Rh7BG230300

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001702
Physical Location & Seq
Forward (+)
20128237 .. 20129626
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG230300.1

Sequence Viewer

Length: 612 bp
ATGCACCATATTTCTTTCTTTTCAATCTCTTTATGCTGCTGCTTATTTTTCCTCATAATTCCTCATGAAAATTTCTCACCAATTACTGCAATATCTGTTGATATAATCAACCAAACCTGCAGAAGATGTGCCGATGAATCCCACGTCTTTAGCTATGACCTATGCACAACCTCTCTCCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAACTAGCACTAAACAATGCAAGCAACACGCTTCAGATCATAAAAAATTATTTGTTGATGAGCAACGAAAGTCTAGATCCTTTTGTTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATGCTGAAGGAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTACGGAAGCATCGAGTACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGCCATTTCGTAGTTGGCCAGAGAACTGAAAGGAAATACAATCAATCTGCCGGACTGAGGAAAACCCAGAATGAAGCTGTAAATTCCACATCATTCAAGCATGTATCCTCAGCTGGGAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

22.35

Weight (kDa)

5.17

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 173 7.3e-22 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AclWI GGATC 2 cut(s) 305, 371
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 2 cut(s) 70, 568
AcuI CTGAAG 2 cut(s) 251, 405
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 2 cut(s) 543, 600
AflIII ACRYGT 1 cut(s) 458
AgsI TTSAA 3 cut(s) 24, 334, 583
AjiI CACGTC 1 cut(s) 145
AluBI AGCT 4 cut(s) 153, 349, 563, 599
AluI AGCT 4 cut(s) 153, 349, 563, 599
AlwI GGATC 2 cut(s) 305, 371
AoxI GGCC 2 cut(s) 487, 502
ApeKI GCWGC 3 cut(s) 36, 39, 346
ApoI RAATTY 2 cut(s) 70, 568
AsuHPI GGTGA 2 cut(s) 69, 413
BalI TGGCCA 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 595
BbvI GCAGC 3 cut(s) 23, 26, 358
BciVI GTATCC 1 cut(s) 601
BfaI CTAG 3 cut(s) 218, 239, 308
BfmI CTRYAG 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 125
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 3 cut(s) 37, 40, 347
BlsI GCNGC 3 cut(s) 38, 41, 348
BmgBI CACGTC 1 cut(s) 145
BmsI GCATC 2 cut(s) 370, 458
BpmI CTGGAG 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 595
BsaJI CCNNGG 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 2 cut(s) 543, 600
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 543, 600
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 533, 609
BseNI ACTGG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 23, 26, 358
BseYI CCCAGC 1 cut(s) 599
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 2 cut(s) 489, 504
BsiSI CCGG 2 cut(s) 188, 537
BslI CCNNNNNNNGG 2 cut(s) 543, 600
BsnI GGCC 2 cut(s) 489, 504
Bsp143I GATC 3 cut(s) 270, 310, 363
BspANI GGCC 2 cut(s) 489, 504
BspCNI CTCAG 2 cut(s) 534, 608
BspHI TCATGA 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 122
BspMI ACCTGC 1 cut(s) 125
BspPI GGATC 2 cut(s) 305, 371
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 3 cut(s) 270, 310, 363
BssT1I CCWWGG 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 256, 344
BstDEI CTNAG 3 cut(s) 431, 542, 595
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 3 cut(s) 273, 313, 366
BstMBI GATC 3 cut(s) 270, 310, 363
BstMWI GCNNNNNNNGC 2 cut(s) 227, 389
BstNSI RCATGY 2 cut(s) 462, 590
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 23, 26, 358
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 2 cut(s) 489, 504
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 371
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 256, 344
CciI TCATGA 1 cut(s) 64
Csp6I GTAC 1 cut(s) 456
CviAII CATG 4 cut(s) 65, 197, 459, 587
CviJI RGCY 9 cut(s) 153, 195, 203, 349, 471, 489, 504, 563, 599
CviKI_1 RGCY 9 cut(s) 153, 195, 203, 349, 471, 489, 504, 563, 599
CviQI GTAC 1 cut(s) 456
DdeI CTNAG 3 cut(s) 431, 542, 595
DpnI GATC 3 cut(s) 272, 312, 365
DpnII GATC 3 cut(s) 270, 310, 363
EaeI YGGCCR 1 cut(s) 502
Eco130I CCWWGG 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 251, 405
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 4 cut(s) 68, 200, 462, 590
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FatI CATG 4 cut(s) 64, 196, 458, 586
Fnu4HI GCNGC 3 cut(s) 37, 40, 347
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 3 cut(s) 37, 40, 347
FspBI CTAG 3 cut(s) 218, 239, 308
GluI GCNGC 3 cut(s) 37, 40, 347
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 2 cut(s) 489, 504
HapII CCGG 2 cut(s) 188, 537
Hin1II CATG 4 cut(s) 68, 200, 462, 590
HinfI GANTC 1 cut(s) 137
HpaII CCGG 2 cut(s) 188, 537
HphI GGTGA 2 cut(s) 69, 413
Hpy188I TCNGA 2 cut(s) 270, 358
Hpy188III TCNNGA 2 cut(s) 65, 308
HpyAV CCTTC 6 cut(s) 328, 380, 461, 475, 479, 598
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 7 cut(s) 4, 89, 120, 165, 254, 346, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 389
HpyF3I CTNAG 3 cut(s) 431, 542, 595
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 4 cut(s) 68, 200, 462, 590
Kzo9I GATC 3 cut(s) 270, 310, 363
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 8 cut(s) 130, 201, 359, 383, 518, 550, 566, 585
Lsp1109I GCAGC 3 cut(s) 23, 26, 358
LweI GCATC 2 cut(s) 370, 458
MaeI CTAG 3 cut(s) 218, 239, 308
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 439
MalI GATC 3 cut(s) 272, 312, 365
MboI GATC 3 cut(s) 270, 310, 363
MboII GAAGA 1 cut(s) 135
MflI RGATCY 1 cut(s) 310
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 5 cut(s) 57, 70, 81, 280, 568
MluNI TGGCCA 1 cut(s) 504
MnlI CCTC 7 cut(s) 62, 72, 181, 370, 457, 537, 604
Mox20I TGGCCA 1 cut(s) 504
MscI TGGCCA 1 cut(s) 504
MseI TTAA 2 cut(s) 474, 610
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 2 cut(s) 188, 537
MwoI GCNNNNNNNGC 2 cut(s) 227, 389
NdeII GATC 3 cut(s) 270, 310, 363
NlaIII CATG 4 cut(s) 68, 200, 462, 590
NspI RCATGY 2 cut(s) 462, 590
PagI TCATGA 1 cut(s) 64
PciI ACATGT 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 38, 41, 348
PscI ACATGT 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
SaqAI TTAA 2 cut(s) 474, 610
SatI GCNGC 3 cut(s) 37, 40, 347
Sau3AI GATC 3 cut(s) 270, 310, 363
SetI ASST 8 cut(s) 119, 147, 155, 162, 173, 351, 565, 601
SfaNI GCATC 2 cut(s) 370, 458
SfcI CTRYAG 1 cut(s) 118
Sse9I AATT 5 cut(s) 57, 70, 81, 280, 568
SspI AATATT 1 cut(s) 424
SspMI CTAG 3 cut(s) 218, 239, 308
StyI CCWWGG 1 cut(s) 211
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 452
TasI AATT 5 cut(s) 57, 70, 81, 280, 568
TatI WGTACW 1 cut(s) 455
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 2 cut(s) 474, 610
Tru9I TTAA 2 cut(s) 474, 610
TscAI CASTG 1 cut(s) 378
TseI GCWGC 3 cut(s) 36, 39, 346
TspDTI ATGAA 3 cut(s) 81, 150, 573
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 1 cut(s) 378
XapI RAATTY 2 cut(s) 70, 568
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 2 cut(s) 462, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XspI CTAG 3 cut(s) 218, 239, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.