pycom15g35870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001799
Physical Location & Seq
Forward (+)
35943833 .. 35945812
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35870.3

Sequence Viewer

Length: 417 bp
ATGTCGAAGTCGAAGAAGCAGTTGATGTCTTCAGCTCCGTGGAGGGTGGAACAAGAGGCGGAGGATGAATTCCCAGAAGGGAAGCTTAAGGTGACGAGCCAGCCAGGGTCGACTCCAACCATGCACGTCCCTCGCAAGAAAACCAAGCGCTCTCAGGACCAAGACGATCAAGATTCTCTCACGGAGATCGATCCTGAGCTCCGCTACAGCTTCCAGCGCAACTTTCAGTTCCTTCAACGGGTGTTCAGCATTGATACTGTCGTGAAACCTCTTCCTCCTGCCATGGCATACAATGTCTCCCGCAACTTGAACTTCTTCACTCGTATTTTCACACAGTTCTTTGATCCTGAAGGTATTGCAAATGCGCAAAAATCACTTGGGCTAGGACAGGAAGAAAAAGTTCGTAAAGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.98

Weight (kDa)

9.43

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 366
AccI GTMKAC 1 cut(s) 110
AciI CCGC 3 cut(s) 59, 202, 301
AclWI GGATC 2 cut(s) 185, 338
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 2 cut(s) 15, 369
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 238
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 2 cut(s) 236, 310
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 4 cut(s) 35, 85, 199, 210
AluI AGCT 4 cut(s) 35, 85, 199, 210
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 2 cut(s) 185, 338
Aor51HI AGCGCT 1 cut(s) 149
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 314, 399
AspLEI GCGC 3 cut(s) 150, 219, 367
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 201
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 383
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 151
BfrI CTTAAG 1 cut(s) 86
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 157
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 195
Bsa29I ATCGAT 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 38, 104, 282
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 38, 104, 282
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 167, 186
BshVI ATCGAT 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 201
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 4 cut(s) 166, 186, 190, 343
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 3 cut(s) 59, 202, 301
BspCNI CTCAG 2 cut(s) 166, 187
BspDI ATCGAT 1 cut(s) 189
BspPI GGATC 2 cut(s) 185, 338
BspTI CTTAAG 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 38, 104, 282
BssMI GATC 4 cut(s) 166, 186, 190, 343
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 259, 336
Bst6I CTCTTC 1 cut(s) 276
BstAFI CTTAAG 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 2 cut(s) 153, 195
BstDSI CCRYGG 2 cut(s) 38, 282
BstF5I GGATG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 219, 367
BstKTI GATC 4 cut(s) 169, 189, 193, 346
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 4 cut(s) 166, 186, 190, 343
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 205
BstV2I GAAGAC 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 189
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 2 cut(s) 38, 282
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 3 cut(s) 150, 219, 367
Cfr13I GGNCC 1 cut(s) 157
ClaI ATCGAT 1 cut(s) 189
CviAII CATG 2 cut(s) 121, 283
CviJI RGCY 7 cut(s) 35, 85, 99, 103, 199, 210, 382
CviKI_1 RGCY 7 cut(s) 35, 85, 99, 103, 199, 210, 382
DdeI CTNAG 2 cut(s) 153, 195
DpnI GATC 4 cut(s) 168, 188, 192, 345
DpnII GATC 4 cut(s) 166, 186, 190, 343
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 157
Eco47III AGCGCT 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 15, 369
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 124, 286
FaiI YATR 3 cut(s) 122, 284, 289
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 120, 282
FauI CCCGC 1 cut(s) 308
FblI GTMKAC 1 cut(s) 110
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 1 cut(s) 383
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 3 cut(s) 149, 218, 366
HaeII RGCGCY 1 cut(s) 151
HhaI GCGC 3 cut(s) 150, 219, 367
Hin1II CATG 2 cut(s) 124, 286
Hin6I GCGC 3 cut(s) 148, 217, 365
HinP1I GCGC 3 cut(s) 148, 217, 365
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 2 cut(s) 112, 173
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 111
Hpy188III TCNNGA 5 cut(s) 155, 170, 194, 262, 347
Hpy8I GTNNAC 1 cut(s) 111
HpyAV CCTTC 3 cut(s) 71, 242, 344
HpyCH4III ACNGT 2 cut(s) 259, 336
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 124, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 2 cut(s) 153, 195
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 124, 286
HspAI GCGC 3 cut(s) 148, 217, 365
Kzo9I GATC 4 cut(s) 166, 186, 190, 343
LmnI GCTCC 2 cut(s) 40, 204
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 91
MalI GATC 4 cut(s) 168, 188, 192, 345
MboI GATC 4 cut(s) 166, 186, 190, 343
MboII GAAGA 5 cut(s) 21, 25, 263, 307, 404
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 1 cut(s) 68
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 6 cut(s) 36, 49, 55, 141, 279, 285
MroXI GAANNNNTTC 2 cut(s) 314, 399
MseI TTAA 1 cut(s) 87
MspCI CTTAAG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 282
NdeII GATC 4 cut(s) 166, 186, 190, 343
NlaIII CATG 2 cut(s) 124, 286
NmuCI GTSAC 1 cut(s) 91
NsbI TGCGCA 1 cut(s) 366
PcsI WCGNNNNNNNCGW 1 cut(s) 409
PdmI GAANNNNTTC 2 cut(s) 314, 399
PfeI GAWTC 1 cut(s) 173
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspPI GGNCC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 109
SaqAI TTAA 1 cut(s) 87
Sau3AI GATC 4 cut(s) 166, 186, 190, 343
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 201
SetI ASST 8 cut(s) 37, 87, 93, 129, 201, 212, 271, 355
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 1 cut(s) 68
SsiI CCGC 3 cut(s) 59, 202, 301
SspMI CTAG 1 cut(s) 383
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 259, 336
TaiI ACGT 1 cut(s) 129
TaqI TCGA 4 cut(s) 5, 11, 110, 189
TasI AATT 1 cut(s) 68
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 2 cut(s) 27, 197
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 2 cut(s) 314, 399
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.