Rh4BG263700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001799
Physical Location & Seq
Forward (+)
43746633 .. 43748692
2060 bp
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UTR
Exon/CDS
Intron
Rh4BG263700.1

Sequence Viewer

Length: 354 bp
ATGGCGCTTGATTATTTGCATGCCAACCATATTCTTCATCGTCATGTCAAGTGTTCAAACATATTTTTGACAAAGGATCAAGACATACGTCTAGGTGATTTTGGTCTTGCTAAAATGTTGACTTCAGATGATCTAGCTTCCTCTGTTGTGGGAACTCCGAGTTATATGTGCCCTGAGCTTCTTGCCGACATACCATATGGTTCTAAGTCAGATATTTGGTCTCTAGGGTGCTGTATATATGAAATGGCCGCTCACAAGCCAGCATTTAAAGCCTTTGATATACAATCTTTGATCAACAAAATCAACAAGTCAATAGTGGCTCCACTTCCAACGGTTTACTGCTGTGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

117

Amino Acids

12.92

Weight (kDa)

6.48

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 101 5.4e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 109 7.1e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 251
AciI CCGC 1 cut(s) 249
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 108
AgsI TTSAA 1 cut(s) 57
AluBI AGCT 2 cut(s) 137, 178
AluI AGCT 2 cut(s) 137, 178
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 1 cut(s) 84
AoxI GGCC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 173
BclI TGATCA 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 3 cut(s) 92, 134, 224
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 225
BseMII CTCAG 1 cut(s) 165
BseSI GKGCMC 1 cut(s) 173
BshFI GGCC 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 3 cut(s) 76, 130, 291
BspACI CCGC 1 cut(s) 249
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 251
BssMI GATC 3 cut(s) 76, 130, 291
Bst4CI ACNGT 1 cut(s) 334
BstC8I GCNNGC 2 cut(s) 21, 261
BstDEI CTNAG 2 cut(s) 174, 204
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 79, 133, 294
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 3 cut(s) 76, 130, 291
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 23
BstPAI GACNNNNGTC 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 173
BsuRI GGCC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 21, 261
CfoI GCGC 1 cut(s) 7
CviAII CATG 2 cut(s) 20, 44
CviJI RGCY 6 cut(s) 137, 178, 248, 259, 272, 320
CviKI_1 RGCY 6 cut(s) 137, 178, 248, 259, 272, 320
DdeI CTNAG 2 cut(s) 174, 204
DpnI GATC 3 cut(s) 78, 132, 293
DpnII GATC 3 cut(s) 76, 130, 291
DraI TTTAAA 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 108
FaeI CATG 2 cut(s) 23, 47
FatI CATG 2 cut(s) 19, 43
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 3 cut(s) 92, 134, 224
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 249
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 248
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 23, 47
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 2 cut(s) 120, 337
Hpy188I TCNGA 3 cut(s) 127, 159, 211
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 2 cut(s) 120, 337
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 2 cut(s) 19, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 174, 204
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 2 cut(s) 23, 47
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 3 cut(s) 76, 130, 291
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 2 cut(s) 186, 273
MaeI CTAG 3 cut(s) 92, 134, 224
MaeII ACGT 1 cut(s) 88
MalI GATC 3 cut(s) 78, 132, 293
MbiI CCGCTC 1 cut(s) 251
MboI GATC 3 cut(s) 76, 130, 291
MboII GAAGA 1 cut(s) 26
MhlI GDGCHC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 1 cut(s) 151
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeI CATATG 1 cut(s) 196
NdeII GATC 3 cut(s) 76, 130, 291
NlaIII CATG 2 cut(s) 23, 47
NlaIV GGNNCC 1 cut(s) 321
NspI RCATGY 1 cut(s) 23
PaeI GCATGC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 250
PshAI GACNNNNGTC 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 76, 130, 291
SduI GDGCHC 1 cut(s) 173
SetI ASST 4 cut(s) 91, 97, 139, 180
SmiMI CAYNNNNRTG 1 cut(s) 42
SphI GCATGC 1 cut(s) 23
SsiI CCGC 1 cut(s) 249
SspMI CTAG 3 cut(s) 92, 134, 224
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 1 cut(s) 91
TauI GCSGC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TspDTI ATGAA 2 cut(s) 26, 255
XceI RCATGY 1 cut(s) 23
XspI CTAG 3 cut(s) 92, 134, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.