FvH4_5g25840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001799
Physical Location & Seq
Reverse (-)
17335622 .. 17339528
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25840.t1

Sequence Viewer

Length: 426 bp
ATGTCGAAGGGGAAGAAGGAATTGATGGCGTCGGCGCCGTGGAGGGTTGAAGAGGACGACGGCGGAGATAAGTTCTCGGACGCGAAGATGAAAGTGACACGGCAGCAGGCGGGGGCGGAGTCAACCATGCACGTCCCTGGCAAGAAAACCAAGCGCGCTGCTCACGACTACGACGCCGACGACTCTCTCACTGAGATCGACCCCGAGCTCCGCTACAGCTTCCACCGTAACTATCAGTTCCTTCAACGAGTTTTTAGCATTGATACTATCGTGAAACCTCTTCCGGCTTCTATGGCTTACAATGTCTCCCGCAACTTGAACTTCTTTACTCGCATTTTCACCCAGTTTTTTGATCCTGAAGGTGTTGCGAATGCGCAGAAATCACTCGGTATAGGACAGGAAGAGAAAGCTCGTCGCGTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

16.09

Weight (kDa)

9.15

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 3 cut(s) 83, 156, 417
AciI CCGC 6 cut(s) 63, 110, 116, 211, 310, 421
AclWI GGATC 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 3 cut(s) 29, 35, 174
AgsI TTSAA 3 cut(s) 50, 245, 319
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 3 cut(s) 208, 219, 410
AluI AGCT 3 cut(s) 208, 219, 410
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 103, 158
AspLEI GCGC 4 cut(s) 37, 156, 158, 376
AsuHPI GGTGA 1 cut(s) 331
AvaI CYCGRG 1 cut(s) 203
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 115, 145
BccI CCATC 1 cut(s) 19
BceAI ACGGC 3 cut(s) 22, 76, 116
BciT130I CCWGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 214
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 2 cut(s) 104, 159
BlsI GCNGC 2 cut(s) 105, 160
Bme1390I CCNGG 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
BsaHI GRCGYC 3 cut(s) 29, 35, 174
BsaJI CCNNGG 2 cut(s) 38, 136
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bse1I ACTGG 1 cut(s) 343
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 38, 136
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 343
BsePI GCGCGC 1 cut(s) 154
BseXI GCAGC 2 cut(s) 115, 145
Bsh1236I CGCG 3 cut(s) 83, 156, 417
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 195, 352
BspACI CCGC 6 cut(s) 63, 110, 116, 211, 310, 421
BspCNI CTCAG 1 cut(s) 184
BspFNI CGCG 3 cut(s) 83, 156, 417
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 34
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 38, 136
BssHII GCGCGC 1 cut(s) 154
BssMI GATC 2 cut(s) 195, 352
BssNI GRCGYC 3 cut(s) 29, 35, 174
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 3 cut(s) 45, 285, 396
BstACI GRCGYC 3 cut(s) 29, 35, 174
BstC8I GCNNGC 3 cut(s) 108, 156, 419
BstDEI CTNAG 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 38
BstFNI CGCG 3 cut(s) 83, 156, 417
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 4 cut(s) 37, 156, 158, 376
BstKTI GATC 2 cut(s) 198, 355
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 2 cut(s) 195, 352
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 214
BstUI CGCG 3 cut(s) 83, 156, 417
BstV1I GCAGC 2 cut(s) 115, 145
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 108, 156, 419
CfoI GCGC 4 cut(s) 37, 156, 158, 376
CseI GACGC 3 cut(s) 18, 89, 182
CviAII CATG 1 cut(s) 127
CviJI RGCY 5 cut(s) 208, 219, 287, 296, 410
CviKI_1 RGCY 5 cut(s) 208, 219, 287, 296, 410
DdeI CTNAG 1 cut(s) 192
DinI GGCGCC 1 cut(s) 36
DpnI GATC 2 cut(s) 197, 354
DpnII GATC 2 cut(s) 195, 352
Eam1104I CTCTTC 3 cut(s) 45, 285, 396
EarI CTCTTC 3 cut(s) 45, 285, 396
EciI GGCGGA 2 cut(s) 78, 131
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 210
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
FaeI CATG 1 cut(s) 130
FaiI YATR 3 cut(s) 128, 293, 392
FaqI GGGAC 1 cut(s) 119
FatI CATG 1 cut(s) 126
FauI CCCGC 2 cut(s) 103, 317
Fnu4HI GCNGC 2 cut(s) 104, 159
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 2 cut(s) 104, 159
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 4 cut(s) 36, 155, 157, 375
GluI GCNGC 2 cut(s) 104, 159
HaeII RGCGCY 1 cut(s) 38
HapII CCGG 1 cut(s) 284
HgaI GACGC 3 cut(s) 18, 89, 182
HhaI GCGC 4 cut(s) 37, 156, 158, 376
Hin1I GRCGYC 3 cut(s) 29, 35, 174
Hin1II CATG 1 cut(s) 130
Hin6I GCGC 4 cut(s) 35, 154, 156, 374
HinP1I GCGC 4 cut(s) 35, 154, 156, 374
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 2 cut(s) 119, 182
HpaII CCGG 1 cut(s) 284
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 3 cut(s) 164, 271, 356
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 5 cut(s) 34, 62, 176, 182, 417
HpyAV CCTTC 3 cut(s) 10, 251, 353
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 3 cut(s) 29, 35, 174
Hsp92II CATG 1 cut(s) 130
HspAI GCGC 4 cut(s) 35, 154, 156, 374
KasI GGCGCC 1 cut(s) 34
Kzo9I GATC 2 cut(s) 195, 352
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 7 cut(s) 92, 123, 150, 297, 356, 369, 383
Lsp1109I GCAGC 2 cut(s) 115, 145
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 94, 227
MalI GATC 2 cut(s) 197, 354
MboI GATC 2 cut(s) 195, 352
MboII GAAGA 5 cut(s) 25, 62, 97, 272, 413
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 1 cut(s) 20
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 2 cut(s) 128, 176
MnlI CCTC 3 cut(s) 36, 46, 288
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 3 cut(s) 83, 156, 417
MwoI GCNNNNNNNGC 1 cut(s) 293
NarI GGCGCC 1 cut(s) 35
NdeII GATC 2 cut(s) 195, 352
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 94
NsbI TGCGCA 1 cut(s) 375
PauI GCGCGC 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 376
PkrI GCNGC 2 cut(s) 105, 160
PleI GAGTC 2 cut(s) 127, 176
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 2 cut(s) 127, 176
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 36
PteI GCGCGC 1 cut(s) 154
SacI GAGCTC 1 cut(s) 210
SatI GCNGC 2 cut(s) 104, 159
Sau3AI GATC 2 cut(s) 195, 352
SchI GAGTC 2 cut(s) 128, 176
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 210
SetI ASST 6 cut(s) 135, 210, 221, 280, 364, 412
SfcI CTRYAG 1 cut(s) 214
SfoI GGCGCC 1 cut(s) 36
Sse9I AATT 1 cut(s) 20
SsiI CCGC 6 cut(s) 63, 110, 116, 211, 310, 421
SspDI GGCGCC 1 cut(s) 34
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 5, 198
TasI AATT 1 cut(s) 20
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 103, 158
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 1 cut(s) 104
TspRI CASTG 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.