Rroxscaffold_6G00409360

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002063
Physical Location & Seq
Reverse (-)
31876883 .. 31878867
1985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409360.1

Sequence Viewer

Length: 1335 bp
ATGGATACCAATCCAGTTCAAGAAGAACACAACCATGTCACCCCCATCTCCGGAATGAACAATGATCGCCTAGCTTCCATGTTCCAGAAGATCGCAGACCCTCCACAACTCCTCAGCAACGCTGCCGGCAAAGCCTCCTGTTGCATCTTCCGAGTCCCGCAGAACCTTGTCGAGATAAACGGCAAGTCGTACCAGCCCCAAATCGTCTCCATCGGTCCCTTCCACCGCGGCGAGCCCCACCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGCTCTCTGCTTTCAAGAACAGAGCTCAAAGGGTTGACTCTCCAGCACTACTTGAAGTCCATAGAGCCAATGGAAGCTAGAGCCAGAGAGTGTTACTCTGAAACAATCCACCTTAGCAGTGATGAGTTCGTCGAAATGATGGTTGTTGATGGTTGCTTCTTAATCGAGATGTTTCGAAAATATGGCCGTGTGGTTCGGTTTGAGCGCGATGATCCACTCATCAATATGTTCTGGATACTTCCGATTATGGTTAGAGATTTTCTTCGTCTTGAGAACCAAATCCCCTACTTTATACTTGAGCATTTGTATGATCTAATAACCAAGTCAGAAGATCGTCAAGACTCTGTGAAGTCTTTGTCCTTGCTTGCTTTGGACTTCTTCAACAACCATATGATGAGACCGCAAGAAGTCATTGCAAAGCTGCATAATCTTTCAGGTACGCATTTGCTTGACTTACTCCGGTCAAGTGTAATACCAGCCGACCTTGTTGAACCAGGACGCACCCCAACCAACATTATACGTTGTGTTTCAACGCTTCGTCGCGCTGGAATCAAGCTCAATCCTACTAGAGAATACGAAAGCTTCTTGGAGATCAAGTTCAAGCGTGGAGTTATAGAAATGCCTCCAATCACCATTGACGATTTCATGAGCTCATTCTTGTTGAACTGTGTGGCCTATGAGCAATGCCACCAGTCATCCTCTAAACACATCTCCACTTACACATTCTGGCTGGATTGCCTAGTGAATACTTACAAGGATGTCGAGTATTTGGCTGACATAAACATAATCGAGAATTGGTTCGGAAGCGATGAAGAGGTTGCTCATTTTATTAACAATTTGGGGAAGGATGTGGCTTTTGATATTGATAGATGTCATCTGTCGAAATTGTTCAATGATCTACATCTGTATTACAGGAATAGTTGGCATGTTCAATGGGCAAGCTTCAAGTACATTTACTTTCCGGATCCATGGTCGTTTCTTTCGGCTTTTGCTGTGTTGATTTTCTTTTTTACCTTGCTGCAGACCTTATACACTGTTTTAAGTTATTATCATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

444

Amino Acids

51.94

Weight (kDa)

5.91

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 428 4.5e-119 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 228, 483, 819
AccIII TCCGGA 2 cut(s) 50, 1237
AciI CCGC 4 cut(s) 158, 226, 228, 677
AclWI GGATC 3 cut(s) 482, 1235, 1248
AcoI YGGCCR 1 cut(s) 460
AfaI GTAC 3 cut(s) 191, 715, 1226
AfiI CCNNNNNNNGG 1 cut(s) 50
AjnI CCWGG 2 cut(s) 273, 769
AloI GAACNNNNNNTCC 2 cut(s) 857, 889
AluBI AGCT 8 cut(s) 74, 301, 353, 697, 832, 858, 927, 1218
AluI AGCT 8 cut(s) 74, 301, 353, 697, 832, 858, 927, 1218
Alw21I GWGCWC 3 cut(s) 261, 303, 929
Alw26I GTCTC 2 cut(s) 211, 667
AlwI GGATC 3 cut(s) 482, 1235, 1248
Aor13HI TCCGGA 2 cut(s) 50, 1237
AoxI GGCC 2 cut(s) 460, 948
ApeKI GCWGC 3 cut(s) 122, 697, 1294
Asp700I GAANNNNTTC 2 cut(s) 1073, 1163
AspLEI GCGC 3 cut(s) 270, 483, 821
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 31, 898
AsuII TTCGAA 1 cut(s) 451
AvaII GGWCC 1 cut(s) 215
BamHI GGATCC 1 cut(s) 1240
BanII GRGCYC 4 cut(s) 237, 281, 303, 929
Bbv12I GWGCWC 3 cut(s) 261, 303, 929
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 109, 684, 1281
BccI CCATC 4 cut(s) 53, 218, 409, 419
BceAI ACGGC 2 cut(s) 196, 447
BciT130I CCWGG 2 cut(s) 275, 771
BciVI GTATCC 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 211, 667
BfaI CTAG 4 cut(s) 71, 354, 843, 1016
BfmI CTRYAG 1 cut(s) 1295
BfoI RGCGCY 1 cut(s) 271
BfuI GTATCC 1 cut(s) 504
BisI GCNGC 4 cut(s) 123, 229, 698, 1295
BlsI GCNGC 4 cut(s) 124, 230, 699, 1296
Bme1390I CCNGG 2 cut(s) 275, 771
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 217, 1242
BmrFI CCNGG 2 cut(s) 275, 771
BmsI GCATC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 302
Bpu10I CCTNAGC 2 cut(s) 113, 389
Bpu14I TTCGAA 1 cut(s) 451
BpuEI CTTGAG 2 cut(s) 566, 593
BsaBI GATNNNNATC 2 cut(s) 9, 1176
BsaI GGTCTC 1 cut(s) 667
BsaJI CCNNGG 3 cut(s) 226, 274, 1244
BsaWI WCCGGW 3 cut(s) 50, 735, 1237
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 857, 887
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 14, 967
Bse3DI GCAATG 2 cut(s) 687, 965
Bse8I GATNNNNATC 2 cut(s) 9, 1176
BseAI TCCGGA 2 cut(s) 50, 1237
BseBI CCWGG 2 cut(s) 275, 771
BseDI CCNNGG 3 cut(s) 226, 274, 1244
BseGI GGATG 3 cut(s) 971, 1039, 1129
BseJI GATNNNNATC 2 cut(s) 9, 1176
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 2 cut(s) 687, 965
BseMII CTCAG 2 cut(s) 127, 256
BseNI ACTGG 2 cut(s) 14, 967
BseRI GAGGAG 2 cut(s) 101, 269
BseXI GCAGC 3 cut(s) 109, 684, 1281
Bsh1236I CGCG 3 cut(s) 228, 483, 819
BshFI GGCC 2 cut(s) 462, 950
BsiHKAI GWGCWC 3 cut(s) 261, 303, 929
BsiSI CCGG 4 cut(s) 51, 126, 736, 1238
BslFI GGGAC 2 cut(s) 140, 201
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 211, 667
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 140, 201
BsnI GGCC 2 cut(s) 462, 950
Bso31I GGTCTC 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 5 cut(s) 237, 261, 281, 303, 929
Bsp13I TCCGGA 2 cut(s) 50, 1237
Bsp143I GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
Bsp19I CCATGG 1 cut(s) 1244
BspACI CCGC 4 cut(s) 158, 226, 228, 677
BspANI GGCC 2 cut(s) 462, 950
BspCNI CTCAG 2 cut(s) 126, 255
BspEI TCCGGA 2 cut(s) 50, 1237
BspFNI CGCG 3 cut(s) 228, 483, 819
BspHI TCATGA 1 cut(s) 921
BspLI GGNNCC 2 cut(s) 217, 1242
BspMAI CTGCAG 1 cut(s) 1299
BspPI GGATC 3 cut(s) 482, 1235, 1248
BspT104I TTCGAA 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 667
BsrDI GCAATG 2 cut(s) 687, 965
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 14, 967
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 226, 274, 1244
BssMI GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
BssT1I CCWWGG 1 cut(s) 1244
Bst2UI CCWGG 2 cut(s) 275, 771
Bst4CI ACNGT 2 cut(s) 944, 1312
Bst6I CTCTTC 1 cut(s) 1083
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 4 cut(s) 127, 233, 642, 1216
BstDEI CTNAG 3 cut(s) 113, 242, 389
BstDSI CCRYGG 2 cut(s) 226, 1244
BstF5I GGATG 3 cut(s) 971, 1039, 1129
BstFNI CGCG 3 cut(s) 228, 483, 819
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 3 cut(s) 270, 483, 821
BstKTI GATC 9 cut(s) 67, 93, 252, 490, 589, 610, 870, 1174, 1243
BstMAI GTCTC 2 cut(s) 211, 667
BstMBI GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
BstMWI GCNNNNNNNGC 3 cut(s) 131, 265, 276
BstNI CCWGG 2 cut(s) 275, 771
BstNSI RCATGY 1 cut(s) 1205
BstSCI CCNGG 2 cut(s) 273, 769
BstSFI CTRYAG 1 cut(s) 1295
BstUI CGCG 3 cut(s) 228, 483, 819
BstV1I GCAGC 3 cut(s) 109, 684, 1281
BstX2I RGATCY 1 cut(s) 1240
BstYI RGATCY 1 cut(s) 1240
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 2 cut(s) 462, 950
BtgI CCRYGG 2 cut(s) 226, 1244
BtgZI GCGATG 2 cut(s) 498, 1098
BtsCI GGATG 3 cut(s) 971, 1039, 1129
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 400, 1308
Cac8I GCNNGC 4 cut(s) 127, 233, 642, 1216
CciI TCATGA 1 cut(s) 921
CfoI GCGC 3 cut(s) 270, 483, 821
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 215
Cfr42I CCGCGG 1 cut(s) 229
CseI GACGC 1 cut(s) 783
Csp6I GTAC 3 cut(s) 190, 714, 1225
CviAII CATG 5 cut(s) 35, 79, 922, 1202, 1245
CviQI GTAC 3 cut(s) 190, 714, 1225
DdeI CTNAG 3 cut(s) 113, 242, 389
DpnI GATC 9 cut(s) 66, 92, 251, 489, 588, 609, 869, 1173, 1242
DpnII GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
EaeI YGGCCR 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Ecl136II GAGCTC 2 cut(s) 301, 927
Eco130I CCWWGG 1 cut(s) 1244
Eco24I GRGCYC 4 cut(s) 237, 281, 303, 929
Eco31I GGTCTC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 2 cut(s) 301, 927
EcoICRI GAGCTC 2 cut(s) 301, 927
EcoRII CCWGG 2 cut(s) 273, 769
EcoT14I CCWWGG 1 cut(s) 1244
EcoT38I GRGCYC 4 cut(s) 237, 281, 303, 929
ErhI CCWWGG 1 cut(s) 1244
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 5 cut(s) 38, 82, 925, 1205, 1248
FaqI GGGAC 2 cut(s) 140, 201
FatI CATG 5 cut(s) 34, 78, 921, 1201, 1244
FauI CCCGC 1 cut(s) 165
FauNDI CATATG 1 cut(s) 666
Fnu4HI GCNGC 4 cut(s) 123, 229, 698, 1295
FokI GGATG 3 cut(s) 958, 1046, 1136
FriOI GRGCYC 4 cut(s) 237, 281, 303, 929
Fsp4HI GCNGC 4 cut(s) 123, 229, 698, 1295
FspBI CTAG 4 cut(s) 71, 354, 843, 1016
GlaI GCGC 3 cut(s) 269, 482, 820
GluI GCNGC 4 cut(s) 123, 229, 698, 1295
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 2 cut(s) 462, 950
HapII CCGG 4 cut(s) 51, 126, 736, 1238
HgaI GACGC 1 cut(s) 783
HhaI GCGC 3 cut(s) 270, 483, 821
Hin1II CATG 5 cut(s) 38, 82, 925, 1205, 1248
Hin6I GCGC 3 cut(s) 268, 481, 819
HinP1I GCGC 3 cut(s) 268, 481, 819
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 2 cut(s) 856, 1216
HinfI GANTC 4 cut(s) 153, 313, 617, 825
HpaII CCGG 4 cut(s) 51, 126, 736, 1238
HphI GGTGA 2 cut(s) 31, 898
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 6 cut(s) 152, 245, 376, 519, 604, 1079
Hpy8I GTNNAC 1 cut(s) 312
Hpy99I CGWCG 2 cut(s) 410, 819
HpyAV CCTTC 2 cut(s) 229, 1114
HpyCH4III ACNGT 2 cut(s) 944, 1312
HpyCH4IV ACGT 1 cut(s) 796
HpyCH4V TGCA 4 cut(s) 144, 692, 700, 1297
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 265, 276
HpyF3I CTNAG 3 cut(s) 113, 242, 389
HpySE526I ACGT 1 cut(s) 796
Hsp92II CATG 5 cut(s) 38, 82, 925, 1205, 1248
HspAI GCGC 3 cut(s) 268, 481, 819
Kpn2I TCCGGA 2 cut(s) 50, 1237
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 3 cut(s) 109, 684, 1281
LweI GCATC 1 cut(s) 153
MaeI CTAG 4 cut(s) 71, 354, 843, 1016
MaeII ACGT 1 cut(s) 796
MaeIII GTNAC 2 cut(s) 37, 368
MalI GATC 9 cut(s) 66, 92, 251, 489, 588, 609, 869, 1173, 1242
MboI GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
MboII GAAGA 7 cut(s) 35, 100, 139, 530, 617, 646, 1100
MflI RGATCY 1 cut(s) 1240
MhlI GDGCHC 5 cut(s) 237, 261, 281, 303, 929
MluCI AATT 3 cut(s) 1069, 1111, 1160
MlyI GAGTC 3 cut(s) 162, 307, 611
MnlI CCTC 8 cut(s) 111, 122, 145, 247, 251, 909, 985, 1084
MroI TCCGGA 2 cut(s) 50, 1237
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 1073, 1163
MseI TTAA 3 cut(s) 437, 1107, 1316
MslI CAYNNNNRTG 3 cut(s) 33, 500, 582
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 4 cut(s) 51, 126, 736, 1238
MspR9I CCNGG 2 cut(s) 275, 771
MvaI CCWGG 2 cut(s) 275, 771
MvnI CGCG 3 cut(s) 228, 483, 819
MwoI GCNNNNNNNGC 3 cut(s) 131, 265, 276
NaeI GCCGGC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 1244
NdeI CATATG 1 cut(s) 666
NdeII GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 5 cut(s) 38, 82, 925, 1205, 1248
NlaIV GGNNCC 2 cut(s) 217, 1242
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 1205
NspV TTCGAA 1 cut(s) 451
PagI TCATGA 1 cut(s) 921
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 1073, 1163
PfeI GAWTC 1 cut(s) 825
PkrI GCNGC 4 cut(s) 124, 230, 699, 1296
PleI GAGTC 3 cut(s) 161, 307, 611
PpsI GAGTC 3 cut(s) 161, 307, 611
Psp124BI GAGCTC 2 cut(s) 303, 929
Psp6I CCWGG 2 cut(s) 273, 769
PspGI CCWGG 2 cut(s) 273, 769
PspN4I GGNNCC 2 cut(s) 217, 1242
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 1299
PsuI RGATCY 1 cut(s) 1240
RsaI GTAC 3 cut(s) 191, 715, 1226
RsaNI GTAC 3 cut(s) 190, 714, 1225
RseI CAYNNNNRTG 3 cut(s) 33, 500, 582
SacI GAGCTC 2 cut(s) 303, 929
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 3 cut(s) 437, 1107, 1316
SatI GCNGC 4 cut(s) 123, 229, 698, 1295
Sau3AI GATC 9 cut(s) 64, 90, 249, 487, 586, 607, 867, 1171, 1240
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 3 cut(s) 162, 307, 611
ScrFI CCNGG 2 cut(s) 275, 771
SduI GDGCHC 5 cut(s) 237, 261, 281, 303, 929
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 1295
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 451
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 3 cut(s) 33, 500, 582
SmlI CTYRAG 2 cut(s) 545, 572
SmoI CTYRAG 2 cut(s) 545, 572
Sse9I AATT 3 cut(s) 1069, 1111, 1160
SsiI CCGC 4 cut(s) 158, 226, 228, 677
SspMI CTAG 4 cut(s) 71, 354, 843, 1016
SstI GAGCTC 2 cut(s) 303, 929
StyD4I CCNGG 2 cut(s) 273, 769
StyI CCWWGG 1 cut(s) 1244
TaaI ACNGT 2 cut(s) 944, 1312
TaiI ACGT 1 cut(s) 799
TaqI TCGA 8 cut(s) 171, 252, 408, 441, 451, 1038, 1065, 1157
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 1069, 1111, 1160
TatI WGTACW 1 cut(s) 1224
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 825
Tru1I TTAA 3 cut(s) 437, 1107, 1316
Tru9I TTAA 3 cut(s) 437, 1107, 1316
TscAI CASTG 2 cut(s) 400, 1315
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 122, 697, 1294
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 71, 910, 1101
TspRI CASTG 2 cut(s) 400, 1315
VpaK11BI GGWCC 1 cut(s) 215
XceI RCATGY 1 cut(s) 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmnI GAANNNNTTC 2 cut(s) 1073, 1163
XspI CTAG 4 cut(s) 71, 354, 843, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.