FvH4_6g18060

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002063
Physical Location & Seq
Reverse (-)
11854226 .. 11856144
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18060.t1

Sequence Viewer

Length: 1347 bp
ATGGACACCACTTCACGTCAAGAAGAACAAAACCATGCCACCGTCTCCAAAATGAACAACGATCGTTTAGCTTCCTTGTACCAGAAGATAGCAGAGCCTCCGAGACTTCTCAGCAACGCCGCCGCTAAAGCCTTCTGTTGCATCTTCCGAGTCCCGCAGAGCCTCCTCGAGATAAACGGCAAGTCATACCAACCCCACATCGTCTCCATCGGCCCCTTCCACCACGGAGAGCCCCACCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGTTCTCTGCTTTCAAGAACAGAGCCTAAAGGGTTGACTCTCCAGCACTACTTGAAGTCCATAGAGCCAATGGAAGCTAGAGCCAGAGAGTGTTACTCTGAGACAATCCACCTTAGCTCTGATGAGTTCGTCGAGATGATGGTCCTTGATGGTTGCTTCTTGATCGAGTTGTTTCGAAAAAATGGCCGAGTGGTTCGGTTTGAGCGTGATGATCCACTCGTCAATATGTTCTGGGTAATTCCGTTTATGGTTAGAGATTTTCTTCGTCTTGAAAACCAAATCCCCTACTTTATTCTCCAACATTTGTATGACCTGATTACTACGTCGGAAGATCGTCAAGAATCTGTGAAGTCTTTGTCCTTGCTTGCTTTGGATTTCTTCAACAACCATATGATGAGGCCGCCAGAAGCCATTGCAAAGCTGCATAATCTCACAGGTAAGCATTTGCTTGACTTGGTCCGGTCAAGTGTGATACCAGCAGACCTTAGTGAGCCAGTAAGCAAGCATGATACCCCAACACACCTTATACACTGTGTTTCAAAGCTTCGTCGCGCTGGAATCAAGCTCAATAATCCTCGCAAGGCATTCGAAAGCTTCTTGGTGATCAAGTTCAAGCGTGGAGTTATCGAAATGCCCCAAATCACCATAGACGATTTCATGAGCTCGTTCTTGTTGAACTGTGTGGCCTATGAGCAATGTCACAAGTCATGCTCCAAGCACATCACTACTTACGCTACGTTTCTGGATTACATCGTCAACACTTACAAGGATGTGGAGTATTTGGCTGACAGAAACATAATCGAGAATTGCTTCGGAAACGATGAGGAGGTTGCTAATTTTATTAACAATTTGGGCAAGGATGTGGTTTTTGATCCGGAAAAGTGTTATCTGTCCAAATTGTTCAATGATGTTCATGTGTATTACGGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTACACTTACTTCGACACTCCATGGTCTTTCATTTCGGCTTTTGCTGCTTTGATTTTGCTCATTCTCACCTTGCTGCAGACCTTATACTCTGTTCTCAGTTATTATCATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

449

Amino Acids

52.13

Weight (kDa)

6.47

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 48 - 433 9.4e-126 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 825
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 4 cut(s) 120, 123, 155, 674
AclWI GGATC 2 cut(s) 479, 1139
AcoI YGGCCR 1 cut(s) 457
AfaI GTAC 2 cut(s) 80, 1235
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 9 cut(s) 71, 350, 390, 694, 817, 838, 867, 936, 1227
AluI AGCT 9 cut(s) 71, 350, 390, 694, 817, 838, 867, 936, 1227
Alw21I GWGCWC 2 cut(s) 258, 938
Alw26I GTCTC 4 cut(s) 49, 97, 208, 368
AlwI GGATC 2 cut(s) 479, 1139
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 1147
AoxI GGCC 4 cut(s) 211, 457, 671, 957
ApeKI GCWGC 3 cut(s) 694, 1277, 1306
Asp700I GAANNNNTTC 1 cut(s) 1082
AspLEI GCGC 2 cut(s) 267, 827
AspS9I GGNCC 3 cut(s) 212, 415, 730
AsuHPI GGTGA 3 cut(s) 886, 907, 1291
AsuII TTCGAA 2 cut(s) 448, 861
AsuNHI GCTAGC 1 cut(s) 1223
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 415, 730
BanII GRGCYC 2 cut(s) 234, 938
BarI GAAGNNNNNNTAC 2 cut(s) 1226, 1258
Bbv12I GWGCWC 2 cut(s) 258, 938
BbvI GCAGC 3 cut(s) 681, 1264, 1293
BccI CCATC 3 cut(s) 215, 406, 416
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 841, 875
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 876
BcoDI GTCTC 4 cut(s) 49, 97, 208, 368
BfaI CTAG 2 cut(s) 351, 1224
BfmI CTRYAG 1 cut(s) 1307
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 6 cut(s) 120, 123, 674, 695, 1278, 1307
BlsI GCNGC 6 cut(s) 121, 124, 675, 696, 1279, 1308
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 415, 730
BmeT110I CYCGRG 1 cut(s) 167
BmgBI CACGTC 1 cut(s) 17
BmgT120I GGNCC 3 cut(s) 212, 415, 730
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 150
BmtI GCTAGC 1 cut(s) 1227
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 386
Bpu14I TTCGAA 2 cut(s) 448, 861
BsaJI CCNNGG 3 cut(s) 223, 271, 1253
BsaWI WCCGGW 2 cut(s) 732, 1147
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 245, 275
Bse1I ACTGG 1 cut(s) 767
Bse3DI GCAATG 2 cut(s) 684, 974
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 3 cut(s) 223, 271, 1253
BseGI GGATG 3 cut(s) 1048, 1138, 1339
BseMI GCAATG 2 cut(s) 684, 974
BseMII CTCAG 4 cut(s) 124, 253, 363, 1342
BseNI ACTGG 1 cut(s) 767
BseRI GAGGAG 3 cut(s) 155, 266, 1112
BseXI GCAGC 3 cut(s) 681, 1264, 1293
Bsh1236I CGCG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 213, 459, 673, 959
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 2 cut(s) 258, 938
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 2 cut(s) 733, 1148
BslFI GGGAC 1 cut(s) 137
BsmAI GTCTC 4 cut(s) 49, 97, 208, 368
BsmBI CGTCTC 2 cut(s) 49, 208
BsmFI GGGAC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 4 cut(s) 213, 459, 673, 959
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 2 cut(s) 448, 861
Bsp1286I GDGCHC 3 cut(s) 234, 258, 938
Bsp13I TCCGGA 1 cut(s) 1147
Bsp143I GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
Bsp19I CCATGG 1 cut(s) 1253
BspACI CCGC 4 cut(s) 120, 123, 155, 674
BspANI GGCC 4 cut(s) 213, 459, 673, 959
BspCNI CTCAG 4 cut(s) 123, 252, 364, 1341
BspEI TCCGGA 1 cut(s) 1147
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 1 cut(s) 214
BspMAI CTGCAG 1 cut(s) 1311
BspOI GCTAGC 1 cut(s) 1227
BspPI GGATC 2 cut(s) 479, 1139
BspT104I TTCGAA 2 cut(s) 448, 861
BsrDI GCAATG 2 cut(s) 684, 974
BsrI ACTGG 1 cut(s) 767
BssECI CCNNGG 3 cut(s) 223, 271, 1253
BssMI GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
BssT1I CCWWGG 1 cut(s) 1253
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 43, 806, 953
BstBI TTCGAA 2 cut(s) 448, 861
BstC8I GCNNGC 3 cut(s) 639, 776, 1225
BstDEI CTNAG 6 cut(s) 110, 239, 372, 386, 758, 1328
BstDSI CCRYGG 2 cut(s) 223, 1253
BstF5I GGATG 3 cut(s) 1048, 1138, 1339
BstFNI CGCG 1 cut(s) 825
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 267, 827
BstKTI GATC 7 cut(s) 64, 249, 438, 487, 607, 879, 1147
BstMAI GTCTC 4 cut(s) 49, 97, 208, 368
BstMBI GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 3 cut(s) 128, 262, 1277
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 1214
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 1307
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 3 cut(s) 681, 1264, 1293
BsuRI GGCC 4 cut(s) 213, 459, 673, 959
BtgI CCRYGG 2 cut(s) 223, 1253
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 1048, 1138, 1339
BtsIMutI CAGTG 1 cut(s) 802
Cac8I GCNNGC 3 cut(s) 639, 776, 1225
CciI TCATGA 1 cut(s) 930
CfoI GCGC 2 cut(s) 267, 827
Cfr13I GGNCC 3 cut(s) 212, 415, 730
Csp6I GTAC 2 cut(s) 79, 1234
CviAII CATG 7 cut(s) 35, 779, 931, 981, 1187, 1211, 1254
CviQI GTAC 2 cut(s) 79, 1234
DdeI CTNAG 6 cut(s) 110, 239, 372, 386, 758, 1328
DpnI GATC 7 cut(s) 63, 248, 437, 486, 606, 878, 1146
DpnII GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
EaeI YGGCCR 1 cut(s) 457
Ecl136II GAGCTC 1 cut(s) 936
Eco130I CCWWGG 1 cut(s) 1253
Eco24I GRGCYC 2 cut(s) 234, 938
Eco47I GGWCC 2 cut(s) 415, 730
Eco53kI GAGCTC 1 cut(s) 936
Eco88I CYCGRG 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 936
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 1253
EcoT38I GRGCYC 2 cut(s) 234, 938
ErhI CCWWGG 1 cut(s) 1253
Esp3I CGTCTC 2 cut(s) 49, 208
FaeI CATG 7 cut(s) 38, 782, 934, 984, 1190, 1214, 1257
FaqI GGGAC 1 cut(s) 137
FatI CATG 7 cut(s) 34, 778, 930, 980, 1186, 1210, 1253
FauI CCCGC 1 cut(s) 162
FauNDI CATATG 1 cut(s) 663
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 6 cut(s) 120, 123, 674, 695, 1278, 1307
FokI GGATG 3 cut(s) 1055, 1145, 1326
FriOI GRGCYC 2 cut(s) 234, 938
Fsp4HI GCNGC 6 cut(s) 120, 123, 674, 695, 1278, 1307
FspBI CTAG 2 cut(s) 351, 1224
GlaI GCGC 2 cut(s) 266, 826
GluI GCNGC 6 cut(s) 120, 123, 674, 695, 1278, 1307
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 4 cut(s) 213, 459, 673, 959
HapII CCGG 2 cut(s) 733, 1148
HhaI GCGC 2 cut(s) 267, 827
Hin1II CATG 7 cut(s) 38, 782, 934, 984, 1190, 1214, 1257
Hin6I GCGC 2 cut(s) 265, 825
HinP1I GCGC 2 cut(s) 265, 825
HincII GTYRAC 2 cut(s) 309, 1030
HindII GTYRAC 2 cut(s) 309, 1030
HindIII AAGCTT 2 cut(s) 815, 865
HinfI GANTC 4 cut(s) 150, 310, 614, 831
HpaII CCGG 2 cut(s) 733, 1148
HphI GGTGA 3 cut(s) 886, 907, 1291
Hpy166II GTNNAC 3 cut(s) 309, 1030, 1236
Hpy188I TCNGA 7 cut(s) 102, 149, 242, 373, 394, 601, 1088
Hpy8I GTNNAC 3 cut(s) 309, 1030, 1236
Hpy99I CGWCG 3 cut(s) 407, 601, 825
HpyAV CCTTC 2 cut(s) 142, 226
HpyCH4III ACNGT 3 cut(s) 43, 806, 953
HpyCH4IV ACGT 3 cut(s) 16, 596, 1010
HpyCH4V TGCA 4 cut(s) 141, 689, 697, 1309
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 262, 1277
HpyF3I CTNAG 6 cut(s) 110, 239, 372, 386, 758, 1328
HpySE526I ACGT 3 cut(s) 16, 596, 1010
Hsp92II CATG 7 cut(s) 38, 782, 934, 984, 1190, 1214, 1257
HspAI GCGC 2 cut(s) 265, 825
Kpn2I TCCGGA 1 cut(s) 1147
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
LmnI GCTCC 2 cut(s) 253, 989
Lsp1109I GCAGC 3 cut(s) 681, 1264, 1293
LweI GCATC 1 cut(s) 150
MaeI CTAG 2 cut(s) 351, 1224
MaeII ACGT 3 cut(s) 16, 596, 1010
MaeIII GTNAC 2 cut(s) 365, 971
MalI GATC 7 cut(s) 63, 248, 437, 486, 606, 878, 1146
MboI GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
MboII GAAGA 6 cut(s) 35, 97, 136, 527, 614, 643
MhlI GDGCHC 3 cut(s) 234, 258, 938
MluCI AATT 5 cut(s) 510, 1078, 1108, 1120, 1169
MlyI GAGTC 2 cut(s) 159, 304
MmeI TCCRAC 2 cut(s) 579, 595
MnlI CCTC 9 cut(s) 108, 173, 176, 244, 248, 663, 858, 1090, 1093
MroI TCCGGA 1 cut(s) 1147
MroXI GAANNNNTTC 1 cut(s) 1082
MseI TTAA 1 cut(s) 1116
MslI CAYNNNNRTG 1 cut(s) 579
MspI CCGG 2 cut(s) 733, 1148
MspR9I CCNGG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 3 cut(s) 128, 262, 1277
NcoI CCATGG 1 cut(s) 1253
NdeI CATATG 1 cut(s) 663
NdeII GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
NheI GCTAGC 1 cut(s) 1223
NlaIII CATG 7 cut(s) 38, 782, 934, 984, 1190, 1214, 1257
NlaIV GGNNCC 1 cut(s) 214
NmeAIII GCCGAG 1 cut(s) 485
NmuCI GTSAC 1 cut(s) 971
NspI RCATGY 1 cut(s) 1214
NspV TTCGAA 2 cut(s) 448, 861
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 1 cut(s) 930
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PctI GAATGC 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 2 cut(s) 614, 831
PflFI GACNNNGTC 1 cut(s) 728
PkrI GCNGC 6 cut(s) 121, 124, 675, 696, 1279, 1308
Ple19I CGATCG 1 cut(s) 64
PleI GAGTC 2 cut(s) 158, 304
PpsI GAGTC 2 cut(s) 158, 304
Psp124BI GAGCTC 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 3 cut(s) 212, 415, 730
PstI CTGCAG 1 cut(s) 1311
PsyI GACNNNGTC 1 cut(s) 728
PvuI CGATCG 1 cut(s) 64
RsaI GTAC 2 cut(s) 80, 1235
RsaNI GTAC 2 cut(s) 79, 1234
RseI CAYNNNNRTG 1 cut(s) 579
SacI GAGCTC 1 cut(s) 938
SaqAI TTAA 1 cut(s) 1116
SatI GCNGC 6 cut(s) 120, 123, 674, 695, 1278, 1307
Sau3AI GATC 7 cut(s) 61, 246, 435, 484, 604, 876, 1144
Sau96I GGNCC 3 cut(s) 212, 415, 730
SchI GAGTC 2 cut(s) 159, 304
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 234, 258, 938
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 1307
Sfr274I CTCGAG 1 cut(s) 167
SfuI TTCGAA 2 cut(s) 448, 861
SinI GGWCC 2 cut(s) 415, 730
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 5 cut(s) 510, 1078, 1108, 1120, 1169
SsiI CCGC 4 cut(s) 120, 123, 155, 674
SspMI CTAG 2 cut(s) 351, 1224
SstI GAGCTC 1 cut(s) 938
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 1253
TaaI ACNGT 3 cut(s) 43, 806, 953
TaiI ACGT 3 cut(s) 19, 599, 1013
TaqI TCGA 9 cut(s) 168, 249, 405, 438, 448, 861, 900, 1074, 1245
TasI AATT 5 cut(s) 510, 1078, 1108, 1120, 1169
TatI WGTACW 1 cut(s) 1233
TauI GCSGC 3 cut(s) 122, 125, 676
TfiI GAWTC 2 cut(s) 614, 831
Tru1I TTAA 1 cut(s) 1116
Tru9I TTAA 1 cut(s) 1116
TscAI CASTG 1 cut(s) 809
TseFI GTSAC 1 cut(s) 971
TseI GCWGC 3 cut(s) 694, 1277, 1306
Tsp45I GTSAC 1 cut(s) 971
TspDTI ATGAA 4 cut(s) 68, 919, 1175, 1252
TspGWI ACGGA 2 cut(s) 240, 504
TspRI CASTG 1 cut(s) 809
Tth111I GACNNNGTC 1 cut(s) 728
VpaK11BI GGWCC 2 cut(s) 415, 730
XceI RCATGY 1 cut(s) 1214
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 2 cut(s) 351, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.