Rh7CG249900

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001702
Physical Location & Seq
Forward (+)
23580445 .. 23581116
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG249900.1

Sequence Viewer

Length: 672 bp
ATGAATTTCATATTAGAACTACAAATATTCCACTACATGAGCAAGAAACCAGAGGAAACAATGCACCATATTTCTTTCTTTCCAATCTCTTTATGCTGCTGCTTATTTTTCCTCATAATTTCTCACGAAAATTTCTCACCAATTACTGCAATATCTGTTGATATAATCAACCAAACCTGCAGAAAATGTGCTGATGAATCCCACGTCTTTAGCTACGACCTATGCACAACCTCTCTCCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTAGCACTAAACAATGCAAGCAACACGCTTCAGATCATAAAAAATTTGTTGATGAGCAACGAAAGTCTAGATCCTTTTGTTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATACTGAAGGAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTATGGAAGCATCGAGTACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGAAGTATTACCGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCACTGTGCATAGGCCACTTGCTTACCTCGGGTTCTACCTTCTTGATGAGGTCCTCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

223

Amino Acids

24.71

Weight (kDa)

4.58

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 55 - 206 2.1e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AclWI GGATC 2 cut(s) 362, 428
AcsI RAATTY 3 cut(s) 4, 130, 340
AcuI CTGAAG 2 cut(s) 311, 462
AfaI GTAC 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 2 cut(s) 391, 593
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 3 cut(s) 213, 298, 406
AluI AGCT 3 cut(s) 213, 298, 406
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 2 cut(s) 362, 428
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 1 cut(s) 619
ApeKI GCWGC 3 cut(s) 96, 99, 403
ApoI RAATTY 3 cut(s) 4, 130, 340
AspS9I GGNCC 1 cut(s) 657
AsuHPI GGTGA 3 cut(s) 129, 470, 557
AsuNHI GCTAGC 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 1 cut(s) 657
BbvI GCAGC 3 cut(s) 83, 86, 415
BciVI GTATCC 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 3 cut(s) 278, 299, 365
BfmI CTRYAG 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 185
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 3 cut(s) 97, 100, 404
BlsI GCNGC 3 cut(s) 98, 101, 405
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 634
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 657
BmsI GCATC 1 cut(s) 515
BmtI GCTAGC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 271, 633
BsaWI WCCGGW 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 271, 633
BseMI GCAATG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 435
BseXI GCAGC 3 cut(s) 83, 86, 415
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 564
BsmBI CGTCTC 1 cut(s) 564
BsnI GGCC 1 cut(s) 621
BsoBI CYCGRG 1 cut(s) 634
Bsp143I GATC 3 cut(s) 330, 367, 420
BspANI GGCC 1 cut(s) 621
BspMAI CTGCAG 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 302
BspPI GGATC 2 cut(s) 362, 428
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 271, 633
BssMI GATC 3 cut(s) 330, 367, 420
BssT1I CCWWGG 1 cut(s) 271
Bst4CI ACNGT 2 cut(s) 558, 612
BstC8I GCNNGC 3 cut(s) 300, 316, 401
BstDEI CTNAG 1 cut(s) 488
BstKTI GATC 3 cut(s) 333, 370, 423
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 3 cut(s) 330, 367, 420
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 83, 86, 415
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 1 cut(s) 621
BtrI CACGTC 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 428, 608
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 300, 316, 401
Cfr13I GGNCC 1 cut(s) 657
Csp6I GTAC 1 cut(s) 513
CviAII CATG 3 cut(s) 37, 257, 516
CviJI RGCY 7 cut(s) 213, 255, 263, 298, 406, 528, 621
CviKI_1 RGCY 7 cut(s) 213, 255, 263, 298, 406, 528, 621
CviQI GTAC 1 cut(s) 513
DdeI CTNAG 1 cut(s) 488
DpnI GATC 3 cut(s) 332, 369, 422
DpnII GATC 3 cut(s) 330, 367, 420
Eco130I CCWWGG 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 657
Eco57I CTGAAG 2 cut(s) 311, 462
Eco88I CYCGRG 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 3 cut(s) 40, 260, 519
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 3 cut(s) 36, 256, 515
Fnu4HI GCNGC 3 cut(s) 97, 100, 404
Fsp4HI GCNGC 3 cut(s) 97, 100, 404
FspBI CTAG 3 cut(s) 278, 299, 365
GluI GCNGC 3 cut(s) 97, 100, 404
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 621
HapII CCGG 1 cut(s) 248
Hin1II CATG 3 cut(s) 40, 260, 519
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 129, 470, 557
Hpy188I TCNGA 2 cut(s) 330, 415
Hpy188III TCNNGA 3 cut(s) 125, 365, 649
HpyAV CCTTC 6 cut(s) 385, 437, 518, 532, 536, 655
HpyCH4III ACNGT 2 cut(s) 558, 612
HpyCH4IV ACGT 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 1 cut(s) 488
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 3 cut(s) 40, 260, 519
Kzo9I GATC 3 cut(s) 330, 367, 420
LmnI GCTCC 2 cut(s) 295, 446
LpnPI CCDG 5 cut(s) 63, 190, 261, 416, 440
Lsp1109I GCAGC 3 cut(s) 83, 86, 415
LweI GCATC 1 cut(s) 515
MaeI CTAG 3 cut(s) 278, 299, 365
MaeII ACGT 1 cut(s) 204
MalI GATC 3 cut(s) 332, 369, 422
MboI GATC 3 cut(s) 330, 367, 420
MflI RGATCY 1 cut(s) 367
MluCI AATT 7 cut(s) 4, 117, 130, 141, 340, 577, 593
MnlI CCTC 8 cut(s) 46, 122, 241, 427, 514, 643, 648, 670
MseI TTAA 2 cut(s) 531, 670
MspI CCGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 287
NdeII GATC 3 cut(s) 330, 367, 420
NheI GCTAGC 1 cut(s) 298
NlaIII CATG 3 cut(s) 40, 260, 519
NspI RCATGY 1 cut(s) 519
PciI ACATGT 1 cut(s) 515
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 3 cut(s) 98, 101, 405
PpuMI RGGWCCY 1 cut(s) 657
PscI ACATGT 1 cut(s) 515
Psp5II RGGWCCY 1 cut(s) 657
PspPI GGNCC 1 cut(s) 657
PspPPI RGGWCCY 1 cut(s) 657
PstI CTGCAG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 1 cut(s) 514
RsaNI GTAC 1 cut(s) 513
SaqAI TTAA 2 cut(s) 531, 670
SatI GCNGC 3 cut(s) 97, 100, 404
Sau3AI GATC 3 cut(s) 330, 367, 420
Sau96I GGNCC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 657
Sse9I AATT 7 cut(s) 4, 117, 130, 141, 340, 577, 593
SspI AATATT 2 cut(s) 27, 481
SspMI CTAG 3 cut(s) 278, 299, 365
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 558, 612
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 509
TasI AATT 7 cut(s) 4, 117, 130, 141, 340, 577, 593
TatI WGTACW 1 cut(s) 512
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 531, 670
Tru9I TTAA 2 cut(s) 531, 670
TscAI CASTG 2 cut(s) 435, 615
TseI GCWGC 3 cut(s) 96, 99, 403
TspDTI ATGAA 2 cut(s) 17, 210
TspRI CASTG 2 cut(s) 435, 615
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 3 cut(s) 4, 130, 340
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 1 cut(s) 519
XspI CTAG 3 cut(s) 278, 299, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.