pycom12g01590

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002063
Physical Location & Seq
Reverse (-)
1323896 .. 1325799
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01590.1

Sequence Viewer

Length: 930 bp
ATGGACCCCAATCCACAACAGCAACAACCCAACAACTACCACCATCCAATCAGCATCTGGGAAGCGAACAAGGATCGGTTGGGCACCATGTACCAGATGATCTCAGAACCCCCAAGACTCCTAAGCAATGCAGCAGGAAAACCATCCTGCTGCATCTTCAGAGTCCCACAGAGCCTTGTCGACATAAACGGTAAGTCCTACAACCCCCACATCGTCTCAATGGGTCCTTACCACAGAGGAGAGCCACGTCTCAAGATGATCGAAGAGCACAAGTGGAAATACTTGGGTTCTTTGCTTGCAAGGACAGAGCCCAAAGGGTTGACTTTAAAGGACTACTTGAAGGCCTTGGAGCCATTGGAACAAAAAGCTAGAGAGTGTTACTCAGAAAGCATAAATCTTAGCACGGATGAATTTTTGGAAATGTTGGCTCTTGATAGTTGCTTTATAATCGAATTGTTTCGAAAATTTGGGCTTTTGGTTCGGTTCGAGCGTGACGATCCTCTTGTCAACATGGTTTGGATAATCCCATTTTTGGCTAGGGATTTTCTTAGGCTTGAAAATCAAATACCCTACTTTGTTCTTCTGACCTTGTTTGATCTCACAACAATGGAAGAACATAAAGAAAATGGGAAGTCATTGTCGTTGCTTGCGTTGGAATTCTTCAACAACCATATGCAGAGGCCTGATCATGTCATTCAAAAGCACCACAATCTTATAGGTATGCATTTGCTTGACTTGTTACGCTCAAGTGTTATACCACCAGGCCATGAAGAGCCACGTAGCAGCAACACCATCCCAACACATACCATCCACTGTGTCTCGAAGCTTCGCCGTGCCGGAATCAAGCTCAATCCGGGCAAGGACGAAAGCTTCTTGATCGATCAAATTCAAGCGCGGTGTCATCGAAATGCCGCCTATAACAATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

310

Amino Acids

35.85

Weight (kDa)

7.72

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 52 - 291 8.4e-65 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 895
AciI CCGC 2 cut(s) 895, 912
AclWI GGATC 2 cut(s) 81, 491
AcsI RAATTY 4 cut(s) 410, 464, 656, 885
AcuI CTGAAG 1 cut(s) 142
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 532
AgsI TTSAA 5 cut(s) 340, 557, 664, 698, 890
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 1 cut(s) 760
AluBI AGCT 4 cut(s) 368, 826, 847, 870
AluI AGCT 4 cut(s) 368, 826, 847, 870
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 3 cut(s) 220, 254, 823
AlwI GGATC 2 cut(s) 81, 491
AlwNI CAGNNNCTG 1 cut(s) 57
AoxI GGCC 3 cut(s) 342, 680, 763
ApeKI GCWGC 3 cut(s) 131, 150, 783
ApoI RAATTY 4 cut(s) 410, 464, 656, 885
Asp700I GAANNNNTTC 1 cut(s) 456
AspLEI GCGC 1 cut(s) 895
AspS9I GGNCC 2 cut(s) 4, 224
AsuC2I CCSGG 1 cut(s) 855
AsuII TTCGAA 1 cut(s) 460
AvaII GGWCC 2 cut(s) 4, 224
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 137, 143, 795
BccI CCATC 4 cut(s) 51, 151, 800, 815
BceAI ACGGC 1 cut(s) 816
BciT130I CCWGG 1 cut(s) 762
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 220, 254, 823
BfaI CTAG 2 cut(s) 369, 537
BisI GCNGC 4 cut(s) 132, 151, 784, 912
BlsI GCNGC 4 cut(s) 133, 152, 785, 913
Bme1390I CCNGG 2 cut(s) 762, 855
Bme18I GGWCC 2 cut(s) 4, 224
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 4, 224
BmiI GGNNCC 4 cut(s) 6, 85, 225, 351
BmrFI CCNGG 2 cut(s) 762, 855
BmsI GCATC 2 cut(s) 63, 162
BplI GAGNNNNNCTC 2 cut(s) 365, 397
Bpu10I CCTNAGC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 2 cut(s) 236, 730
BpuMI CCSGG 1 cut(s) 855
Bsa29I ATCGAT 2 cut(s) 879, 925
BsaAI YACGTR 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 1 cut(s) 532
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 762
BseCI ATCGAT 2 cut(s) 879, 925
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 5 cut(s) 43, 143, 412, 792, 807
BseLI CCNNNNNNNGG 1 cut(s) 532
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 2 cut(s) 117, 396
BseRI GAGGAG 1 cut(s) 252
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 3 cut(s) 137, 143, 795
Bsh1236I CGCG 1 cut(s) 895
BshFI GGCC 3 cut(s) 344, 682, 765
BshNI GGYRCC 1 cut(s) 83
BshVI ATCGAT 2 cut(s) 879, 925
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 2 cut(s) 837, 854
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 532
BsmAI GTCTC 3 cut(s) 220, 254, 823
BsmBI CGTCTC 2 cut(s) 220, 254
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 3 cut(s) 344, 682, 765
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 3 cut(s) 86, 270, 312
Bsp143I GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
BspACI CCGC 2 cut(s) 895, 912
BspANI GGCC 3 cut(s) 344, 682, 765
BspCNI CTCAG 2 cut(s) 116, 395
BspDI ATCGAT 2 cut(s) 879, 925
BspFNI CGCG 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 6, 85, 225, 351
BspPI GGATC 2 cut(s) 81, 491
BspQI GCTCTTC 2 cut(s) 258, 765
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 762
Bst4CI ACNGT 2 cut(s) 191, 815
Bst6I CTCTTC 2 cut(s) 258, 765
BstBAI YACGTR 1 cut(s) 779
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 297, 648
BstDEI CTNAG 5 cut(s) 103, 122, 382, 398, 548
BstF5I GGATG 5 cut(s) 43, 143, 412, 792, 807
BstFNI CGCG 1 cut(s) 895
BstHHI GCGC 1 cut(s) 895
BstKTI GATC 8 cut(s) 76, 102, 261, 499, 598, 688, 879, 883
BstMAI GTCTC 3 cut(s) 220, 254, 823
BstMBI GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
BstNI CCWGG 1 cut(s) 762
BstSCI CCNGG 2 cut(s) 760, 853
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 895
BstV1I GCAGC 3 cut(s) 137, 143, 795
Bsu15I ATCGAT 2 cut(s) 879, 925
BsuRI GGCC 3 cut(s) 344, 682, 765
BsuTUI ATCGAT 2 cut(s) 879, 925
BtrI CACGTC 1 cut(s) 248
BtsCI GGATG 5 cut(s) 43, 143, 412, 792, 807
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 2 cut(s) 297, 648
CaiI CAGNNNCTG 1 cut(s) 57
CfoI GCGC 1 cut(s) 895
Cfr13I GGNCC 2 cut(s) 4, 224
ClaI ATCGAT 2 cut(s) 879, 925
Csp6I GTAC 1 cut(s) 91
CviAII CATG 4 cut(s) 88, 511, 689, 767
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 5 cut(s) 103, 122, 382, 398, 548
DpnI GATC 8 cut(s) 75, 101, 260, 498, 597, 687, 878, 882
DpnII GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
DraI TTTAAA 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 258, 765
EarI CTCTTC 2 cut(s) 258, 765
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 2 cut(s) 344, 682
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 4, 224
Eco57I CTGAAG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 656
EcoRII CCWGG 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 2 cut(s) 220, 254
FaeI CATG 4 cut(s) 91, 514, 692, 770
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 149
FatI CATG 4 cut(s) 87, 510, 688, 766
FauNDI CATATG 1 cut(s) 672
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 132, 151, 784, 912
FokI GGATG 5 cut(s) 30, 130, 419, 779, 794
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 4 cut(s) 132, 151, 784, 912
FspBI CTAG 2 cut(s) 369, 537
GlaI GCGC 1 cut(s) 894
GluI GCNGC 4 cut(s) 132, 151, 784, 912
HaeIII GGCC 3 cut(s) 344, 682, 765
HapII CCGG 2 cut(s) 837, 854
HhaI GCGC 1 cut(s) 895
Hin1II CATG 4 cut(s) 91, 514, 692, 770
Hin6I GCGC 1 cut(s) 893
HinP1I GCGC 1 cut(s) 893
HincII GTYRAC 3 cut(s) 181, 321, 508
HindII GTYRAC 3 cut(s) 181, 321, 508
HindIII AAGCTT 2 cut(s) 824, 868
HinfI GANTC 3 cut(s) 117, 162, 840
HpaII CCGG 2 cut(s) 837, 854
Hpy166II GTNNAC 3 cut(s) 181, 321, 508
Hpy188I TCNGA 4 cut(s) 106, 161, 385, 585
Hpy188III TCNNGA 4 cut(s) 253, 431, 820, 874
Hpy8I GTNNAC 3 cut(s) 181, 321, 508
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 2 cut(s) 191, 815
HpyCH4IV ACGT 2 cut(s) 247, 778
HpyCH4V TGCA 5 cut(s) 131, 153, 299, 676, 724
HpyF3I CTNAG 5 cut(s) 103, 122, 382, 398, 548
HpySE526I ACGT 2 cut(s) 247, 778
Hsp92II CATG 4 cut(s) 91, 514, 692, 770
HspAI GCGC 1 cut(s) 893
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
LguI GCTCTTC 2 cut(s) 258, 765
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 9 cut(s) 43, 107, 120, 160, 696, 747, 774, 850, 867
Lsp1109I GCAGC 3 cut(s) 137, 143, 795
LweI GCATC 2 cut(s) 63, 162
MaeI CTAG 2 cut(s) 369, 537
MaeII ACGT 2 cut(s) 247, 778
MaeIII GTNAC 3 cut(s) 377, 491, 738
MalI GATC 8 cut(s) 75, 101, 260, 498, 597, 687, 878, 882
MboI GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
MboII GAAGA 6 cut(s) 148, 275, 572, 623, 652, 782
MhlI GDGCHC 3 cut(s) 86, 270, 312
MluCI AATT 5 cut(s) 410, 452, 464, 656, 885
MlyI GAGTC 2 cut(s) 111, 171
MmeI TCCRAC 1 cut(s) 633
MnlI CCTC 3 cut(s) 230, 510, 672
Mph1103I ATGCAT 1 cut(s) 726
MroXI GAANNNNTTC 1 cut(s) 456
MseI TTAA 1 cut(s) 326
MslI CAYNNNNRTG 2 cut(s) 605, 906
MspI CCGG 2 cut(s) 837, 854
MspR9I CCNGG 2 cut(s) 762, 855
MvaI CCWGG 1 cut(s) 762
MvnI CGCG 1 cut(s) 895
NciI CCSGG 1 cut(s) 855
NdeI CATATG 1 cut(s) 672
NdeII GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
NlaIII CATG 4 cut(s) 91, 514, 692, 770
NlaIV GGNNCC 4 cut(s) 6, 85, 225, 351
NmuCI GTSAC 1 cut(s) 491
NsiI ATGCAT 1 cut(s) 726
NspV TTCGAA 1 cut(s) 460
PceI AGGCCT 2 cut(s) 344, 682
PciSI GCTCTTC 2 cut(s) 258, 765
PcsI WCGNNNNNNNCGW 3 cut(s) 487, 492, 647
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 840
PkrI GCNGC 4 cut(s) 133, 152, 785, 913
PleI GAGTC 2 cut(s) 111, 170
PpsI GAGTC 2 cut(s) 111, 170
Ppu21I YACGTR 1 cut(s) 779
PpuMI RGGWCCY 1 cut(s) 224
PsiI TTATAA 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 760
PspGI CCWGG 1 cut(s) 760
PspN4I GGNNCC 4 cut(s) 6, 85, 225, 351
PspPI GGNCC 2 cut(s) 4, 224
PspPPI RGGWCCY 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 57
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 605, 906
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 2 cut(s) 258, 765
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 4 cut(s) 132, 151, 784, 912
Sau3AI GATC 8 cut(s) 73, 99, 258, 496, 595, 685, 876, 880
Sau96I GGNCC 2 cut(s) 4, 224
SchI GAGTC 2 cut(s) 111, 171
ScrFI CCNGG 2 cut(s) 762, 855
SduI GDGCHC 3 cut(s) 86, 270, 312
SetI ASST 8 cut(s) 250, 370, 590, 721, 781, 828, 849, 872
SfaNI GCATC 2 cut(s) 63, 162
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 2 cut(s) 4, 224
SmiMI CAYNNNNRTG 2 cut(s) 605, 906
SmlI CTYRAG 2 cut(s) 251, 745
SmoI CTYRAG 2 cut(s) 251, 745
Sse9I AATT 5 cut(s) 410, 452, 464, 656, 885
SseBI AGGCCT 2 cut(s) 344, 682
SsiI CCGC 2 cut(s) 895, 912
SspMI CTAG 2 cut(s) 369, 537
StuI AGGCCT 2 cut(s) 344, 682
StyD4I CCNGG 2 cut(s) 760, 853
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 191, 815
TaiI ACGT 2 cut(s) 250, 781
TaqI TCGA 9 cut(s) 180, 261, 450, 460, 486, 821, 879, 904, 925
TasI AATT 5 cut(s) 410, 452, 464, 656, 885
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 1 cut(s) 840
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 1 cut(s) 818
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 3 cut(s) 131, 150, 783
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 423, 783
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 4, 224
XapI RAATTY 4 cut(s) 410, 464, 656, 885
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 2 cut(s) 369, 537
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.