Rw1G015450

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001702
Physical Location & Seq
Reverse (-)
34474730 .. 34475056
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015450.1

Sequence Viewer

Length: 327 bp
ATGACCAACACAAGGCGCTTCATCAAGAATCTTCTAAAGAACGAGGAGCTTGACAAGTTCGTAAGGATGTGTTTGGATGACTGTTTGGACCTTTACACAGACGCCATAACTAGCATCAAGCAAGCCGTCAAGGATTACAAAATGAAGGACAACAGACACGCCAATCTCGCAGTGAGTTCGGTCGCCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGACACCAGGGTTGGTTTCGCCATTAACAAAGAGGAACAACGAGAGGTCTCAGCTGTGTGATATAGCGCTTATGATTATCAATATGGTCAGTTCGTCGTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

108

Amino Acids

12.2

Weight (kDa)

5.77

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 99 4.6e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AciI CCGC 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 102
AfeI AGCGCT 1 cut(s) 288
AfiI CCNNNNNNNGG 1 cut(s) 12
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 2 cut(s) 49, 274
AluI AGCT 2 cut(s) 49, 274
Alw26I GTCTC 2 cut(s) 215, 273
Aor51HI AGCGCT 1 cut(s) 288
AspLEI GCGC 2 cut(s) 18, 289
AspS9I GGNCC 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 88
BanI GGYRCC 1 cut(s) 188
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 215, 273
BfaI CTAG 1 cut(s) 111
BfoI RGCGCY 2 cut(s) 19, 290
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 227
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse118I RCCGGY 1 cut(s) 185
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 72, 82
BseLI CCNNNNNNNGG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 215, 273
Bso31I GGTCTC 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 216
BspACI CCGC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 227
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 72, 82
BstH2I RGCGCY 2 cut(s) 19, 290
BstHHI GCGC 2 cut(s) 18, 289
BstMAI GTCTC 2 cut(s) 215, 273
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 2 cut(s) 167, 191
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 72, 82
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 2 cut(s) 18, 289
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 1 cut(s) 110
CviJI RGCY 3 cut(s) 49, 125, 274
CviKI_1 RGCY 3 cut(s) 49, 125, 274
DdeI CTNAG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 88
Eco47III AGCGCT 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 226
FaiI YATR 4 cut(s) 107, 284, 293, 305
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 2 cut(s) 79, 89
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 111
GlaI GCGC 2 cut(s) 17, 288
GluI GCNGC 1 cut(s) 192
HaeII RGCGCY 2 cut(s) 19, 290
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 18, 289
Hin1I GRCGYC 1 cut(s) 102
Hin6I GCGC 2 cut(s) 16, 287
HinP1I GCGC 2 cut(s) 16, 287
HinfI GANTC 2 cut(s) 28, 213
HpaII CCGG 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 191
HpyF3I CTNAG 1 cut(s) 270
Hsp92I GRCGYC 1 cut(s) 102
HspAI GCGC 2 cut(s) 16, 287
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 3 cut(s) 199, 213, 240
LweI GCATC 1 cut(s) 123
MaeI CTAG 1 cut(s) 111
MboII GAAGA 3 cut(s) 23, 218, 230
MnlI CCTC 3 cut(s) 37, 246, 258
MseI TTAA 2 cut(s) 245, 325
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 167, 191
NlaIV GGNNCC 1 cut(s) 190
NspV TTCGAA 1 cut(s) 216
PfeI GAWTC 2 cut(s) 28, 213
PkrI GCNGC 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 274
SaqAI TTAA 2 cut(s) 245, 325
SatI GCNGC 1 cut(s) 192
Sau96I GGNCC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 228
SetI ASST 4 cut(s) 51, 93, 269, 276
SfaNI GCATC 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 88
SsiI CCGC 1 cut(s) 192
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 83
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 169
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 28, 213
Tru1I TTAA 2 cut(s) 245, 325
Tru9I TTAA 2 cut(s) 245, 325
TscAI CASTG 1 cut(s) 177
TspDTI ATGAA 2 cut(s) 10, 158
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 88
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.