MD08G1215600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001799
Physical Location & Seq
Forward (+)
27845439 .. 27848242
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215600.v1.1.491

Sequence Viewer

Length: 417 bp
ATGTCTAAGACGAAGAAGCAATTGATGTCATCAGCTCCGTGGAGGGTGGAAGAAGAGGCGGAAGCTGAATTCCCAGAAGGGAAGCTGAAAGTGACGAGCCAGCCAGGGTCGACTCCAACCATGCACGTCCCTCGCAAGAAAAACAAGCGCTCTCAAGACCAAGACGATCAAGATTCTCTTACTGAGATTGACCCTGAGCTCCGATACAGCTTCGAGCGCAACTTTCAGTTCCTTCAACGGGTGTTTAGCATTGACACTGTCGTGAAACCTCTTCCGCCTGCCATGGCATACAATGTCTCCCGCAACTTGAACTTCTTCACTCGTATCTTCACACAGTTCTTTGATCCTGAAGGTATTGCAAATGCGCAAAAGTCACTTGGGCTAGGACAGGAAGAAAAAGTTCGTAAAGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.97

Weight (kDa)

9.22

Isoelectric Point (pI)

60.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 366
AccI GTMKAC 1 cut(s) 110
AciI CCGC 3 cut(s) 59, 275, 301
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 369
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 2 cut(s) 236, 310
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 103
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 5 cut(s) 35, 65, 85, 199, 210
AluI AGCT 5 cut(s) 35, 65, 85, 199, 210
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 338
Aor51HI AGCGCT 1 cut(s) 149
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 314, 399
AspLEI GCGC 3 cut(s) 150, 219, 367
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 105
BmgBI CACGTC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 38, 104, 282
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 38, 104, 282
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 174, 186
BsiHKAI GWGCWC 1 cut(s) 201
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 166, 343
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 3 cut(s) 59, 275, 301
BspCNI CTCAG 2 cut(s) 175, 187
BspPI GGATC 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 38, 104, 282
BssMI GATC 2 cut(s) 166, 343
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 259, 336
Bst6I CTCTTC 2 cut(s) 48, 276
BstC8I GCNNGC 2 cut(s) 101, 279
BstDEI CTNAG 3 cut(s) 6, 183, 195
BstDSI CCRYGG 2 cut(s) 38, 282
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 219, 367
BstKTI GATC 2 cut(s) 169, 346
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 166, 343
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BtgI CCRYGG 2 cut(s) 38, 282
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 101, 279
CfoI GCGC 3 cut(s) 150, 219, 367
CviAII CATG 2 cut(s) 121, 283
CviJI RGCY 8 cut(s) 35, 65, 85, 99, 103, 199, 210, 382
CviKI_1 RGCY 8 cut(s) 35, 65, 85, 99, 103, 199, 210, 382
DdeI CTNAG 3 cut(s) 6, 183, 195
DpnI GATC 2 cut(s) 168, 345
DpnII GATC 2 cut(s) 166, 343
Eam1104I CTCTTC 2 cut(s) 48, 276
EarI CTCTTC 2 cut(s) 48, 276
EciI GGCGGA 2 cut(s) 74, 264
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 201
Eco47III AGCGCT 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 124, 286
FaiI YATR 3 cut(s) 122, 284, 289
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 120, 282
FauI CCCGC 1 cut(s) 308
FblI GTMKAC 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 1 cut(s) 383
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 3 cut(s) 149, 218, 366
HaeII RGCGCY 1 cut(s) 151
HhaI GCGC 3 cut(s) 150, 219, 367
Hin1II CATG 2 cut(s) 124, 286
Hin6I GCGC 3 cut(s) 148, 217, 365
HinP1I GCGC 3 cut(s) 148, 217, 365
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HinfI GANTC 2 cut(s) 112, 173
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 4 cut(s) 155, 170, 262, 347
Hpy8I GTNNAC 1 cut(s) 111
HpyAV CCTTC 3 cut(s) 71, 242, 344
HpyCH4III ACNGT 2 cut(s) 259, 336
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 124, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 3 cut(s) 6, 183, 195
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 124, 286
HspAI GCGC 3 cut(s) 148, 217, 365
Kzo9I GATC 2 cut(s) 166, 343
LmnI GCTCC 2 cut(s) 40, 204
LpnPI CCDG 8 cut(s) 87, 90, 113, 117, 207, 291, 360, 374
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 91, 372
MalI GATC 2 cut(s) 168, 345
MboI GATC 2 cut(s) 166, 343
MboII GAAGA 7 cut(s) 25, 62, 65, 263, 307, 319, 404
MfeI CAATTG 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 2 cut(s) 20, 68
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 4 cut(s) 36, 49, 141, 279
MroXI GAANNNNTTC 2 cut(s) 314, 399
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 282
NdeII GATC 2 cut(s) 166, 343
NlaIII CATG 2 cut(s) 124, 286
NmuCI GTSAC 2 cut(s) 91, 372
NsbI TGCGCA 1 cut(s) 366
OliI CACNNNNGTG 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 409
PdmI GAANNNNTTC 2 cut(s) 314, 399
PfeI GAWTC 1 cut(s) 173
PflFI GACNNNGTC 1 cut(s) 257
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PsyI GACNNNGTC 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 109
Sau3AI GATC 2 cut(s) 166, 343
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 201
SetI ASST 8 cut(s) 37, 67, 87, 129, 201, 212, 271, 355
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 20, 68
SsiI CCGC 3 cut(s) 59, 275, 301
SspMI CTAG 1 cut(s) 383
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 259, 336
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 110, 213
TasI AATT 2 cut(s) 20, 68
TfiI GAWTC 1 cut(s) 173
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 91, 372
Tsp45I GTSAC 2 cut(s) 91, 372
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 262
Tth111I GACNNNGTC 1 cut(s) 257
XapI RAATTY 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 2 cut(s) 314, 399
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.