RchiOBHm_Chr1g0344001

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001702
Physical Location & Seq
Reverse (-)
36401407 .. 36402088
682 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57049

Sequence Viewer

Length: 561 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTTTCTTCCTCCTCTTCTCATCAACGTTGACTCCCATAACAGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCAAAAAGACCCCAACCTGAGCTACAAGTTTTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCAAGACGCCGATCTCCGGCAACTCGGCCTCATCTCCATGAATCTAGTCCAGCACAACGTGACCAACACAAGGCGCTTCATCAAGCATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGGTGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCTAGCATCAAGCAAGCTGTCAAGGATTACAAAATGAAGGACAACAGACACGCCAATATCGCAGTGAGTTCGGTCGCCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACCACGAGATGTTTCAGCTGTGTGTTTTAGCGCTTCTGATTATCAATATGGTCAGTTCGCCGTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

186

Amino Acids

20.99

Weight (kDa)

7.6

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 176 3.2e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 422
AciI CCGC 2 cut(s) 165, 426
AclI AACGTT 1 cut(s) 59
AcyI GRCGYC 2 cut(s) 183, 336
AdeI CACNNNGTG 1 cut(s) 235
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 4 cut(s) 135, 283, 359, 508
AluI AGCT 4 cut(s) 135, 283, 359, 508
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 451
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 202
AspLEI GCGC 2 cut(s) 252, 523
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 422
BauI CACGAG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 1 cut(s) 437
BceAI ACGGC 1 cut(s) 535
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 221, 345
BfoI RGCGCY 2 cut(s) 253, 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 2 cut(s) 165, 426
BlsI GCNGC 2 cut(s) 166, 427
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 424, 491
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 271, 357
Bpu10I CCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 183, 336
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
Bse118I RCCGGY 1 cut(s) 419
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 2 cut(s) 35, 293
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 422
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 193, 420
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 451
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 2 cut(s) 165, 426
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 424, 491
BspMI ACCTGC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 1 cut(s) 187
BssNI GRCGYC 2 cut(s) 183, 336
BssSI CACGAG 1 cut(s) 494
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 3 cut(s) 53, 447, 479
BstACI GRCGYC 2 cut(s) 183, 336
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 1 cut(s) 131
BstF5I GGATG 1 cut(s) 316
BstH2I RGCGCY 2 cut(s) 253, 524
BstHHI GCGC 2 cut(s) 252, 523
BstKTI GATC 1 cut(s) 190
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 1 cut(s) 187
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 2 cut(s) 401, 425
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 357
CfoI GCGC 2 cut(s) 252, 523
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 322
CseI GACGC 2 cut(s) 191, 344
CviAII CATG 1 cut(s) 214
CviJI RGCY 8 cut(s) 77, 135, 164, 177, 204, 283, 359, 508
CviKI_1 RGCY 8 cut(s) 77, 135, 164, 177, 204, 283, 359, 508
DdeI CTNAG 1 cut(s) 131
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DraIII CACNNNGTG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 53, 447, 479
EarI CTCTTC 3 cut(s) 53, 447, 479
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 322
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 1 cut(s) 217
FaiI YATR 5 cut(s) 14, 71, 215, 341, 539
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 165, 426
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 165, 426
FspBI CTAG 2 cut(s) 221, 345
GlaI GCGC 2 cut(s) 251, 522
GluI GCNGC 2 cut(s) 165, 426
HaeII RGCGCY 2 cut(s) 253, 524
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 193, 420
HgaI GACGC 2 cut(s) 191, 344
HhaI GCGC 2 cut(s) 252, 523
Hin1I GRCGYC 2 cut(s) 183, 336
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 2 cut(s) 250, 521
HinP1I GCGC 2 cut(s) 250, 521
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 3 cut(s) 64, 217, 447
HpaII CCGG 2 cut(s) 193, 420
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 2 cut(s) 94, 528
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 63
HpyAV CCTTC 2 cut(s) 31, 373
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 59, 234
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 425
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 59, 234
Hsp92I GRCGYC 2 cut(s) 183, 336
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 2 cut(s) 250, 521
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 7 cut(s) 112, 143, 206, 239, 433, 447, 474
LweI GCATC 2 cut(s) 271, 357
MaeI CTAG 2 cut(s) 221, 345
MaeII ACGT 2 cut(s) 59, 234
MaeIII GTNAC 2 cut(s) 148, 235
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MboII GAAGA 6 cut(s) 31, 40, 257, 452, 464, 496
MhlI GDGCHC 1 cut(s) 115
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 6 cut(s) 41, 53, 56, 215, 271, 480
MseI TTAA 2 cut(s) 479, 559
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 2 cut(s) 193, 420
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 401, 425
NdeII GATC 1 cut(s) 187
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 2 cut(s) 424, 491
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 148, 235
NspV TTCGAA 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PfeI GAWTC 2 cut(s) 217, 447
PkrI GCNGC 2 cut(s) 166, 427
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Psp1406I AACGTT 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 424, 491
PspPI GGNCC 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 479, 559
SatI GCNGC 2 cut(s) 165, 426
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 357
SfuI TTCGAA 1 cut(s) 450
SgrAI CRCCGGYG 1 cut(s) 419
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SsiI CCGC 2 cut(s) 165, 426
SspMI CTAG 2 cut(s) 221, 345
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 62, 237
TaqI TCGA 1 cut(s) 450
TaqII GACCGA 1 cut(s) 403
TauI GCSGC 2 cut(s) 167, 428
TfiI GAWTC 2 cut(s) 217, 447
Tru1I TTAA 2 cut(s) 479, 559
Tru9I TTAA 2 cut(s) 479, 559
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 148, 235
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 3 cut(s) 230, 244, 392
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 322
XspI CTAG 2 cut(s) 221, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.