Rroxscaffold_4G00310110

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001702
Physical Location & Seq
Forward (+)
32023651 .. 32024348
698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310110.1

Sequence Viewer

Length: 609 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTTTCTTCCTCCTCTTCTCATCAACCTTCACTCCCATAACTGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCAAAAAGACCCCAACCTGAGCTACAAGTTCTGCGTGACTTCTCTCCAAGCCGACCCAAACAGCCAAGACGCCAATCTCCGGCAACTCGGCCTCATCTCCATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCGCTTCATCAAGCATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGGTGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCCAGCATCAAGCAAGCCGTCCAGGATTACAAAATGAAGGACAACAGACACGCCAATATCGCAGTGAGTTCGGTCGCCGGTGCTGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACCACGAGATGTTTCAGCTGTGTGTTATAGCGCTTATGATTATCAATATGGCTGTTTCTGATCGAACTCGGCTAGCATGTAACTTGTTTGAGGCTGTTTCTGACAACAATCTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

202

Amino Acids

22.8

Weight (kDa)

6.88

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 177 4.7e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AcyI GRCGYC 2 cut(s) 183, 336
AdeI CACNNNGTG 1 cut(s) 235
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AjnI CCWGG 2 cut(s) 363, 460
AluBI AGCT 4 cut(s) 135, 220, 283, 508
AluI AGCT 4 cut(s) 135, 220, 283, 508
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 451
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 425
AspLEI GCGC 2 cut(s) 252, 523
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 450
AsuNHI GCTAGC 1 cut(s) 562
AvaII GGWCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 412
BccI CCATC 1 cut(s) 437
BceAI ACGGC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 2 cut(s) 365, 462
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 221, 563
BfoI RGCGCY 2 cut(s) 253, 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 2 cut(s) 365, 462
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 491
BmrFI CCNGG 2 cut(s) 365, 462
BmsI GCATC 2 cut(s) 271, 357
BmtI GCTAGC 1 cut(s) 566
Bpu10I CCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 183, 336
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
Bse118I RCCGGY 1 cut(s) 419
BseBI CCWGG 2 cut(s) 365, 462
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 2 cut(s) 35, 293
BseXI GCAGC 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 193, 420
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 451
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 1 cut(s) 550
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 491
BspMI ACCTGC 1 cut(s) 107
BspOI GCTAGC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 1 cut(s) 550
BssNI GRCGYC 2 cut(s) 183, 336
BssSI CACGAG 1 cut(s) 494
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 2 cut(s) 365, 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 3 cut(s) 53, 447, 479
BstACI GRCGYC 2 cut(s) 183, 336
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 2 cut(s) 357, 564
BstDEI CTNAG 2 cut(s) 131, 602
BstF5I GGATG 1 cut(s) 316
BstH2I RGCGCY 2 cut(s) 253, 524
BstHHI GCGC 2 cut(s) 252, 523
BstKTI GATC 1 cut(s) 553
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 1 cut(s) 550
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 3 cut(s) 226, 401, 425
BstNI CCWGG 2 cut(s) 365, 462
BstNSI RCATGY 1 cut(s) 570
BstSCI CCNGG 2 cut(s) 363, 460
BstV1I GCAGC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 2 cut(s) 357, 564
CfoI GCGC 2 cut(s) 252, 523
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 322
CseI GACGC 2 cut(s) 191, 344
CviAII CATG 2 cut(s) 214, 567
DdeI CTNAG 2 cut(s) 131, 602
DpnI GATC 1 cut(s) 552
DpnII GATC 1 cut(s) 550
DraIII CACNNNGTG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 53, 447, 479
EarI CTCTTC 3 cut(s) 53, 447, 479
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 322
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 2 cut(s) 363, 460
FaeI CATG 2 cut(s) 217, 570
FaiI YATR 8 cut(s) 14, 71, 215, 341, 518, 527, 539, 568
FatI CATG 2 cut(s) 213, 566
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 2 cut(s) 221, 563
GlaI GCGC 2 cut(s) 251, 522
GluI GCNGC 1 cut(s) 426
GsaI CCCAGC 1 cut(s) 348
HaeII RGCGCY 2 cut(s) 253, 524
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 193, 420
HgaI GACGC 2 cut(s) 191, 344
HhaI GCGC 2 cut(s) 252, 523
Hin1I GRCGYC 2 cut(s) 183, 336
Hin1II CATG 2 cut(s) 217, 570
Hin6I GCGC 2 cut(s) 250, 521
HinP1I GCGC 2 cut(s) 250, 521
HinfI GANTC 1 cut(s) 447
HpaII CCGG 2 cut(s) 193, 420
HphI GGTGA 1 cut(s) 10
Hpy188I TCNGA 3 cut(s) 94, 550, 592
Hpy188III TCNNGA 1 cut(s) 271
HpyAV CCTTC 3 cut(s) 31, 70, 373
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 401, 425
HpyF3I CTNAG 2 cut(s) 131, 602
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 2 cut(s) 183, 336
Hsp92II CATG 2 cut(s) 217, 570
HspAI GCGC 2 cut(s) 250, 521
Kzo9I GATC 1 cut(s) 550
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 271, 357
MaeI CTAG 2 cut(s) 221, 563
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 148, 235, 569
MalI GATC 1 cut(s) 552
MboI GATC 1 cut(s) 550
MboII GAAGA 6 cut(s) 31, 40, 257, 452, 464, 496
MhlI GDGCHC 1 cut(s) 115
MnlI CCTC 7 cut(s) 41, 53, 56, 215, 271, 480, 574
MseI TTAA 2 cut(s) 479, 607
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 2 cut(s) 193, 420
MspR9I CCNGG 2 cut(s) 365, 462
MvaI CCWGG 2 cut(s) 365, 462
MwoI GCNNNNNNNGC 3 cut(s) 226, 401, 425
NdeII GATC 1 cut(s) 550
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 2 cut(s) 217, 570
NlaIV GGNNCC 1 cut(s) 491
NmeAIII GCCGAG 2 cut(s) 180, 538
NmuCI GTSAC 2 cut(s) 148, 235
NspI RCATGY 1 cut(s) 570
NspV TTCGAA 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PfeI GAWTC 1 cut(s) 447
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 363, 460
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 2 cut(s) 363, 460
PspN4I GGNNCC 1 cut(s) 491
PspPI GGNCC 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 479, 607
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 1 cut(s) 550
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 2 cut(s) 365, 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 357
SfuI TTCGAA 1 cut(s) 450
SgrAI CRCCGGYG 1 cut(s) 419
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SspMI CTAG 2 cut(s) 221, 563
StyD4I CCNGG 2 cut(s) 363, 460
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 450, 553
TaqII GACCGA 1 cut(s) 403
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 2 cut(s) 479, 607
Tru9I TTAA 2 cut(s) 479, 607
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 148, 235
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 3 cut(s) 230, 244, 392
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 322
XceI RCATGY 1 cut(s) 570
XspI CTAG 2 cut(s) 221, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.