RLG00000003263

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001702
Physical Location & Seq
Reverse (-)
46326235 .. 46327437
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003263

Sequence Viewer

Length: 681 bp
ATGCCAGGGGATTCCATTAACGGAGCTAGCATGGTCCTTAGCCCACCACCCAAGCATTATGATTTAGGTTATACATATCTCGGTCTGGTGGATGCACCTGATTTTTGTGAAACTGAGTTTAAAGAGAGGAAAACTCTGTCTCCTTTGAAGAAGGAGAACTATGTTCGAACAATTGGTCATCGTATCTCAAAATGTGCCGATGAATCCCACGTCTTTAGCTATGACCTATGCACAACCTCTCTCCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTAGCACTAAACAATGCAAGCAACACGGTTCAGATCATAAAAAATTATTTGTTGATGAGCAACGAAAGTCTAGATCCTTTTGTTGTGGTTTGCTTGAAGGACTGCCTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATGCTGAAGGAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTATGGAAGCATCGAGTACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGGAGTATTACCGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCACTGTGCATAGGCCACTTACTTACCTCGGGTTCTACCTTGTTGATGAGGTCCTCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

227

Amino Acids

24.61

Weight (kDa)

4.68

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 72 - 209 2.1e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 371, 437
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 3 cut(s) 148, 400, 602
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 4
AluBI AGCT 4 cut(s) 26, 219, 304, 415
AluI AGCT 4 cut(s) 26, 219, 304, 415
Alw26I GTCTC 2 cut(s) 144, 573
AlwI GGATC 2 cut(s) 371, 437
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 1 cut(s) 628
ApeKI GCWGC 1 cut(s) 412
ArsI GACNNNNNNTTYG 2 cut(s) 160, 192
AspS9I GGNCC 2 cut(s) 34, 666
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 166
AsuNHI GCTAGC 2 cut(s) 26, 304
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 2 cut(s) 34, 666
BbvI GCAGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 144, 573
BfaI CTAG 4 cut(s) 27, 284, 305, 374
BfmI CTRYAG 1 cut(s) 410
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
Bme1390I CCNGG 1 cut(s) 6
Bme18I GGWCC 2 cut(s) 34, 666
BmeT110I CYCGRG 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 34, 666
BmrFI CCNGG 1 cut(s) 6
BmsI GCATC 3 cut(s) 82, 436, 524
BmtI GCTAGC 2 cut(s) 30, 308
BplI GAGNNNNNCTC 2 cut(s) 118, 150
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 1 cut(s) 38
Bpu14I TTCGAA 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 5, 277, 642
BsaWI WCCGGW 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bse1I ACTGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 5, 277, 642
BseGI GGATG 2 cut(s) 97, 451
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 144, 573
BsmBI CGTCTC 1 cut(s) 573
BsnI GGCC 1 cut(s) 630
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 166
Bsp143I GATC 3 cut(s) 336, 376, 429
BspANI GGCC 1 cut(s) 630
BspCNI CTCAG 1 cut(s) 106
BspMAI CTGCAG 1 cut(s) 414
BspOI GCTAGC 2 cut(s) 30, 308
BspPI GGATC 2 cut(s) 371, 437
BspT104I TTCGAA 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 1 cut(s) 444
BssECI CCNNGG 3 cut(s) 5, 277, 642
BssMI GATC 3 cut(s) 336, 376, 429
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 6
Bst4CI ACNGT 3 cut(s) 331, 567, 621
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 4 cut(s) 28, 306, 322, 410
BstDEI CTNAG 3 cut(s) 38, 114, 497
BstF5I GGATG 2 cut(s) 97, 451
BstKTI GATC 3 cut(s) 339, 379, 432
BstMAI GTCTC 2 cut(s) 144, 573
BstMBI GATC 3 cut(s) 336, 376, 429
BstMWI GCNNNNNNNGC 2 cut(s) 293, 455
BstNI CCWGG 1 cut(s) 6
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 1 cut(s) 4
BstSFI CTRYAG 1 cut(s) 410
BstV1I GCAGC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 630
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 97, 451
BtsIMutI CAGTG 2 cut(s) 437, 617
Cac8I GCNNGC 4 cut(s) 28, 306, 322, 410
Cfr13I GGNCC 2 cut(s) 34, 666
Csp6I GTAC 1 cut(s) 522
CviAII CATG 3 cut(s) 31, 263, 525
CviJI RGCY 9 cut(s) 26, 42, 219, 261, 269, 304, 415, 537, 630
CviKI_1 RGCY 9 cut(s) 26, 42, 219, 261, 269, 304, 415, 537, 630
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 3 cut(s) 38, 114, 497
DpnI GATC 3 cut(s) 338, 378, 431
DpnII GATC 3 cut(s) 336, 376, 429
DraI TTTAAA 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 34, 666
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 666
EcoRII CCWGG 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 3 cut(s) 34, 266, 528
FatI CATG 3 cut(s) 30, 262, 524
Fnu4HI GCNGC 1 cut(s) 413
FokI GGATG 2 cut(s) 104, 458
Fsp4HI GCNGC 1 cut(s) 413
FspBI CTAG 4 cut(s) 27, 284, 305, 374
GluI GCNGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 1 cut(s) 630
HapII CCGG 1 cut(s) 254
Hin1II CATG 3 cut(s) 34, 266, 528
HinfI GANTC 2 cut(s) 11, 203
HpaII CCGG 1 cut(s) 254
HphI GGTGA 1 cut(s) 479
Hpy188I TCNGA 2 cut(s) 336, 424
Hpy188III TCNNGA 1 cut(s) 374
HpyAV CCTTC 6 cut(s) 145, 394, 446, 527, 541, 545
HpyCH4III ACNGT 3 cut(s) 331, 567, 621
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 8 cut(s) 95, 231, 320, 412, 503, 617, 624, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 455
HpyF3I CTNAG 3 cut(s) 38, 114, 497
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 3 cut(s) 34, 266, 528
Kzo9I GATC 3 cut(s) 336, 376, 429
LmnI GCTCC 3 cut(s) 23, 301, 455
LpnPI CCDG 7 cut(s) 18, 71, 111, 267, 422, 425, 449
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 3 cut(s) 82, 436, 524
MaeI CTAG 4 cut(s) 27, 284, 305, 374
MaeII ACGT 1 cut(s) 210
MalI GATC 3 cut(s) 338, 378, 431
MboI GATC 3 cut(s) 336, 376, 429
MboII GAAGA 1 cut(s) 160
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 376
MluCI AATT 4 cut(s) 171, 346, 586, 602
MnlI CCTC 7 cut(s) 120, 247, 436, 523, 652, 657, 679
MseI TTAA 4 cut(s) 18, 120, 540, 679
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 6
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 293, 455
NdeII GATC 3 cut(s) 336, 376, 429
NheI GCTAGC 2 cut(s) 26, 304
NlaIII CATG 3 cut(s) 34, 266, 528
NspI RCATGY 1 cut(s) 528
NspV TTCGAA 1 cut(s) 166
PciI ACATGT 1 cut(s) 524
PfeI GAWTC 2 cut(s) 11, 203
PkrI GCNGC 1 cut(s) 414
PpuMI RGGWCCY 1 cut(s) 666
PscI ACATGT 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PspPI GGNCC 2 cut(s) 34, 666
PspPPI RGGWCCY 1 cut(s) 666
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
SaqAI TTAA 4 cut(s) 18, 120, 540, 679
SatI GCNGC 1 cut(s) 413
Sau3AI GATC 3 cut(s) 336, 376, 429
Sau96I GGNCC 2 cut(s) 34, 666
ScrFI CCNGG 1 cut(s) 6
SfaNI GCATC 3 cut(s) 82, 436, 524
SfcI CTRYAG 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 166
SinI GGWCC 2 cut(s) 34, 666
Sse9I AATT 4 cut(s) 171, 346, 586, 602
SspI AATATT 1 cut(s) 490
SspMI CTAG 4 cut(s) 27, 284, 305, 374
StyD4I CCNGG 1 cut(s) 4
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 331, 567, 621
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 166, 518
TaqII GACCGA 1 cut(s) 71
TasI AATT 4 cut(s) 171, 346, 586, 602
TatI WGTACW 1 cut(s) 521
TfiI GAWTC 2 cut(s) 11, 203
Tru1I TTAA 4 cut(s) 18, 120, 540, 679
Tru9I TTAA 4 cut(s) 18, 120, 540, 679
TscAI CASTG 2 cut(s) 444, 624
TseI GCWGC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 2 cut(s) 444, 624
VpaK11BI GGWCC 2 cut(s) 34, 666
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 1 cut(s) 528
XspI CTAG 4 cut(s) 27, 284, 305, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.