 487, 658, 963, 1421, 1593 |
| Eco52I | CGGCCG | 2 cut(s) | 213, 309 |
| Eco53kI | GAGCTC | 1 cut(s) | 1708 |
| Eco57I | CTGAAG | 1 cut(s) | 614 |
| Eco81I | CCTNAGG | 1 cut(s) | 1050 |
| Eco88I | CYCGRG | 3 cut(s) | 98, 338, 1697 |
| EcoICRI | GAGCTC | 1 cut(s) | 1708 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 23, 284 |
| EcoO109I | RGGNCCY | 1 cut(s) | 963 |
| EcoRI | GAATTC | 1 cut(s) | 943 |
| EcoRII | CCWGG | 5 cut(s) | 111, 231, 249, 660, 1400 |
| EcoT38I | GRGCYC | 2 cut(s) | 1049, 1710 |
| Esp3I | CGTCTC | 1 cut(s) | 520 |
| FaeI | CATG | 1 cut(s) | 361 |
| FaiI | YATR | 6 cut(s) | 359, 648, 851, 1017, 1316, 1633 |
| FaqI | GGGAC | 2 cut(s) | 500, 542 |
| FatI | CATG | 1 cut(s) | 357 |
| FauI | CCCGC | 1 cut(s) | 1001 |
| FblI | GTMKAC | 1 cut(s) | 126 |
| FokI | GGATG | 4 cut(s) | 17, 29, 878, 1469 |
| FriOI | GRGCYC | 2 cut(s) | 1049, 1710 |
| FspBI | CTAG | 3 cut(s) | 825, 954, 1622 |
| FspI | TGCGCA | 1 cut(s) | 795 |
| GlaI | GCGC | 7 cut(s) | 106, 194, 241, 243, 394, 795, 1434 |
| GsaI | CCCAGC | 3 cut(s) | 445, 897, 1442 |
| HaeII | RGCGCY | 1 cut(s) | 196 |
| HaeIII | GGCC | 8 cut(s) | 74, 83, 116, 215, 224, 311, 829, 1163 |
| HapII | CCGG | 8 cut(s) | 283, 484, 538, 884, 1286, 1343, 1358, 1411 |
| HgaI | GACGC | 3 cut(s) | 213, 265, 583 |
| HhaI | GCGC | 7 cut(s) | 107, 195, 242, 244, 395, 796, 1435 |
| Hin1I | GRCGYC | 2 cut(s) | 276, 575 |
| Hin1II | CATG | 1 cut(s) | 361 |
| Hin6I | GCGC | 7 cut(s) | 105, 193, 240, 242, 393, 794, 1433 |
| HinP1I | GCGC | 7 cut(s) | 105, 193, 240, 242, 393, 794, 1433 |
| HincII | GTYRAC | 2 cut(s) | 127, 559 |
| HindII | GTYRAC | 2 cut(s) | 127, 559 |
| HindIII | AAGCTT | 1 cut(s) | 587 |
| HinfI | GANTC | 8 cut(s) | 86, 341, 383, 433, 697, 725, 976, 1241 |
| HpaII | CCGG | 8 cut(s) | 283, 484, 538, 884, 1286, 1343, 1358, 1411 |
| HphI | GGTGA | 3 cut(s) | 238, 292, 766 |
| Hpy166II | GTNNAC | 4 cut(s) | 127, 559, 802, 1174 |
| Hpy188III | TCNNGA | 8 cut(s) | 418, 460, 863, 1250, 1328, 1670, 1697, 1732 |
| Hpy8I | GTNNAC | 4 cut(s) | 127, 559, 802, 1174 |
| Hpy99I | CGWCG | 3 cut(s) | 207, 221, 349 |
| HpyAV | CCTTC | 3 cut(s) | 589, 1569, 1573 |
| HpyCH4III | ACNGT | 3 cut(s) | 679, 859, 1216 |
| HpyCH4IV | ACGT | 2 cut(s) | 219, 1650 |
| HpyCH4V | TGCA | 8 cut(s) | 264, 1195, 1232, 1292, 1351, 1481, 1615, 1648 |
| HpyF3I | CTNAG | 5 cut(s) | 437, 1050, 1083, 1256, 1467 |
| HpySE526I | ACGT | 2 cut(s) | 219, 1650 |
| Hsp92I | GRCGYC | 2 cut(s) | 276, 575 |
| Hsp92II | CATG | 1 cut(s) | 361 |
| HspAI | GCGC | 7 cut(s) | 105, 193, 240, 242, 393, 794, 1433 |
| KspI | CCGCGG | 1 cut(s) | 309 |
| Kzo9I | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| LmnI | GCTCC | 6 cut(s) | 28, 150, 372, 486, 778, 1705 |
| Lsp1109I | GCAGC | 7 cut(s) | 567, 613, 892, 1207, 1216, 1276, 1459 |
| LweI | GCATC | 3 cut(s) | 132, 171, 216 |
| MaeI | CTAG | 3 cut(s) | 825, 954, 1622 |
| MaeII | ACGT | 2 cut(s) | 219, 1650 |
| MaeIII | GTNAC | 5 cut(s) | 571, 820, 1132, 1377, 1634 |
| MalI | GATC | 8 cut(s) | 57, 151, 350, 683, 1264, 1308, 1347, 1590 |
| MboI | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| MboII | GAAGA | 4 cut(s) | 283, 559, 597, 1343 |
| MfeI | CAATTG | 1 cut(s) | 1371 |
| MflI | RGATCY | 1 cut(s) | 1345 |
| MhlI | GDGCHC | 3 cut(s) | 66, 1049, 1710 |
| MlsI | TGGCCA | 2 cut(s) | 224, 1163 |
| MluCI | AATT | 6 cut(s) | 552, 943, 1190, 1201, 1371, 1539 |
| MluNI | TGGCCA | 2 cut(s) | 224, 1163 |
| MlyI | GAGTC | 4 cut(s) | 95, 350, 392, 1250 |
| MmeI | TCCRAC | 2 cut(s) | 226, 366 |
| Mox20I | TGGCCA | 2 cut(s) | 224, 1163 |
| MscI | TGGCCA | 2 cut(s) | 224, 1163 |
| MseI | TTAA | 3 cut(s) | 915, 1200, 1272 |
| MslI | CAYNNNNRTG | 2 cut(s) | 470, 990 |
| Msp20I | TGGCCA | 2 cut(s) | 224, 1163 |
| MspA1I | CMGCKG | 3 cut(s) | 273, 308, 580 |
| MspI | CCGG | 8 cut(s) | 283, 484, 538, 884, 1286, 1343, 1358, 1411 |
| MspR9I | CCNGG | 9 cut(s) | 113, 233, 251, 485, 662, 885, 1343, 1402, 1411 |
| MunI | CAATTG | 1 cut(s) | 1371 |
| Mva1269I | GAATGC | 3 cut(s) | 640, 897, 1525 |
| MvaI | CCWGG | 5 cut(s) | 113, 233, 251, 662, 1402 |
| MvnI | CGCG | 9 cut(s) | 53, 212, 240, 242, 308, 395, 419, 461, 1435 |
| NciI | CCSGG | 4 cut(s) | 485, 885, 1343, 1411 |
| NdeII | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| NlaIII | CATG | 1 cut(s) | 361 |
| NlaIV | GGNNCC | 4 cut(s) | 368, 488, 964, 1557 |
| NmeAIII | GCCGAG | 1 cut(s) | 109 |
| NmuCI | GTSAC | 3 cut(s) | 571, 820, 1377 |
| NruI | TCGCGA | 2 cut(s) | 419, 461 |
| NsbI | TGCGCA | 1 cut(s) | 795 |
| NspV | TTCGAA | 1 cut(s) | 974 |
| PaeR7I | CTCGAG | 3 cut(s) | 98, 338, 1697 |
| PaqCI | CACCTGC | 1 cut(s) | 787 |
| PauI | GCGCGC | 1 cut(s) | 240 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 105, 195, 216, 392, 423, 1647 |
| PctI | GAATGC | 3 cut(s) | 640, 897, 1525 |
| PfeI | GAWTC | 4 cut(s) | 433, 697, 725, 976 |
| PfoI | TCCNGGA | 1 cut(s) | 483 |
| Ple19I | CGATCG | 2 cut(s) | 351, 1265 |
| PleI | GAGTC | 4 cut(s) | 94, 349, 391, 1249 |
| PpsI | GAGTC | 4 cut(s) | 94, 349, 391, 1249 |
| Ppu21I | YACGTR | 1 cut(s) | 1651 |
| PpuMI | RGGWCCY | 1 cut(s) | 963 |
| PshBI | ATTAAT | 1 cut(s) | 915 |
| Psp124BI | GAGCTC | 1 cut(s) | 1710 |
| Psp5II | RGGWCCY | 1 cut(s) | 963 |
| Psp6I | CCWGG | 5 cut(s) | 111, 231, 249, 660, 1400 |
| PspFI | CCCAGC | 3 cut(s) | 441, 893, 1438 |
| PspGI | CCWGG | 5 cut(s) | 111, 231, 249, 660, 1400 |
| PspN4I | GGNNCC | 4 cut(s) | 368, 488, 964, 1557 |
| PspPPI | RGGWCCY | 1 cut(s) | 963 |
| PspXI | VCTCGAGB | 2 cut(s) | 98, 338 |
| PstI | CTGCAG | 1 cut(s) | 1234 |
| PsuI | RGATCY | 1 cut(s) | 1345 |
| PteI | GCGCGC | 1 cut(s) | 240 |
| PvuI | CGATCG | 2 cut(s) | 351, 1265 |
| RruI | TCGCGA | 2 cut(s) | 419, 461 |
| RsaI | GTAC | 2 cut(s) | 952, 1693 |
| RsaNI | GTAC | 2 cut(s) | 951, 1692 |
| RseI | CAYNNNNRTG | 2 cut(s) | 470, 990 |
| SacI | GAGCTC | 1 cut(s) | 1710 |
| SacII | CCGCGG | 1 cut(s) | 309 |
| SalI | GTCGAC | 1 cut(s) | 125 |
| SaqAI | TTAA | 3 cut(s) | 915, 1200, 1272 |
| Sau3AI | GATC | 8 cut(s) | 55, 149, 348, 681, 1262, 1306, 1345, 1588 |
| ScaI | AGTACT | 2 cut(s) | 952, 1693 |
| SchI | GAGTC | 4 cut(s) | 95, 350, 392, 1250 |
| ScrFI | CCNGG | 9 cut(s) | 113, 233, 251, 485, 662, 885, 1343, 1402, 1411 |
| SduI | GDGCHC | 3 cut(s) | 66, 1049, 1710 |
| SfaNI | GCATC | 3 cut(s) | 132, 171, 216 |
| SfcI | CTRYAG | 1 cut(s) | 1230 |
| SfiI | GGCCNNNNNGGCC | 2 cut(s) | 80, 221 |
| Sfr274I | CTCGAG | 3 cut(s) | 98, 338, 1697 |
| Sfr303I | CCGCGG | 1 cut(s) | 309 |
| SfuI | TTCGAA | 1 cut(s) | 974 |
| SgrBI | CCGCGG | 1 cut(s) | 309 |
| SinI | GGWCC | 8 cut(s) | 235, 253, 450, 487, 658, 963, 1421, 1593 |
| SlaI | CTCGAG | 3 cut(s) | 98, 338, 1697 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 470, 990 |
| SmlI | CTYRAG | 6 cut(s) | 65, 98, 338, 584, 1067, 1697 |
| SmoI | CTYRAG | 6 cut(s) | 65, 98, 338, 584, 1067, 1697 |
| Sse9I | AATT | 6 cut(s) | 552, 943, 1190, 1201, 1371, 1539 |
| SspMI | CTAG | 3 cut(s) | 825, 954, 1622 |
| SstI | GAGCTC | 1 cut(s) | 1710 |
| StyD4I | CCNGG | 9 cut(s) | 111, 231, 249, 483, 660, 883, 1341, 1400, 1409 |
| TaaI | ACNGT | 3 cut(s) | 679, 859, 1216 |
| TaiI | ACGT | 2 cut(s) | 222, 1653 |
| TaqII | GACCGA | 1 cut(s) | 504 |
| TasI | AATT | 6 cut(s) | 552, 943, 1190, 1201, 1371, 1539 |
| TatI | WGTACW | 2 cut(s) | 950, 1691 |
| TfiI | GAWTC | 4 cut(s) | 433, 697, 725, 976 |
| Tru1I | TTAA | 3 cut(s) | 915, 1200, 1272 |
| Tru9I | TTAA | 3 cut(s) | 915, 1200, 1272 |
| TscAI | CASTG | 2 cut(s) | 1239, 1622 |
| TseFI | GTSAC | 3 cut(s) | 571, 820, 1377 |
| TseI | GCWGC | 7 cut(s) | 580, 626, 880, 1195, 1229, 1289, 1472 |
| Tsp45I | GTSAC | 3 cut(s) | 571, 820, 1377 |
| TspDTI | ATGAA | 3 cut(s) | 374, 635, 890 |
| TspGWI | ACGGA | 1 cut(s) | 671 |
| TspRI | CASTG | 2 cut(s) | 1239, 1622 |
| VpaK11BI | GGWCC | 8 cut(s) | 235, 253, 450, 487, 658, 963, 1421, 1593 |
| VspI | ATTAAT | 1 cut(s) | 915 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 23, 284 |
| XapI | RAATTY | 1 cut(s) | 943 |
| XhoI | CTCGAG | 3 cut(s) | 98, 338, 1697 |
| XmiI | GTMKAC | 1 cut(s) | 126 |
| XspI | CTAG | 3 cut(s) | 825, 954, 1622 |
| ZrmI | AGTACT | 2 cut(s) | 952, 1693 |