MD06G1041900.v1.1

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001702
Physical Location & Seq
Reverse (-)
5550174 .. 5554864
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1041900.v1.1.491

Sequence Viewer

Length: 708 bp
ATGCATCGCATTTCTTCCTTTTCAATCTCTTTGTGCGGCTTCTTGTTTTTCCTGATGATTCCTCACGAAAAACTCTCCGCCGTTGCAATATCCGTTGACATAATCACCCAAACCTGCGAAAAATGCGCAGCTGAATCCGACGTGCTTAGCTACAACGTTTGTGCAGCTTCTCTCCAAGCAGTGCCTGTCAGCCATGTGACTAACCTTCAAGGACTGGCAGTGATAGCAATGGAGCTGGCACTGCACAATGCAACCAACACGCTTTCGACCATAAAGGAGTTGTCGAATAACAAAAGTTTGGATCCTTTTGCTCTGGTTTGCTTGAAGGATTGCTTGCAGCTATACTCGGACGCGATCACAACGTTGGCGGATGCGATCGGAGCATTTCTGAGTGAGGATTACGGCACGGCGAATACCTGGGTGAGTACAGTCATGGAAGCAACAACTACATGTGAGGGAGGGTTTAAGGAGAAGGAAGGTGAAGTGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAGTTATGTGATATTGTACTCTGCATAAGTAACTTGCTTAATTACTCAAAGTGGAATAAGAGCTCTGGAATTATCTGGATTTTTAATTCTTCAGTACATGATTTTTTATTTATTGCACAAGCGATATTGATGGAAAGGAAAATTGAGCTTGGAATTCGGGTGCAAGGGGTAAATGTCTTAGCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

236

Amino Acids

25.69

Weight (kDa)

4.84

Isoelectric Point (pI)

24.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 182 7.5e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 127
Acc36I ACCTGC 1 cut(s) 122
AccII CGCG 1 cut(s) 353
AciI CCGC 3 cut(s) 36, 78, 368
AclI AACGTT 2 cut(s) 156, 362
AclWI GGATC 2 cut(s) 296, 309
AcsI RAATTY 1 cut(s) 672
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 3 cut(s) 427, 537, 615
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 3 cut(s) 24, 209, 325
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 1 cut(s) 416
AluBI AGCT 7 cut(s) 131, 150, 167, 235, 340, 582, 667
AluI AGCT 7 cut(s) 131, 150, 167, 235, 340, 582, 667
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 2 cut(s) 296, 309
AlwNI CAGNNNCTG 1 cut(s) 185
ApeKI GCWGC 3 cut(s) 128, 164, 337
ApoI RAATTY 1 cut(s) 672
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 97, 433, 491
BamHI GGATCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 140, 176, 349
BccI CCATC 1 cut(s) 643
BceAI ACGGC 3 cut(s) 65, 418, 423
BciT130I CCWGG 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 122
BisI GCNGC 4 cut(s) 37, 129, 165, 338
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 4 cut(s) 38, 130, 166, 339
Bme1390I CCNGG 1 cut(s) 418
BmgBI CACGTC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 1 cut(s) 418
BmsI GCATC 2 cut(s) 13, 361
Bpu1102I GCTNAGC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 219
BseXI GCAGC 3 cut(s) 140, 176, 349
BsgI GTGCAG 2 cut(s) 183, 227
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 3 cut(s) 301, 354, 375
Bsp1720I GCTNAGC 1 cut(s) 146
BspACI CCGC 3 cut(s) 36, 78, 368
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 303
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 2 cut(s) 296, 309
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 3 cut(s) 301, 354, 375
Bst2UI CCWGG 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 237, 335
BstDEI CTNAG 3 cut(s) 146, 389, 697
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 353
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 3 cut(s) 304, 357, 378
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 3 cut(s) 301, 354, 375
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 4 cut(s) 123, 224, 241, 380
BstNI CCWGG 1 cut(s) 418
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 416
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 140, 176, 349
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 3 cut(s) 186, 225, 239
BtsIMutI CAGTG 3 cut(s) 186, 225, 239
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 237, 335
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 1 cut(s) 128
CseI GACGC 1 cut(s) 359
Csp6I GTAC 3 cut(s) 426, 536, 614
CviAII CATG 4 cut(s) 194, 433, 450, 617
CviQI GTAC 3 cut(s) 426, 536, 614
DdeI CTNAG 3 cut(s) 146, 389, 697
DpnI GATC 3 cut(s) 303, 356, 377
DpnII GATC 3 cut(s) 301, 354, 375
EciI GGCGGA 2 cut(s) 67, 383
Ecl136II GAGCTC 1 cut(s) 582
Eco24I GRGCYC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 584
FaeI CATG 4 cut(s) 197, 436, 453, 620
FaiI YATR 9 cut(s) 101, 195, 272, 343, 434, 451, 526, 545, 618
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 4 cut(s) 193, 432, 449, 616
Fnu4HI GCNGC 4 cut(s) 37, 129, 165, 338
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 4 cut(s) 37, 129, 165, 338
FspI TGCGCA 1 cut(s) 127
GlaI GCGC 1 cut(s) 127
GluI GCNGC 4 cut(s) 37, 129, 165, 338
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 128
Hin1II CATG 4 cut(s) 197, 436, 453, 620
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 58, 134
HphI GGTGA 3 cut(s) 97, 433, 491
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 4 cut(s) 139, 349, 380, 390
Hpy188III TCNNGA 4 cut(s) 52, 65, 585, 595
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 215, 319, 466, 470
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 3 cut(s) 141, 156, 362
HpyCH4V TGCA 9 cut(s) 4, 86, 164, 244, 251, 337, 543, 635, 682
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 224, 241, 380
HpyF3I CTNAG 3 cut(s) 146, 389, 697
HpySE526I ACGT 3 cut(s) 141, 156, 362
Hsp92II CATG 4 cut(s) 197, 436, 453, 620
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 3 cut(s) 301, 354, 375
LmnI GCTCC 2 cut(s) 232, 380
Lsp1109I GCAGC 3 cut(s) 140, 176, 349
LweI GCATC 2 cut(s) 13, 361
MaeII ACGT 3 cut(s) 141, 156, 362
MaeIII GTNAC 2 cut(s) 196, 548
MalI GATC 3 cut(s) 303, 356, 377
MboI GATC 3 cut(s) 301, 354, 375
MboII GAAGA 2 cut(s) 6, 600
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 584
MluCI AATT 6 cut(s) 505, 559, 588, 604, 660, 672
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 4 cut(s) 72, 388, 448, 452
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 465, 558, 603
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 418
MvaI CCWGG 1 cut(s) 418
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 4 cut(s) 123, 224, 241, 380
NdeII GATC 3 cut(s) 301, 354, 375
NlaIII CATG 4 cut(s) 197, 436, 453, 620
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 196
NsbI TGCGCA 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 453
PciI ACATGT 1 cut(s) 449
PfeI GAWTC 2 cut(s) 58, 134
PkrI GCNGC 4 cut(s) 38, 130, 166, 339
Ple19I CGATCG 1 cut(s) 378
PscI ACATGT 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 584
Psp1406I AACGTT 2 cut(s) 156, 362
Psp6I CCWGG 1 cut(s) 416
PspGI CCWGG 1 cut(s) 416
PspN4I GGNNCC 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 301
PvuI CGATCG 1 cut(s) 378
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 3 cut(s) 427, 537, 615
RsaNI GTAC 3 cut(s) 426, 536, 614
SacI GAGCTC 1 cut(s) 584
SaqAI TTAA 3 cut(s) 465, 558, 603
SatI GCNGC 4 cut(s) 37, 129, 165, 338
Sau3AI GATC 3 cut(s) 301, 354, 375
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 1 cut(s) 584
SfaNI GCATC 2 cut(s) 13, 361
Sse9I AATT 6 cut(s) 505, 559, 588, 604, 660, 672
SsiI CCGC 3 cut(s) 36, 78, 368
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 416
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 3 cut(s) 144, 159, 365
TaqI TCGA 2 cut(s) 266, 284
TasI AATT 6 cut(s) 505, 559, 588, 604, 660, 672
TatI WGTACW 3 cut(s) 425, 535, 613
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 58, 134
Tru1I TTAA 3 cut(s) 465, 558, 603
Tru9I TTAA 3 cut(s) 465, 558, 603
TscAI CASTG 3 cut(s) 186, 225, 246
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 3 cut(s) 128, 164, 337
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 3 cut(s) 186, 225, 246
XapI RAATTY 1 cut(s) 672
XceI RCATGY 1 cut(s) 453
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.