RLG00000022936

inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001702
Physical Location & Seq
Reverse (-)
15445688 .. 15446786
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022936

Sequence Viewer

Length: 732 bp
ATGATAGATGATGAAAAACTTATGGATGCTCTTATAATTTCTGATTGCTTTCTAAGCAATGGAGTTTCAGATCAACTATTTCAGCTACTCGTTGAACATGGGGAAGAAAACCAGTTAGTCTCTGGACACTCTCAGGGTTTTAGTGGCAATAGCATTTGGAGCACTAGTTGGCAGTATTGCCTGCGGTTGAAGGATAAGCAAGTGGCGAGTGCTTTGTGTAGAGTTACCCTGAAACTAAAGATGATGCTCACAGTCCTTCTTGCTCTCCTTGTTTTGCAACAATCCTCAACATTTGTGAGTGCTGATTTGATAGACGAGGCGTGTAACAAGACCTCTAATTCCACGCTCTGTGAGTTAGTTTTAAGATCAACACCTGGTAGTGCTGCTGCAGATGCCAAAGGGCTTGCTCTGATCGTGATTGAACAGGTACAGATCGATGTTCAACAAATATATTCCCTTTCAATCAAACTAATGAGGCAGGCAACCGATGGAATTCTAAAGCAATGTTTTCATATTTGCTCTGATGTATATGGTATGGATGTCGATCGTATGGTTTGTACCATTGCTAATTTGAATACAAATAATTTTGACGCGGTATTGGATGGCCTTGGGGCTACATGGAGCGATGCAGACACTTGTGAAGCTTCATTCCATGAGGATCCGCCTCGACCATCGCCATTAACAGACCAGAACAATTATATTAAGAATTTAACTCTAGTTGCGGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

244

Amino Acids

26.61

Weight (kDa)

4.53

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 102 - 239 5.8e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AccII CGCG 1 cut(s) 593
AciI CCGC 4 cut(s) 184, 593, 662, 722
AclWI GGATC 2 cut(s) 653, 666
AcsI RAATTY 2 cut(s) 492, 706
AfaI GTAC 2 cut(s) 429, 559
AgsI TTSAA 6 cut(s) 95, 190, 422, 443, 462, 574
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 2 cut(s) 85, 644
AluI AGCT 2 cut(s) 85, 644
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 2 cut(s) 653, 666
AoxI GGCC 1 cut(s) 604
ApeKI GCWGC 2 cut(s) 383, 386
ApoI RAATTY 2 cut(s) 492, 706
BamHI GGATCC 1 cut(s) 658
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 2 cut(s) 370, 373
BccI CCATC 3 cut(s) 482, 596, 679
BciT130I CCWGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 124
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 2 cut(s) 165, 716
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 3 cut(s) 384, 387, 723
BlsI GCNGC 3 cut(s) 385, 388, 724
Bme1390I CCNGG 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 660
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 4 cut(s) 16, 234, 382, 616
Bsa29I ATCGAT 1 cut(s) 435
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 1 cut(s) 607
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 3 cut(s) 64, 509, 561
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 375
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 3 cut(s) 31, 544, 607
BseJI GATNNNNATC 1 cut(s) 543
BseMI GCAATG 3 cut(s) 64, 509, 561
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 370, 373
Bsh1236I CGCG 1 cut(s) 593
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 1 cut(s) 606
BshVI ATCGAT 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 124
BsnI GGCC 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 6 cut(s) 70, 365, 411, 432, 544, 658
BspACI CCGC 4 cut(s) 184, 593, 662, 722
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 435
BspFNI CGCG 1 cut(s) 593
BspLI GGNNCC 1 cut(s) 660
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 2 cut(s) 653, 666
BsrDI GCAATG 3 cut(s) 64, 509, 561
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 6 cut(s) 70, 365, 411, 432, 544, 658
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 182, 405, 480
BstDEI CTNAG 2 cut(s) 53, 132
BstF5I GGATG 3 cut(s) 31, 544, 607
BstFNI CGCG 1 cut(s) 593
BstKTI GATC 6 cut(s) 73, 368, 414, 435, 547, 661
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 6 cut(s) 70, 365, 411, 432, 544, 658
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 3 cut(s) 54, 159, 392
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 593
BstV1I GCAGC 2 cut(s) 370, 373
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 435
BsuRI GGCC 1 cut(s) 606
BsuTUI ATCGAT 1 cut(s) 435
BtgZI GCGATG 2 cut(s) 639, 657
BtsCI GGATG 3 cut(s) 31, 544, 607
Cac8I GCNNGC 3 cut(s) 182, 405, 480
ClaI ATCGAT 1 cut(s) 435
CseI GACGC 1 cut(s) 599
CsiI ACCWGGT 1 cut(s) 373
Csp6I GTAC 2 cut(s) 428, 558
CviAII CATG 3 cut(s) 98, 618, 653
CviJI RGCY 6 cut(s) 85, 403, 606, 614, 644, 725
CviKI_1 RGCY 6 cut(s) 85, 403, 606, 614, 644, 725
CviQI GTAC 2 cut(s) 428, 558
DdeI CTNAG 2 cut(s) 53, 132
DpnI GATC 6 cut(s) 72, 367, 413, 434, 546, 660
DpnII GATC 6 cut(s) 70, 365, 411, 432, 544, 658
EciI GGCGGA 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 3 cut(s) 101, 621, 656
FatI CATG 3 cut(s) 97, 617, 652
Fnu4HI GCNGC 3 cut(s) 384, 387, 723
FokI GGATG 3 cut(s) 38, 551, 614
Fsp4HI GCNGC 3 cut(s) 384, 387, 723
FspBI CTAG 2 cut(s) 165, 716
GluI GCNGC 3 cut(s) 384, 387, 723
HaeIII GGCC 1 cut(s) 606
HgaI GACGC 1 cut(s) 599
Hin1II CATG 3 cut(s) 101, 621, 656
HindIII AAGCTT 1 cut(s) 642
Hpy188I TCNGA 4 cut(s) 43, 70, 411, 523
Hpy188III TCNNGA 2 cut(s) 123, 415
HpyAV CCTTC 2 cut(s) 184, 266
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 277, 389, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 159, 392
HpyF3I CTNAG 2 cut(s) 53, 132
Hsp92II CATG 3 cut(s) 101, 621, 656
Kzo9I GATC 6 cut(s) 70, 365, 411, 432, 544, 658
LmnI GCTCC 2 cut(s) 159, 621
Lsp1109I GCAGC 2 cut(s) 370, 373
LweI GCATC 4 cut(s) 16, 234, 382, 616
MabI ACCWGGT 1 cut(s) 373
MaeI CTAG 2 cut(s) 165, 716
MaeIII GTNAC 2 cut(s) 223, 323
MalI GATC 6 cut(s) 72, 367, 413, 434, 546, 660
MboI GATC 6 cut(s) 70, 365, 411, 432, 544, 658
MboII GAAGA 1 cut(s) 116
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 7 cut(s) 36, 337, 492, 568, 583, 694, 706
MnlI CCTC 6 cut(s) 295, 310, 343, 468, 649, 675
MseI TTAA 4 cut(s) 362, 680, 702, 710
MspR9I CCNGG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 375
MvnI CGCG 1 cut(s) 593
MwoI GCNNNNNNNGC 3 cut(s) 54, 159, 392
NdeII GATC 6 cut(s) 70, 365, 411, 432, 544, 658
NlaIII CATG 3 cut(s) 101, 621, 656
NlaIV GGNNCC 1 cut(s) 660
PkrI GCNGC 3 cut(s) 385, 388, 724
Ple19I CGATCG 1 cut(s) 547
PsiI TTATAA 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 660
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 658
PvuI CGATCG 1 cut(s) 547
RsaI GTAC 2 cut(s) 429, 559
RsaNI GTAC 2 cut(s) 428, 558
SaqAI TTAA 4 cut(s) 362, 680, 702, 710
SatI GCNGC 3 cut(s) 384, 387, 723
Sau3AI GATC 6 cut(s) 70, 365, 411, 432, 544, 658
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 1 cut(s) 164
SetI ASST 5 cut(s) 87, 335, 376, 429, 646
SexAI ACCWGGT 1 cut(s) 373
SfaNI GCATC 4 cut(s) 16, 234, 382, 616
SfcI CTRYAG 1 cut(s) 387
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 7 cut(s) 36, 337, 492, 568, 583, 694, 706
SsiI CCGC 4 cut(s) 184, 593, 662, 722
SspMI CTAG 2 cut(s) 165, 716
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 1 cut(s) 253
TaqI TCGA 3 cut(s) 435, 543, 667
TasI AATT 7 cut(s) 36, 337, 492, 568, 583, 694, 706
TauI GCSGC 1 cut(s) 725
Tru1I TTAA 4 cut(s) 362, 680, 702, 710
Tru9I TTAA 4 cut(s) 362, 680, 702, 710
TseI GCWGC 2 cut(s) 383, 386
TspDTI ATGAA 3 cut(s) 27, 500, 636
XapI RAATTY 2 cut(s) 492, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XspI CTAG 2 cut(s) 165, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.