Rmu_sc0006888.1_g000018

SDA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006888.1
OG0001799
Physical Location & Seq
Reverse (-)
67896 .. 69547
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006888.1_g000018.1.cds

Sequence Viewer

Length: 279 bp
atgcatgtccctcgcaagcgcgctcaggactatgaccacgacgactctcttactgagatcgaccctgagatccgctacagcttccactgtaactatcagctccatgtaaaaagaagaatgaccaaggaggagaggctggcattagtgaaagcagggtgggaggaaagagggaagtacatggctagagctgctgtaaaacagaagaaggagggaggctcgagcaataagcagaagcaacaaaagtatgccacttgctgcaaaaagggcaaaggcagctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC130_3NT SDA1

Protein Analysis

92

Amino Acids

10.79

Weight (kDa)

9.71

Isoelectric Point (pI)

71.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AciI CCGC 1 cut(s) 73
AclWI GGATC 1 cut(s) 64
AfaI GTAC 1 cut(s) 176
AluBI AGCT 4 cut(s) 81, 100, 188, 276
AluI AGCT 4 cut(s) 81, 100, 188, 276
AlwI GGATC 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 217
ApeKI GCWGC 3 cut(s) 188, 255, 273
AspLEI GCGC 2 cut(s) 21, 23
AvaI CYCGRG 1 cut(s) 217
BbvI GCAGC 2 cut(s) 175, 242
BcgI CGANNNNNNTGC 1 cut(s) 27
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 1 cut(s) 76
BisI GCNGC 3 cut(s) 189, 256, 274
BlsI GCNGC 3 cut(s) 190, 257, 275
BmeT110I CYCGRG 1 cut(s) 217
BplI GAGNNNNNCTC 2 cut(s) 200, 232
Bpu10I CCTNAGC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 123
BseMII CTCAG 3 cut(s) 38, 45, 57
BsePI GCGCGC 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 175, 242
Bsh1236I CGCG 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 217
Bsp143I GATC 2 cut(s) 57, 69
BspACI CCGC 1 cut(s) 73
BspCNI CTCAG 3 cut(s) 37, 46, 58
BspFNI CGCG 1 cut(s) 21
BspPI GGATC 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 123
BssHII GCGCGC 1 cut(s) 19
BssMI GATC 2 cut(s) 57, 69
BssT1I CCWWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 89
BstC8I GCNNGC 3 cut(s) 17, 21, 138
BstDEI CTNAG 3 cut(s) 24, 54, 66
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 2 cut(s) 21, 23
BstKTI GATC 2 cut(s) 60, 72
BstMBI GATC 2 cut(s) 57, 69
BstMWI GCNNNNNNNGC 3 cut(s) 188, 264, 273
BstNSI RCATGY 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 76
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 175, 242
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 3 cut(s) 17, 21, 138
CfoI GCGC 2 cut(s) 21, 23
Csp6I GTAC 1 cut(s) 175
CviAII CATG 3 cut(s) 5, 104, 178
CviJI RGCY 7 cut(s) 81, 100, 136, 182, 188, 216, 276
CviKI_1 RGCY 7 cut(s) 81, 100, 136, 182, 188, 216, 276
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 3 cut(s) 24, 54, 66
DpnI GATC 2 cut(s) 59, 71
DpnII GATC 2 cut(s) 57, 69
Eco130I CCWWGG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 8, 107, 181
FaiI YATR 5 cut(s) 6, 33, 105, 179, 246
FatI CATG 3 cut(s) 4, 103, 177
Fnu4HI GCNGC 3 cut(s) 189, 256, 274
Fsp4HI GCNGC 3 cut(s) 189, 256, 274
FspBI CTAG 1 cut(s) 183
GlaI GCGC 2 cut(s) 20, 22
GluI GCNGC 3 cut(s) 189, 256, 274
HhaI GCGC 2 cut(s) 21, 23
Hin1II CATG 3 cut(s) 8, 107, 181
Hin6I GCGC 2 cut(s) 19, 21
HinP1I GCGC 2 cut(s) 19, 21
HinfI GANTC 1 cut(s) 44
Hpy188III TCNNGA 1 cut(s) 26
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4V TGCA 2 cut(s) 4, 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 264, 273
HpyF3I CTNAG 3 cut(s) 24, 54, 66
Hsp92II CATG 3 cut(s) 8, 107, 181
HspAI GCGC 2 cut(s) 19, 21
Kzo9I GATC 2 cut(s) 57, 69
LmnI GCTCC 1 cut(s) 105
LpnPI CCDG 4 cut(s) 11, 78, 122, 138
Lsp1109I GCAGC 2 cut(s) 175, 242
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 59, 71
MboI GATC 2 cut(s) 57, 69
MboII GAAGA 2 cut(s) 126, 214
MflI RGATCY 1 cut(s) 69
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 7 cut(s) 21, 121, 126, 154, 161, 202, 206
Mph1103I ATGCAT 1 cut(s) 6
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 188, 264, 273
NdeII GATC 2 cut(s) 57, 69
NlaIII CATG 3 cut(s) 8, 107, 181
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 217
PauI GCGCGC 1 cut(s) 19
PkrI GCNGC 3 cut(s) 190, 257, 275
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PspXI VCTCGAGB 1 cut(s) 217
PsuI RGATCY 1 cut(s) 69
PteI GCGCGC 1 cut(s) 19
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SatI GCNGC 3 cut(s) 189, 256, 274
Sau3AI GATC 2 cut(s) 57, 69
SchI GAGTC 1 cut(s) 38
SetI ASST 4 cut(s) 83, 102, 190, 278
SfcI CTRYAG 1 cut(s) 76
Sfr274I CTCGAG 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 217
SmlI CTYRAG 1 cut(s) 217
SmoI CTYRAG 1 cut(s) 217
SsiI CCGC 1 cut(s) 73
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 89
TaqI TCGA 2 cut(s) 60, 218
TatI WGTACW 1 cut(s) 174
TscAI CASTG 1 cut(s) 92
TseI GCWGC 3 cut(s) 188, 255, 273
TspRI CASTG 1 cut(s) 92
XceI RCATGY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 217
XspI CTAG 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.