FvH4_5g20950

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001702
Physical Location & Seq
Forward (+)
12602809 .. 12603796
988 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20950.t1

Sequence Viewer

Length: 672 bp
ATGAAAATCACATTAGAACTACATATATTCCACTACATGAGCAAGAAACCAAAGGAAACAATGTACCATATTTCTTTATTTTCAATCTCTCTATGCTGCTGCTTCTTTTTCCTTGTAATTCCTACTGAAAATTTCTCAACAGTTACTGCAATATCTGCCGATATAATCAATCATACCTGCAGAAAATGCGCCGATGAATCACACGTCTTTAGCTACGATCTATGCACAACCTCTCTCCAAACAGTTCCGGTGAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTGGCACTGAACAATGCAAGCAATACGCTTCTGATCATACAGAATTTGTTGACGAGCAACGAAAGTCTAGATCCTTTTGCTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCGGACGGCATCAGAACCTTGGTCGATGCTGTTGGAGCATTTCTAGGTGAGCAATATGACAACGCCAATATCTGGCTGAGTGCAGTTATGCAAGCATCAGATACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGAAGTGTTACCGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCACTGTGCGTAGGGCACCTGCTTACCTCAGATTCTACCATGTTGATGAGATCCTCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

224

Amino Acids

24.68

Weight (kDa)

4.74

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 55 - 205 6.9e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 633
Acc36I ACCTGC 2 cut(s) 185, 633
AccB1I GGYRCC 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 483
AclWI GGATC 2 cut(s) 362, 651
AcsI RAATTY 2 cut(s) 130, 340
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 483
AflIII ACRYGT 2 cut(s) 202, 515
AgsI TTSAA 3 cut(s) 84, 391, 593
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 3 cut(s) 213, 298, 406
AluI AGCT 3 cut(s) 213, 298, 406
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 2 cut(s) 362, 651
AlwNI CAGNNNCTG 1 cut(s) 146
ApeKI GCWGC 3 cut(s) 96, 99, 403
ApoI RAATTY 2 cut(s) 130, 340
AspLEI GCGC 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 262, 470, 557
BaeGI GKGCMC 1 cut(s) 624
BanI GGYRCC 1 cut(s) 621
BbvI GCAGC 3 cut(s) 83, 86, 415
BceAI ACGGC 1 cut(s) 433
BclI TGATCA 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 3 cut(s) 278, 365, 455
BfmI CTRYAG 1 cut(s) 178
BfuAI ACCTGC 2 cut(s) 185, 633
BisI GCNGC 3 cut(s) 97, 100, 404
BlsI GCNGC 3 cut(s) 98, 101, 405
BmgBI CACGTC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 623
BmsI GCATC 3 cut(s) 427, 429, 515
BsaJI CCNNGG 2 cut(s) 271, 429
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 271, 429
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 2 cut(s) 479, 648
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 3 cut(s) 83, 86, 415
BsgI GTGCAG 1 cut(s) 513
BshNI GGYRCC 1 cut(s) 621
BsiSI CCGG 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 564
BsmBI CGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 624
Bsp143I GATC 4 cut(s) 217, 330, 367, 656
BspCNI CTCAG 2 cut(s) 480, 647
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 1 cut(s) 182
BspMI ACCTGC 2 cut(s) 185, 633
BspPI GGATC 2 cut(s) 362, 651
BspT107I GGYRCC 1 cut(s) 621
BsrDI GCAATG 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 271, 429
BssMI GATC 4 cut(s) 217, 330, 367, 656
BssT1I CCWWGG 2 cut(s) 271, 429
Bst4CI ACNGT 4 cut(s) 142, 244, 558, 612
BstAPI GCANNNNNTGC 2 cut(s) 155, 186
BstC8I GCNNGC 4 cut(s) 300, 316, 401, 504
BstDEI CTNAG 2 cut(s) 488, 634
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 4 cut(s) 220, 333, 370, 659
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 4 cut(s) 217, 330, 367, 656
BstMWI GCNNNNNNNGC 4 cut(s) 155, 186, 287, 446
BstNSI RCATGY 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 624
BstV1I GCAGC 3 cut(s) 83, 86, 415
BstX2I RGATCY 2 cut(s) 367, 656
BstYI RGATCY 2 cut(s) 367, 656
BtrI CACGTC 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 302, 608
BveI ACCTGC 2 cut(s) 185, 633
Cac8I GCNNGC 4 cut(s) 300, 316, 401, 504
CaiI CAGNNNCTG 1 cut(s) 146
CfoI GCGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 64
CviAII CATG 4 cut(s) 37, 257, 516, 646
CviJI RGCY 7 cut(s) 213, 255, 263, 298, 406, 487, 528
CviKI_1 RGCY 7 cut(s) 213, 255, 263, 298, 406, 487, 528
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 2 cut(s) 488, 634
DpnI GATC 4 cut(s) 219, 332, 369, 658
DpnII GATC 4 cut(s) 217, 330, 367, 656
Eco130I CCWWGG 2 cut(s) 271, 429
EcoT14I CCWWGG 2 cut(s) 271, 429
ErhI CCWWGG 2 cut(s) 271, 429
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 4 cut(s) 40, 260, 519, 649
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 4 cut(s) 36, 256, 515, 645
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 3 cut(s) 97, 100, 404
Fsp4HI GCNGC 3 cut(s) 97, 100, 404
FspBI CTAG 3 cut(s) 278, 365, 455
GlaI GCGC 1 cut(s) 190
GluI GCNGC 3 cut(s) 97, 100, 404
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 191
Hin1II CATG 4 cut(s) 40, 260, 519, 649
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 2 cut(s) 197, 638
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 262, 470, 557
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 5 cut(s) 330, 415, 425, 511, 637
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 1 cut(s) 348
HpyAV CCTTC 4 cut(s) 385, 518, 532, 536
HpyCH4III ACNGT 4 cut(s) 142, 244, 558, 612
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 9 cut(s) 149, 180, 225, 314, 403, 494, 502, 608, 665
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 186, 287, 446
HpyF3I CTNAG 2 cut(s) 488, 634
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 4 cut(s) 40, 260, 519, 649
HspAI GCGC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 4 cut(s) 217, 330, 367, 656
LmnI GCTCC 2 cut(s) 295, 446
LpnPI CCDG 5 cut(s) 190, 261, 284, 469, 638
Lsp1109I GCAGC 3 cut(s) 83, 86, 415
LweI GCATC 3 cut(s) 427, 429, 515
MaeI CTAG 3 cut(s) 278, 365, 455
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 2 cut(s) 142, 552
MalI GATC 4 cut(s) 219, 332, 369, 658
MboI GATC 4 cut(s) 217, 330, 367, 656
MflI RGATCY 2 cut(s) 367, 656
MhlI GDGCHC 1 cut(s) 624
MluCI AATT 5 cut(s) 117, 130, 340, 577, 593
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 4 cut(s) 241, 514, 643, 670
MseI TTAA 2 cut(s) 531, 670
MslI CAYNNNNRTG 1 cut(s) 650
MspI CCGG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 155, 186, 287, 446
NdeII GATC 4 cut(s) 217, 330, 367, 656
NlaIII CATG 4 cut(s) 40, 260, 519, 649
NlaIV GGNNCC 1 cut(s) 623
NspI RCATGY 1 cut(s) 519
PaqCI CACCTGC 1 cut(s) 633
PciI ACATGT 1 cut(s) 515
PfeI GAWTC 2 cut(s) 197, 638
PflMI CCANNNNNTGG 1 cut(s) 483
PkrI GCNGC 3 cut(s) 98, 101, 405
PscI ACATGT 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 623
PstI CTGCAG 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 2 cut(s) 367, 656
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 2 cut(s) 531, 670
SatI GCNGC 3 cut(s) 97, 100, 404
Sau3AI GATC 4 cut(s) 217, 330, 367, 656
SduI GDGCHC 1 cut(s) 624
SfaNI GCATC 3 cut(s) 427, 429, 515
SfcI CTRYAG 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 650
Sse9I AATT 5 cut(s) 117, 130, 340, 577, 593
SspMI CTAG 3 cut(s) 278, 365, 455
StyI CCWWGG 2 cut(s) 271, 429
TaaI ACNGT 4 cut(s) 142, 244, 558, 612
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 435
TasI AATT 5 cut(s) 117, 130, 340, 577, 593
TfiI GAWTC 2 cut(s) 197, 638
Tru1I TTAA 2 cut(s) 531, 670
Tru9I TTAA 2 cut(s) 531, 670
TscAI CASTG 2 cut(s) 309, 615
TseI GCWGC 3 cut(s) 96, 99, 403
TspDTI ATGAA 2 cut(s) 17, 210
TspRI CASTG 2 cut(s) 309, 615
Van91I CCANNNNNTGG 1 cut(s) 483
XapI RAATTY 2 cut(s) 130, 340
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 1 cut(s) 519
XspI CTAG 3 cut(s) 278, 365, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.