MD02G1225200.v1.1

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001702
Physical Location & Seq
Forward (+)
26406467 .. 26407027
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1225200.v1.1.491

Sequence Viewer

Length: 561 bp
ATGAGAACTCCCGCATTCTTTTTCACTTCTCTCCCTGTCTCCCTCCTCTTCTTCCTCTTCACCTTCAATGCCATAACTGCCAACAACCTCATCCCTGAAACTTGCAAAAACTTTGCTCAATACGATCCAAACCTAAGCTACAATTTCTGTGTAAACTCTCTGCAAGCAGCGCCGAAAAGCCAGCGCGCCGGTCTTCACAAACTCGGCATAATCTCAATCAAGTTAGTCCGCAACAACGCAACGGACACGAGGCATTACATCAAACACCTTCTGAAAAACAAGAACCTCGACCCCTACGTAAGCGCGTGTTTGGGCGACTGCTTGGAGCTTTACTCGGATGCCATACCAACCATTAAGCACGCCCTCATGAATTACAAGTCCAAGAAATACGAAGATGCCAACACTGAAGTGAGTTCAGTCATTGACGCCTCCACAACTTGTGAAGATGGATTCGACGAAGGAAATCAGTTGGTTTCGCCATTAACGAAGCGAAACAGCGACATGTTTCAGTTGTCGATTATAGCACTGTCTTTTATCAATATGCATAATTCACTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

187

Amino Acids

20.88

Weight (kDa)

7.65

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 177 2e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 186, 305
AciI CCGC 2 cut(s) 12, 229
AclWI GGATC 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 426
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 1 cut(s) 67
AleI CACNNNNGTG 1 cut(s) 407
AluBI AGCT 2 cut(s) 138, 328
AluI AGCT 2 cut(s) 138, 328
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 167
AspLEI GCGC 4 cut(s) 172, 186, 188, 305
AsuHPI GGTGA 1 cut(s) 52
BauI CACGAG 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 179
BccI CCATC 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 43
BfoI RGCGCY 1 cut(s) 173
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmsI GCATC 2 cut(s) 328, 385
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu10I CCTNAGC 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 426
Bse118I RCCGGY 1 cut(s) 188
BseGI GGATG 2 cut(s) 90, 343
BsePI GCGCGC 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 179
Bsh1236I CGCG 2 cut(s) 186, 305
BsiSI CCGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 14
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 2 cut(s) 12, 229
BspFNI CGCG 2 cut(s) 186, 305
BspHI TCATGA 1 cut(s) 366
BspPI GGATC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssHII GCGCGC 1 cut(s) 184
BssMI GATC 1 cut(s) 124
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 247
Bst2BI CACGAG 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 528
Bst6I CTCTTC 2 cut(s) 53, 62
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 165, 182, 186, 360
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 90, 343
BstFNI CGCG 2 cut(s) 186, 305
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 4 cut(s) 172, 186, 188, 305
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 77, 169
BstNSI RCATGY 1 cut(s) 505
BstSNI TACGTA 1 cut(s) 298
BstUI CGCG 2 cut(s) 186, 305
BstV1I GCAGC 1 cut(s) 179
BstV2I GAAGAC 1 cut(s) 185
BtsCI GGATG 2 cut(s) 90, 343
BtsIMutI CAGTG 2 cut(s) 402, 524
Cac8I GCNNGC 4 cut(s) 165, 182, 186, 360
CciI TCATGA 1 cut(s) 366
CfoI GCGC 4 cut(s) 172, 186, 188, 305
Cfr10I RCCGGY 1 cut(s) 188
CseI GACGC 1 cut(s) 434
CviAII CATG 2 cut(s) 367, 502
CviJI RGCY 3 cut(s) 138, 180, 328
CviKI_1 RGCY 3 cut(s) 138, 180, 328
DdeI CTNAG 1 cut(s) 134
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eam1104I CTCTTC 2 cut(s) 53, 62
EarI CTCTTC 2 cut(s) 53, 62
Eco105I TACGTA 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 546
FaeI CATG 2 cut(s) 370, 505
FaiI YATR 8 cut(s) 74, 209, 344, 368, 503, 521, 542, 546
FatI CATG 2 cut(s) 366, 501
FauI CCCGC 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 2 cut(s) 77, 350
Fsp4HI GCNGC 1 cut(s) 168
GlaI GCGC 4 cut(s) 171, 185, 187, 304
GluI GCNGC 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 173
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 434
HhaI GCGC 4 cut(s) 172, 186, 188, 305
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 2 cut(s) 370, 505
Hin6I GCGC 4 cut(s) 170, 184, 186, 303
HinP1I GCGC 4 cut(s) 170, 184, 186, 303
HinfI GANTC 1 cut(s) 450
HpaII CCGG 1 cut(s) 189
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 273, 337
Hpy188III TCNNGA 1 cut(s) 367
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 3 cut(s) 73, 278, 452
HpyCH4III ACNGT 1 cut(s) 528
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 105, 163, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 169
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 297
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 2 cut(s) 370, 505
HspAI GCGC 4 cut(s) 170, 184, 186, 303
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 4 cut(s) 48, 108, 194, 202
Lsp1109I GCAGC 1 cut(s) 179
LweI GCATC 2 cut(s) 328, 385
MaeII ACGT 1 cut(s) 297
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 6 cut(s) 40, 43, 49, 185, 404, 455
MluCI AATT 3 cut(s) 142, 370, 547
MnlI CCTC 8 cut(s) 53, 56, 65, 98, 243, 296, 374, 439
Mph1103I ATGCAT 1 cut(s) 546
MseI TTAA 2 cut(s) 354, 482
MslI CAYNNNNRTG 1 cut(s) 407
MspI CCGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 14
MvnI CGCG 2 cut(s) 186, 305
MwoI GCNNNNNNNGC 2 cut(s) 77, 169
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 370, 505
NmeAIII GCCGAG 1 cut(s) 183
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 1 cut(s) 505
OliI CACNNNNGTG 1 cut(s) 407
PagI TCATGA 1 cut(s) 366
PauI GCGCGC 1 cut(s) 184
PciI ACATGT 1 cut(s) 501
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 482
PctI GAATGC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 450
PkrI GCNGC 1 cut(s) 169
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 501
PteI GCGCGC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 354, 482
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 1 cut(s) 124
SetI ASST 8 cut(s) 65, 90, 135, 140, 270, 288, 300, 330
SfaNI GCATC 2 cut(s) 328, 385
SmiMI CAYNNNNRTG 1 cut(s) 407
SnaBI TACGTA 1 cut(s) 298
Sse9I AATT 3 cut(s) 142, 370, 547
SsiI CCGC 2 cut(s) 12, 229
TaaI ACNGT 1 cut(s) 528
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 288, 453, 515
TasI AATT 3 cut(s) 142, 370, 547
TfiI GAWTC 1 cut(s) 450
Tru1I TTAA 2 cut(s) 354, 482
Tru9I TTAA 2 cut(s) 354, 482
TscAI CASTG 2 cut(s) 409, 531
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 257
TspRI CASTG 2 cut(s) 409, 531
XceI RCATGY 1 cut(s) 505
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.