FvH4_5g19730

inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001702
Physical Location & Seq
Forward (+)
11557954 .. 11558690
737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19730.t1

Sequence Viewer

Length: 525 bp
ATGAAGATGAAGATGAATTTACTCAACTGGTCTGGAATAGTATTACTCGTGCTTATCATCAGCTGGGATCAGTCCCCAAAGTTTGCTTATGCTGATCTAATAGAAGATACATGTAACAAAACATCTTACGCCAAACTTTGTGTCTCAGTTTTAAGATCTAGTCCTCGTAGTGCTACTGCAGATGTGAAAGGGCTGGTTCAGATAGCGGTCGAATACTTGCAAGCGTACGTGAAAGACACACTTGCCCTATCAGAGAAGTTATTTAAGGAGGAAAAGAATCCATCTGTAAAAGGTGGCCTTAGACTTTGCGTTGATCAATATGCTTTGGGACTCCACCGCGTCGGCGACACCATCAACTTCTTAAACTCAGGTGATTATCGCAATGCACATGATATTGTTTTGACTGTAGCTGACGATGCGAGTACCTGTAATGACGCCGGTACAGGAACGCTGTTGAAGGATAGAAACAGTCAAGTAACCAACTCAACAAGACTGGCAATGGAGATGATTCAAATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

19.16

Weight (kDa)

6.72

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 166 1.1e-19 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 339
AciI CCGC 2 cut(s) 206, 337
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 2 cut(s) 16, 513
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 3 cut(s) 227, 424, 442
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 2 cut(s) 457, 512
AluBI AGCT 2 cut(s) 63, 410
AluI AGCT 2 cut(s) 63, 410
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 1 cut(s) 75
AoxI GGCC 1 cut(s) 295
ApoI RAATTY 2 cut(s) 16, 513
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AsuHPI GGTGA 1 cut(s) 383
BauI CACGAG 1 cut(s) 47
BccI CCATC 2 cut(s) 289, 359
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 2 cut(s) 177, 405
BglII AGATCT 1 cut(s) 155
BmsI GCATC 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 435
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 2 cut(s) 32, 498
Bse3DI GCAATG 2 cut(s) 388, 504
BseMI GCAATG 2 cut(s) 388, 504
BseMII CTCAG 2 cut(s) 159, 381
BseNI ACTGG 2 cut(s) 32, 498
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 339
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 438
BsiWI CGTACG 1 cut(s) 225
BslFI GGGAC 2 cut(s) 58, 342
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 2 cut(s) 58, 342
BsnI GGCC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 67, 94, 155, 313
BspACI CCGC 2 cut(s) 206, 337
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 2 cut(s) 158, 380
BspFNI CGCG 1 cut(s) 339
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 388, 504
BsrFI RCCGGY 1 cut(s) 437
BsrI ACTGG 2 cut(s) 32, 498
BssAI RCCGGY 1 cut(s) 437
BssMI GATC 4 cut(s) 67, 94, 155, 313
BssNI GRCGYC 1 cut(s) 435
BssSI CACGAG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 47
Bst4CI ACNGT 2 cut(s) 406, 470
BstACI GRCGYC 1 cut(s) 435
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 3 cut(s) 145, 299, 367
BstFNI CGCG 1 cut(s) 339
BstKTI GATC 4 cut(s) 70, 97, 158, 316
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 4 cut(s) 67, 94, 155, 313
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 114
BstSFI CTRYAG 2 cut(s) 177, 405
BstUI CGCG 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BsuRI GGCC 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 437
CseI GACGC 2 cut(s) 328, 443
Csp6I GTAC 3 cut(s) 226, 423, 441
CviAII CATG 2 cut(s) 111, 389
CviJI RGCY 4 cut(s) 63, 193, 297, 410
CviKI_1 RGCY 4 cut(s) 63, 193, 297, 410
CviQI GTAC 3 cut(s) 226, 423, 441
DdeI CTNAG 3 cut(s) 145, 299, 367
DpnI GATC 4 cut(s) 69, 96, 157, 315
DpnII GATC 4 cut(s) 67, 94, 155, 313
FaeI CATG 2 cut(s) 114, 392
FaiI YATR 4 cut(s) 90, 112, 321, 390
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 282, 314
FaqI GGGAC 2 cut(s) 58, 342
FatI CATG 2 cut(s) 110, 388
FbaI TGATCA 1 cut(s) 313
FspBI CTAG 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 67
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 438
HgaI GACGC 2 cut(s) 328, 443
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 2 cut(s) 114, 392
HinfI GANTC 3 cut(s) 277, 330, 508
HpaII CCGG 1 cut(s) 438
HphI GGTGA 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 201, 253
Hpy188III TCNNGA 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 2 cut(s) 406, 470
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 3 cut(s) 179, 220, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 3 cut(s) 145, 299, 367
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 2 cut(s) 114, 392
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 4 cut(s) 67, 94, 155, 313
LpnPI CCDG 9 cut(s) 13, 18, 49, 179, 354, 429, 439, 451, 479
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 113, 475
MalI GATC 4 cut(s) 69, 96, 157, 315
MboI GATC 4 cut(s) 67, 94, 155, 313
MboII GAAGA 3 cut(s) 16, 22, 116
MflI RGATCY 1 cut(s) 155
MluCI AATT 2 cut(s) 16, 513
MlyI GAGTC 1 cut(s) 324
MnlI CCTC 2 cut(s) 174, 262
MseI TTAA 3 cut(s) 152, 264, 362
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 416
NdeII GATC 4 cut(s) 67, 94, 155, 313
NlaIII CATG 2 cut(s) 114, 392
NspI RCATGY 1 cut(s) 114
PciI ACATGT 1 cut(s) 110
PfeI GAWTC 2 cut(s) 277, 508
Pfl23II CGTACG 1 cut(s) 225
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 229
PscI ACATGT 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 63
PspLI CGTACG 1 cut(s) 225
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 3 cut(s) 227, 424, 442
RsaNI GTAC 3 cut(s) 226, 423, 441
SaqAI TTAA 3 cut(s) 152, 264, 362
Sau3AI GATC 4 cut(s) 67, 94, 155, 313
SchI GAGTC 1 cut(s) 324
SetI ASST 6 cut(s) 65, 231, 295, 373, 412, 428
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 2 cut(s) 177, 405
Sse9I AATT 2 cut(s) 16, 513
SsiI CCGC 2 cut(s) 206, 337
SspMI CTAG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 406, 470
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 16, 513
TfiI GAWTC 2 cut(s) 277, 508
Tru1I TTAA 3 cut(s) 152, 264, 362
Tru9I TTAA 3 cut(s) 152, 264, 362
TspDTI ATGAA 3 cut(s) 17, 23, 29
XapI RAATTY 2 cut(s) 16, 513
XceI RCATGY 1 cut(s) 114
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.