Rh5BG107400

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001799
Physical Location & Seq
Forward (+)
10026580 .. 10027158
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG107400.1

Sequence Viewer

Length: 204 bp
ATGGCTTCGCGTCGGCTCTTATCCCCTCTTCTCCGATCCCCTTCCAGATTTTCGATCTCAAACCATAACCCTACCCTATCAACCCCCTCGCAATCGTCTCCCCGCGCTTCGCCGTACGCCCACCTCCTCCGGGATGCTCTTCTTACCGGCTGCCGCATGCTCCACACCTCTTCTTCTATCACCGTATACCTTGACTTTGCTTGA

Protein Analysis

67

Amino Acids

7.39

Weight (kDa)

11.27

Isoelectric Point (pI)

87.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Synthase_beta PF11421 1 - 43 1.2e-09 ATP synthase F1 beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 10, 105
AciI CCGC 2 cut(s) 103, 154
AclWI GGATC 1 cut(s) 30
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 130
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 172
BbvI GCAGC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 102
BisI GCNGC 2 cut(s) 151, 154
BlsI GCNGC 2 cut(s) 152, 155
Bme1390I CCNGG 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 131
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 1 cut(s) 146
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 137
Bsh1236I CGCG 2 cut(s) 10, 105
BsiSI CCGG 2 cut(s) 130, 147
BsiWI CGTACG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 102
BsmBI CGTCTC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 35, 54
BspACI CCGC 2 cut(s) 103, 154
BspFNI CGCG 2 cut(s) 10, 105
BspPI GGATC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 2 cut(s) 35, 54
BssNAI GTATAC 1 cut(s) 187
Bst1107I GTATAC 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 3 cut(s) 33, 144, 175
BstC8I GCNNGC 1 cut(s) 158
BstF5I GGATG 1 cut(s) 139
BstFNI CGCG 2 cut(s) 10, 105
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 38, 57
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 2 cut(s) 35, 54
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 2 cut(s) 10, 105
BstV1I GCAGC 1 cut(s) 137
BstZ17I GTATAC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 158
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 146
Csp6I GTAC 1 cut(s) 115
CviAII CATG 1 cut(s) 157
CviJI RGCY 3 cut(s) 5, 16, 150
CviKI_1 RGCY 3 cut(s) 5, 16, 150
CviQI GTAC 1 cut(s) 115
DpnI GATC 2 cut(s) 37, 56
DpnII GATC 2 cut(s) 35, 54
Eam1104I CTCTTC 3 cut(s) 33, 144, 175
EarI CTCTTC 3 cut(s) 33, 144, 175
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 1 cut(s) 160
FaiI YATR 3 cut(s) 66, 158, 187
FatI CATG 1 cut(s) 156
FauI CCCGC 1 cut(s) 110
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 151, 154
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 151, 154
GlaI GCGC 1 cut(s) 106
GluI GCNGC 2 cut(s) 151, 154
HapII CCGG 2 cut(s) 130, 147
HhaI GCGC 1 cut(s) 107
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HpaII CCGG 2 cut(s) 130, 147
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 35
Hpy188III TCNNGA 1 cut(s) 45
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 184
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 35, 54
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 3 cut(s) 58, 143, 160
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 124
MalI GATC 2 cut(s) 37, 56
MboI GATC 2 cut(s) 35, 54
MboII GAAGA 4 cut(s) 20, 131, 162, 165
MnlI CCTC 5 cut(s) 36, 97, 134, 137, 178
MspI CCGG 2 cut(s) 130, 147
MspR9I CCNGG 1 cut(s) 131
MvnI CGCG 2 cut(s) 10, 105
NciI CCSGG 1 cut(s) 131
NdeII GATC 2 cut(s) 35, 54
NlaIII CATG 1 cut(s) 160
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 144
Pfl23II CGTACG 1 cut(s) 114
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 2 cut(s) 152, 155
PspLI CGTACG 1 cut(s) 114
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 144
SatI GCNGC 2 cut(s) 151, 154
Sau3AI GATC 2 cut(s) 35, 54
ScrFI CCNGG 1 cut(s) 131
SetI ASST 3 cut(s) 126, 170, 192
SfaNI GCATC 1 cut(s) 124
SgeI CNNG 9 cut(s) 21, 57, 100, 114, 116, 142, 143, 159, 169
SphI GCATGC 1 cut(s) 160
SsiI CCGC 2 cut(s) 103, 154
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 53
TauI GCSGC 1 cut(s) 156
TseI GCWGC 1 cut(s) 150
XceI RCATGY 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.