Rh1DG186000

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001702
Physical Location & Seq
Reverse (-)
37423746 .. 37424528
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG186000.1

Sequence Viewer

Length: 405 bp
ATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCGCTTCATCAAGCATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGATGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCTAGCATCAAGCAAGCCGTCAAGGATTACAAAATGAAGGACAATAGACACGCCAATATCGAAGTGAGTTCGGTCACCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACAACGAGATGTTTCAGCTGTGTGTTATAGCGCTTAGGATTATCAATATGTGTTATGGACGACTATCAGTTGTTGATCCAACTTTCGTGACAGGGCTTATTGACAATCTAAATGTATGCAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

134

Amino Acids

15.31

Weight (kDa)

7.63

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 106 1.6e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AciI CCGC 1 cut(s) 213
AclWI GGATC 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 22
AfeI AGCGCT 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 33
AgeI ACCGGT 1 cut(s) 206
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 3 cut(s) 7, 70, 295
AluI AGCT 3 cut(s) 7, 70, 295
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 1 cut(s) 347
Aor51HI AGCGCT 1 cut(s) 309
AsiGI ACCGGT 1 cut(s) 206
AspLEI GCGC 2 cut(s) 39, 310
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 237
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 209
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 2 cut(s) 8, 132
BfoI RGCGCY 2 cut(s) 40, 311
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 58, 144
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 206
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 2 cut(s) 93, 103
BseLI CCNNNNNNNGG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 209
BshTI ACCGGT 1 cut(s) 206
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 238
Bso31I GGTCTC 1 cut(s) 238
Bsp119I TTCGAA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 211
BspPI GGATC 1 cut(s) 347
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 2 cut(s) 234, 266
BstACI GRCGYC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 1 cut(s) 311
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 2 cut(s) 93, 103
BstH2I RGCGCY 2 cut(s) 40, 311
BstHHI GCGC 2 cut(s) 39, 310
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNI CCWGG 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 93, 103
Cac8I GCNNGC 1 cut(s) 144
CfoI GCGC 2 cut(s) 39, 310
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 131
CspAI ACCGGT 1 cut(s) 206
CviJI RGCY 5 cut(s) 7, 70, 146, 295, 373
CviKI_1 RGCY 5 cut(s) 7, 70, 146, 295, 373
DdeI CTNAG 1 cut(s) 311
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DraIII CACNNNGTG 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 234, 266
EarI CTCTTC 2 cut(s) 234, 266
Eco31I GGTCTC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 109
Eco47III AGCGCT 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 247
FaiI YATR 5 cut(s) 128, 305, 326, 333, 394
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 2 cut(s) 100, 110
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 2 cut(s) 8, 132
GlaI GCGC 2 cut(s) 38, 309
GluI GCNGC 1 cut(s) 213
HaeII RGCGCY 2 cut(s) 40, 311
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 131
HhaI GCGC 2 cut(s) 39, 310
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 2 cut(s) 37, 308
HinP1I GCGC 2 cut(s) 37, 308
HinfI GANTC 1 cut(s) 234
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 196
Hpy188III TCNNGA 3 cut(s) 58, 364, 402
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 2 cut(s) 37, 308
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 5 cut(s) 26, 220, 234, 261, 354
LweI GCATC 2 cut(s) 58, 144
MaeI CTAG 2 cut(s) 8, 132
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 22, 202, 364
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 4 cut(s) 44, 239, 251, 283
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 2 cut(s) 58, 267
MseI TTAA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 22, 202, 364
NspV TTCGAA 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 266
PfeI GAWTC 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 295
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 249
SetI ASST 6 cut(s) 9, 24, 72, 114, 133, 297
SfaNI GCATC 2 cut(s) 58, 144
SfuI TTCGAA 1 cut(s) 237
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SsiI CCGC 1 cut(s) 213
SspMI CTAG 2 cut(s) 8, 132
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 189, 237
TaqII GACCGA 1 cut(s) 190
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseFI GTSAC 3 cut(s) 22, 202, 364
Tsp45I GTSAC 3 cut(s) 22, 202, 364
TspDTI ATGAA 3 cut(s) 17, 31, 179
VpaK11BI GGWCC 1 cut(s) 109
XspI CTAG 2 cut(s) 8, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.