Rh1BG154100

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001702
Physical Location & Seq
Reverse (-)
25265186 .. 25266814
1629 bp
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UTR
Exon/CDS
Intron
Rh1BG154100.1

Sequence Viewer

Length: 666 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTCTCTTCCTCCTCATCTCATCAACCTTCATTCCCATAACTGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCTGAACGACCCCAACCTGAGCTACAAGTTCTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCACAGCGCCGATCTCCGGCGACTCGGCCTCATCTCCATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCACTTCATCAAGCATCTTCTCAAGAACAAGAAGCTTGAAAAGTTCGTACGGGCGCGTTTGGATGACTGTTTGGAGCTTTACACAGACGCCATACCCACCATCAAGCAAGCCATCAAGAATTACAAAAAGAAGCACAACAAAGACGCCAATATCGAAGTGAGTTCGATCGTCGATGCCTCCACGACTTGCGAAGATGGATTCGAGGAGAGACCAGGGTTGGTTTCGCCATTAACGAAAAGGAACAAAGAGACGTTTCAGCTGTGTATTATAGCGCTTTCGATTATCAATATGGTGACTCAAAAAGTGTTGTTTATCTTTATGGGTTCCCATCCATTCTATTTTTTGAAGCACAGGACGACCTCATCTCCGCTCTTGAGGAGGGCGACACCAACCCTCCCCGCCGGCCACCTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

221

Amino Acids

25.02

Weight (kDa)

9.65

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 177 1.3e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 1 cut(s) 304
AciI CCGC 3 cut(s) 165, 617, 648
AcoI YGGCCR 1 cut(s) 652
AcyI GRCGYC 2 cut(s) 336, 393
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 1 cut(s) 297
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AgsI TTSAA 2 cut(s) 287, 595
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 5 cut(s) 135, 220, 283, 325, 508
AluI AGCT 5 cut(s) 135, 220, 283, 325, 508
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 451, 491
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 2 cut(s) 202, 652
AspLEI GCGC 3 cut(s) 185, 304, 523
AsuHPI GGTGA 2 cut(s) 10, 553
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 4 cut(s) 356, 368, 437, 585
BciT130I CCWGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 451, 491
BfaI CTAG 1 cut(s) 221
BfoI RGCGCY 2 cut(s) 186, 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 271, 412
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 2 cut(s) 254, 643
BsaHI GRCGYC 2 cut(s) 336, 393
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 4 cut(s) 598, 627, 628, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
Bse118I RCCGGY 1 cut(s) 650
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 2 cut(s) 316, 577
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 3 cut(s) 35, 467, 640
Bsh1236I CGCG 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 2 cut(s) 204, 654
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 193, 651
BsiWI CGTACG 1 cut(s) 295
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 2 cut(s) 451, 491
BsmBI CGTCTC 1 cut(s) 491
BsnI GGCC 2 cut(s) 204, 654
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 187, 414
BspACI CCGC 3 cut(s) 165, 617, 648
BspANI GGCC 2 cut(s) 204, 654
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 619
BsrFI RCCGGY 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 2 cut(s) 187, 414
BssNI GRCGYC 2 cut(s) 336, 393
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 2 cut(s) 336, 393
BstC8I GCNNGC 2 cut(s) 357, 652
BstDEI CTNAG 1 cut(s) 131
BstF5I GGATG 2 cut(s) 316, 577
BstFNI CGCG 1 cut(s) 304
BstH2I RGCGCY 2 cut(s) 186, 524
BstHHI GCGC 3 cut(s) 185, 304, 523
BstKTI GATC 2 cut(s) 190, 417
BstMAI GTCTC 2 cut(s) 451, 491
BstMBI GATC 2 cut(s) 187, 414
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 1 cut(s) 304
BsuRI GGCC 2 cut(s) 204, 654
BtsCI GGATG 2 cut(s) 316, 577
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 2 cut(s) 357, 652
CfoI GCGC 3 cut(s) 185, 304, 523
Cfr10I RCCGGY 1 cut(s) 650
CseI GACGC 2 cut(s) 344, 401
Csp6I GTAC 1 cut(s) 296
CspCI CAANNNNNGTGG 1 cut(s) 644
CviAII CATG 1 cut(s) 214
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 1 cut(s) 131
DpnI GATC 2 cut(s) 189, 416
DpnII GATC 2 cut(s) 187, 414
DraIII CACNNNGTG 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 1 cut(s) 217
FaiI YATR 7 cut(s) 14, 71, 215, 341, 518, 539, 569
FatI CATG 1 cut(s) 213
FauI CCCGC 1 cut(s) 655
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 2 cut(s) 323, 564
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 1 cut(s) 221
GlaI GCGC 3 cut(s) 184, 303, 522
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 2 cut(s) 186, 524
HaeIII GGCC 2 cut(s) 204, 654
HapII CCGG 2 cut(s) 193, 651
HgaI GACGC 2 cut(s) 344, 401
HhaI GCGC 3 cut(s) 185, 304, 523
Hin1I GRCGYC 2 cut(s) 336, 393
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 3 cut(s) 183, 302, 521
HinP1I GCGC 3 cut(s) 183, 302, 521
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 3 cut(s) 198, 447, 544
HpaII CCGG 2 cut(s) 193, 651
HphI GGTGA 2 cut(s) 10, 553
Hpy188I TCNGA 2 cut(s) 94, 117
Hpy188III TCNNGA 3 cut(s) 271, 364, 622
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 31, 70
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 234, 500
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 234, 500
Hsp92I GRCGYC 2 cut(s) 336, 393
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 3 cut(s) 183, 302, 521
KroI GCCGGC 1 cut(s) 650
KroNI GCCGGC 1 cut(s) 652
Kzo9I GATC 2 cut(s) 187, 414
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 7 cut(s) 112, 143, 206, 239, 447, 474, 586
LweI GCATC 2 cut(s) 271, 412
MaeI CTAG 1 cut(s) 221
MaeII ACGT 2 cut(s) 234, 500
MaeIII GTNAC 3 cut(s) 148, 235, 541
MalI GATC 2 cut(s) 189, 416
MbiI CCGCTC 1 cut(s) 619
MboI GATC 2 cut(s) 187, 414
MboII GAAGA 3 cut(s) 31, 257, 452
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 367
MlyI GAGTC 2 cut(s) 192, 538
MroNI GCCGGC 1 cut(s) 650
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 2 cut(s) 193, 651
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 226
NaeI GCCGGC 1 cut(s) 652
NdeII GATC 2 cut(s) 187, 414
NgoMIV GCCGGC 1 cut(s) 650
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 1 cut(s) 574
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 3 cut(s) 148, 235, 541
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 479
PdiI GCCGGC 1 cut(s) 652
PfeI GAWTC 1 cut(s) 447
Pfl23II CGTACG 1 cut(s) 295
PkrI GCNGC 1 cut(s) 166
Ple19I CGATCG 1 cut(s) 417
PleI GAGTC 2 cut(s) 192, 538
PpsI GAGTC 2 cut(s) 192, 538
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspLI CGTACG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 574
PvuI CGATCG 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 2 cut(s) 187, 414
SchI GAGTC 2 cut(s) 192, 538
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 412
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 2 cut(s) 269, 622
SmoI CTYRAG 2 cut(s) 269, 622
Sse9I AATT 1 cut(s) 367
SsiI CCGC 3 cut(s) 165, 617, 648
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 237, 503
TaqI TCGA 5 cut(s) 402, 413, 420, 450, 527
TasI AATT 1 cut(s) 367
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TseFI GTSAC 3 cut(s) 148, 235, 541
Tsp45I GTSAC 3 cut(s) 148, 235, 541
TspDTI ATGAA 3 cut(s) 52, 230, 244
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.