Rh1AG187800

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001702
Physical Location & Seq
Reverse (-)
35786794 .. 35789865
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG187800.1

Sequence Viewer

Length: 879 bp
ATGGGAGTTGCCATATTCACCTTCACATTCCTCCCTTTCTTCCTCCTCTTCTCATCAACCTTGACTCCCATAACAGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCAAAAAGACCCCAACCTGAGCTACAAGTTTTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCAAGACGCCGATCTCCGGCAACTCGGCCTCATCTCCATGAATCTAGTCCAGAACAACGTGACCAACACAAGGCGCTTCATCAAGAATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGGTGTATTTGGATGACTGTTTGGACCTGTACACAGACGCCATACCTAGCATCAAGCAAGCTGTCAAGGATTACAAAATGAAGGACAACAGACACGCCAATATCGCAGTGAGTTCGGTCGCCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACCACGAGATGTTTCAGCTCTCATGTAATGAGACAAGCCTGCTGCTAAAGGTAGTTACGAAGCCATTGGATGGTTATAGATGTACTCAATGGCCAGATTTGCAGATTCAATCTTATCAGCTTGAGGCCATGTCTGTGGCGGCGGCGGCGGCGTCTTCATCAGAGGAGGAAGAGGTGGAGGACACTGATCAAACTCAAACCACCATCTCGCCGTCCTCTGCGAAGAAGAGGCAAAATCTCCGATTTCATGCGGAGTTGAACCAAGTCCGTCTATTCCAGAGGGCCTTGAGACCCCTGCTTCAGACTGCCTACACCGACCGAGAGTACCCGGACGATATGTTCCTCATCGGACACATCCTTGATGCCGGCCACTTCTGCATATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

292

Amino Acids

32.88

Weight (kDa)

5.61

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 173 9.7e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 560
AciI CCGC 7 cut(s) 165, 426, 629, 632, 635, 638, 740
AcoI YGGCCR 2 cut(s) 581, 856
AcuI CTGAAG 1 cut(s) 773
AcyI GRCGYC 3 cut(s) 183, 336, 641
AfaI GTAC 3 cut(s) 329, 574, 815
AfiI CCNNNNNNNGG 3 cut(s) 192, 246, 560
AgsI TTSAA 2 cut(s) 599, 748
AjnI CCWGG 1 cut(s) 460
AloI GAACNNNNNNTCC 2 cut(s) 812, 844
AluBI AGCT 5 cut(s) 135, 283, 359, 508, 610
AluI AGCT 5 cut(s) 135, 283, 359, 508, 610
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 3 cut(s) 451, 515, 772
AoxI GGCC 5 cut(s) 202, 581, 615, 771, 856
ApeKI GCWGC 1 cut(s) 532
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 322, 771
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 2 cut(s) 10, 864
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 583
BanI GGYRCC 1 cut(s) 422
BauI CACGAG 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 519
BccI CCATC 3 cut(s) 437, 554, 701
BceAI ACGGC 1 cut(s) 685
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 3 cut(s) 451, 515, 772
BfaI CTAG 2 cut(s) 221, 345
BfoI RGCGCY 1 cut(s) 253
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 7 cut(s) 165, 426, 533, 630, 633, 636, 639
BlsI GCNGC 7 cut(s) 166, 427, 534, 631, 634, 637, 640
Bme1390I CCNGG 2 cut(s) 462, 818
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 322, 771
BmiI GGNNCC 2 cut(s) 424, 491
BmrFI CCNGG 2 cut(s) 462, 818
BmsI GCATC 2 cut(s) 357, 841
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 3 cut(s) 254, 632, 796
BpuMI CCSGG 1 cut(s) 818
BsaHI GRCGYC 3 cut(s) 183, 336, 641
BsaI GGTCTC 2 cut(s) 451, 772
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 246, 560
Bse118I RCCGGY 2 cut(s) 419, 854
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 3 cut(s) 316, 565, 843
BseLI CCNNNNNNNGG 3 cut(s) 192, 246, 560
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 3 cut(s) 35, 293, 668
BseXI GCAGC 1 cut(s) 519
Bsh1285I CGRYCG 2 cut(s) 417, 808
BshFI GGCC 5 cut(s) 204, 583, 617, 773, 858
BshNI GGYRCC 1 cut(s) 422
BsiEI CGRYCG 2 cut(s) 417, 808
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 4 cut(s) 193, 420, 818, 855
BslI CCNNNNNNNGG 3 cut(s) 192, 246, 560
BsmAI GTCTC 3 cut(s) 451, 515, 772
BsnI GGCC 5 cut(s) 204, 583, 617, 773, 858
Bso31I GGTCTC 2 cut(s) 451, 772
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 115
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 2 cut(s) 187, 676
BspACI CCGC 7 cut(s) 165, 426, 629, 632, 635, 638, 740
BspANI GGCC 5 cut(s) 204, 583, 617, 773, 858
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 424, 491
BspMI ACCTGC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 2 cut(s) 451, 772
BsrFI RCCGGY 2 cut(s) 419, 854
BsrGI TGTACA 1 cut(s) 327
BssAI RCCGGY 2 cut(s) 419, 854
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 2 cut(s) 187, 676
BssNI GRCGYC 3 cut(s) 183, 336, 641
BssSI CACGAG 1 cut(s) 494
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 5 cut(s) 53, 447, 479, 654, 710
BstACI GRCGYC 3 cut(s) 183, 336, 641
BstAUI TGTACA 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 3 cut(s) 357, 530, 856
BstDEI CTNAG 1 cut(s) 131
BstF5I GGATG 3 cut(s) 316, 565, 843
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 2 cut(s) 190, 679
BstMAI GTCTC 3 cut(s) 451, 515, 772
BstMBI GATC 2 cut(s) 187, 676
BstMCI CGRYCG 2 cut(s) 417, 808
BstMWI GCNNNNNNNGC 6 cut(s) 401, 425, 589, 635, 638, 864
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 2 cut(s) 460, 816
BstV1I GCAGC 1 cut(s) 519
BstV2I GAAGAC 1 cut(s) 636
BstXI CCANNNNNNTGG 1 cut(s) 625
BsuRI GGCC 5 cut(s) 204, 583, 617, 773, 858
BtsCI GGATG 3 cut(s) 316, 565, 843
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 411, 672
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 3 cut(s) 357, 530, 856
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 2 cut(s) 419, 854
Cfr13I GGNCC 2 cut(s) 322, 771
CseI GACGC 3 cut(s) 191, 344, 630
Csp6I GTAC 3 cut(s) 328, 573, 814
CviAII CATG 5 cut(s) 214, 513, 619, 737, 876
CviQI GTAC 3 cut(s) 328, 573, 814
DdeI CTNAG 1 cut(s) 131
DpnI GATC 2 cut(s) 189, 678
DpnII GATC 2 cut(s) 187, 676
EaeI YGGCCR 2 cut(s) 581, 856
Eam1104I CTCTTC 5 cut(s) 53, 447, 479, 654, 710
EarI CTCTTC 5 cut(s) 53, 447, 479, 654, 710
Eco31I GGTCTC 2 cut(s) 451, 772
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 771
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 5 cut(s) 217, 516, 622, 740, 879
FatI CATG 5 cut(s) 213, 512, 618, 736, 875
FbaI TGATCA 1 cut(s) 676
Fnu4HI GCNGC 7 cut(s) 165, 426, 533, 630, 633, 636, 639
FokI GGATG 3 cut(s) 323, 572, 830
Fsp4HI GCNGC 7 cut(s) 165, 426, 533, 630, 633, 636, 639
FspBI CTAG 2 cut(s) 221, 345
GlaI GCGC 1 cut(s) 251
GluI GCNGC 7 cut(s) 165, 426, 533, 630, 633, 636, 639
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 5 cut(s) 204, 583, 617, 773, 858
HapII CCGG 4 cut(s) 193, 420, 818, 855
HgaI GACGC 3 cut(s) 191, 344, 630
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 3 cut(s) 183, 336, 641
Hin1II CATG 5 cut(s) 217, 516, 622, 740, 879
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 5 cut(s) 64, 217, 262, 447, 595
HpaII CCGG 4 cut(s) 193, 420, 818, 855
HphI GGTGA 2 cut(s) 10, 864
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 5 cut(s) 94, 652, 731, 792, 839
Hpy188III TCNNGA 4 cut(s) 226, 259, 271, 766
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 2 cut(s) 31, 373
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 102, 592, 867
HpyF10VI GCNNNNNNNGC 6 cut(s) 401, 425, 589, 635, 638, 864
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 3 cut(s) 183, 336, 641
Hsp92II CATG 5 cut(s) 217, 516, 622, 740, 879
HspAI GCGC 1 cut(s) 250
KroI GCCGGC 1 cut(s) 854
KroNI GCCGGC 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 2 cut(s) 187, 676
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 1 cut(s) 519
LweI GCATC 2 cut(s) 357, 841
MaeI CTAG 2 cut(s) 221, 345
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 148, 235, 544
MalI GATC 2 cut(s) 189, 678
MboI GATC 2 cut(s) 187, 676
MhlI GDGCHC 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 583
MluNI TGGCCA 1 cut(s) 583
MlyI GAGTC 1 cut(s) 58
Mox20I TGGCCA 1 cut(s) 583
MroNI GCCGGC 1 cut(s) 854
MscI TGGCCA 1 cut(s) 583
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 2 cut(s) 212, 623
Msp20I TGGCCA 1 cut(s) 583
MspI CCGG 4 cut(s) 193, 420, 818, 855
MspR9I CCNGG 2 cut(s) 462, 818
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 6 cut(s) 401, 425, 589, 635, 638, 864
NaeI GCCGGC 1 cut(s) 856
NciI CCSGG 1 cut(s) 818
NdeII GATC 2 cut(s) 187, 676
NgoMIV GCCGGC 1 cut(s) 854
NlaIII CATG 5 cut(s) 217, 516, 622, 740, 879
NlaIV GGNNCC 2 cut(s) 424, 491
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 148, 235
NspV TTCGAA 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 856
PfeI GAWTC 4 cut(s) 217, 262, 447, 595
PflMI CCANNNNNTGG 1 cut(s) 560
PkrI GCNGC 7 cut(s) 166, 427, 534, 631, 634, 637, 640
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 424, 491
PspPI GGNCC 2 cut(s) 322, 771
RsaI GTAC 3 cut(s) 329, 574, 815
RsaNI GTAC 3 cut(s) 328, 573, 814
RseI CAYNNNNRTG 2 cut(s) 212, 623
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 7 cut(s) 165, 426, 533, 630, 633, 636, 639
Sau3AI GATC 2 cut(s) 187, 676
Sau96I GGNCC 2 cut(s) 322, 771
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 2 cut(s) 462, 818
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 357, 841
SfuI TTCGAA 1 cut(s) 450
SgrAI CRCCGGYG 1 cut(s) 419
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 212, 623
SmlI CTYRAG 3 cut(s) 269, 611, 775
SmoI CTYRAG 3 cut(s) 269, 611, 775
SsiI CCGC 7 cut(s) 165, 426, 629, 632, 635, 638, 740
SspMI CTAG 2 cut(s) 221, 345
StyD4I CCNGG 2 cut(s) 460, 816
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 450
TaqII GACCGA 2 cut(s) 403, 822
TatI WGTACW 2 cut(s) 327, 572
TauI GCSGC 6 cut(s) 167, 428, 632, 635, 638, 641
TfiI GAWTC 4 cut(s) 217, 262, 447, 595
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 2 cut(s) 411, 679
TseFI GTSAC 2 cut(s) 148, 235
TseI GCWGC 1 cut(s) 532
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 5 cut(s) 230, 244, 392, 636, 725
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 2 cut(s) 411, 679
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 322
XspI CTAG 2 cut(s) 221, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.