Rroxscaffold_4G00310090

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001702
Physical Location & Seq
Forward (+)
32016791 .. 32017497
707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310090.1

Sequence Viewer

Length: 585 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTCTCTTTCTCCTCATCTCATCAACCTTCACTCCCATAACTGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCGGAACGACCCCAACCTGAGCTACAAGTTCTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCACCGCGCCGATCTCCGGCGACTAGGCCTCATCTCCATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCACTTCATCAAGCATCTTCTCAAGAACAAGAAGCTTGACAAGTTCGTAAGGGCGCGTTTGAATGACTGTTTGGAGCTTTACACAGAAGCCATACCCACCATCAAGCAAGCCATCAAGGATTACAAAAAGAAGCACAACAAAGACGCCAATATCAAAGTGAGTTCGATCGTCGATGCCTCCACGACTTGCGAAGATGGATTCGAGGAGAGACCAGGGTTGGTTTCGCCATTAACGAAAAGGAACAAAGAGACGTTTCAGCTGTGTATTATAGCGCTTTCGATTATCAATATGGTTGTCTATGATCGAACTCAGGTAGCTGTAACTGCTGTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

194

Amino Acids

21.98

Weight (kDa)

9.59

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 177 2.3e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccII CGCG 2 cut(s) 183, 304
AciI CCGC 2 cut(s) 165, 181
AcyI GRCGYC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 235
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AgsI TTSAA 1 cut(s) 310
AjnI CCWGG 1 cut(s) 460
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 6 cut(s) 135, 220, 283, 325, 508, 566
AluI AGCT 6 cut(s) 135, 220, 283, 325, 508, 566
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 451, 491
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 202
AspLEI GCGC 3 cut(s) 185, 304, 523
AsuHPI GGTGA 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 3 cut(s) 356, 368, 437
BciT130I CCWGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 451, 491
BfaI CTAG 2 cut(s) 200, 221
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 271, 412
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 2 cut(s) 122, 572
BseRI GAGGAG 2 cut(s) 35, 467
Bsh1236I CGCG 2 cut(s) 183, 304
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 2 cut(s) 451, 491
BsmBI CGTCTC 1 cut(s) 491
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 3 cut(s) 187, 414, 550
BspACI CCGC 2 cut(s) 165, 181
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 123, 571
BspFNI CGCG 2 cut(s) 183, 304
BspMI ACCTGC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 3 cut(s) 187, 414, 550
BssNI GRCGYC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 2 cut(s) 131, 558
BstFNI CGCG 2 cut(s) 183, 304
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 3 cut(s) 185, 304, 523
BstKTI GATC 3 cut(s) 190, 417, 553
BstMAI GTCTC 2 cut(s) 451, 491
BstMBI GATC 3 cut(s) 187, 414, 550
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 2 cut(s) 226, 572
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 2 cut(s) 183, 304
BsuRI GGCC 1 cut(s) 204
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 357
CfoI GCGC 3 cut(s) 185, 304, 523
CseI GACGC 1 cut(s) 401
CviAII CATG 1 cut(s) 214
DdeI CTNAG 2 cut(s) 131, 558
DpnI GATC 3 cut(s) 189, 416, 552
DpnII GATC 3 cut(s) 187, 414, 550
DraIII CACNNNGTG 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 1 cut(s) 217
FaiI YATR 8 cut(s) 14, 71, 215, 341, 518, 539, 549, 583
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 200, 221
GlaI GCGC 3 cut(s) 184, 303, 522
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 401
HhaI GCGC 3 cut(s) 185, 304, 523
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 3 cut(s) 183, 302, 521
HinP1I GCGC 3 cut(s) 183, 302, 521
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 1 cut(s) 447
HpaII CCGG 1 cut(s) 193
HphI GGTGA 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 94, 117
Hpy188III TCNNGA 1 cut(s) 271
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 31, 70
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 234, 500
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 572
HpyF3I CTNAG 2 cut(s) 131, 558
HpySE526I ACGT 2 cut(s) 234, 500
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 3 cut(s) 183, 302, 521
Kzo9I GATC 3 cut(s) 187, 414, 550
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 7 cut(s) 112, 143, 206, 239, 447, 474, 545
LweI GCATC 2 cut(s) 271, 412
MaeI CTAG 2 cut(s) 200, 221
MaeII ACGT 2 cut(s) 234, 500
MaeIII GTNAC 4 cut(s) 148, 235, 568, 577
MalI GATC 3 cut(s) 189, 416, 552
MboI GATC 3 cut(s) 187, 414, 550
MboII GAAGA 2 cut(s) 257, 452
MhlI GDGCHC 1 cut(s) 115
MnlI CCTC 6 cut(s) 41, 45, 56, 215, 436, 445
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 2 cut(s) 183, 304
MwoI GCNNNNNNNGC 2 cut(s) 226, 572
NdeII GATC 3 cut(s) 187, 414, 550
NlaIII CATG 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 148, 235
PceI AGGCCT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 166
Ple19I CGATCG 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PvuI CGATCG 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 3 cut(s) 187, 414, 550
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 412
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 2 cut(s) 165, 181
SspMI CTAG 2 cut(s) 200, 221
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 237, 503
TaqI TCGA 5 cut(s) 413, 420, 450, 527, 553
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TseFI GTSAC 2 cut(s) 148, 235
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 2 cut(s) 230, 244
XspI CTAG 2 cut(s) 200, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.