Rroxscaffold_3G00243330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001799
Physical Location & Seq
Forward (+)
35867893 .. 35870160
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243330.1

Sequence Viewer

Length: 417 bp
ATGTCGAAGGCGAAGAAGGAGCTGATGGCGTCAGCGCCGTGGAGAGTTCAAGAAGAGGAGGAAGAATTCAGCGACGCGAAGCTGAAAGTGACACGGCAGCAGGCGGGGGCGGAATCAACCATGCACGTCCCTCGCAAGAAAACCAAGCGCGCTCAGGACCATCAAGACGACGACTCTCTCACTGAGATCGACCCTGAGCTCCGCTACAGCTTCCACCGTAACTATCAGTTCCTTCAACGAGTTTTTAGCATTGATACTATCGTGAAACCTCTTCCACCTTCCATGGCTTACAATGTCTCCCGCAACTTGAACTTCTTCACTCGCATTTTCACCCAGTTTTTTGATCCTGAAGGTATAGCAAATGCGCAGAAATCACTCGGTATAGGACAGGAAGAGAAAGCCCGTCGCGTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

16.11

Weight (kDa)

9.22

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 366
AccII CGCG 3 cut(s) 77, 150, 408
AciI CCGC 4 cut(s) 104, 110, 202, 301
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 29
AgsI TTSAA 3 cut(s) 50, 236, 310
AjiI CACGTC 1 cut(s) 127
AluBI AGCT 4 cut(s) 22, 82, 199, 210
AluI AGCT 4 cut(s) 22, 82, 199, 210
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 338
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 1 cut(s) 65
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 4 cut(s) 37, 150, 152, 367
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 109
BccI CCATC 2 cut(s) 19, 168
BceAI ACGGC 2 cut(s) 22, 110
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BcoDI GTCTC 1 cut(s) 301
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 157
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 157
BmrI ACTGGG 1 cut(s) 328
BmuI ACTGGG 1 cut(s) 328
Bpu10I CCTNAGC 2 cut(s) 153, 195
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 38, 282
BsaWI WCCGGW 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bse1I ACTGG 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 38, 282
BseMII CTCAG 3 cut(s) 167, 174, 186
BseNI ACTGG 1 cut(s) 334
BsePI GCGCGC 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 3 cut(s) 77, 150, 408
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 412
BslFI GGGAC 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 186, 343
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 4 cut(s) 104, 110, 202, 301
BspCNI CTCAG 3 cut(s) 166, 175, 187
BspFNI CGCG 3 cut(s) 77, 150, 408
BspPI GGATC 1 cut(s) 338
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 2 cut(s) 38, 282
BssHII GCGCGC 1 cut(s) 148
BssMI GATC 2 cut(s) 186, 343
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 218
Bst6I CTCTTC 3 cut(s) 48, 276, 387
BstACI GRCGYC 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 102, 150
BstDEI CTNAG 3 cut(s) 153, 183, 195
BstDSI CCRYGG 2 cut(s) 38, 282
BstFNI CGCG 3 cut(s) 77, 150, 408
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 4 cut(s) 37, 150, 152, 367
BstKTI GATC 2 cut(s) 189, 346
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 186, 343
BstSFI CTRYAG 1 cut(s) 205
BstUI CGCG 3 cut(s) 77, 150, 408
BstV1I GCAGC 1 cut(s) 109
BtgI CCRYGG 2 cut(s) 38, 282
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 102, 150
CfoI GCGC 4 cut(s) 37, 150, 152, 367
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 3 cut(s) 18, 83, 397
CviAII CATG 2 cut(s) 121, 283
CviJI RGCY 6 cut(s) 22, 82, 199, 210, 287, 401
CviKI_1 RGCY 6 cut(s) 22, 82, 199, 210, 287, 401
DdeI CTNAG 3 cut(s) 153, 183, 195
DpnI GATC 2 cut(s) 188, 345
DpnII GATC 2 cut(s) 186, 343
Eam1104I CTCTTC 3 cut(s) 48, 276, 387
EarI CTCTTC 3 cut(s) 48, 276, 387
EciI GGCGGA 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 124, 286
FaiI YATR 4 cut(s) 122, 284, 356, 383
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 120, 282
FauI CCCGC 2 cut(s) 97, 308
Fnu4HI GCNGC 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 98
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 4 cut(s) 36, 149, 151, 366
GluI GCNGC 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 38
HapII CCGG 1 cut(s) 412
HgaI GACGC 3 cut(s) 18, 83, 397
HhaI GCGC 4 cut(s) 37, 150, 152, 367
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 124, 286
Hin6I GCGC 4 cut(s) 35, 148, 150, 365
HinP1I GCGC 4 cut(s) 35, 148, 150, 365
HinfI GANTC 2 cut(s) 113, 173
HpaII CCGG 1 cut(s) 412
HphI GGTGA 1 cut(s) 322
Hpy188III TCNNGA 5 cut(s) 50, 155, 164, 262, 347
Hpy99I CGWCG 3 cut(s) 77, 173, 408
HpyAV CCTTC 4 cut(s) 10, 242, 288, 344
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 124
HpyF3I CTNAG 3 cut(s) 153, 183, 195
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 124, 286
HspAI GCGC 4 cut(s) 35, 148, 150, 365
Kzo9I GATC 2 cut(s) 186, 343
LmnI GCTCC 2 cut(s) 19, 204
LpnPI CCDG 6 cut(s) 86, 140, 207, 347, 360, 374
Lsp1109I GCAGC 1 cut(s) 109
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 88, 218
MalI GATC 2 cut(s) 188, 345
MboI GATC 2 cut(s) 186, 343
MboII GAAGA 6 cut(s) 25, 65, 74, 263, 307, 404
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 1 cut(s) 65
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 4 cut(s) 49, 52, 141, 279
MroXI GAANNNNTTC 1 cut(s) 314
MspI CCGG 1 cut(s) 412
MvnI CGCG 3 cut(s) 77, 150, 408
NcoI CCATGG 1 cut(s) 282
NdeII GATC 2 cut(s) 186, 343
NlaIII CATG 2 cut(s) 124, 286
NmuCI GTSAC 1 cut(s) 88
NsbI TGCGCA 1 cut(s) 366
PauI GCGCGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
Psp124BI GAGCTC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 157
PteI GCGCGC 1 cut(s) 148
SacI GAGCTC 1 cut(s) 201
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 186, 343
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 167
SduI GDGCHC 1 cut(s) 201
SetI ASST 8 cut(s) 24, 84, 129, 201, 212, 271, 280, 355
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 157
Sse9I AATT 1 cut(s) 65
SsiI CCGC 4 cut(s) 104, 110, 202, 301
SstI GAGCTC 1 cut(s) 201
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 5, 189
TasI AATT 1 cut(s) 65
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 88
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 65
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.