MD12G1019200.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002063
Physical Location & Seq
Reverse (-)
1889630 .. 1891916
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1019200.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGGCCCGAATTCACCGCAACAACAGCAACAACCCAACAATAACAGCCATCCAATCAGCATGTGGGAAGCGAACAAGGATCGGTTGCTCACCATGTACCAGATGATCTCAGAACCCCCAAGACTTCTAAGCAATGCAGCAGGAAAACCCTCCTGCTGCATCTTCAGAGTCCCACAGAGCCTTGTCGAGATAAACGGTAAGTCCTACAACCCCCACATCGTCTCAATCGGTCCTTACCACAGAGGAGAGCCACGTCTCAAGATGATCGAAGAGCACAAGTGGAAATACTTGGGCTCTTTGCTTGCAAGGACAGAGCCCAAAGGGTTGACTTTAAAGGACTACTTGAAGGCCTTGGAGCCATTGGAACAAAAAGCTAGAGAGTGTTACTCAGAAAACATAAATCTTAGCACGGATGAATTTTTGGAAATGTTGGTTCTTGATGGTTGCTTTATAATCGAATTGTTTCGAAAATTCGGGCTTTTGGTTCGGTTCGAGCGTGACGATCCTCTTGTCAACATGGTTTGGATAATCCCATTTTTGTCTAGGGATTTTCTTAGGCTTGAAAATCAAATACCCTTCTTTGTTCTTCTGACTTTGTTTGATCTCACAACAATGGAAGAACATAAAGAAAATGGGAAGTCATTGTCGTTGCTTGCCGTGGAATTCTTCAACAACCATATGCAGAGGCCTGATAATGTCATTCAAAAGCACCGCAATCTTACAGGCATGCTCTCTCAAACTTCAATATTTTCTTCGGTACATGAATTTTCTGCCCCTCTATACGTTAACATCGTGTTTAATACCTTGTTTTCAGGTATGCATTTGCTTGACTTGTTACGCTCAAGTGTTATACCCCCAGGCCATGAAGAGCCACGTAGCAGCAACACCATCCCGACACATACCATCCACTGTGTCTCGAAGCTTCGCCGTGCCGGAATCAAGTTCAATCCGGGCAAGGACGAAAGCTTCTTGGTGATCAAATTCAAGCGCGGTGTCATCGAAATGCCGCCTATAACAATCGATGATTTCATGAGCTCGTTCTTACTTAATTGTGTGGCCTATGAGCAATGCCACAAGTCAAGTTCTAAGCACATCACCACCTACGCCACATTGCTCGATTGCCTCGTGAACACATACAAGGATGTTGAGTATTTGAGTGACCGTAACATCATCGAGAATTGCTTTGGGAACGATGGGGAAGTTGCTCGATTTATTAATAATTTGGGGAAGGATGTAGCTTTTGATATGGATAGATGTTACTTGGCTAAGTTGTTCAATGATGTGCATGACTATTATGGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTACACTTATTTTGATACTCCATGGTCCTTCATATCTGCGTTTGCTGCTCTGATTTTGTTGATACTCACCTTGATGCAGACCTTCTACACTGTTTTGAGTTATTACCATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

482

Amino Acids

55.63

Weight (kDa)

6.39

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 54 - 466 1.5e-122 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AccII CGCG 1 cut(s) 990
AciI CCGC 4 cut(s) 17, 712, 990, 1007
AclWI GGATC 2 cut(s) 87, 497
AcsI RAATTY 6 cut(s) 10, 416, 470, 662, 764, 980
AcuI CTGAAG 1 cut(s) 148
AfaI GTAC 3 cut(s) 98, 759, 1334
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 1 cut(s) 856
AluBI AGCT 6 cut(s) 374, 922, 966, 1035, 1238, 1326
AluI AGCT 6 cut(s) 374, 922, 966, 1035, 1238, 1326
Alw21I GWGCWC 2 cut(s) 276, 1037
Alw26I GTCTC 3 cut(s) 226, 260, 919
AlwI GGATC 2 cut(s) 87, 497
AoxI GGCC 5 cut(s) 4, 348, 686, 859, 1056
ApaI GGGCCC 1 cut(s) 8
ApeKI GCWGC 4 cut(s) 137, 156, 879, 1376
ApoI RAATTY 6 cut(s) 10, 416, 470, 662, 764, 980
AseI ATTAAT 1 cut(s) 1215
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 990
AspS9I GGNCC 4 cut(s) 4, 5, 230, 1356
AsuC2I CCSGG 1 cut(s) 951
AsuHPI GGTGA 5 cut(s) 6, 82, 985, 1087, 1390
AsuII TTCGAA 1 cut(s) 466
AsuNHI GCTAGC 1 cut(s) 1322
AvaII GGWCC 2 cut(s) 230, 1356
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 4 cut(s) 8, 296, 318, 1037
BauI CACGAG 1 cut(s) 1124
Bbv12I GWGCWC 2 cut(s) 276, 1037
BbvI GCAGC 4 cut(s) 143, 149, 891, 1363
BccI CCATC 5 cut(s) 57, 434, 896, 911, 1187
BceAI ACGGC 2 cut(s) 641, 912
BciT130I CCWGG 1 cut(s) 858
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 951
BcoDI GTCTC 3 cut(s) 226, 260, 919
BfaI CTAG 3 cut(s) 375, 543, 1323
BisI GCNGC 5 cut(s) 138, 157, 880, 1007, 1377
BlsI GCNGC 5 cut(s) 139, 158, 881, 1008, 1378
Bme1390I CCNGG 2 cut(s) 858, 951
Bme18I GGWCC 2 cut(s) 230, 1356
BmgBI CACGTC 1 cut(s) 254
BmgT120I GGNCC 4 cut(s) 4, 5, 230, 1356
BmiI GGNNCC 2 cut(s) 6, 357
BmrFI CCNGG 2 cut(s) 858, 951
BmsI GCATC 2 cut(s) 168, 1395
BmtI GCTAGC 1 cut(s) 1326
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 2 cut(s) 242, 826
BpuMI CCSGG 1 cut(s) 951
Bsa29I ATCGAT 1 cut(s) 1020
BsaAI YACGTR 1 cut(s) 875
BsaJI CCNNGG 4 cut(s) 351, 657, 856, 1352
Bse3DI GCAATG 3 cut(s) 139, 1073, 1109
BseBI CCWGG 1 cut(s) 858
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 4 cut(s) 351, 657, 856, 1352
BseGI GGATG 6 cut(s) 49, 418, 888, 903, 1147, 1237
BseMI GCAATG 3 cut(s) 139, 1073, 1109
BseMII CTCAG 2 cut(s) 123, 402
BseRI GAGGAG 1 cut(s) 258
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 4 cut(s) 143, 149, 891, 1363
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 5 cut(s) 6, 350, 688, 861, 1058
BshVI ATCGAT 1 cut(s) 1020
BsiHKAI GWGCWC 2 cut(s) 276, 1037
BsiSI CCGG 2 cut(s) 933, 950
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 226, 260, 919
BsmBI CGTCTC 2 cut(s) 226, 260
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 5 cut(s) 6, 350, 688, 861, 1058
Bsp119I TTCGAA 1 cut(s) 466
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 5 cut(s) 8, 276, 296, 318, 1037
Bsp143I GATC 6 cut(s) 79, 105, 264, 502, 601, 975
Bsp19I CCATGG 1 cut(s) 1352
BspACI CCGC 4 cut(s) 17, 712, 990, 1007
BspANI GGCC 5 cut(s) 6, 350, 688, 861, 1058
BspCNI CTCAG 2 cut(s) 122, 401
BspDI ATCGAT 1 cut(s) 1020
BspFNI CGCG 1 cut(s) 990
BspHI TCATGA 1 cut(s) 1029
BspLI GGNNCC 2 cut(s) 6, 357
BspOI GCTAGC 1 cut(s) 1326
BspPI GGATC 2 cut(s) 87, 497
BspQI GCTCTTC 2 cut(s) 264, 861
BspT104I TTCGAA 1 cut(s) 466
BsrDI GCAATG 3 cut(s) 139, 1073, 1109
BssECI CCNNGG 4 cut(s) 351, 657, 856, 1352
BssMI GATC 6 cut(s) 79, 105, 264, 502, 601, 975
BssSI CACGAG 1 cut(s) 1124
BssT1I CCWWGG 2 cut(s) 351, 1352
Bst2BI CACGAG 1 cut(s) 1124
Bst2UI CCWGG 1 cut(s) 858
Bst4CI ACNGT 4 cut(s) 197, 911, 1163, 1423
Bst6I CTCTTC 2 cut(s) 264, 861
BstBAI YACGTR 1 cut(s) 875
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 4 cut(s) 303, 654, 728, 1324
BstDEI CTNAG 7 cut(s) 109, 128, 388, 404, 554, 1086, 1266
BstDSI CCRYGG 2 cut(s) 657, 1352
BstF5I GGATG 6 cut(s) 49, 418, 888, 903, 1147, 1237
BstFNI CGCG 1 cut(s) 990
BstHHI GCGC 1 cut(s) 990
BstKTI GATC 6 cut(s) 82, 108, 267, 505, 604, 978
BstMAI GTCTC 3 cut(s) 226, 260, 919
BstMBI GATC 6 cut(s) 79, 105, 264, 502, 601, 975
BstMWI GCNNNNNNNGC 2 cut(s) 25, 1376
BstNI CCWGG 1 cut(s) 858
BstNSI RCATGY 3 cut(s) 64, 730, 1313
BstSCI CCNGG 2 cut(s) 856, 949
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 4 cut(s) 143, 149, 891, 1363
Bsu15I ATCGAT 1 cut(s) 1020
BsuRI GGCC 5 cut(s) 6, 350, 688, 861, 1058
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 2 cut(s) 657, 1352
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 6 cut(s) 49, 418, 888, 903, 1147, 1237
BtsIMutI CAGTG 2 cut(s) 907, 1419
Cac8I GCNNGC 4 cut(s) 303, 654, 728, 1324
CciI TCATGA 1 cut(s) 1029
CfoI GCGC 1 cut(s) 990
Cfr13I GGNCC 4 cut(s) 4, 5, 230, 1356
ClaI ATCGAT 1 cut(s) 1020
Csp6I GTAC 3 cut(s) 97, 758, 1333
CviQI GTAC 3 cut(s) 97, 758, 1333
DdeI CTNAG 7 cut(s) 109, 128, 388, 404, 554, 1086, 1266
DpnI GATC 6 cut(s) 81, 107, 266, 504, 603, 977
DpnII GATC 6 cut(s) 79, 105, 264, 502, 601, 975
DraI TTTAAA 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 264, 861
EarI CTCTTC 2 cut(s) 264, 861
Ecl136II GAGCTC 1 cut(s) 1035
Eco130I CCWWGG 2 cut(s) 351, 1352
Eco147I AGGCCT 2 cut(s) 350, 688
Eco24I GRGCYC 4 cut(s) 8, 296, 318, 1037
Eco47I GGWCC 2 cut(s) 230, 1356
Eco53kI GAGCTC 1 cut(s) 1035
Eco57I CTGAAG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 1035
EcoRI GAATTC 2 cut(s) 10, 662
EcoRII CCWGG 1 cut(s) 856
EcoT14I CCWWGG 2 cut(s) 351, 1352
EcoT22I ATGCAT 1 cut(s) 822
EcoT38I GRGCYC 4 cut(s) 8, 296, 318, 1037
ErhI CCWWGG 2 cut(s) 351, 1352
Esp3I CGTCTC 2 cut(s) 226, 260
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 1 cut(s) 155
FauNDI CATATG 1 cut(s) 678
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 138, 157, 880, 1007, 1377
FokI GGATG 6 cut(s) 36, 425, 875, 890, 1154, 1244
FriOI GRGCYC 4 cut(s) 8, 296, 318, 1037
Fsp4HI GCNGC 5 cut(s) 138, 157, 880, 1007, 1377
FspBI CTAG 3 cut(s) 375, 543, 1323
GlaI GCGC 1 cut(s) 989
GluI GCNGC 5 cut(s) 138, 157, 880, 1007, 1377
HaeIII GGCC 5 cut(s) 6, 350, 688, 861, 1058
HapII CCGG 2 cut(s) 933, 950
HhaI GCGC 1 cut(s) 990
Hin6I GCGC 1 cut(s) 988
HinP1I GCGC 1 cut(s) 988
HincII GTYRAC 3 cut(s) 327, 514, 787
HindII GTYRAC 3 cut(s) 327, 514, 787
HindIII AAGCTT 2 cut(s) 920, 964
HinfI GANTC 2 cut(s) 168, 936
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 2 cut(s) 933, 950
HphI GGTGA 5 cut(s) 6, 82, 985, 1087, 1390
Hpy166II GTNNAC 5 cut(s) 327, 514, 787, 1129, 1335
Hpy188I TCNGA 5 cut(s) 112, 167, 391, 591, 1383
Hpy188III TCNNGA 8 cut(s) 187, 259, 437, 892, 916, 1030, 1126, 1174
Hpy8I GTNNAC 5 cut(s) 327, 514, 787, 1129, 1335
HpyAV CCTTC 5 cut(s) 340, 586, 1222, 1369, 1423
HpyCH4III ACNGT 4 cut(s) 197, 911, 1163, 1423
HpyCH4IV ACGT 3 cut(s) 253, 783, 874
HpyCH4V TGCA 7 cut(s) 137, 159, 305, 682, 820, 1285, 1408
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 1376
HpyF3I CTNAG 7 cut(s) 109, 128, 388, 404, 554, 1086, 1266
HpySE526I ACGT 3 cut(s) 253, 783, 874
HspAI GCGC 1 cut(s) 988
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 6 cut(s) 79, 105, 264, 502, 601, 975
LguI GCTCTTC 2 cut(s) 264, 861
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 143, 149, 891, 1363
LweI GCATC 2 cut(s) 168, 1395
MaeI CTAG 3 cut(s) 375, 543, 1323
MaeII ACGT 3 cut(s) 253, 783, 874
MaeIII GTNAC 6 cut(s) 383, 497, 834, 1157, 1163, 1256
MalI GATC 6 cut(s) 81, 107, 266, 504, 603, 977
MboI GATC 6 cut(s) 79, 105, 264, 502, 601, 975
MboII GAAGA 7 cut(s) 154, 281, 578, 629, 658, 744, 878
MhlI GDGCHC 5 cut(s) 8, 276, 296, 318, 1037
MlyI GAGTC 1 cut(s) 177
MnlI CCTC 6 cut(s) 160, 236, 516, 678, 786, 1133
Mph1103I ATGCAT 1 cut(s) 822
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 5 cut(s) 332, 786, 798, 1047, 1215
MslI CAYNNNNRTG 3 cut(s) 611, 1001, 1403
MspI CCGG 2 cut(s) 933, 950
MspR9I CCNGG 2 cut(s) 858, 951
MvaI CCWGG 1 cut(s) 858
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 2 cut(s) 25, 1376
NciI CCSGG 1 cut(s) 951
NcoI CCATGG 1 cut(s) 1352
NdeI CATATG 1 cut(s) 678
NdeII GATC 6 cut(s) 79, 105, 264, 502, 601, 975
NheI GCTAGC 1 cut(s) 1322
NlaIV GGNNCC 2 cut(s) 6, 357
NmuCI GTSAC 2 cut(s) 497, 1157
NsiI ATGCAT 1 cut(s) 822
NspI RCATGY 3 cut(s) 64, 730, 1313
NspV TTCGAA 1 cut(s) 466
PaeI GCATGC 1 cut(s) 730
PagI TCATGA 1 cut(s) 1029
PceI AGGCCT 2 cut(s) 350, 688
PciSI GCTCTTC 2 cut(s) 264, 861
PcsI WCGNNNNNNNCGW 4 cut(s) 493, 498, 789, 1122
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 936
PkrI GCNGC 5 cut(s) 139, 158, 881, 1008, 1378
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Ppu21I YACGTR 1 cut(s) 875
PshBI ATTAAT 1 cut(s) 1215
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 1037
Psp6I CCWGG 1 cut(s) 856
PspGI CCWGG 1 cut(s) 856
PspN4I GGNNCC 2 cut(s) 6, 357
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 4 cut(s) 4, 5, 230, 1356
RsaI GTAC 3 cut(s) 98, 759, 1334
RsaNI GTAC 3 cut(s) 97, 758, 1333
RseI CAYNNNNRTG 3 cut(s) 611, 1001, 1403
SacI GAGCTC 1 cut(s) 1037
SapI GCTCTTC 2 cut(s) 264, 861
SaqAI TTAA 5 cut(s) 332, 786, 798, 1047, 1215
SatI GCNGC 5 cut(s) 138, 157, 880, 1007, 1377
Sau3AI GATC 6 cut(s) 79, 105, 264, 502, 601, 975
Sau96I GGNCC 4 cut(s) 4, 5, 230, 1356
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 2 cut(s) 858, 951
SduI GDGCHC 5 cut(s) 8, 276, 296, 318, 1037
SfaNI GCATC 2 cut(s) 168, 1395
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 2 cut(s) 230, 1356
SmiMI CAYNNNNRTG 3 cut(s) 611, 1001, 1403
SmlI CTYRAG 2 cut(s) 257, 841
SmoI CTYRAG 2 cut(s) 257, 841
SphI GCATGC 1 cut(s) 730
SseBI AGGCCT 2 cut(s) 350, 688
SsiI CCGC 4 cut(s) 17, 712, 990, 1007
SspI AATATT 1 cut(s) 747
SspMI CTAG 3 cut(s) 375, 543, 1323
SstI GAGCTC 1 cut(s) 1037
StuI AGGCCT 2 cut(s) 350, 688
StyD4I CCNGG 2 cut(s) 856, 949
StyI CCWWGG 2 cut(s) 351, 1352
TaaI ACNGT 4 cut(s) 197, 911, 1163, 1423
TaiI ACGT 3 cut(s) 256, 786, 877
TaqII GACCGA 1 cut(s) 218
TatI WGTACW 1 cut(s) 1332
TauI GCSGC 1 cut(s) 1009
TfiI GAWTC 1 cut(s) 936
Tru1I TTAA 5 cut(s) 332, 786, 798, 1047, 1215
Tru9I TTAA 5 cut(s) 332, 786, 798, 1047, 1215
TscAI CASTG 2 cut(s) 914, 1426
TseFI GTSAC 2 cut(s) 497, 1157
TseI GCWGC 4 cut(s) 137, 156, 879, 1376
Tsp45I GTSAC 2 cut(s) 497, 1157
TspDTI ATGAA 5 cut(s) 429, 777, 879, 1018, 1351
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 914, 1426
VpaK11BI GGWCC 2 cut(s) 230, 1356
VspI ATTAAT 1 cut(s) 1215
XapI RAATTY 6 cut(s) 10, 416, 470, 662, 764, 980
XceI RCATGY 3 cut(s) 64, 730, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 3 cut(s) 375, 543, 1323
Zsp2I ATGCAT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.