Rroxscaffold_3G00250900

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001702
Physical Location & Seq
Reverse (-)
44731817 .. 44733299
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250900.1

Sequence Viewer

Length: 744 bp
ATGAGTGATGATTTCATCGAACAATCTTGCAAGATAGCTGCTGGTCGTGAACCATCTTTGAACTACACCTTCTGTGTCTCAACTCTTAAGGCCAGCCCTAAAAGCCATGGTGCAGACCTTGATGGACTCATGTGCATCGTACTTCGCCTAGCCATAGACAAGGTGGACGCACCTGATATTTGTGAAACTGCGTTTAAAGAGAGGAAAACTCTCTCTTTTGAAGAAGGAGAACTATGCTCCAACAATTGGTCATCAAAATGTACCGATGAATCCCACGTCTTTAGCTACGACCTATGCACAACCTCTCTCCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTAGCACTAAACAATGCAAGCAACACGCTTCAGATCATAAAAAATTTGTTGATGAGCAACGAAAGTCTAGATCCTTCTGTTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATGCTGAAGGAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTATGGAAGCATCGAGTTCATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGAAGTATTACCGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCAATGTGCATAGGCCACTTGCTTAGCTCGGGTTCTACCTTCTTGATGAGGTCCTCTGCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

247

Amino Acids

26.79

Weight (kDa)

4.41

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 92 - 230 4.1e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 246
AclWI GGATC 2 cut(s) 434, 500
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 2 cut(s) 383, 534
AfaI GTAC 2 cut(s) 141, 262
AfiI CCNNNNNNNGG 1 cut(s) 246
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 4 cut(s) 61, 221, 463, 665
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 5 cut(s) 38, 285, 370, 478, 705
AluI AGCT 5 cut(s) 38, 285, 370, 478, 705
Alw26I GTCTC 2 cut(s) 82, 636
AlwI GGATC 2 cut(s) 434, 500
Ama87I CYCGRG 1 cut(s) 706
AoxI GGCC 2 cut(s) 90, 691
ApeKI GCWGC 2 cut(s) 38, 475
ApoI RAATTY 1 cut(s) 412
AspS9I GGNCC 1 cut(s) 729
AsuHPI GGTGA 2 cut(s) 542, 629
AsuNHI GCTAGC 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 706
AvaII GGWCC 1 cut(s) 729
BbvI GCAGC 2 cut(s) 25, 487
BccI CCATC 2 cut(s) 61, 116
BcoDI GTCTC 2 cut(s) 82, 636
BfaI CTAG 4 cut(s) 149, 350, 371, 437
BfrI CTTAAG 1 cut(s) 86
BisI GCNGC 2 cut(s) 39, 476
BlpI GCTNAGC 1 cut(s) 701
BlsI GCNGC 2 cut(s) 40, 477
Bme18I GGWCC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 706
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 729
BmsI GCATC 3 cut(s) 144, 499, 587
BmtI GCTAGC 1 cut(s) 374
BplI GAGNNNNNCTC 2 cut(s) 193, 225
BpmI CTGGAG 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 106, 343
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 507
Bse3DI GCAATG 2 cut(s) 369, 687
BseDI CCNNGG 2 cut(s) 106, 343
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMI GCAATG 2 cut(s) 369, 687
BseNI ACTGG 1 cut(s) 507
BseXI GCAGC 2 cut(s) 25, 487
BsgI GTGCAG 2 cut(s) 132, 585
BshFI GGCC 2 cut(s) 92, 693
BsiHKCI CYCGRG 1 cut(s) 706
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 246
BsmAI GTCTC 2 cut(s) 82, 636
BsmBI CGTCTC 1 cut(s) 636
BsnI GGCC 2 cut(s) 92, 693
BsoBI CYCGRG 1 cut(s) 706
Bsp143I GATC 3 cut(s) 402, 439, 492
Bsp1720I GCTNAGC 1 cut(s) 701
Bsp19I CCATGG 1 cut(s) 106
BspANI GGCC 2 cut(s) 92, 693
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 2 cut(s) 434, 500
BspTI CTTAAG 1 cut(s) 86
BsrDI GCAATG 2 cut(s) 369, 687
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 2 cut(s) 106, 343
BssMI GATC 3 cut(s) 402, 439, 492
BssT1I CCWWGG 2 cut(s) 106, 343
Bst4CI ACNGT 1 cut(s) 630
BstAFI CTTAAG 1 cut(s) 86
BstC8I GCNNGC 4 cut(s) 94, 372, 388, 473
BstDEI CTNAG 2 cut(s) 560, 701
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 3 cut(s) 405, 442, 495
BstMAI GTCTC 2 cut(s) 82, 636
BstMBI GATC 3 cut(s) 402, 439, 492
BstMWI GCNNNNNNNGC 3 cut(s) 102, 359, 518
BstV1I GCAGC 2 cut(s) 25, 487
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 2 cut(s) 92, 693
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 4 cut(s) 94, 372, 388, 473
Cfr13I GGNCC 1 cut(s) 729
CseI GACGC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 140, 261
CviAII CATG 4 cut(s) 107, 130, 329, 588
CviQI GTAC 2 cut(s) 140, 261
DdeI CTNAG 2 cut(s) 560, 701
DpnI GATC 3 cut(s) 404, 441, 494
DpnII GATC 3 cut(s) 402, 439, 492
DraI TTTAAA 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 106, 343
Eco47I GGWCC 1 cut(s) 729
Eco57I CTGAAG 2 cut(s) 383, 534
Eco88I CYCGRG 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 729
EcoT14I CCWWGG 2 cut(s) 106, 343
ErhI CCWWGG 2 cut(s) 106, 343
Esp3I CGTCTC 1 cut(s) 636
FaeI CATG 4 cut(s) 110, 133, 332, 591
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FatI CATG 4 cut(s) 106, 129, 328, 587
Fnu4HI GCNGC 2 cut(s) 39, 476
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 39, 476
FspBI CTAG 4 cut(s) 149, 350, 371, 437
GluI GCNGC 2 cut(s) 39, 476
GsuI CTGGAG 1 cut(s) 524
HaeIII GGCC 2 cut(s) 92, 693
HapII CCGG 1 cut(s) 320
HgaI GACGC 1 cut(s) 176
Hin1II CATG 4 cut(s) 110, 133, 332, 591
HinfI GANTC 2 cut(s) 126, 269
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 542, 629
Hpy166II GTNNAC 2 cut(s) 50, 166
Hpy188I TCNGA 2 cut(s) 402, 487
Hpy188III TCNNGA 3 cut(s) 47, 437, 721
Hpy8I GTNNAC 2 cut(s) 50, 166
HpyAV CCTTC 9 cut(s) 79, 218, 453, 457, 509, 590, 604, 608, 727
HpyCH4III ACNGT 1 cut(s) 630
HpyCH4IV ACGT 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 359, 518
HpyF3I CTNAG 2 cut(s) 560, 701
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 110, 133, 332, 591
Kzo9I GATC 3 cut(s) 402, 439, 492
LmnI GCTCC 3 cut(s) 242, 367, 518
LpnPI CCDG 6 cut(s) 27, 106, 186, 333, 488, 512
Lsp1109I GCAGC 2 cut(s) 25, 487
LweI GCATC 3 cut(s) 144, 499, 587
MaeI CTAG 4 cut(s) 149, 350, 371, 437
MaeII ACGT 1 cut(s) 276
MalI GATC 3 cut(s) 404, 441, 494
MboI GATC 3 cut(s) 402, 439, 492
MboII GAAGA 1 cut(s) 233
MfeI CAATTG 1 cut(s) 244
MflI RGATCY 1 cut(s) 439
MluCI AATT 4 cut(s) 244, 412, 649, 665
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 6 cut(s) 195, 313, 499, 586, 720, 742
MseI TTAA 3 cut(s) 87, 195, 603
MslI CAYNNNNRTG 1 cut(s) 256
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 244
MwoI GCNNNNNNNGC 3 cut(s) 102, 359, 518
NcoI CCATGG 1 cut(s) 106
NdeII GATC 3 cut(s) 402, 439, 492
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 4 cut(s) 110, 133, 332, 591
PfeI GAWTC 1 cut(s) 269
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 2 cut(s) 40, 477
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 729
Psp5II RGGWCCY 1 cut(s) 729
PspPI GGNCC 1 cut(s) 729
PspPPI RGGWCCY 1 cut(s) 729
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 2 cut(s) 141, 262
RsaNI GTAC 2 cut(s) 140, 261
RseI CAYNNNNRTG 1 cut(s) 256
SaqAI TTAA 3 cut(s) 87, 195, 603
SatI GCNGC 2 cut(s) 39, 476
Sau3AI GATC 3 cut(s) 402, 439, 492
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 1 cut(s) 120
SfaNI GCATC 3 cut(s) 144, 499, 587
SinI GGWCC 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 4 cut(s) 244, 412, 649, 665
SspI AATATT 1 cut(s) 553
SspMI CTAG 4 cut(s) 149, 350, 371, 437
StyI CCWWGG 2 cut(s) 106, 343
TaaI ACNGT 1 cut(s) 630
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 18, 581
TasI AATT 4 cut(s) 244, 412, 649, 665
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 87, 195, 603
Tru9I TTAA 3 cut(s) 87, 195, 603
TscAI CASTG 1 cut(s) 507
TseI GCWGC 2 cut(s) 38, 475
TspDTI ATGAA 3 cut(s) 4, 282, 576
TspRI CASTG 1 cut(s) 507
Van91I CCANNNNNTGG 1 cut(s) 246
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 729
XapI RAATTY 1 cut(s) 412
XbaI TCTAGA 1 cut(s) 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 4 cut(s) 149, 350, 371, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.