Rh1BG153900

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001702
Physical Location & Seq
Reverse (-)
25258639 .. 25260422
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG153900.1

Sequence Viewer

Length: 468 bp
ATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCGCTTCATCAAGCATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGATGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCTAGCATCAAGCAAGCCGTCAAGGATTACAAAATGAAGGACAATAGACACGCCAATATCGAAGTGAGTTCGGTCACCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACAACGAGATGTTTCAGCTGTGTGTTATAGCGCTTAGGATTATCAATATGACTTCAATGCTAAACAACATTATAGCATTTCATAACACTAAGCTAAGCTACATGACGATTGATGAGGTACTGTCAATACAAAATGTTGAAGGAATTGTACAGATTTTCAAGCAGAAATGGGACATTGCCAAAGCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

155

Amino Acids

17.88

Weight (kDa)

7.72

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 106 2.6e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AciI CCGC 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 2 cut(s) 396, 426
AfeI AGCGCT 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 33
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 3 cut(s) 333, 416, 436
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 5 cut(s) 7, 70, 295, 370, 375
AluI AGCT 5 cut(s) 7, 70, 295, 370, 375
Alw26I GTCTC 1 cut(s) 238
Aor51HI AGCGCT 1 cut(s) 309
AsiGI ACCGGT 1 cut(s) 206
AspLEI GCGC 2 cut(s) 39, 310
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 237
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 209
BarI GAAGNNNNNNTAC 2 cut(s) 408, 440
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 2 cut(s) 8, 132
BfoI RGCGCY 2 cut(s) 40, 311
BisI GCNGC 1 cut(s) 213
BlpI GCTNAGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 58, 144
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 371
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 2 cut(s) 93, 103
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 209
BshTI ACCGGT 1 cut(s) 206
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 461
Bso31I GGTCTC 1 cut(s) 238
Bsp119I TTCGAA 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 371
BspACI CCGC 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 211
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 248
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 104, 399
Bst6I CTCTTC 2 cut(s) 234, 266
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 424
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 3 cut(s) 311, 366, 371
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 2 cut(s) 93, 103
BstH2I RGCGCY 2 cut(s) 40, 311
BstHHI GCGC 2 cut(s) 39, 310
BstMAI GTCTC 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNI CCWGG 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 93, 103
Cac8I GCNNGC 1 cut(s) 144
CfoI GCGC 2 cut(s) 39, 310
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 131
Csp6I GTAC 2 cut(s) 395, 425
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 1 cut(s) 379
CviJI RGCY 6 cut(s) 7, 70, 146, 295, 370, 375
CviKI_1 RGCY 6 cut(s) 7, 70, 146, 295, 370, 375
CviQI GTAC 2 cut(s) 395, 425
DdeI CTNAG 3 cut(s) 311, 366, 371
DraIII CACNNNGTG 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 234, 266
EarI CTCTTC 2 cut(s) 234, 266
Eco31I GGTCTC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 109
Eco47III AGCGCT 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 1 cut(s) 382
FaiI YATR 7 cut(s) 128, 305, 326, 350, 360, 380, 466
FaqI GGGAC 1 cut(s) 461
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 2 cut(s) 100, 110
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 2 cut(s) 8, 132
GlaI GCGC 2 cut(s) 38, 309
GluI GCNGC 1 cut(s) 213
HaeII RGCGCY 2 cut(s) 40, 311
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 131
HhaI GCGC 2 cut(s) 39, 310
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 2 cut(s) 37, 308
HinP1I GCGC 2 cut(s) 37, 308
HinfI GANTC 1 cut(s) 234
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 160, 410
HpyCH4III ACNGT 2 cut(s) 104, 399
HpyCH4IV ACGT 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 3 cut(s) 311, 366, 371
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 2 cut(s) 37, 308
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 4 cut(s) 26, 220, 234, 261
LweI GCATC 2 cut(s) 58, 144
MaeI CTAG 2 cut(s) 8, 132
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 22, 202
MboII GAAGA 4 cut(s) 44, 239, 251, 283
MluCI AATT 1 cut(s) 420
MnlI CCTC 3 cut(s) 58, 267, 385
MseI TTAA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 22, 202
NspV TTCGAA 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 266
PfeI GAWTC 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 2 cut(s) 396, 426
RsaNI GTAC 2 cut(s) 395, 425
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 249
SetI ASST 9 cut(s) 9, 24, 72, 114, 133, 297, 372, 377, 396
SfaNI GCATC 2 cut(s) 58, 144
SfuI TTCGAA 1 cut(s) 237
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 1 cut(s) 420
SsiI CCGC 1 cut(s) 213
SspMI CTAG 2 cut(s) 8, 132
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 104, 399
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 189, 237
TaqII GACCGA 1 cut(s) 190
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseFI GTSAC 2 cut(s) 22, 202
Tsp45I GTSAC 2 cut(s) 22, 202
TspDTI ATGAA 4 cut(s) 17, 31, 179, 347
VpaK11BI GGWCC 1 cut(s) 109
XspI CTAG 2 cut(s) 8, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.