RLG00000028933

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001702
Physical Location & Seq
Forward (+)
30290048 .. 30290599
552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028933

Sequence Viewer

Length: 552 bp
ATGACATCTCCCATATTCATCTTCACATTCCTCCCTTTCTTCCTCCTCTTCTCATCAACCATAACTGCCAATAATCTCATCTCTGAAACCTGCAAGAACTGTGCTCAAAAAGACCCCAACCTGAGCTATAAGTTCTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCAAAACGCCGATCTCCGGCAACTCGGCCTCATCTCCATGAAGCTAGTCCAGTACAACGTGACCAACACAAGGCGCTTCATCAAGCAGCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGGTGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCTAGCATCAAGCAAGCCGTCAAGGATTACAAAATGAAGGACAACAGACACGCCAATATGGCAGTGAGTTCGGTCGCCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCCGGGTTGGTTTCGCCATTAACGAAGAGGAACCACGAGATGTTTCAGCTGTGTGTTATAGCGCTTATGATTATCAATATGGTCAGTTCGCCGTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

184

Amino Acids

20.67

Weight (kDa)

8.14

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 25 - 174 1.2e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 2 cut(s) 156, 417
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 1 cut(s) 221
AfeI AGCGCT 1 cut(s) 513
AfiI CCNNNNNNNGG 2 cut(s) 183, 237
AluBI AGCT 5 cut(s) 126, 211, 256, 274, 499
AluI AGCT 5 cut(s) 126, 211, 256, 274, 499
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 452
Aor51HI AGCGCT 1 cut(s) 513
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 253
AspLEI GCGC 2 cut(s) 243, 514
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 453, 454
AsuII TTCGAA 1 cut(s) 441
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 413
BauI CACGAG 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 265
BccI CCATC 1 cut(s) 428
BceAI ACGGC 2 cut(s) 335, 526
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BcnI CCSGG 2 cut(s) 453, 454
BcoDI GTCTC 1 cut(s) 442
BfaI CTAG 2 cut(s) 212, 336
BfoI RGCGCY 2 cut(s) 244, 515
BfuAI ACCTGC 1 cut(s) 98
BglI GCCNNNNNGGC 1 cut(s) 392
BisI GCNGC 3 cut(s) 156, 254, 417
BlsI GCNGC 3 cut(s) 157, 255, 418
Bme1390I CCNGG 2 cut(s) 453, 454
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 2 cut(s) 415, 482
BmrFI CCNGG 2 cut(s) 453, 454
BmsI GCATC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 453, 454
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 442
BsaJI CCNNGG 1 cut(s) 452
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 237
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 183, 237
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 2 cut(s) 35, 284
BseXI GCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 3 cut(s) 184, 411, 453
BslI CCNNNNNNNGG 2 cut(s) 183, 237
BsmAI GTCTC 1 cut(s) 442
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 2 cut(s) 156, 417
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 415, 482
BspMI ACCTGC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 410
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 1 cut(s) 178
BssNI GRCGYC 1 cut(s) 327
BssSI CACGAG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 485
Bst4CI ACNGT 2 cut(s) 101, 308
Bst6I CTCTTC 3 cut(s) 53, 438, 470
BstACI GRCGYC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 307
BstH2I RGCGCY 2 cut(s) 244, 515
BstHHI GCGC 2 cut(s) 243, 514
BstKTI GATC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 1 cut(s) 178
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 2 cut(s) 392, 416
BstSCI CCNGG 2 cut(s) 451, 452
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 2 cut(s) 243, 514
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 313
Cfr9I CCCGGG 1 cut(s) 452
CseI GACGC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 205
CviJI RGCY 9 cut(s) 126, 155, 168, 195, 211, 256, 274, 350, 499
CviKI_1 RGCY 9 cut(s) 126, 155, 168, 195, 211, 256, 274, 350, 499
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 1 cut(s) 122
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 53, 438, 470
EarI CTCTTC 3 cut(s) 53, 438, 470
Eco31I GGTCTC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 313
Eco47III AGCGCT 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 452
FaeI CATG 1 cut(s) 208
FaiI YATR 9 cut(s) 14, 62, 129, 206, 332, 392, 509, 518, 530
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 156, 254, 417
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 3 cut(s) 156, 254, 417
FspBI CTAG 2 cut(s) 212, 336
GlaI GCGC 2 cut(s) 242, 513
GluI GCNGC 3 cut(s) 156, 254, 417
HaeII RGCGCY 2 cut(s) 244, 515
HaeIII GGCC 1 cut(s) 195
HapII CCGG 3 cut(s) 184, 411, 453
HgaI GACGC 1 cut(s) 335
HhaI GCGC 2 cut(s) 243, 514
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 2 cut(s) 241, 512
HinP1I GCGC 2 cut(s) 241, 512
HinfI GANTC 1 cut(s) 438
HpaII CCGG 3 cut(s) 184, 411, 453
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 2 cut(s) 101, 308
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 416
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 2 cut(s) 241, 512
Kzo9I GATC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 6 cut(s) 103, 134, 197, 230, 424, 466
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 1 cut(s) 348
MaeI CTAG 2 cut(s) 212, 336
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 139, 226
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 6 cut(s) 13, 31, 40, 443, 455, 487
MhlI GDGCHC 1 cut(s) 106
MnlI CCTC 6 cut(s) 41, 53, 56, 206, 262, 471
MseI TTAA 2 cut(s) 470, 550
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 3 cut(s) 184, 411, 453
MspR9I CCNGG 2 cut(s) 453, 454
MwoI GCNNNNNNNGC 2 cut(s) 392, 416
NciI CCSGG 2 cut(s) 453, 454
NdeII GATC 1 cut(s) 178
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 2 cut(s) 415, 482
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 2 cut(s) 139, 226
NspV TTCGAA 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 470
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 3 cut(s) 157, 255, 418
PspN4I GGNNCC 2 cut(s) 415, 482
PspPI GGNCC 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 2 cut(s) 470, 550
SatI GCNGC 3 cut(s) 156, 254, 417
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 453, 454
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 1 cut(s) 348
SfuI TTCGAA 1 cut(s) 441
SgrAI CRCCGGYG 1 cut(s) 410
SinI GGWCC 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SsiI CCGC 2 cut(s) 156, 417
SspMI CTAG 2 cut(s) 212, 336
StyD4I CCNGG 2 cut(s) 451, 452
TaaI ACNGT 2 cut(s) 101, 308
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 1 cut(s) 394
TatI WGTACW 1 cut(s) 219
TauI GCSGC 2 cut(s) 158, 419
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 470, 550
Tru9I TTAA 2 cut(s) 470, 550
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 139, 226
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 2 cut(s) 139, 226
TspDTI ATGAA 4 cut(s) 7, 221, 235, 383
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 313
XmaI CCCGGG 1 cut(s) 452
XspI CTAG 2 cut(s) 212, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.