Rw0G014110

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00633
OG0001702
Physical Location & Seq
Forward (+)
27032 .. 27873
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014110.1

Sequence Viewer

Length: 612 bp
ATGCACCATATTTCTTTCTTTTCAATCTCTTTATGCTGCTGCTTATTTTTCCTCACAATTCCTCACGAAAATTTCTCACCAATTACTGGAATATCTGTTGATATAATCAACCAAACCTGCAGAAAATGTGCCGATGAATCCCATGTCTTTAGCTACGACCTATGCACAACCTCTCTTCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTAGCACTAAACAATGCAAGCAGCACGCTTCAGATCATAAAAAATTTGTTGATGAGCAACGAAAGTCTAGATCCTTTTGTTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATGCTGAAGAAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTATGGAAGCATCGAGTACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGAAGTATTACCGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCACTGTGCATAGGCCACTTGCTTACCTCGGGTTCTACCTTGTTGATGAGGCCCTCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

22.19

Weight (kDa)

4.45

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 186 1.2e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 86
AclWI GGATC 2 cut(s) 302, 368
AcsI RAATTY 2 cut(s) 70, 280
AcuI CTGAAG 2 cut(s) 251, 402
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 4 cut(s) 24, 179, 331, 533
AluBI AGCT 3 cut(s) 153, 238, 346
AluI AGCT 3 cut(s) 153, 238, 346
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 302, 368
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 2 cut(s) 559, 596
ApeKI GCWGC 4 cut(s) 36, 39, 258, 343
ApoI RAATTY 2 cut(s) 70, 280
Asp700I GAANNNNTTC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 3 cut(s) 69, 410, 497
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 574
BbvI GCAGC 4 cut(s) 23, 26, 270, 355
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 3 cut(s) 218, 239, 305
BfmI CTRYAG 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 125
BisI GCNGC 4 cut(s) 37, 40, 259, 344
BlsI GCNGC 4 cut(s) 38, 41, 260, 345
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 597
BmsI GCATC 2 cut(s) 367, 455
BmtI GCTAGC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 211, 573
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 91, 375
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 2 cut(s) 211, 573
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 1 cut(s) 237
BseNI ACTGG 2 cut(s) 91, 375
BseXI GCAGC 4 cut(s) 23, 26, 270, 355
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 561, 598
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 504
BsmBI CGTCTC 1 cut(s) 504
BsnI GGCC 2 cut(s) 561, 598
BsoBI CYCGRG 1 cut(s) 574
Bsp143I GATC 3 cut(s) 270, 307, 360
BspANI GGCC 2 cut(s) 561, 598
BspMAI CTGCAG 1 cut(s) 122
BspMI ACCTGC 1 cut(s) 125
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 2 cut(s) 302, 368
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 2 cut(s) 91, 375
BssECI CCNNGG 2 cut(s) 211, 573
BssMI GATC 3 cut(s) 270, 307, 360
BssT1I CCWWGG 1 cut(s) 211
Bst4CI ACNGT 2 cut(s) 498, 552
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 4 cut(s) 240, 256, 263, 341
BstDEI CTNAG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 3 cut(s) 273, 310, 363
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 3 cut(s) 270, 307, 360
BstMWI GCNNNNNNNGC 2 cut(s) 227, 386
BstNSI RCATGY 1 cut(s) 459
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 4 cut(s) 23, 26, 270, 355
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 2 cut(s) 561, 598
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 368, 548
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 4 cut(s) 240, 256, 263, 341
Cfr13I GGNCC 1 cut(s) 597
Csp6I GTAC 1 cut(s) 453
CviAII CATG 3 cut(s) 143, 197, 456
CviJI RGCY 8 cut(s) 153, 195, 203, 238, 346, 468, 561, 598
CviKI_1 RGCY 8 cut(s) 153, 195, 203, 238, 346, 468, 561, 598
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 1 cut(s) 428
DpnI GATC 3 cut(s) 272, 309, 362
DpnII GATC 3 cut(s) 270, 307, 360
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 251, 402
Eco88I CYCGRG 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 597
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 504
FaeI CATG 3 cut(s) 146, 200, 459
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 3 cut(s) 142, 196, 455
Fnu4HI GCNGC 4 cut(s) 37, 40, 259, 344
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 4 cut(s) 37, 40, 259, 344
FspBI CTAG 3 cut(s) 218, 239, 305
GluI GCNGC 4 cut(s) 37, 40, 259, 344
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 2 cut(s) 561, 598
HapII CCGG 1 cut(s) 188
Hin1II CATG 3 cut(s) 146, 200, 459
HinfI GANTC 1 cut(s) 137
HpaII CCGG 1 cut(s) 188
HphI GGTGA 3 cut(s) 69, 410, 497
Hpy188I TCNGA 2 cut(s) 270, 355
Hpy188III TCNNGA 2 cut(s) 65, 305
HpyAV CCTTC 4 cut(s) 325, 458, 472, 476
HpyCH4III ACNGT 2 cut(s) 498, 552
HpyCH4V TGCA 9 cut(s) 4, 120, 165, 254, 343, 434, 548, 555, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 386
HpyF3I CTNAG 1 cut(s) 428
Hsp92II CATG 3 cut(s) 146, 200, 459
Kzo9I GATC 3 cut(s) 270, 307, 360
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 5 cut(s) 72, 130, 201, 356, 380
Lsp1109I GCAGC 4 cut(s) 23, 26, 270, 355
LweI GCATC 2 cut(s) 367, 455
MaeI CTAG 3 cut(s) 218, 239, 305
MalI GATC 3 cut(s) 272, 309, 362
MboI GATC 3 cut(s) 270, 307, 360
MboII GAAGA 2 cut(s) 167, 395
MflI RGATCY 1 cut(s) 307
MluCI AATT 6 cut(s) 57, 70, 81, 280, 517, 533
MnlI CCTC 8 cut(s) 62, 72, 181, 367, 454, 583, 588, 610
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 2 cut(s) 471, 610
MspI CCGG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 227, 386
NdeII GATC 3 cut(s) 270, 307, 360
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 146, 200, 459
NspI RCATGY 1 cut(s) 459
PciI ACATGT 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 86
PkrI GCNGC 4 cut(s) 38, 41, 260, 345
PscI ACATGT 1 cut(s) 455
PspPI GGNCC 1 cut(s) 597
PstI CTGCAG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 471, 610
SatI GCNGC 4 cut(s) 37, 40, 259, 344
Sau3AI GATC 3 cut(s) 270, 307, 360
Sau96I GGNCC 1 cut(s) 597
SetI ASST 9 cut(s) 119, 155, 162, 173, 240, 348, 487, 575, 587
SfaNI GCATC 2 cut(s) 367, 455
SfcI CTRYAG 1 cut(s) 118
Sse9I AATT 6 cut(s) 57, 70, 81, 280, 517, 533
SspI AATATT 1 cut(s) 421
SspMI CTAG 3 cut(s) 218, 239, 305
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 2 cut(s) 498, 552
TaqI TCGA 1 cut(s) 449
TasI AATT 6 cut(s) 57, 70, 81, 280, 517, 533
TatI WGTACW 1 cut(s) 452
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 2 cut(s) 471, 610
Tru9I TTAA 2 cut(s) 471, 610
TscAI CASTG 2 cut(s) 375, 555
TseI GCWGC 4 cut(s) 36, 39, 258, 343
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 2 cut(s) 375, 555
Van91I CCANNNNNTGG 1 cut(s) 86
XapI RAATTY 2 cut(s) 70, 280
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 3 cut(s) 218, 239, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.